pBI-CMV2

pBI-CMV2

pBI-CMV2载体基本信息

pBI-CMV2载体质粒图谱和多克隆位点信息

pBI-CMV2载体简介

双向启动子载体pBI-CMV2表达绿色荧光蛋白AcGFP1  

The pBI bidirectional vectors are designed to constitutively express a protein of interest and a reporter protein (or two proteins of interest). Expression is driven by constitutively active bidirectional human cytomegalovirus promoters (PminCMV). The gene(s) of interest must contain an initiation codon and a stop codon.  eatures Constitutively express a protein of interest and a reporter protein (or two proteins of interest)  pBI-CMV2 allows you to express a protein of interest and AcGFP1, a monomeric green fluorescent protein derived from Aequorea coerulescens.

pBI-CMV2载体序列

ORIGIN

    1 CTCGAGTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA

   61 GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG

  121 CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG

  181 ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA

  241 TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC

  301 CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC

  361 TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC

  421 ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA

  481 TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG

  541 GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA

  601 GGGATCCTCT AGTCAGCTGA CGCGTGCTAG CGCGGCCGCA TCGATAAGCT TGTCGACGAT

  661 ATCTCCAGAG GATCATAATC AGCCATACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA

  721 ACCTCCCACA CCTCCCCCTG AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTTAACT

  781 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA

  841 AAGCATTTTT TTCACTGCCC CGAGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC

  901 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG

  961 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA

 1021 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC

 1081 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC

 1141 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG

 1201 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT

 1261 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC

 1321 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC

 1381 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG

 1441 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG

 1501 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA

 1561 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG

 1621 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT

 1681 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA

 1741 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT

 1801 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG

 1861 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA

 1921 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC

 1981 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT

 2041 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG

 2101 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA

 2161 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG

 2221 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA

 2281 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG

 2341 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT

 2401 CTTGCCCGGC GTCAACACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA

 2461 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA

 2521 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG

 2581 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC

 2641 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT

 2701 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC

 2761 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCGGCAG TGAAAAAAAT GCTTTATTTG

 2821 TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA

 2881 CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA

 2941 AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT GATCCTCTAG ACTGCAGCCT

 3001 CAGGAGATCT GGGCCCTCAC TTGTACAGCT CATCCATGCC GTGGGTGATG GCGGCGGCGG

 3061 TCACGAAGCC GAAGTAGATC ATGTGATCGC GCTTCTCGTT GGGGTCCTTG GACAGGGCGC

 3121 TCTGGGTGGA CAGGTAGTGG TTATCGGGCA GCAGCACAGG GCCATCGCCG ATGGGGGTAT

 3181 TCTGCTGGTA GTGGTCGGCC AGCTGCACGC TGCCATCCTC GATGTTGTGG CGGATCTTGA

 3241 AGTTCACCTT GATGCCATTC TTGGCCTTGT CGGTCATGAT GTACACATTG TGGGCGTTGT

 3301 AGTTGTACTC CATCTTATTG CCCAGGATGT TGCCATCCTC CTTGAAATCG GTGCCGGTCA

 3361 GCTCGATGCG ATTCACCAGG GTATCGCCCT CGAACTTCAC CTCGGCGCGC GACTTGTAGT

 3421 TGCCGTCATC CTCGAAGAAG ATGGTGCGCT CCTGGATGTA GCCCTCAGGC ATGGCGCTCT

 3481 TGAAGAAGTC GTGCTGCTTC ATGTGATCGG GGTAGCGTGA GAAGCACTGC ACGCCGTAGC

 3541 TCAGGGTGGT CACCAGGGTG GGCCAGGGCA CAGGCAGCTT GCCGGTGGTG CAGATGAACT

 3601 TCAGGGTCAG CTTGCCGTAG GTGGCATCGC CCTCGCCCTC GCCGCTCACG CTGAACTTGT

 3661 GGCCATTCAC ATCGCCATTC AGCTCGATCA GGATGGGCAC GATGCCGGTG AACAGCTCGG

 3721 CGCCCTTGCT CACCATGGTG GCGAATTCTC CAGGCGATCT GACGGTTCAC TAAACGAGCT

 3781 CTGCTTATAT AGGCCTCCCA CCGTACACGC CACCTCGACA TA

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