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pCMV-MEKK1

pCMV-MEKK1

pCMV-MEKK1

 

编号

载体名称

北京华越洋生物VECT6118

pCMV-MEKK1

 

pCMV-MEKK1载体基本信息

载体名称:

pCMV-MEKK1

质粒类型:

哺乳动物表达载体;基因表达载体

高拷贝/低拷贝:

高拷贝

克隆方法:

限制性内切酶,多克隆位点

启动子:

CMV

载体大小:

5 kb

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

载体抗性:

卡那霉素

筛选标记:

Neomycin

克隆菌株:

TOP10F′, JM109 and INVaF′

宿主细胞(系):

常规细胞系,如293Hela

备注:

pCMV-MEKK1载体是蛋白激酶MEKK1的表达载体;
  CMV
启动子驱动MEKK1基因的过表达;
  MEKK1
蛋白激酶是JNK/SAPK信号通路的应答激酶。

产品目录号:

631927

稳定性:

瞬表达 稳表达

组成型/诱导型:

组成型

病毒/非病毒:

非病毒

 

pCMV-MEKK1载体质粒图谱和多克隆位点信息

 

pCMV-MEKK1载体使用说明

 

pCMV-MEKK1 is designed for the constitutive expression of MEK Kinase 1 (MEKK1) in mammalian cells. It can also be used to study the effects of a given stimulus on the JNK/SAPK pathway. In conjunction with Clontech’s In Vivo Kinase Assay Kits, pCMV-MEKK1 is an ideal positive control for studying a gene or molecule and its affect on kinase activation in the JNK/SAPK pathway.

 

MEKK1 mediates cellular responses to diverse stimuli, such as cytokines, growth factors, and other stressors via activation of JNK/SAPK signaling pathway. It has also been reported that MEKK1 is a possible regulator in signal transduction pathways involving apoptosis (1). To a lesser extent, MEKK1 can also activate the ERK pathway via phosphorylation of MEK (2, 3); however, it is more efficient in activating the JNK/SAPK than ERK (4, 5).

pCMV-MEKK1 contains a gene encoding MEKK1 (mouse) that is driven by the human cytomegalovirus(CMV) promoter. SV40 polyadenylation signals downstream of the MEKK1 gene direct proper processing of the 3’ end of the mRNA. The vector backbone also contains an SV40 origin for replication in mammalian cells expressing the SV40 T-antigen. A neomycin resistance cassette (Neor)—consisting of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the Herpes simplex virus thymidine kinase (HSV TK) gene—allows stably transfected eukaryotic cells to be selected using G418(6). A bacterial promoter upstream of this cassette expresses kanamycin resistance in E. coli. The pCMV-MEKK1 backbone also provides a pUC origin of replication for propagation in E.coli and an f1 origin for single-stranded DNA production. The recombinant vector can be transfected into mammalian cells using any standard method.

 

Propagation in E. coli:

 Suitable host strains: DH5α, HB101, and other general purpose strains. Single-stranded DNA production requires

a host containing an F plasmid such as JM109 or XL1-Blue.

 Selectable marker: plasmid confers resistance to kanamycin (50 μg/ml) to E. coli hosts.

 E. coli replication origin: pUC

 Copy number: 500

 Plasmid incompatibility group: pMB1/ColE1

 

其他哺乳动物表达载体:

pEBVHis   A

pcDNA5/TO

pDsRed2-Bid

pNFκB-MetLuc2-Reporter

pGL4.10

pBApo-CMV-neo

pAcGFP1-N1

pEF1α-IRES-DsRed-Express2

pGL4.29

pDsRed-Monomer

pSecTag2   A

pCMV-DsRed-Express2

pGL4.13

pIRES

pGL4.27

pcDNA3.1/NT-GFP-TOPO

pG5   luciferase

pIRES-hrGFP-1a

pGL4.26

pEF1α-IRES-ZsGreen1

pCMV-AD

pDsRed-Express2-N1

pACT

pCMV-Tag   2A

pRevTet-Off

pCMV-Tag   3B

pBIND-Id   Control

pCMV-Tag   5B

pTet-Off

pCRE-hrGFP

pTRE2

pAcGFP1-C   In-Fusion Ready

pTRE2-hygro

pDsRED2-Mito

pRevTRE

p3XFLAG-CMV-14

pVgRxR

pAcGFP1-F

pTK-hyg

p3XFLAG-CMV-8

pOPI3CAT

pAcGFP1-C1

pTRE3G-Luc

pFLAG-CMV-2

pBK-RSV

pAsRed2-C1

pSwitch

pcDNA3.3-TOPO

pIRES2-DsRed2

pAsRed2-N1

pcDNA4/His   C

pcDNA6.2/cLumio-DEST

pCMV-Myc

pAcGFP1-Lam

c-Flag   pcDNA3

pCMV-tdTomato

pCMV-Tag   2C

pAcGFP1-C

pcDNA4/TO/Myc-His   A

pAcGFP1-Mito

pCMV-Tag   5A

pSEAP2-Basic

pcDNA6/myc-His   B

pAcGFP1-N   In-Fusion Ready

pCMV-Tag   3C

pBI-CMV3

pcDNA6/V5-His   B

pDsRed-Monomer-N   In-Fusion Ready

p3XFLAG-CMV-7

pNFkB-DD-tdTomato

pcDNA6.2/nTC-Tag-DEST

pcDNA4/TO/Myc-His   B

p3XFLAG-CMV-9

pcDNA3.1/His   A

pOptiVEC-TOPO

pIRES2-EGFP

pFLAG-CMV-4

pEBVHis   B

pcDNA5/FRT

pcDNA3.1/His   C

pBI-CMV4

pGL4.75

pGL4.30

pcDNA3.1/CT-GFP-TOPO

pcDNA4/His   A

pGL4.20

pGL4.19

pEF1α-IRES-AcGFP1

pcDNA4/myc-His   B

pCMV-SPORT6

pACT-MyoD

pcDNA3.2/V5/GW/D-TOPO

pcDNA4/HisMax   C

pCMV-SPORT6

pCMV-BD

pcDNA4/TO/Myc-His/LacZ

pCMV-Tag   4A

pBIND

pCMV-Tet3G

pcDNA4/HisMax-TOPO

pcDNA6/myc-His   C

pBD-NF-κB

pTet   on advanced

p3XFLAG-CMV-13

pCMV-Tag   2B

pRevTet-On

pTRE-Tight

p3xFLAG-CMV-10

pGRN145

pTet-On

pIND

pFLAG-CMV-3

pCMV-MEK1

pTRE3G

pGene/V5-His   B

pcDNA4/TO/Myc-His   C

pCMV-Tag   3A

pcDNA4/TO

pOPRSVI

pcDNA6.2/C-YFP-DEST

pCMVLacI

pcDNA4/His   B

pcDNA4/HisMax   A

pcDNA6.2/cTC-Tag-DEST

pBI-CMV1

pcDNA4/HisMax   B

pIRESpuro3

pcDNA6.2/nGeneBLAzer-DEST

pEF1α-AcGFP1-N1

pcDNA4/myc-His   C

pIRESneo3

pCRE-MetLuc2-Reporter

pCMV-LacZ

pcDNA3.1/His   B

pIRESneo2

pEF1α-DsRed-Express2

pCMV-Tag   4B

pcDNA6/V5-His   C

pcDNA4/myc-His   A

pDsRed-Express-C1

pCMV-Tag   5C

pCHO1.0

pCMV-PKA

pEF1α-DsRed-Monomer-N1

plRES2-ZsGreen1

pGL3-Promoter

pAcGFP1-N3

pDD-AmCyan1   Reporter

p3XFLAG-CMV-7.1

pCMV-MEKK1

pcDNA5/FRT/TO

pCRE-DD-AmCyan1

pFLAG-CMV-5a

pFLAG-CMV2

pBApo-CMV-Pur

pIRES2-DsRed-Express2

pBudCE4.1

pAcGFP1-C2

pBApo-EF1α-pur

pDsRed-Express-N1

pREP4

ptdTomato-C1

ptdTomato-N1

pcDNA6.2/nLumio-DEST

pBApo-CMV

pCRE-DD-tdTomato

pAcGFP1-Golgi

pcDNA6/myc-His   A

pIRES2-AcGFP1

pAcGFP1-Hyg-C1

pAcGFP1-p53

pcDNA6/V5-His   A

pIREShyg3

pAcGFP1-Mem

pAcGFP1-Actin

pcDNA5/FRT/TO-TOPO

pcDNA6/TR

pAcGFP1-C3

pBI-CMV2

pcDNA6.2/V5/GW/D-TOPO

pDsRed-Express2-C1

pTT5

pEF1α-tdTomato

pcDNA6.2/cGeneBLAzer-DEST

pBI-CMV5

pSEAP2-Control

pEF1α-tdTomato

pcDNA6.2/nGeneBLAzer-GW/D-TOPO

pAmCyan1-C1

pSEAP2-Control