pQE-TriSystem His-Strep2
pQE-TriSystem His-Strep2
编号 | 载体名称 |
北京华越洋VECT90072 | pQE-TriSystem His-Strep2 |
pQE-TriSystem His-Strep2 pQE系列表达载体基本信息：
启动子: | p10 |
复制子: | ColE1 ori |
终止子: | T7 terminator |
质粒分类: | 大肠杆菌载体；pQE系列表达质粒 |
质粒大小: | 5796bp |
原核抗性: | 氨苄青霉素Amp |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧，LB |
表达宿主: | M15 |
诱导方式: | IPTG或乳糖及其类似物 |
pQE-TriSystem His-Strep2载体质粒图谱和多克隆位点信息：
pQE-TriSystem His-Strep2载体简介
pQE-TriSystem His-Strep2载体序列：
ORIGIN
1 CTCTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT
61 CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC
121 ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG
181 TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT
241 GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA
301 GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT
361 TACCATGCAT GGTCGAGGTG AGCCCCACGT TCTGCTTCAC TCTCCCCATC TCCCCCCCCT
421 CCCCACCCCC AATTTTGTAT TTATTTATTT TTTAATTATT TTGTGCAGCG ATGGGGGCGG
481 GGGGGGGGGG GGGGCGCGCG CCAGGCGGGG CGGGGCGGGG CGAGGGGCGG GGCGGGGCGA
541 GGCGGAGAGG TGCGGCGGCA GCCAATCAGA GCGGCGCGCT CCGAAAGTTT CCTTTTATGG
601 CGAGGCGGCG GCGGCGGCGG CCCTATAAAA AGCGAAGCGC GCGGCGGGCG GGAGTCGTGC
661 GCGCTGCCTT CGCCCCGTGC CCCGCTCCGC CGCCGCCTCG CGCCGCCCGC CCCGGCTCTG
721 ACTGACCGCG TTACTCCCAC AGGTGAGCGG GCGGGACGGC CCTTCTCCTT CGGGCTGTAA
781 TTAGCGCTTG GTTTAATGAC GGCTTGTTTC TTTTCTGTGG CTGCGTGAAA GCCTTGAGGG
841 GCTCCGGGAG GGCCCTTTGT GCGGGGGGAG CGGCTCGGGG CTGTCCGCGG GGGGACGGCT
901 GCCTTCGGGG GGGACGGGGC AGGGCGGGGT TCGGCTTCTG GCGTGTGACC GGCGGCTCTA
961 GAGCCTCTGC TAACCATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC
1021 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGGA TCGGACCGGA AATCATAAAA
1081 AATTATTTGC TTTGTGAGCG GATAACAATT ATAATAGATT CAATTGAGGC CTCGACCACC
1141 GGGACCTTTA ATTCAACCCA ACACAATATA TTATAGTTAA ATAAGAATTA TTATCAAATC
1201 ATTTGTATAT TAATTAAAAT ACTATACTGT AAATTACATT TTATTTACAA TCAAAGGAGA
1261 TATACCATGG CGAGCGCGTG GAGCCACCCG CAGTTCGAAA AAATATCCCG GGAGCTCGTG
1321 GATCCGAATT CTCAGATCTC GGCGCGCCTG CAGGTCGACG GTACCGGTTC GAAGCTTGCG
1381 GCCGCACAGC TGTATACACG TGCAAGCCAG CCAGAACTCG CCCCGGAAGA CCCCGAGGAT
1441 CTCGAGCACC ACCATCACCA TCACCATCAC TAAGTGATTA ACCTCAGGTG CAGGCTGCCT
1501 ATCAGAAGGT GGTGGCTGGT GTGGCCAATG CCCTGGCTCA CAAATACCAC TGAGATCGAT
1561 CTTTTTCCCT CTGCCAAAAA TTATGGGGAC ATCATGAAGC CCCTTGAGCA TCTGACTTCT
1621 GGCTAATAAA GGAAATTTAT TTTCATTGCA ATAGTGTGTT GGAATTTTTT GTGTCTCTCA
1681 CTCGGAAGGA CATATGGGAG GGCAAATCAT TTAAAACATC AGAATGAGTT TTTGGTTTAG
1741 AGTTTGGCAA CATATGCCCA TATGTAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
1801 TTGAGGGGTT TTTTGCTGAA AGCATGCGGA GGAAATTCTC CTTGAAGTTT CCCTGGTGTT
1861 CAAAGTAAAG GAGTTTGCAC CAGACGCACC TCTGTTCACT GGTCCGGCGT ATTAAAACAC
1921 GATACATTGT TATTAGTACA TTTATTAAGC GCTAGATTCT GTGCGTTGTT GATTTACAGA
1981 CAATTGTTGT ACGTATTTTA ATAATTCATT AAATTTATAA TCTTTAGGGT GGTATGTTAG
2041 AGCGAAAATC AAATGATTTT CAGCGTCTTT ATATCTGAAT TTAAATATTA AATCCTCAAT
2101 AGATTTGTAA AATAGGTTTC GATTAGTTTC AAACAAGGGT TGTTTTTCCG AACCGATGGC
2161 TGGACTATCT AATGGATTTT CGCTCAACGC CACAAAACTT GCCAAATCTT GTAGCAGCAA
2221 TCTAGCTTTG TCGATATTCG TTTGTGTTTT GTTTTGTAAT AAAGGTTCGA CGTCGTTCAA
2281 AATATTATGC GCTTTTGTAT TTCTTTCATC ACTGTCGTTA GTGTACAATT GACTCGACGT
2341 AAACACGTTA AATAGAGCTT GGACATATTT AACATCGGGC GTGTTAGCTT TATTAGGCCG
2401 ATTATCGTCG TCGTCCCAAC CCTCGTCGTT AGAAGTTGCT TCCGAAGACG ATTTTGCCAT
2461 AGCCACACGA CGCCTATTAA TTGTGTCGGC TAACACGTCC GCGATCAAAT TTGTAGTTGA
2521 GCTTTTTGGA ATTATTTCTG ATTGCGGGCG TTTTTGGGCG GGTTTCAATC TAACTGTGCC
2581 CGATTTTAAT TCAGACAACA CGTTAGAAAG CGATGGTGCA GGCGGTGGTA ACATTTCAGA
2641 CGGCAAATCT ACTAATGGCG GCGGTGGTGG AGCTGATGAT AAATCTACCA TCGGTGGAGG
2701 CGCAGGCGGG GCTGGCGGCG GAGGCGGAGG CGGAGGTGGT GGCGGTGATG CAGACGGCGG
2761 TTTAGGCTCA AATGTCTCTT TAGGCAACAC AGTCGGCACC TCAACTATTG TACTGGTTTC
2821 GGGCGCCGTT TTTGGTTTGA CCGGTCTGAG ACGAGTGCGA TTTTTTTCGT TTCTAATAGC
2881 TTCCAACAAT TGTTGTCTGT CGTCTAAAGG TGCAGCGGGT TGAGGTTCCG TCGGCATTGG
2941 TGGAGCGGGC GGCAATTCAG ACATCGATGG TGGTGGTGGT GGTGGAGGCG CTGGAATGTT
3001 AGGCACGGGA GAAGGTGGTG GCGGCGGTGC CGCCGGTATA ATTTGTTCTG GTTTAGTTTG
3061 TTCGCGCACG ATTGTGGGCA CCGGCGCAGG CGCCGCTGGC TGCACAACGG AAGGTCGTCT
3121 GCTTCGAGGC AGCGCTTGGG GTGGTGGCAA TTCAATATTA TAATTGGAAT ACAAATCGTA
3181 AAAATCTGCT ATAAGCATTG TAATTTCGCT ATCGTTTACC GTGCCGATAT TTAACAACCG
3241 CTCAATGTAA GCAATTGTAT TGTAAAGAGA TTGTCTCAAG CTCGGAACGC TGCGCTCGGT
3301 CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA
3361 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG
3421 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCTGACGA GCATCACAAA
3481 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT
3541 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG
3601 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC
3661 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC
3721 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA
3781 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
3841 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC
3901 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA
3961 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA
4021 AAAGGATCTC AAGAAGATCC TTTGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
4081 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT
4141 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC
4201 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC
4261 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG
4321 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG
4381 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG
4441 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG
4501 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT
4561 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA
4621 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC
4681 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC
4741 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC
4801 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC
4861 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA
4921 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGTCC GCGCGTTTCC TGCATCTTTT
4981 AATCAAATCC CAAGATGTGT ATAAACCACC AAACTGCCAA AAAATGAAAA CTGTCGACAA
5041 GCTCTGTCCG TTTGCTGGCA ACTGCAAGGG TCTCAATCCT ATTTGTAATT ATTGAATAAT
5101 AAAACAATTA TAAATGTCAA ATTTGTTTTT TATTAACGAT ACAAACCAAA CGCAACAAGA
5161 ACATTTGTAG TATTATCTAT AATTGAAAAC GCGTAGTTAT AATCGCTGAG GTAATATTTA
5221 AAATCATTTT CAAATGATTC ACAGTTAATT TGCGACAATA TAATTTTATT TTCACATAAA
5281 CTAGACGCCT TGTCGTCTTC TTCTTCGTAT TCCTTCTCTT TTTCATTTTT CTCTTCATAA
5341 AAATTAACAT AGTTATTATC GTATCCATAT ATGTATCTAT CGTATAGAGT AAATTTTTTG
5401 TTGTCATAAA TATATATGTC TTTTTTAATG GGGTGTATAG TACCGCTGCG CATAGTTTTT
5461 CTGTAATTTA CAACAGTGCT ATTTTCTGGT AGTTCTTCGG AGTGTGTTGC TTTAATTATT
5521 AAATTTATAT AATCAATGAA TTTGGGATCG TCGGTTTTGT ACAATATGTT GCCGGCATAG
5581 TACGCAGCTT CTTCTAGTTC AATTACACCA TTTTTTAGCA GCACCGGATT AACATAACTT
5641 TCCAAAATGT TGTACGAACC GTTAAACAAA AACAGTTCAC CTCCCTTTTC TATACTATTG
5701 TCTGCGAGCA GTTGTTTGTT GTTAAAAATA ACAGCCATTG TAATGAGACG CACAAACTAA
5761 TATCACAAAC TGGAAATGTC TATCAATATA TAGTTG
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