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pCMV-Tet3G

编号	载体名称
北京华越洋生物VECT6042	pCMV-Tet3G

pCMV-Tet3G载体基本信息

载体名称:	pCMV-Tet3G
质粒类型:	哺乳细胞表达；四环素调控载体
高拷贝/低拷贝:	--
启动子:	PCMV IE
克隆方法:	多克隆位点，限制性内切酶
载体大小:	7139bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	氨苄青霉素和卡纳霉素
筛选标记:	新霉素
备注:	--
稳定性:	--
组成型:	--
病毒/非病毒:	非病毒

pCMV-Tet3G载体质粒图谱和多克隆位点信息

pCMV-Tet3G载体序列

ORIGIN

1 CTCGAGGAGC TTGGCCCATT GCATACGTTG TATCCATATC ATAATATGTA CATTTATATT

61 GGCTCATGTC CAACATTACC GCCATGTTGA CATTGATTAT TGACTAGTTA TTAATAGTAA

121 TCAATTACGG GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG

181 GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG

241 TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA

301 CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT

361 GACGTCAATG ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC

421 TTTCCTACTT GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT

481 TGGCAGTACA TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC

541 CCCATTGACG TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT

601 CGTAACAACT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT

661 ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT

721 GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGCG GCCCCGAATT CACCATGTCT

781 AGACTGGACA AGAGCAAAGT CATAAACTCT GCTCTGGAAT TACTCAATGG AGTCGGTATC

841 GAAGGCCTGA CGACAAGGAA ACTCGCTCAA AAGCTGGGAG TTGAGCAGCC TACCCTGTAC

901 TGGCACGTGA AGAACAAGCG GGCCCTGCTC GATGCCCTGC CAATCGAGAT GCTGGACAGG

961 CATCATACCC ACTCCTGCCC CCTGGAAGGC GAGTCATGGC AAGACTTTCT GCGGAACAAC

1021 GCCAAGTCAT ACCGCTGTGC TCTCCTCTCA CATCGCGACG GGGCTAAAGT GCATCTCGGC

1081 ACCCGCCCAA CAGAGAAACA GTACGAAACC CTGGAAAATC AGCTCGCGTT CCTGTGTCAG

1141 CAAGGCTTCT CCCTGGAGAA CGCACTGTAC GCTCTGTCCG CCGTGGGCCA CTTTACACTG

1201 GGCTGCGTAT TGGAGGAACA GGAGCATCAA GTAGCAAAAG AGGAAAGAGA GACACCTACC

1261 ACCGATTCTA TGCCCCCACT TCTGAAACAA GCAATTGAGC TGTTCGACCG GCAGGGAGCC

1321 GAACCTGCCT TCCTTTTCGG CCTGGAACTA ATCATATGTG GCCTGGAGAA ACAGCTAAAG

1381 TGCGAAAGCG GCGGGCCGAC CGACGCCCTT GACGATTTTG ACTTAGACAT GCTCCCAGCC

1441 GATGCCCTTG ACGACTTTGA CCTTGATATG CTGCCTGCTG ACGCTCTTGA CGATTTTGAC

1501 CTTGACATGC TCCCCGGGTA ACTAAGTAAG GATCCAGACA TGATAAGATA CATTGATGAG

1561 TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT

1621 GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC

1681 ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC

1741 CTCTACAAAT GTGGTATGGC TGATTATGAT CCTGCAAGCC TCGTCGTCCT GGCCGGACCA

1801 CGCTATCTGT GCAAGGTCCC CGGCCCCGGA CGCGCGCTCC ATGAGCAGAG CGCCCGCCGC

1861 CGAGGCGAAG ACTCGGGCGG CGCCCTGCCC GTCCCACCAG GTCAACAGGC GGTAACCGGC

1921 CTCTTCATCG GGAATGCGCG CGACCTTCAG CATCGCCGGC ATGTCCCCCT GGCGGACGGG

1981 AAGTATCCAG CTCGACCAAG CTTGGCGAGA TTTTCAGGAG CTAAGGAAGC TAAAATGGAG

2041 AAAAAAATCA CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA AGAACATTTT

2101 GAGGCATTTC AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT GCATTAATGA

2161 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC

2221 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG

2281 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC

2341 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC

2401 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA

2461 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC

2521 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT

2581 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG

2641 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC

2701 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA

2761 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT

2821 AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT

2881 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG

2941 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG

3001 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA

3061 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA

3121 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG

3181 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA

3241 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG

3301 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT

3361 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT

3421 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC

3481 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA

3541 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT

3601 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC

3661 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA

3721 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA CACGGGATAA TACCGCGCCA

3781 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA

3841 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT

3901 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC

3961 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA

4021 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT

4081 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC

4141 TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT

4201 CGTCTTCACT CGAGGTCGAG GGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA

4261 ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT

4321 TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT

4381 ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA

4441 TGTGGTATGG CTGATTATGA TCTCTAGTCA AGGCACTATA CATCAAATAT TCCTTATTAA

4501 CCCCTTTACA AATTAAAAAG CTAAAGGTAC ACAATTTTTG AGCATAGTTA TTAATAGCAG

4561 ACACTCTATG CCTGTGTGGA GTAAGAAAAA ACAGTATGTT ATGATTATAA CTGTTATGCC

4621 TACTTATAAA GGTTACAGAA TATTTTTCCA TAATTTTCTT GTATAGCAGT GCAGCTTTTT

4681 CCTTTGTGGT GTAAATAGCA AAGCAAGCAA GAGTTCTATT ACTAAACACA GCATGACTCA

4741 AAAAACTTAG CAATTCTGAA GGAAAGTCCT TGGGGTCTTC TACCTTTCTC TTCTTTTTTG

4801 GAGGAGTAGA ATGTTGAGAG TCAGCAGTAG CCTCATCATC ACTAGATGGC ATTTCTTCTG

4861 AGCAAAACAG GTTTTCCTCA TTAAAGGCAT TCCACCACTG CTCCCATTCA TCAGTTCCAT

4921 AGGTTGGAAT CTAAAATACA CAAACAATTA GAATCAGTAG TTTAACACAT TATACACTTA

4981 AAAATTTTAT ATTTACCTTA GAGCTTTAAA TCTCTGTAGG TAGTTTGTCC AATTATGTCA

5041 CACCACAGAA GTAAGGTTCC TTCACAAAGA TCCGGACCAA AGCGGCCATC GTGCCTCCCC

5101 ACTCCTGCAG TTCGGGGGCA TGGATGCGCG GATAGCCGCT GCTGGTTTCC TGGATGCCGA

5161 CGGATTTGCA CTGCCGGTAG AACTCCGCGA GGTCGTCCAG CCTCAGGCAG CAGCTGAACC

5221 AACTCGCGAG GGGATCGAGC CCGGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT

5281 GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG

5341 CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA

5401 ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA

5461 ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC

5521 TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG CGGTCCGCCA CACCCAGCCG

5581 GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC

5641 ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG CGCGCCTTGA GCCTGGCGAA

5701 CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC

5761 GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA

5821 GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC

5881 GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA

5941 GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC

6001 CAGCCACGAT AGCCGCGCTG CCTCGTCCTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT

6061 CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA

6121 GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA

6181 ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC

6241 AGATCTTGAT CCCCTGCGCC ATCAGATCCT TGGCGGCAAG AAAGCCATCC AGTTTACTTT

6301 GCAGGGCTTC CCAACCTTAC CAGAGGGCGC CCCAGCTGGC AATTCCGGTT CGCTTGCTGT

6361 CCATAAAACC GCCCAGTCTA GCTATCGCCA TGTAAGCCCA CTGCAAGCTA CCTGCTTTCT

6421 CTTTGCGCTT GCGTTTTCCC TTGTCCAGAT AGCCCAGTAG CTGACATTCA TCCGGGGTCA

6481 GCACCGTTTC TGCGGACTGG CTTTCTACGT GTTCCGCTTC CTTTAGCAGC CCTTGCGCCC

6541 TGAGTGCTTG CGGCAGCGTG AAGCTAGCTT TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC

6601 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA

6661 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT

6721 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT

6781 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT

6841 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC

6901 ATTCCACAGC TGCCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT

6961 CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG

7021 CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CGCAGCCATG ACCCAGTCAC GTAGCGATAG

7081 CGGAGTGTAT ACTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCT

其他哺乳动物表达载体：

pEBVHis A	pcDNA5/TO	pDsRed2-Bid	pNFκB-MetLuc2-Reporter
pGL4.10	pBApo-CMV-neo	pAcGFP1-N1	pEF1α-IRES-DsRed-Express2
pGL4.29	pDsRed-Monomer	pSecTag2 A	pCMV-DsRed-Express2
pGL4.13	pIRES	pGL4.27	pcDNA3.1/NT-GFP-TOPO
pG5 luciferase	pIRES-hrGFP-1a	pGL4.26	pEF1α-IRES-ZsGreen1
pCMV-AD	pDsRed-Express2-N1	pACT	pCMV-Tag 2A
pRevTet-Off	pCMV-Tag 3B	pBIND-Id Control	pCMV-Tag 5B
pTet-Off	pCRE-hrGFP	pTRE2	pAcGFP1-C In-Fusion Ready
pTRE2-hygro	pDsRED2-Mito	pRevTRE	p3XFLAG-CMV-14
pVgRxR	pAcGFP1-F	pTK-hyg	p3XFLAG-CMV-8
pOPI3CAT	pAcGFP1-C1	pTRE3G-Luc	pFLAG-CMV-2
pBK-RSV	pAsRed2-C1	pSwitch	pcDNA3.3-TOPO
pIRES2-DsRed2	pAsRed2-N1	pcDNA4/His C	pcDNA6.2/cLumio-DEST
pCMV-Myc	pAcGFP1-Lam	c-Flag pcDNA3	pCMV-tdTomato
pCMV-Tag 2C	pAcGFP1-C	pcDNA4/TO/Myc-His A	pAcGFP1-Mito
pCMV-Tag 5A	pSEAP2-Basic	pcDNA6/myc-His B	pAcGFP1-N In-Fusion Ready
pCMV-Tag 3C	pBI-CMV3	pcDNA6/V5-His B	pDsRed-Monomer-N In-Fusion Ready
p3XFLAG-CMV-7	pNFkB-DD-tdTomato	pcDNA6.2/nTC-Tag-DEST	pcDNA4/TO/Myc-His B
p3XFLAG-CMV-9	pcDNA3.1/His A	pOptiVEC-TOPO	pIRES2-EGFP
pFLAG-CMV-4	pEBVHis B	pcDNA5/FRT	pcDNA3.1/His C
pBI-CMV4	pGL4.75	pGL4.30	pcDNA3.1/CT-GFP-TOPO
pcDNA4/His A	pGL4.20	pGL4.19	pEF1α-IRES-AcGFP1
pcDNA4/myc-His B	pCMV-SPORT6	pACT-MyoD	pcDNA3.2/V5/GW/D-TOPO
pcDNA4/HisMax C	pCMV-SPORT6	pCMV-BD	pcDNA4/TO/Myc-His/LacZ
pCMV-Tag 4A	pBIND	pCMV-Tet3G	pcDNA4/HisMax-TOPO
pcDNA6/myc-His C	pBD-NF-κB	pTet on advanced	p3XFLAG-CMV-13
pCMV-Tag 2B	pRevTet-On	pTRE-Tight	p3xFLAG-CMV-10
pGRN145	pTet-On	pIND	pFLAG-CMV-3
pCMV-MEK1	pTRE3G	pGene/V5-His B	pcDNA4/TO/Myc-His C
pCMV-Tag 3A	pcDNA4/TO	pOPRSVI	pcDNA6.2/C-YFP-DEST
pCMVLacI	pcDNA4/His B	pcDNA4/HisMax A	pcDNA6.2/cTC-Tag-DEST
pBI-CMV1	pcDNA4/HisMax B	pIRESpuro3	pcDNA6.2/nGeneBLAzer-DEST
pEF1α-AcGFP1-N1	pcDNA4/myc-His C	pIRESneo3	pCRE-MetLuc2-Reporter
pCMV-LacZ	pcDNA3.1/His B	pIRESneo2	pEF1α-DsRed-Express2
pCMV-Tag 4B	pcDNA6/V5-His C	pcDNA4/myc-His A	pDsRed-Express-C1
pCMV-Tag 5C	pCHO1.0	pCMV-PKA	pEF1α-DsRed-Monomer-N1
plRES2-ZsGreen1	pGL3-Promoter	pAcGFP1-N3	pDD-AmCyan1 Reporter
p3XFLAG-CMV-7.1	pCMV-MEKK1	pcDNA5/FRT/TO	pCRE-DD-AmCyan1
pFLAG-CMV-5a	pFLAG-CMV2	pBApo-CMV-Pur	pIRES2-DsRed-Express2
pBudCE4.1	pAcGFP1-C2	pBApo-EF1α-pur	pDsRed-Express-N1
pREP4	ptdTomato-C1	ptdTomato-N1	pcDNA6.2/nLumio-DEST
pBApo-CMV	pCRE-DD-tdTomato	pAcGFP1-Golgi	pcDNA6/myc-His A
pIRES2-AcGFP1	pAcGFP1-Hyg-C1	pAcGFP1-p53	pcDNA6/V5-His A
pIREShyg3	pAcGFP1-Mem	pAcGFP1-Actin	pcDNA5/FRT/TO-TOPO
pcDNA6/TR	pAcGFP1-C3	pBI-CMV2	pcDNA6.2/V5/GW/D-TOPO
pDsRed-Express2-C1	pTT5	pEF1α-tdTomato	pcDNA6.2/cGeneBLAzer-DEST
pBI-CMV5	pSEAP2-Control	pAmCyan1-C1	pcDNA6.2/nGeneBLAzer-GW/D-TOPO