北京华越洋生物
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pCMV-Tag 5B

pCMV-Tag 5B

pCMV-Tag 5B

 

编号

载体名称

北京华越洋生物VECT6082

pCMV-Tag   5B

 

pCMV-Tag5B载体基本信息

载体名称:

pCMV-Tag 5B pCMV-Tag5B

质粒类型:

哺乳动物表达载体

高拷贝/低拷贝:

高拷贝

克隆方法:

多克隆位点;限制性内切酶

启动子:

CMV

载体大小:

4323 bp

5' 测序引物及序列:

CMV ForwardCGCAAATGGGCGGTAGGCGTG

3' 测序引物及序列:

--

载体标签:

c-myc tag (C-末端)

载体抗性:

卡那霉素

筛选标记:

Neomycin

克隆菌株:

XL1-Blue

宿主细胞(系):

--

备注:

c-myc标签含10个氨基酸残基(EQKLISEEDL)
CMV
启动子驱动目的蛋白的高水平表达;
pCMV-Tag 5A,B,C
的差别仅在于读码框(阅读框)处。

稳定性:

瞬表达

组成型/诱导型:

组成型

病毒/非病毒:

非病毒

 

pCMV-Tag5B载体质粒图谱和多克隆位点信息

 

pCMV-Tag5B载体简介

ADDITIONAL MATERIALS REQUIRED

T4 DNA ligase

Taq DNA polymerase

Taq DNA polymerase buffer

TE buffer

CIAP

Falcon 2059 polypropylene tubes (15-ml)

Water baths (37°C and 42°C)

 

XL1-Blue Genotype: recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F proAB lacIqZΔM15 Tn10 (Tetr)].

The XL1-Blue cells supplied with the pCMV-Tag vectors are not competent cells. Refer to Hanahan (1983) for a protocol for producing competent cells.

 

pCMV-Tag5B载体序列

ORIGIN

    1 ATGCATTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA

   61 GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG

  121 CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG

  181 ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA

  241 TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC

  301 CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC

  361 TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC

  421 ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA

  481 TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG

  541 GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA

  601 GCGATTACGC CAAGCTCGAA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG GAGCTCCACC

  661 GCGGTGGCGG CCGCTCTAGC CCGGGCGGAT CCCCCGGGCT GCAGGAATTC GATATCAAGC

  721 TTATCGATAC CGTCGACACT CGAGCAGAAA CTCATCTCTG AAGAGGATCT GTAGGGCCCG

  781 GTACCTTAAT TAATTAAGGT ACCAGGTAAG TGTACCCAAT TCGCCCTATA GTGAGTCGTA

  841 TTACAATTCA CTCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGAGATC

  901 CAATTTTTAA GTGTATAATG TGTTAAACTA CTGATTCTAA TTGTTTGTGT ATTTTAGATT

  961 CACAGTCCCA AGGCTCATTT CAGGCCCCTC AGTCCTCACA GTCTGTTCAT GATCATAATC

 1021 AGCCATACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG

 1081 AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC AGCTTATAAT

 1141 GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT

 1201 TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTAAC GCGTAAATTG TAAGCGTTAA

 1261 TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC

 1321 CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT

 1381 TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA

 1441 AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG

 1501 GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG

 1561 ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC

 1621 TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA

 1681 TGCGCCGCTA CAGGGCGCGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT

 1741 TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA

 1801 AATGCTTCAA TAATATTGAA AAAGGAAGAA TCCTGAGGCG GAAAGAACCA GCTGTGGAAT

 1861 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC

 1921 ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA

 1981 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC

 2041 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT

 2101 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA

 2161 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA GATCGATCAA GAGACAGGAT GAGGATCGTT

 2221 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT

 2281 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT

 2341 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA

 2401 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC

 2461 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG

 2521 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC

 2581 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA

 2641 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA

 2701 CGAAGAACAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC

 2761 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA

 2821 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA

 2881 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAACTT GGCGGCGAAT GGGCTGACCG

 2941 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT

 3001 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC

 3061 AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA

 3121 ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC

 3181 TTCGCCCACC CTAGGGGGAG GCTAACTGAA ACACGGAAGG AGACAATACC GGAAGGAACC

 3241 CGCGCTATGA CGGCAATAAA AAGACAGAAT AAAACGCACG GTGTTGGGTC GTTTGTTCAT

 3301 AAACGCGGGG TTCGGTCCCA GGGCTGGCAC TCTGTCGATA CCCCACCGAG ACCCCATTGG

 3361 GGCCAATACG CCCGCGTTTC TTCCTTTTCC CCACCCCACC CCCCAAGTTC GGGTGAAGGC

 3421 CCAGGGCTCG CAGCCAACGT CGGGGCGGCA GGCCCTGCCA TAGCCTCAGG TTACTCATAT

 3481 ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT

 3541 TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC

 3601 CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC

 3661 TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA

 3721 ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA

 3781 GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT

 3841 CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG

 3901 GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC

 3961 ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA

 4021 TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG

 4081 GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT

 4141 CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG

 4201 CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG

 4261 CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC

 4321 GCC

//

 


其他哺乳动物表达载体:

pEBVHis   A

pcDNA5/TO

pDsRed2-Bid

pNFκB-MetLuc2-Reporter

pGL4.10

pBApo-CMV-neo

pAcGFP1-N1

pEF1α-IRES-DsRed-Express2

pGL4.29

pDsRed-Monomer

pSecTag2   A

pCMV-DsRed-Express2

pGL4.13

pIRES

pGL4.27

pcDNA3.1/NT-GFP-TOPO

pG5   luciferase

pIRES-hrGFP-1a

pGL4.26

pEF1α-IRES-ZsGreen1

pCMV-AD

pDsRed-Express2-N1

pACT

pCMV-Tag   2A

pRevTet-Off

pCMV-Tag   3B

pBIND-Id   Control

pCMV-Tag   5B

pTet-Off

pCRE-hrGFP

pTRE2

pAcGFP1-C   In-Fusion Ready

pTRE2-hygro

pDsRED2-Mito

pRevTRE

p3XFLAG-CMV-14

pVgRxR

pAcGFP1-F

pTK-hyg

p3XFLAG-CMV-8

pOPI3CAT

pAcGFP1-C1

pTRE3G-Luc

pFLAG-CMV-2

pBK-RSV

pAsRed2-C1

pSwitch

pcDNA3.3-TOPO

pIRES2-DsRed2

pAsRed2-N1

pcDNA4/His   C

pcDNA6.2/cLumio-DEST

pCMV-Myc

pAcGFP1-Lam

c-Flag   pcDNA3

pCMV-tdTomato

pCMV-Tag   2C

pAcGFP1-C

pcDNA4/TO/Myc-His   A

pAcGFP1-Mito

pCMV-Tag   5A

pSEAP2-Basic

pcDNA6/myc-His   B

pAcGFP1-N   In-Fusion Ready

pCMV-Tag   3C

pBI-CMV3

pcDNA6/V5-His   B

pDsRed-Monomer-N   In-Fusion Ready

p3XFLAG-CMV-7

pNFkB-DD-tdTomato

pcDNA6.2/nTC-Tag-DEST

pcDNA4/TO/Myc-His   B

p3XFLAG-CMV-9

pcDNA3.1/His   A

pOptiVEC-TOPO

pIRES2-EGFP

pFLAG-CMV-4

pEBVHis   B

pcDNA5/FRT

pcDNA3.1/His   C

pBI-CMV4

pGL4.75

pGL4.30

pcDNA3.1/CT-GFP-TOPO

pcDNA4/His   A

pGL4.20

pGL4.19

pEF1α-IRES-AcGFP1

pcDNA4/myc-His   B

pCMV-SPORT6

pACT-MyoD

pcDNA3.2/V5/GW/D-TOPO

pcDNA4/HisMax   C

pCMV-SPORT6

pCMV-BD

pcDNA4/TO/Myc-His/LacZ

pCMV-Tag   4A

pBIND

pCMV-Tet3G

pcDNA4/HisMax-TOPO

pcDNA6/myc-His   C

pBD-NF-κB

pTet   on advanced

p3XFLAG-CMV-13

pCMV-Tag   2B

pRevTet-On

pTRE-Tight

p3xFLAG-CMV-10

pGRN145

pTet-On

pIND

pFLAG-CMV-3

pCMV-MEK1

pTRE3G

pGene/V5-His   B

pcDNA4/TO/Myc-His   C

pCMV-Tag   3A

pcDNA4/TO

pOPRSVI

pcDNA6.2/C-YFP-DEST

pCMVLacI

pcDNA4/His   B

pcDNA4/HisMax   A

pcDNA6.2/cTC-Tag-DEST

pBI-CMV1

pcDNA4/HisMax   B

pIRESpuro3

pcDNA6.2/nGeneBLAzer-DEST

pEF1α-AcGFP1-N1

pcDNA4/myc-His   C

pIRESneo3

pCRE-MetLuc2-Reporter

pCMV-LacZ

pcDNA3.1/His   B

pIRESneo2

pEF1α-DsRed-Express2

pCMV-Tag   4B

pcDNA6/V5-His   C

pcDNA4/myc-His   A

pDsRed-Express-C1

pCMV-Tag   5C

pCHO1.0

pCMV-PKA

pEF1α-DsRed-Monomer-N1

plRES2-ZsGreen1

pGL3-Promoter

pAcGFP1-N3

pDD-AmCyan1   Reporter

p3XFLAG-CMV-7.1

pCMV-MEKK1

pcDNA5/FRT/TO

pCRE-DD-AmCyan1

pFLAG-CMV-5a

pFLAG-CMV2

pBApo-CMV-Pur

pIRES2-DsRed-Express2

pBudCE4.1

pAcGFP1-C2

pBApo-EF1α-pur

pDsRed-Express-N1

pREP4

ptdTomato-C1

ptdTomato-N1

pcDNA6.2/nLumio-DEST

pBApo-CMV

pCRE-DD-tdTomato

pAcGFP1-Golgi

pcDNA6/myc-His   A

pIRES2-AcGFP1

pAcGFP1-Hyg-C1

pAcGFP1-p53

pcDNA6/V5-His   A

pIREShyg3

pAcGFP1-Mem

pAcGFP1-Actin

pcDNA5/FRT/TO-TOPO

pcDNA6/TR

pAcGFP1-C3

pBI-CMV2

pcDNA6.2/V5/GW/D-TOPO

pDsRed-Express2-C1

pTT5

pEF1α-tdTomato

pcDNA6.2/cGeneBLAzer-DEST

pBI-CMV5

pSEAP2-Control

pEF1α-tdTomato

pcDNA6.2/nGeneBLAzer-GW/D-TOPO

pAmCyan1-C1

pSEAP2-Control