北京华越洋生物
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首页    载体及质粒    pQE-T7-1
pQE-T7-1

pQE-T7-1

pQE-T7-1

pQE-T7-1

 



编号

载体名称

北京华越洋VECT90075

pQE-T7-1

 

pQE-T7-1  pQE系列表达载体基本信息:

启动子:

T7

复制子:

ColE1 oriF1 ori

终止子:

T7 terminator

质粒分类:

大肠杆菌载体;pQE系列表达质粒

质粒大小:

5317bp

原核抗性:

卡那霉素Kan

克隆菌株:

DH5α

培养条件:

37,有氧,LB

表达宿主:

M15

诱导方式:

IPTG或乳糖及其类似物

 

pQE-T7-1载体质粒图谱和多克隆位点信息:

 

pQE-T7-1载体简介

pQE-T7-1载体序列:

ORIGIN
        1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
       61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
      121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTCTTACT AGGCTGCAGA
      181 CGTGGGTACC CGCGGCCGCA TGAGCTCCAT GCATATGATG ATGGTGGTGA TGGTGATGAT
      241 GATGTTTCAT ATTTATATCT CCTTCTTAAA GTTAAACAAA ATTATTTCTA GAGGGGAATT
      301 GTTATCCGCT CACAATTCCC CTATAGTGAG TCGTATTAAT TTCGCGGGAT CGAGATCTCG
      361 ATCCTCTACG CCGGACGCAT CGTGGCCGGC ATCACCGGCG CCACAGGTGC GGTTGCTGGC
      421 GCCTATATCG CCGACATCAC CGATGGGGAA GATCGGGCTC GCCACTTCGG GCTCATGAGC
      481 GCTTGTTTCG GCGTGGGTAT GGTGGCAGGC CCCGTGGCCG GGGGACTGTT GGGCGCCATC
      541 TCCTTGCATG CACCATTCCT TGCGGCGGCG GTGCTCAACG GCCTCAACCT ACTACTGGGC
      601 TGCTTCCTAA TGCAGGAGTC GCATAAGGGA GAGCGTCGAG ATCCCGGACA CCATCGAATG
      661 GCGCAAAACC TTTCGCGGTA TGGCATGATA GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT
      721 GAATGTGAAA CCAGTAACGT TATACGATGT CGCAGAGTAT GCCGGTGTCT CTTATCAGAC
      781 CGTTTCCCGC GTGGTGAACC AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA
      841 AGCGGCGATG GCGGAGCTGA ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA
      901 ACAGTCGTTG CTGATTGGCG TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT
      961 TGTCGCGGCG ATTAAATCTC GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT
     1021 AGAACGAAGC GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT
     1081 CAGTGGGCTG ATCATTAACT ATCCGCTGGA TGACCAGGAT GCCATTGCTG TGGAAGCTGC
     1141 CTGCACTAAT GTTCCGGCGT TATTTCTTGA TGTCTCTGAC CAGACACCCA TCAACAGTAT
     1201 TATTTTCTCC CATGAAGACG GTACGCGACT GGGCGTGGAG CATCTGGTCG CATTGGGTCA
     1261 CCAGCAAATC GCGCTGTTAG CGGGCCCATT AAGTTCTGTC TCGGCGCGTC TGCGTCTGGC
     1321 TGGCTGGCAT AAATATCTCA CTCGCAATCA AATTCAGCCG ATAGCGGAAC GGGAAGGCGA
     1381 CTGGAGTGCC ATGTCCGGTT TTCAACAAAC CATGCAAATG CTGAATGAGG GCATCGTTCC
     1441 CACTGCGATG CTGGTTGCCA ACGATCAGAT GGCGCTGGGC GCAATGCGCG CCATTACCGA
     1501 GTCCGGGCTG CGCGTTGGTG CGGATATCTC GGTAGTGGGA TACGACGATA CCGAAGACAG
     1561 CTCATGTTAT ATCCCGCCGT TAACCACCAT CAAACAGGAT TTTCGCCTGC TGGGGCAAAC
     1621 CAGCGTGGAC CGCTTGCTGC AACTCTCTCA GGGCCAGGCG GTGAAGGGCA ATCAGCTGTT
     1681 GCCCGTCTCA CTGGTGAAAA GAAAAACCAC CCTGGCGCCC AATACGCAAA CCGCCTCTCC
     1741 CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG
     1801 GCAGTGAGCG CAACGCAATT AATGTAAGTT AGCTCACTCA TTAGGCACCG GGATCTCGAC
     1861 CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG GGCATGACTA
     1921 TCGTCGCCGC ACTTATGACT GTCTTCTTTA TCATGCAACT CGTAGGACAG GTGCCGGCAG
     1981 CGCTCTGGGT CATTTTCGGC GAGGACCGCT TTCGCTGGAG CGCGACGATG ATCGGCCTGT
     2041 CGCTTGCGGT ATTCGGAATC TTGCACGCCC TCGCTCAAGC CTTCGTCACT GGTCCCGCCA
     2101 CCAAACGTTT CGGCGAGAAG CAGGCCATTA TCGCCGGCAT GGCGGCCCCA CGGGTGCGCA
     2161 TGATCGTGCT CCTGTCGTTG AGGACCCGGC TAGGCTGGCG GGGTTGCCTT ACTGGTTAGC
     2221 AGAATGAATC ACCGATACGC GAGCGAACGT GAAGCGACTG CTGCTGCAAA ACGTCTGCGA
     2281 CCTGAGCAAC AACATGAATG GTCTTCGGTT TCCGTGTTTC GTAAAGTCTG GAAACGCGGA
     2341 AGTCAGCGCC CTGCACCATT ATGTTCCGGA TCTGCATCGC AGGATGCTGC TGGCTACCCT
     2401 GTGGAACACC TACATCTGTA TTAACGAAGC GCTGGCATTG ACCCTGAGTG ATTTTTCTCT
     2461 GGTCCCGCCG CATCCATACC GCCAGTTGTT TACCCTCACA ACGTTCCAGT AACCGGGCAT
     2521 GTTCATCATC AGTAACCCGT ATCGTGAGCA TCCTCTCTCG TTTCATCGGT ATCATTACCC
     2581 CCATGAACAG AAATCCCCCT TACACGGAGG CATCAGTGAC CAAACAGGAA AAAACCGCCC
     2641 TTAACATGGC CCGCTTTATC AGAAGCCAGA CATTAACGCT TCTGGAGAAA CTCAACGAGC
     2701 TGGACGCGGA TGAACAGGCA GACATCTGTG AATCGCTTCA CGACCACGCT GATGAGCTTT
     2761 ACCGCAGCTG CCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC
     2821 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG
     2881 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC CCAGTCACGT AGCGATAGCG
     2941 GAGTGTATAC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATTG
     3001 CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG CTCTTCCGCT
     3061 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC
     3121 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA
     3181 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT
     3241 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC
     3301 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT
     3361 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG
     3421 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG
     3481 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT
     3541 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG
     3601 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC
     3661 GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA
     3721 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT
     3781 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT
     3841 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAAC
     3901 AATAAAACTG TCTGCTTACA TAAACAGTAA TACAAGGGGT GTTATGAGCC ATATTCAACG
     3961 GGAAACGTCT TGCTCTAGGC CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA
     4021 TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA ATCTATCGAT TGTATGGGAA
     4081 GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC
     4141 AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA
     4201 TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT GCGATCCCCG GGAAAACAGC
     4261 ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT
     4321 GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT
     4381 ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT
     4441 TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT
     4501 GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT
     4561 TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA
     4621 CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG
     4681 GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT
     4741 GCTCGATGAG TTTTTCTAAG AATTAATTCA TGAGCGGATA CATATTTGAA TGTATTTAGA
     4801 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG
     4861 CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA
     4921 ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG
     4981 TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG
     5041 GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT
     5101 TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG
     5161 AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC
     5221 GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC
     5281 GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCA
//

 

pQE-T7-1其他相关pQE系列表达载体:

pETBlue-2

pQE-30   UA   (linearized)

pET30a-EcoRV

pQE-22

pET28a-SUMO

pQE-19

pET28a-OFP

pQE-18

pET28a-mCherry

pQE-17

pET28a-EYFP

pQE-15

pET28a-EGFP

pQE-14

pET28a-ECFP

pQE-13

pET28a-EBFP

pQE-12

pET28a-DsRed2

pQE-11

pTrc-CKS

pQE-10

pMBP-P

pQE-8

pMBP-C

pQE-7

pLpp-OmpA

pQE-6

pLLP-STII/Plpp-STII

pQE-5

pET-Trx

pQE-4

pETM-30

pQE-3

pETM-11

pCold-SUMO-His-FLAG

pET-GST

pCold-SUMO-His-FLAG

pET-DsbA

pCold-SUMO

pET22b-EGFP

pCold-NusA

TAGZyme     pQE-2

pCold-MBP

TAGZyme     pQE-1

pCold-DsbA

pQE-TriSystem-7

pUC57-Kan

pQE-TriSystem-6

pUC57-Simple

pQE-TriSystem-5

pUC57

pQE-TriSystem     Strep

pUC119

pQE-TriSystem     His-Strep2

pUC118

pQE-TriSystem     His-Strep1

pJET1.2   T   Recombined

pQE-T7-2

pMD-19TSimple

pQE-T7-1

pGEM-3Zf(+)

pQE-T7(cloning     region)

pBluescript     II SK(+)

pQE-100     DoubleTaq

pBluescript     II KS(+)

pQE-82L-Kan

pUC19

pQE-81L-Kan

pUC18

pQE-80L-Kan

pMD-18T

pQE-80

pUC57-Tac-mCherry

pQE-52

pTYB21

pQE-51

pTYB4

pQE-50

pTrcHisC

pQE-42

pTrcHisB

pQE-40

pTrcHisA

pQE-30   Xa

pTrcHis2C

pQE-30   UA

pTrcHis2B

pTrcHis2A

pBAD/Myc-HisA

pRSETC

pBAD/His     /LacZ

pRSETB

pBAD/His-C

pMCSG19

pBAD/His-B

pMCSG9

pBAD/His-A

pLAFR-3

pBAD30

pEGFP-Lac

pGEX-Zsgreen1

pDrive

pGEX-TagRFP

pBV222

pGEX-His

pBAD/Myc-HisC

pGEX-20T

pBAD/Myc-HisB

pGEX-1λT     EcoRI/BAP