pQE-TriSystem Strep
pQE-TriSystem Strep
编号 | 载体名称 |
北京华越洋VECT90071 | pQE-TriSystem Strep |
pQE-TriSystem Strep pQE系列表达载体基本信息：
启动子: | p10 |
复制子: | ColE1 ori |
终止子: | T7 terminator |
质粒分类: | 大肠杆菌载体；pQE系列表达质粒 |
质粒大小: | 5736bp |
原核抗性: | 氨苄青霉素Amp |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧，LB |
表达宿主: | M15 |
诱导方式: | IPTG或乳糖及其类似物 |
pQE-TriSystem Strep载体质粒图谱和多克隆位点信息：
pQE-TriSystem Strep载体简介
pQE-TriSystem Strep载体序列：
ORIGIN
1 CTCTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT
61 CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC
121 ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG
181 TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT
241 GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA
301 GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT
361 TACCATGCAT GGTCGAGGTG AGCCCCACGT TCTGCTTCAC TCTCCCCATC TCCCCCCCCT
421 CCCCACCCCC AATTTTGTAT TTATTTATTT TTTAATTATT TTGTGCAGCG ATGGGGGCGG
481 GGGGGGGGGG GGGGCGCGCG CCAGGCGGGG CGGGGCGGGG CGAGGGGCGG GGCGGGGCGA
541 GGCGGAGAGG TGCGGCGGCA GCCAATCAGA GCGGCGCGCT CCGAAAGTTT CCTTTTATGG
601 CGAGGCGGCG GCGGCGGCGG CCCTATAAAA AGCGAAGCGC GCGGCGGGCG GGAGTCGTGC
661 GCGCTGCCTT CGCCCCGTGC CCCGCTCCGC CGCCGCCTCG CGCCGCCCGC CCCGGCTCTG
721 ACTGACCGCG TTACTCCCAC AGGTGAGCGG GCGGGACGGC CCTTCTCCTT CGGGCTGTAA
781 TTAGCGCTTG GTTTAATGAC GGCTTGTTTC TTTTCTGTGG CTGCGTGAAA GCCTTGAGGG
841 GCTCCGGGAG GGCCCTTTGT GCGGGGGGAG CGGCTCGGGG CTGTCCGCGG GGGGACGGCT
901 GCCTTCGGGG GGGACGGGGC AGGGCGGGGT TCGGCTTCTG GCGTGTGACC GGCGGCTCTA
961 GAGCCTCTGC TAACCATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC
1021 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGGA TCGGACCGGA AATCATAAAA
1081 AATTATTTGC TTTGTGAGCG GATAACAATT ATAATAGATT CAATTGAGGC CTCGACCACC
1141 GGGACCTTTA ATTCAACCCA ACACAATATA TTATAGTTAA ATAAGAATTA TTATCAAATC
1201 ATTTGTATAT TAATTAAAAT ACTATACTGT AAATTACATT TTATTTACAA TCAAAGGAGA
1261 TATACCATGG CGATATCCCG GGAGCTCGTG GATCCGAATT CTCAGATCTC GGCGCGCCTG
1321 CAGGTCGACG GTACCGGTTC GAAGCTTGCG GCCGCACAGC TGTATACACG TGCAAGCGCG
1381 TGGAGCCACC CGCAGTTCGA AAAATAACAC TAAGTGATTA ACCTCAGGTG CAGGCTGCCT
1441 ATCAGAAGGT GGTGGCTGGT GTGGCCAATG CCCTGGCTCA CAAATACCAC TGAGATCGAT
1501 CTTTTTCCCT CTGCCAAAAA TTATGGGGAC ATCATGAAGC CCCTTGAGCA TCTGACTTCT
1561 GGCTAATAAA GGAAATTTAT TTTCATTGCA ATAGTGTGTT GGAATTTTTT GTGTCTCTCA
1621 CTCGGAAGGA CATATGGGAG GGCAAATCAT TTAAAACATC AGAATGAGTT TTTGGTTTAG
1681 AGTTTGGCAA CATATGCCCA TATGTAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
1741 TTGAGGGGTT TTTTGCTGAA AGCATGCGGA GGAAATTCTC CTTGAAGTTT CCCTGGTGTT
1801 CAAAGTAAAG GAGTTTGCAC CAGACGCACC TCTGTTCACT GGTCCGGCGT ATTAAAACAC
1861 GATACATTGT TATTAGTACA TTTATTAAGC GCTAGATTCT GTGCGTTGTT GATTTACAGA
1921 CAATTGTTGT ACGTATTTTA ATAATTCATT AAATTTATAA TCTTTAGGGT GGTATGTTAG
1981 AGCGAAAATC AAATGATTTT CAGCGTCTTT ATATCTGAAT TTAAATATTA AATCCTCAAT
2041 AGATTTGTAA AATAGGTTTC GATTAGTTTC AAACAAGGGT TGTTTTTCCG AACCGATGGC
2101 TGGACTATCT AATGGATTTT CGCTCAACGC CACAAAACTT GCCAAATCTT GTAGCAGCAA
2161 TCTAGCTTTG TCGATATTCG TTTGTGTTTT GTTTTGTAAT AAAGGTTCGA CGTCGTTCAA
2221 AATATTATGC GCTTTTGTAT TTCTTTCATC ACTGTCGTTA GTGTACAATT GACTCGACGT
2281 AAACACGTTA AATAGAGCTT GGACATATTT AACATCGGGC GTGTTAGCTT TATTAGGCCG
2341 ATTATCGTCG TCGTCCCAAC CCTCGTCGTT AGAAGTTGCT TCCGAAGACG ATTTTGCCAT
2401 AGCCACACGA CGCCTATTAA TTGTGTCGGC TAACACGTCC GCGATCAAAT TTGTAGTTGA
2461 GCTTTTTGGA ATTATTTCTG ATTGCGGGCG TTTTTGGGCG GGTTTCAATC TAACTGTGCC
2521 CGATTTTAAT TCAGACAACA CGTTAGAAAG CGATGGTGCA GGCGGTGGTA ACATTTCAGA
2581 CGGCAAATCT ACTAATGGCG GCGGTGGTGG AGCTGATGAT AAATCTACCA TCGGTGGAGG
2641 CGCAGGCGGG GCTGGCGGCG GAGGCGGAGG CGGAGGTGGT GGCGGTGATG CAGACGGCGG
2701 TTTAGGCTCA AATGTCTCTT TAGGCAACAC AGTCGGCACC TCAACTATTG TACTGGTTTC
2761 GGGCGCCGTT TTTGGTTTGA CCGGTCTGAG ACGAGTGCGA TTTTTTTCGT TTCTAATAGC
2821 TTCCAACAAT TGTTGTCTGT CGTCTAAAGG TGCAGCGGGT TGAGGTTCCG TCGGCATTGG
2881 TGGAGCGGGC GGCAATTCAG ACATCGATGG TGGTGGTGGT GGTGGAGGCG CTGGAATGTT
2941 AGGCACGGGA GAAGGTGGTG GCGGCGGTGC CGCCGGTATA ATTTGTTCTG GTTTAGTTTG
3001 TTCGCGCACG ATTGTGGGCA CCGGCGCAGG CGCCGCTGGC TGCACAACGG AAGGTCGTCT
3061 GCTTCGAGGC AGCGCTTGGG GTGGTGGCAA TTCAATATTA TAATTGGAAT ACAAATCGTA
3121 AAAATCTGCT ATAAGCATTG TAATTTCGCT ATCGTTTACC GTGCCGATAT TTAACAACCG
3181 CTCAATGTAA GCAATTGTAT TGTAAAGAGA TTGTCTCAAG CTCGGAACGC TGCGCTCGGT
3241 CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA
3301 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG
3361 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCTGACGA GCATCACAAA
3421 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT
3481 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG
3541 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC
3601 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC
3661 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA
3721 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
3781 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC
3841 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA
3901 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA
3961 AAAGGATCTC AAGAAGATCC TTTGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
4021 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT
4081 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC
4141 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC
4201 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG
4261 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG
4321 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG
4381 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG
4441 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT
4501 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA
4561 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC
4621 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC
4681 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC
4741 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC
4801 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA
4861 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGTCC GCGCGTTTCC TGCATCTTTT
4921 AATCAAATCC CAAGATGTGT ATAAACCACC AAACTGCCAA AAAATGAAAA CTGTCGACAA
4981 GCTCTGTCCG TTTGCTGGCA ACTGCAAGGG TCTCAATCCT ATTTGTAATT ATTGAATAAT
5041 AAAACAATTA TAAATGTCAA ATTTGTTTTT TATTAACGAT ACAAACCAAA CGCAACAAGA
5101 ACATTTGTAG TATTATCTAT AATTGAAAAC GCGTAGTTAT AATCGCTGAG GTAATATTTA
5161 AAATCATTTT CAAATGATTC ACAGTTAATT TGCGACAATA TAATTTTATT TTCACATAAA
5221 CTAGACGCCT TGTCGTCTTC TTCTTCGTAT TCCTTCTCTT TTTCATTTTT CTCTTCATAA
5281 AAATTAACAT AGTTATTATC GTATCCATAT ATGTATCTAT CGTATAGAGT AAATTTTTTG
5341 TTGTCATAAA TATATATGTC TTTTTTAATG GGGTGTATAG TACCGCTGCG CATAGTTTTT
5401 CTGTAATTTA CAACAGTGCT ATTTTCTGGT AGTTCTTCGG AGTGTGTTGC TTTAATTATT
5461 AAATTTATAT AATCAATGAA TTTGGGATCG TCGGTTTTGT ACAATATGTT GCCGGCATAG
5521 TACGCAGCTT CTTCTAGTTC AATTACACCA TTTTTTAGCA GCACCGGATT AACATAACTT
5581 TCCAAAATGT TGTACGAACC GTTAAACAAA AACAGTTCAC CTCCCTTTTC TATACTATTG
5641 TCTGCGAGCA GTTGTTTGTT GTTAAAAATA ACAGCCATTG TAATGAGACG CACAAACTAA
5701 TATCACAAAC TGGAAATGTC TATCAATATA TAGTTG
//
pQE-TriSystem Strep其他相关pQE系列表达载体：
