pLpp-OmpA
pLpp-OmpA
编号 | 载体名称 |
北京华越洋VECT90054 | pLpp-OmpA |
pLpp-OmpA pET系列表达载体基本信息：
启动子: | lpp/lac |
复制子: | ColE1 ori |
质粒分类: | 大肠杆菌载体；PET系列表达质粒 |
质粒大小: | 7533bp |
质粒标签: | N-OmpA，C-6×His |
原核抗性: | 氨苄青霉素Amp |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧，LB |
表达宿主: | BL21(DE3) |
诱导方式: | IPTG或乳糖及其类似物 |
5'测序引物: | 5 GGC TTT ACA CTT TAT GCT TC 3 |
3'测序引物: | 5CCA TTT TTC ACT TCA CAG G 3 |
pLpp-OmpA载体质粒图谱和多克隆位点信息：
pLpp-OmpA载体简介
pLpp-OmpA载体序列：
ORIGIN
1 GGATAACCAG AAGCAATAAA AAATCAAATC GGATTTCACT ATATAATCTC ACTTTATCTA
61 AGATGAATCC GATGGAAGCA TCCTGTTTTC TCTCAATTTT TTTATCTAAA ACCCAGCGTT
121 CGATGCTTCT TTGAGCGAAC GATCAAAAAT AAGTGCCTTC CCATCAAAAA AATATTCTCA
181 ACATAAAAAA CTTTGTGTAA TACTTGTAAC GCTACATGGA GATTAACTCA ATCTAGCTAG
241 AGAGGCTTTA CACTTTATGC TTCCGGCTCG TATAATGTGT GGAATTGTGA GCGGATAACA
301 ATTTCACACA GGAAACAGCT ATGACCATGA TTACGGATTC ACTGGAACTC TAGATAACGA
361 GGCGCAAAAA ATGAAAAAGA CAGCTATCGC GATTGCAGTG GCACTGGCAG GTTTCGCTAC
421 CGTCGCTCAG GCTGGATCCG CAGAATTCAG CGCTAGCCAC CATCATCATC ATCATTAAGC
481 TTAGATCCGG CTGAGCAACG ACGTGAACGC AATGCGTTCC GACGTTCAGG CTGCTAAAGA
541 TGACGCAGCT CGTGCTAACC AGCGTCTGGA CAACATGGCT ACTAAATACC GCAAGTAATA
601 GTACCTGTGA AGTGAAAAAT GGCGCACATT GTGCGACATT TTTTTTGTCT GCCGTTTACC
661 GCTACTGCGT CACGCGTAAC ATATTCCCTT GCTCTGGTTC ACCATTCTGC GCTGACTCTA
721 CTGAAGGCGC ATTGCTGGCT GCGGGAGTTG CTCCACTGCT CACCGAAACC GGATACCCTG
781 CCCGACGATA CAACGCTTTA TCGACTAACT TCTGATCTAC AGCCTTATTG TCTTTAAATT
841 GCGTAAAGCC TGCTGGCAGT GTGTATGGCA TTGTCTGAAC GTTCTGCTGT TCTTCTGCCG
901 ATAGTGGTCG ATGTACTTCA ACATAACGCA TCCCGTTAGG CTCCACGGAA TATTTCACCG
961 GTTCGTTGAT CACTTTCACC GGCGTTCCCG TCCGCACGCT GGAGAACAAG GCTTTAATAT
1021 CCGGTGCATT CATGCGAATA CACCCTGAAC TGACGCGCAA ACCGACGCTG TCCGGCGCAC
1081 TGGTACCATG AATGAGGTAT TCGCCATTAC CATGCGCGAG GCGCAGTGCG TAACGTCCTA
1141 GCGGGTTATT TGGTCCGGCA GGAACGACTG GCGGTAATTT AATGCCACGC TCCAGCGAAC
1201 GCTGACGAAT GCCTGCCGTA GGCGTCCAGG TTGGGTTAGG GATTTTCTGC CCAACACGCG
1261 TTTCCATCAC CGGCGTTTCC AGCCCCTGCA ATCCAATACC TATTGGATAA ACCTGCACAA
1321 TATTTTCTCC CGGCGGATAA TAATAAAGGC GCAGCTCTGC AAGGTTGGTC GACGGTCGAC
1381 CGGTCTCACT TCAACATAAC GCATCCCGTT AGGCTCCACG GAATATTTCA CCGGTTCGTT
1441 GATCACTTTC ACCGGCGTTC CCGTCCGCAC GCTGGAGAAC AAGGCTTTAA TATCCGGTGC
1501 ATTCATGCGA ATACACCCTG AACTGACGCG CAAACCGACG CTGTCCGGCG CACTGGTACC
1561 ATGAATGAGG TATTCGCCAT TACCATGCGC GAGGCGCAGT GCGTAACGTC CTAGCGGGTT
1621 ATTTGGTCCG GCAGGAACGA CTGGCGGTAA TTTAATGCCA CGCTCCAGCG AACGCTGACG
1681 AATGCCTGCC GTAGGCGTCC AGGTTGGGTT AGGGATTTTC TGCCCAACAC GCGTTTCCAT
1741 CACCGGCGTT TCCAGCCCCT GCAATCCAAT ACCTATTGGA TAAACCTGCA CAATATTTTC
1801 TCCCGGCGGA TAATAATAAA GGCGCAGCTC TGCAAGGTTG ACACCATCGA ATGGCGCAAA
1861 ACCTTTCGCG GTATGGCATG ATAGCGCCCG GAAGAGAGTC AATTCAGGGT GGTGAATGTG
1921 AAACCAGTAA CGTTATACGA TGTCGCAGAG TATGCCGGTG TCTCTTATCA GACCGTTTCC
1981 CGCGTGGTGA ACCAGGCCAG CCACGTTTCT GCGAAAACGC GGGAAAAAGT GGAAGCGGCG
2041 ATGGCGGAGC TGAATTACAT TCCCAACCGC GTGGCACAAC AACTGGCGGG CAAACAGTCG
2101 TTGCTGATTG GCGTTGCCAC CTCCAGTCTG GCCCTGCACG CGCCGTCGCA AATTGTCGCG
2161 GCGATTAAAT CTCGCGCCGA TCAACTGGGT GCCAGCGTGG TGGTGTCGAT GGTAGAACGA
2221 AGCGGCGTCG AAGCCTGTAA AGCGGCGGTG CACAATCTTC TCGCGCAACG CGTCAGTGGG
2281 CTGATCATTA ACTATCCGCT GGATGACCAG GATGCCATTG CTGTGGAAGC TGCCTGCACT
2341 AATGTTCCGG CGTTATTTCT TGATGTCTCT GACCAGACAC CCATCAACAG TATTATTTTC
2401 TCCCATGAAG ACGGTACGCG ACTGGGCGTG GAGCATCTGG TCGCATTGGG TCACCAGCAA
2461 ATCGCGCTGT TAGCGGGCCC ATTAAGTTCT GTCTCGGCGC GTCTGCGTCT GGCTGGCTGG
2521 CATAAATATC TCACTCGCAA TCAAATTCAG CCGATAGCGG AACGGGAAGG CGACTGGAGT
2581 GCCATGTCCG GTTTTCAACA AACCATGCAA ATGCTGAATG AGGGCATCGT TCCCACTGCG
2641 ATGCTGGTTG CCAACGATCA GATGGCGCTG GGCGCAATGC GCGCCATTAC CGAGTCCGGG
2701 CTGCGCGTTG GTGCGGATAT CTCGGTAGTG GGATACGACG ATACCGAAGA CAGCTCATGT
2761 TATATCCCGC CGTTAACCAC CATCAAACAG GATTTTCGCC TGCTGGGGCA AACCAGCGTG
2821 GACCGCTTGC TGCAACTCTC TCAGGGCCAG GCGGTGAAGG GCAATCAGCT GTTGCCCGTC
2881 TCACTGGTGA AAAGAAAAAC CACCCTGGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
2941 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA
3001 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT
3061 GCTAACGATA ATCCCTGACG CGGTGCATCA GGTAATAACA GTTGTGAAGG AATAGTTATC
3121 GTCGTACCAG GTTTTGGCAC CGGGGCGATA GTGTTATTGG CTTCAAGGAT CAACATTGCC
3181 GCAGTATCAA AACGTCGGGC AATAGCCTGA AGGTTTTTAT CCCCTTCTTG CACCGTATAC
3241 GTTTGATTTT GCCCAACCAG TCGGCTTCCG GTTGGTGGTA GCGGATAATC AACCGCCCAG
3301 GCAGCCTGGA TGGCGCTAAA AGCGCCGATA AGCGTGAGTG TAAGCAAAGA CGCGCGTTTC
3361 ATTGTAAACC TCCTGTATTT GCCGGAGACT CACGCTGAAA CGTCGGATGG CGCTTATGTT
3421 CACCTGAAAC CAAAACACTC CTGTGCAGGT CAGTGTAAAC ATTGACCATC CGGCAATGTG
3481 AGCCAACCGG ATGAAAGCTG TCCTTTTAGT TTAGCTAAGT GCAGCGGCTT TGGCGCGAAT
3541 TGCGCGAATC ATCGCTTCCA GACCTTGTGA ACGAGATGGG GTGAGATGTT GGGTGAGCGC
3601 CATTTTTTCA AACCACGGAC GCACATCGAA ATTGACAATA TCCTGCGGCG TCATCTGATC
3661 GTAGAGAATA AAGACGACCG CAATAAGCCC TTTCACAATC GCCGCATCGC TGTCGCCCTG
3721 TAATTCAATA ATTCCCTGGG CATTCTGGCG CATGACAATC CACACCTGAC TCTGACAGCC
3781 CTGAATGCTA TTTTGTGGAC TTCTGTCTTC GTCGCGTAAT TCTGGCAGAC GCTGGGGGAC
3841 CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG GGCATGACTA
3901 TCGTCGCCGC ACTTATGACT GTCTTCTTTA TCATGCAACT CGTAGGACAG GTGCCGGCAG
3961 CGCTCTGGGT CATTTTCGGC GAGGACCGCT TTCGCTGGAG CGCGACGATG ATCGGCCTGT
4021 CGCTTGCGGT ATTCGGAATC TTGCACGCCC TCGCTCAAGC CTTCGTCACT GGTCCCGCCA
4081 CCAAACGTTT CGGCGAGAAG CAGGCCATTA TCGCCGGCAT GGCGGCCGAC GCGCTGGGCT
4141 ACGTCTTGCT GGCGTTCGCG ACGCGAGGCT GGATGGCCTT CCCCATTATG ATTCTTCTCG
4201 CTTCCGGCGG CATCGGGATG CCCGCGTTGC AGGCCATGCT GTCCAGGCAG GTAGATGACG
4261 ACCATCAGGG ACAGCTTCAA GGATCGCTCG CGGCTCTTAC CAGCCTAACT TCGATCACTG
4321 GACCGCTGAT CGTCACGGCG ATTTATGCCG CCTCGGCGAG CACATGGAAC GGGTTGGCAT
4381 GGATTGTAGG CGCCGCCCTA TACCTTGTCT GCCTCCCCGC GTTGCGTCGC GGTGCATGGA
4441 GCCGGGCCAC CTCGACCTGA ATGGAAGCCG GCGGCACCTC GCTAACGGAT TCACCACTCC
4501 AAGAATTGGA GCCAATCAAT TCTTGCGGAG AACTGTGAAT GCGCAAACCA ACCCTTGGCA
4561 GAACATATCC ATCGCGTCCG CCATCTCCAG CAGCCGCACG CGGCGCATCT CGGGCAGCGT
4621 TGGGTCCTGG CCACGGGTGC GCATGATCGT GCTCCTGTCG TTGAGGACCC GGCTAGGCTG
4681 GCGGGGTTGC CTTACTGGTT AGCAGAATGA ATCACCGATA CGCGAGCGAA CGTGAAGCGA
4741 CTGCTGCTGC AAAACGTCTG CGACCTGAGC AACAACATGA ATGGTCTTCG GTTTCCGTGT
4801 TTCGTAAAGT CTGGAAACGC GGAAGTCAGC GCCCTGCACC ATTATGTTCC GGATCTGCAT
4861 CGCAGGATGC TGCTGGCTAC CCTGTGGAAC ACCTACATCT GTATTAACGA AGCGCTGGCA
4921 TTGACCCTGA GTGATTTTTC TCTGGTCCCG CCGCATCCAT ACCGCCAGTT GTTTACCCTC
4981 ACAACGTTCC AGTAACCGGG CATGTTCATC ATCAGTAACC CGTATCGTGA GCATCCTCTC
5041 TCGTTTCATC GGTATCATTA CCCCCATGAA CAGAAATCCC CCTTACACGG AGGCATCAGT
5101 GACCAAACAG GAAAAAACCG CCCTTAACAT GGCCCGCTTT ATCAGAAGCC AGACATTAAC
5161 GCTTCTGGAG AAACTCAACG AGCTGGACGC GGATGAACAG GCAGACATCT GTGAATCGCT
5221 TCACGACCAC GCTGATGAGC TTTACCGCAG CTGCCTCGCG CGTTTCGGTG ATGACGGTGA
5281 AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG
5341 GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT
5401 GACCCAGTCA CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC ATCAGAGCAG
5461 ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA
5521 TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG
5581 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
5641 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG
5701 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
5761 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
5821 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
5881 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
5941 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC
6001 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
6061 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
6121 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT
6181 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
6241 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA
6301 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
6361 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
6421 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
6481 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
6541 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
6601 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
6661 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
6721 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
6781 GTTGCCATTG CTGCAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
6841 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
6901 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
6961 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
7021 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
7081 TGCCCGGCGT CAACACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
7141 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
7201 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
7261 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
7321 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
7381 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
7441 CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA
7501 ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTT
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pLpp-OmpA其他相关pET系列表达载体：
