北京华越洋生物
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首页    载体及质粒    pTRE-Tight
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pTRE-Tight

pTRE-Tight

pTRE-Tight

 

编号

载体名称

北京华越洋生物VECT6040

pTRE-Tight

pTRE-Tight载体基本信息

载体名称:

pTRE-Tight

质粒类型:

哺乳细胞表达;四环素调控载体

高拷贝/低拷贝:

--

启动子:

--

克隆方法:

多克隆位点,限制性内切酶

载体大小:

2605bp

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

--

载体抗性:

氨苄青霉素(Ampicillin)

筛选标记:

--

备注:

--

稳定性:

--

组成型:

诱导型

病毒/非病毒:

--

pTRE-Tight载体质粒图谱和多克隆位点信息

 


 

 

pTRE-Tight载体序列

ORIGIN

    1 CTCGAGTTTA CTCCCTATCA GTGATAGAGA ACGTATGTCG AGTTTACTCC CTATCAGTGA

   61 TAGAGAACGA TGTCGAGTTT ACTCCCTATC AGTGATAGAG AACGTATGTC GAGTTTACTC

  121 CCTATCAGTG ATAGAGAACG TATGTCGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG

  181 TCGAGTTTAT CCCTATCAGT GATAGAGAAC GTATGTCGAG TTTACTCCCT ATCAGTGATA

  241 GAGAACGTAT GTCGAGGTAG GCGTGTACGG TGGGAGGCCT ATATAAGCAG AGCTCGTTTA

  301 GTGAACCGTC AGATCGCCTG GAGAATTCGA GCTCGGTACC CGGGGATCCT CTAGTCAGCT

  361 GACGCGTGCT AGCGCGGCCG CATCGATAAG CTTGTCGACG ATATCTCTAG AGGATCATAA

  421 TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA AAACCTCCCA CACCTCCCCC

  481 TGAACCTGAA ACATAAAATG AATGCAATTG TTGTTGTTAA CTTGTTTATT GCAGCTTATA

  541 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC

  601 CTCGAGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT

  661 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA

  721 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT

  781 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT

  841 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC

  901 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA

  961 GCGTGGCGCT TTCTCAATGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT

 1021 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA

 1081 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG

 1141 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC

 1201 CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA

 1261 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG

 1321 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT

 1381 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG

 1441 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA

 1501 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG

 1561 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG

 1621 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC

 1681 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG

 1741 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG

 1801 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG

 1861 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT

 1921 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC

 1981 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC

 2041 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA

 2101 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC

 2161 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT

 2221 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC

 2281 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA

 2341 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA

 2401 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT

 2461 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA

 2521 AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC

 2581 GTATCACGAG GCCCTTTCGT CTTCA

//

 

其他哺乳动物表达载体:

pEBVHis   A

pcDNA5/TO

pDsRed2-Bid

pNFκB-MetLuc2-Reporter

pGL4.10

pBApo-CMV-neo

pAcGFP1-N1

pEF1α-IRES-DsRed-Express2

pGL4.29

pDsRed-Monomer

pSecTag2   A

pCMV-DsRed-Express2

pGL4.13

pIRES

pGL4.27

pcDNA3.1/NT-GFP-TOPO

pG5   luciferase

pIRES-hrGFP-1a

pGL4.26

pEF1α-IRES-ZsGreen1

pCMV-AD

pDsRed-Express2-N1

pACT

pCMV-Tag   2A

pRevTet-Off

pCMV-Tag   3B

pBIND-Id   Control

pCMV-Tag   5B

pTet-Off

pCRE-hrGFP

pTRE2

pAcGFP1-C   In-Fusion Ready

pTRE2-hygro

pDsRED2-Mito

pRevTRE

p3XFLAG-CMV-14

pVgRxR

pAcGFP1-F

pTK-hyg

p3XFLAG-CMV-8

pOPI3CAT

pAcGFP1-C1

pTRE3G-Luc

pFLAG-CMV-2

pBK-RSV

pAsRed2-C1

pSwitch

pcDNA3.3-TOPO

pIRES2-DsRed2

pAsRed2-N1

pcDNA4/His   C

pcDNA6.2/cLumio-DEST

pCMV-Myc

pAcGFP1-Lam

c-Flag   pcDNA3

pCMV-tdTomato

pCMV-Tag   2C

pAcGFP1-C

pcDNA4/TO/Myc-His   A

pAcGFP1-Mito

pCMV-Tag   5A

pSEAP2-Basic

pcDNA6/myc-His   B

pAcGFP1-N   In-Fusion Ready

pCMV-Tag   3C

pBI-CMV3

pcDNA6/V5-His   B

pDsRed-Monomer-N   In-Fusion Ready

p3XFLAG-CMV-7

pNFkB-DD-tdTomato

pcDNA6.2/nTC-Tag-DEST

pcDNA4/TO/Myc-His   B

p3XFLAG-CMV-9

pcDNA3.1/His   A

pOptiVEC-TOPO

pIRES2-EGFP

pFLAG-CMV-4

pEBVHis   B

pcDNA5/FRT

pcDNA3.1/His   C

pBI-CMV4

pGL4.75

pGL4.30

pcDNA3.1/CT-GFP-TOPO

pcDNA4/His   A

pGL4.20

pGL4.19

pEF1α-IRES-AcGFP1

pcDNA4/myc-His   B

pCMV-SPORT6

pACT-MyoD

pcDNA3.2/V5/GW/D-TOPO

pcDNA4/HisMax   C

pCMV-SPORT6

pCMV-BD

pcDNA4/TO/Myc-His/LacZ

pCMV-Tag   4A

pBIND

pCMV-Tet3G

pcDNA4/HisMax-TOPO

pcDNA6/myc-His   C

pBD-NF-κB

pTet   on advanced

p3XFLAG-CMV-13

pCMV-Tag   2B

pRevTet-On

pTRE-Tight

p3xFLAG-CMV-10

pGRN145

pTet-On

pIND

pFLAG-CMV-3

pCMV-MEK1

pTRE3G

pGene/V5-His   B

pcDNA4/TO/Myc-His   C

pCMV-Tag   3A

pcDNA4/TO

pOPRSVI

pcDNA6.2/C-YFP-DEST

pCMVLacI

pcDNA4/His   B

pcDNA4/HisMax   A

pcDNA6.2/cTC-Tag-DEST

pBI-CMV1

pcDNA4/HisMax   B

pIRESpuro3

pcDNA6.2/nGeneBLAzer-DEST

pEF1α-AcGFP1-N1

pcDNA4/myc-His   C

pIRESneo3

pCRE-MetLuc2-Reporter

pCMV-LacZ

pcDNA3.1/His   B

pIRESneo2

pEF1α-DsRed-Express2

pCMV-Tag   4B

pcDNA6/V5-His   C

pcDNA4/myc-His   A

pDsRed-Express-C1

pCMV-Tag   5C

pCHO1.0

pCMV-PKA

pEF1α-DsRed-Monomer-N1

plRES2-ZsGreen1

pGL3-Promoter

pAcGFP1-N3

pDD-AmCyan1   Reporter

p3XFLAG-CMV-7.1

pCMV-MEKK1

pcDNA5/FRT/TO

pCRE-DD-AmCyan1

pFLAG-CMV-5a

pFLAG-CMV2

pBApo-CMV-Pur

pIRES2-DsRed-Express2

pBudCE4.1

pAcGFP1-C2

pBApo-EF1α-pur

pDsRed-Express-N1

pREP4

ptdTomato-C1

ptdTomato-N1

pcDNA6.2/nLumio-DEST

pBApo-CMV

pCRE-DD-tdTomato

pAcGFP1-Golgi

pcDNA6/myc-His   A

pIRES2-AcGFP1

pAcGFP1-Hyg-C1

pAcGFP1-p53

pcDNA6/V5-His   A

pIREShyg3

pAcGFP1-Mem

pAcGFP1-Actin

pcDNA5/FRT/TO-TOPO

pcDNA6/TR

pAcGFP1-C3

pBI-CMV2

pcDNA6.2/V5/GW/D-TOPO

pDsRed-Express2-C1

pTT5

pEF1α-tdTomato

pcDNA6.2/cGeneBLAzer-DEST

pBI-CMV5

pSEAP2-Control

pAmCyan1-C1

pcDNA6.2/nGeneBLAzer-GW/D-TOPO