pETBlue-2
pETBlue-2
编号 | 载体名称 |
北京华越洋VECT90063 | pETBlue-2 |
pETBlue-2 pET系列表达载体基本信息：
启动子: | T7/lac |
复制子: | ColE1 ori，F1 ori |
终止子: | T7 terminator |
质粒分类: | 大肠杆菌载体；PET系列表达质粒 |
质粒大小: | 3653bp |
质粒标签: | C-HSV，C-6×His |
原核抗性: | 氨苄青霉素Amp |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧，LB |
表达宿主: | BL21(DE3) |
诱导方式: | IPTG或乳糖及其类似物 |
5'测序引物: | T7:TAATACGACTCACTATAGGG |
3'测序引物: | T7-ter:TGCTAGTTATTGCTCAGCGG |
pETBlue-2载体质粒图谱和多克隆位点信息：
pETBlue-2载体简介：
The pETBlue™-2 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes. Screening is independent of expression because the T7lac expression promoter is in an opposed orientation relative to the E. coli promoter that mediates blue/white screening. pETBlue-2 defines the open reading frame and inserts must be cloned in-frame if expression is desired. The vector features an expanded multiple cloning site (MCS) and optional C-terminal HSV•Tag® and His•Tag® sequences. The sequence is numbered from the first base of the T7 promoter sequence.
pETBlue-2载体序列：
ORIGIN
1 TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GACTTACAAT
61 TTCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTACG GGCCTCTTCG
121 CTATTACGCC AGCTTGCGAA CGGTGGGTGC GCTGCAAGGC GATTAAGTTG GGTAACGCCA
181 GGATTCTCCC AGTCACGACG TTGTAAAACG ACGGCCAGCG AGAGATCTTG ATTGGCTAGC
241 AGAATAATTT TGTTTAACTT TAAGAAGGAG ATATACCATG GCGATATCCC GGGAGCTCGT
301 GGATCCGAAT TCTGTACAGG CGCGCCTGCA GGACGTCGAC GGTACCATCG ATACGCGTTC
361 GAAGCTTGCG GCCGCACAGC TGTATACACG TGCAAGCCAG CCAGAACTCG CTCCTGAAGA
421 CCCAGAGGAT CTCGAGCACC ACCACCACCA CCACTAATGT TAATTAAGTT GGGCGTTGTA
481 ATCATAGTCA TAATCAATAC TCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC
541 ATTAAAGCTA TTCGATGATA AGCTGTCAAA CATGATAATT CTTGAAGACG AAAGGGCCTA
601 GGCTGATAAA ACAGAATTTG CCTGGCGGCA GTAGCGCGGT GGTCCCACCT GACCCCATGC
661 CGAACTCAGA AGTGAAACGC CGTAGCGCCG ATGGTAGTGT GGGGTCTCCC CATGCGAGAG
721 TAGGGAACTG CCAGGCATCA AATAAAACGA AAGGCTCAGT CGAAAGACTG GGCCTTTCGT
781 TTTATCTGTT GTTTGTCGGT GAACGCTCTC CTGAGTAGGA CAAATCCGCC GGGAGCGGAT
841 TTGAACGTTG CGAAGCAACG GCCCGGAGGG TGGCGGGCAG GACGCCCGCC ATAAACTGCC
901 AGGCATCAAA TTAAGCAGAA GGCCATCCTG ACGGATGGCC TTTTTGCGTT TCTACAAACT
961 CTTTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTGAGCAATA ACTAGCATAA
1021 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC
1081 GGATTGGCGA ATGGGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA
1141 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC
1201 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT
1261 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG
1321 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA
1381 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT
1441 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA
1501 TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC GTTTACAATT TCTGGCGGCA
1561 CGATGGCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG
1621 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT
1681 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC
1741 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT
1801 ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG
1861 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG
1921 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC
1981 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA
2041 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG
2101 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC
2161 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA
2221 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC
2281 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG
2341 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC
2401 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC
2461 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT
2521 ACTCATACTC TTCCTTTTTC AATCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC
2581 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC
2641 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT
2701 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT
2761 ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT
2821 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT
2881 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG
2941 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA
3001 CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG
3061 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG
3121 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC
3181 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG
3241 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT
3301 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC
3361 AGCGAGTCAG TGAGCGAGGA AGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT
3421 GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA
3481 CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC
3541 TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAC
3601 CGGTGAATTG TGAGCGCTCA CAATTCTCGT GACATCATAA CGTCCCGCGA AAT
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