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pIND

编号	载体名称
北京华越洋生物VECT6039	pIND

pIND载体基本信息

载体名称:	pIND
质粒类型:	哺乳细胞表达载体；蜕皮激素诱导表达系统载体
高拷贝/低拷贝:	--
启动子:	--
克隆方法:	多克隆位点，限制性内切酶
载体大小:	5023bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	氨苄青霉素
筛选标记:	新霉素（Neomycin）
备注:	--
稳定性:	--
组成型:	--
病毒/非病毒:	--

pIND载体质粒图谱和多克隆位点信息

pIND载体序列

ORIGIN

1 AGATCTCGGC CGCATATTAA GTGCATTGTT CTCGATACCG CTAAGTGCAT TGTTCTCGTT

61 AGCTCGATGG ACAAGTGCAT TGTTCTCTTG CTGAAAGCTC GATGGACAAG TGCATTGTTC

121 TCTTGCTGAA AGCTCGATGG ACAAGTGCAT TGTTCTCTTG CTGAAAGCTC AGTACCCGGG

181 AGTACCCTCG ACCGCCGGAG TATAAATAGA GGCGCTTCGT CTACGGAGCG ACAATTCAAT

241 TCAAACAAGC AAAGTGAACA CGTCGCTAAG CGAAAGCTAA GCAAATAAAC AAGCGCAGCT

301 GAACAAGCTA AACAATCTGC AGTAAAGTGC AAGTTAAAGT GAATCAATTA AAAGTAACCA

361 GCAACCAAGT AAATCAACTG CAACTACTGA AATCTGCCAA GAAGTAATTA TTGAATACAA

421 GAAGAGAACT CTGAATACTT TCAACAAGTT ACCGAGAAAG AAGAACTCAC ACACAGCTAG

481 CGTTTAAACT TAAGCTTGGT ACCGAGCTCG GATCCACTAG TCCAGTGTGG TGGAATTCTG

541 CAGATATCCA GCACAGTGGC GGCCGCTCGA GTCTAGAGGG CCCGTTTAAA CCCGCTGATC

601 AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC

661 CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC

721 GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG

781 GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA

841 GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT

901 AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC

961 GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA

1021 AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC

1081 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT

1141 TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC

1201 AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC

1261 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT

1321 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC

1381 ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA

1441 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC

1501 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT

1561 TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA

1621 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC

1681 GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC

1741 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA

1801 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT

1861 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG

1921 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA

1981 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT

2041 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG

2101 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG

2161 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC

2221 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT

2281 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC

2341 TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT

2401 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT

2461 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC

2521 TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA

2581 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA

2641 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG

2701 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT

2761 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC

2821 CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT

2881 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG

2941 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT

3001 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT

3061 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC

3121 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG

3181 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG

3241 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC

3301 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG

3361 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT

3421 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG

3481 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT

3541 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC

3601 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT

3661 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC

3721 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA

3781 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT

3841 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC

3901 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT

3961 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC

4021 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG

4081 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG

4141 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC

4201 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA

4261 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG

4321 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT

4381 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA

4441 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG

4501 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA

4561 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT

4621 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA

4681 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT

4741 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT

4801 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA

4861 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT

4921 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC

4981 GCACATTTCC CCGAAAAGTG CCACCTGACG TCGACGGATC GGG

其他哺乳动物表达载体：

pEBVHis A	pcDNA5/TO	pDsRed2-Bid	pNFκB-MetLuc2-Reporter
pGL4.10	pBApo-CMV-neo	pAcGFP1-N1	pEF1α-IRES-DsRed-Express2
pGL4.29	pDsRed-Monomer	pSecTag2 A	pCMV-DsRed-Express2
pGL4.13	pIRES	pGL4.27	pcDNA3.1/NT-GFP-TOPO
pG5 luciferase	pIRES-hrGFP-1a	pGL4.26	pEF1α-IRES-ZsGreen1
pCMV-AD	pDsRed-Express2-N1	pACT	pCMV-Tag 2A
pRevTet-Off	pCMV-Tag 3B	pBIND-Id Control	pCMV-Tag 5B
pTet-Off	pCRE-hrGFP	pTRE2	pAcGFP1-C In-Fusion Ready
pTRE2-hygro	pDsRED2-Mito	pRevTRE	p3XFLAG-CMV-14
pVgRxR	pAcGFP1-F	pTK-hyg	p3XFLAG-CMV-8
pOPI3CAT	pAcGFP1-C1	pTRE3G-Luc	pFLAG-CMV-2
pBK-RSV	pAsRed2-C1	pSwitch	pcDNA3.3-TOPO
pIRES2-DsRed2	pAsRed2-N1	pcDNA4/His C	pcDNA6.2/cLumio-DEST
pCMV-Myc	pAcGFP1-Lam	c-Flag pcDNA3	pCMV-tdTomato
pCMV-Tag 2C	pAcGFP1-C	pcDNA4/TO/Myc-His A	pAcGFP1-Mito
pCMV-Tag 5A	pSEAP2-Basic	pcDNA6/myc-His B	pAcGFP1-N In-Fusion Ready
pCMV-Tag 3C	pBI-CMV3	pcDNA6/V5-His B	pDsRed-Monomer-N In-Fusion Ready
p3XFLAG-CMV-7	pNFkB-DD-tdTomato	pcDNA6.2/nTC-Tag-DEST	pcDNA4/TO/Myc-His B
p3XFLAG-CMV-9	pcDNA3.1/His A	pOptiVEC-TOPO	pIRES2-EGFP
pFLAG-CMV-4	pEBVHis B	pcDNA5/FRT	pcDNA3.1/His C
pBI-CMV4	pGL4.75	pGL4.30	pcDNA3.1/CT-GFP-TOPO
pcDNA4/His A	pGL4.20	pGL4.19	pEF1α-IRES-AcGFP1
pcDNA4/myc-His B	pCMV-SPORT6	pACT-MyoD	pcDNA3.2/V5/GW/D-TOPO
pcDNA4/HisMax C	pCMV-SPORT6	pCMV-BD	pcDNA4/TO/Myc-His/LacZ
pCMV-Tag 4A	pBIND	pCMV-Tet3G	pcDNA4/HisMax-TOPO
pcDNA6/myc-His C	pBD-NF-κB	pTet on advanced	p3XFLAG-CMV-13
pCMV-Tag 2B	pRevTet-On	pTRE-Tight	p3xFLAG-CMV-10
pGRN145	pTet-On	pIND	pFLAG-CMV-3
pCMV-MEK1	pTRE3G	pGene/V5-His B	pcDNA4/TO/Myc-His C
pCMV-Tag 3A	pcDNA4/TO	pOPRSVI	pcDNA6.2/C-YFP-DEST
pCMVLacI	pcDNA4/His B	pcDNA4/HisMax A	pcDNA6.2/cTC-Tag-DEST
pBI-CMV1	pcDNA4/HisMax B	pIRESpuro3	pcDNA6.2/nGeneBLAzer-DEST
pEF1α-AcGFP1-N1	pcDNA4/myc-His C	pIRESneo3	pCRE-MetLuc2-Reporter
pCMV-LacZ	pcDNA3.1/His B	pIRESneo2	pEF1α-DsRed-Express2
pCMV-Tag 4B	pcDNA6/V5-His C	pcDNA4/myc-His A	pDsRed-Express-C1
pCMV-Tag 5C	pCHO1.0	pCMV-PKA	pEF1α-DsRed-Monomer-N1
plRES2-ZsGreen1	pGL3-Promoter	pAcGFP1-N3	pDD-AmCyan1 Reporter
p3XFLAG-CMV-7.1	pCMV-MEKK1	pcDNA5/FRT/TO	pCRE-DD-AmCyan1
pFLAG-CMV-5a	pFLAG-CMV2	pBApo-CMV-Pur	pIRES2-DsRed-Express2
pBudCE4.1	pAcGFP1-C2	pBApo-EF1α-pur	pDsRed-Express-N1
pREP4	ptdTomato-C1	ptdTomato-N1	pcDNA6.2/nLumio-DEST
pBApo-CMV	pCRE-DD-tdTomato	pAcGFP1-Golgi	pcDNA6/myc-His A
pIRES2-AcGFP1	pAcGFP1-Hyg-C1	pAcGFP1-p53	pcDNA6/V5-His A
pIREShyg3	pAcGFP1-Mem	pAcGFP1-Actin	pcDNA5/FRT/TO-TOPO
pcDNA6/TR	pAcGFP1-C3	pBI-CMV2	pcDNA6.2/V5/GW/D-TOPO
pDsRed-Express2-C1	pTT5	pEF1α-tdTomato	pcDNA6.2/cGeneBLAzer-DEST
pBI-CMV5	pSEAP2-Control	pAmCyan1-C1	pcDNA6.2/nGeneBLAzer-GW/D-TOPO