pGEX-1λT EcoRI/BAP
pGEX-1λT EcoRI/BAP
编号 | 载体名称 |
北京华越洋VECT90028 | pGEX-1λT EcoRI/BAP |
pGEX-1λT EcoRI/BAP pGEX系列表达载体基本信息：
启动子: | Tac/lac |
复制子: | ColE1 ori |
质粒分类: | 大肠杆菌载体；pGEX系列表达质粒 |
质粒大小: | 4947bp |
质粒标签: | N-GST, N-thrombin |
原核抗性: | 氨苄青霉素Amp |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧，LB |
表达宿主: | 大肠杆菌 |
诱导方式: | IPTG或乳糖及其类似物 |
5'测序引物: | pGEX5: GGGCTGGCAAGCCACGTTTGGTG |
3'测序引物: | pGEX3: CCGGGAGCTGCATGTGTCAGAGG |
备注: | 可用GST亲和柱纯化重组蛋白 |
pGEX-1λT EcoRI/BAP载体质粒图谱和多克隆位点信息：
pGEX-1λT EcoRI/BAP载体简介
pGEX-1λT EcoRI/BAP载体序列：
ORIGIN
1 ACGTTATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC GGAAGCTGTG
61 GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC GCACTCCCGT
121 TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC TGAAATGAGC
181 TGTTGACAAT TAATCATCGG CTCGTATAAT GTGTGGAATT GTGAGCGGAT AACAATTTCA
241 CACAGGAAAC AGTATTCATG TCCCCTATAC TAGGTTATTG GAAAATTAAG GGCCTTGTGC
301 AACCCACTCG ACTTCTTTTG GAATATCTTG AAGAAAAATA TGAAGAGCAT TTGTATGAGC
361 GCGATGAAGG TGATAAATGG CGAAACAAAA AGTTTGAATT GGGTTTGGAG TTTCCCAATC
421 TTCCTTATTA TATTGATGGT GATGTTAAAT TAACACAGTC TATGGCCATC ATACGTTATA
481 TAGCTGACAA GCACAACATG TTGGGTGGTT GTCCAAAAGA GCGTGCAGAG ATTTCAATGC
541 TTGAAGGAGC GGTTTTGGAT ATTAGATACG GTGTTTCGAG AATTGCATAT AGTAAAGACT
601 TTGAAACTCT CAAAGTTGAT TTTCTTAGCA AGCTACCTGA AATGCTGAAA ATGTTCGAAG
661 ATCGTTTATG TCATAAAACA TATTTAAATG GTGATCATGT AACCCATCCT GACTTCATGT
721 TGTATGACGC TCTTGATGTT GTTTTATACA TGGACCCAAT GTGCCTGGAT GCGTTCCCAA
781 AATTAGTTTG TTTTAAAAAA CGTATTGAAG CTATCCCACA AATTGATAAG TACTTGAAAT
841 CCAGCAAGTA TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT GGTGGCGACC
901 ATCCTCCAAA ATCGGATCTG GTTCCGCGTG GATCCCCGGA ATTCATCGTG ACTGACTGAC
961 GATCTGCCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA
1021 GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC
1081 AGCGGGTGTT GGCGGGTGTC GGGGCGCAGC CATGACCCAG TCACGTAGCG ATAGCGGAGT
1141 GTATAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT
1201 GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC
1261 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG
1321 ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC
1381 CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT
1441 GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT
1501 CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC
1561 TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT
1621 CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA
1681 GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA
1741 TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT
1801 TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA
1861 AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG
1921 CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT
1981 GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT
2041 TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC
2101 AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA
2161 TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC
2221 AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG
2281 GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC
2341 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT
2401 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT
2461 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT
2521 ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC
2581 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA
2641 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG
2701 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG
2761 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG
2821 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA
2881 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT
2941 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG
3001 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC
3061 TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC
3121 CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT
3181 TACGCATCTG TGCGGTATTT CACACCGCAT AAATTCCGAC ACCATCGAAT GGTGCAAAAC
3241 CTTTCGCGGT ATGGCATGAT AGCGCCCGGA AGAGAGTCAA TTCAGGGTGG TGAATGTGAA
3301 ACCAGTAACG TTATACGATG TCGCAGAGTA TGCCGGTGTC TCTTATCAGA CCGTTTCCCG
3361 CGTGGTGAAC CAGGCCAGCC ACGTTTCTGC GAAAACGCGG GAAAAAGTGG AAGCGGCGAT
3421 GGCGGAGCTG AATTACATTC CCAACCGCGT GGCACAACAA CTGGCGGGCA AACAGTCGTT
3481 GCTGATTGGC GTTGCCACCT CCAGTCTGGC CCTGCACGCG CCGTCGCAAA TTGTCGCGGC
3541 GATTAAATCT CGCGCCGATC AACTGGGTGC CAGCGTGGTG GTGTCGATGG TAGAACGAAG
3601 CGGCGTCGAA GCCTGTAAAG CGGCGGTGCA CAATCTTCTC GCGCAACGCG TCAGTGGGCT
3661 GATCATTAAC TATCCGCTGG ATGACCAGGA TGCCATTGCT GTGGAAGCTG CCTGCACTAA
3721 TGTTCCGGCG TTATTTCTTG ATGTCTCTGA CCAGACACCC ATCAACAGTA TTATTTTCTC
3781 CCATGAAGAC GGTACGCGAC TGGGCGTGGA GCATCTGGTC GCATTGGGTC ACCAGCAAAT
3841 CGCGCTGTTA GCGGGCCCAT TAAGTTCTGT CTCGGCGCGT CTGCGTCTGG CTGGCTGGCA
3901 TAAATATCTC ACTCGCAATC AAATTCAGCC GATAGCGGAA CGGGAAGGCG ACTGGAGTGC
3961 CATGTCCGGT TTTCAACAAA CCATGCAAAT GCTGAATGAG GGCATCGTTC CCACTGCGAT
4021 GCTGGTTGCC AACGATCAGA TGGCGCTGGG CGCAATGCGC GCCATTACCG AGTCCGGGCT
4081 GCGCGTTGGT GCGGATATCT CGGTAGTGGG ATACGACGAT ACCGAAGACA GCTCATGTTA
4141 TATCCCGCCG TTAACCACCA TCAAACAGGA TTTTCGCCTG CTGGGGCAAA CCAGCGTGGA
4201 CCGCTTGCTG CAACTCTCTC AGGGCCAGGC GGTGAAGGGC AATCAGCTGT TGCCCGTCTC
4261 ACTGGTGAAA AGAAAAACCA CCCTGGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT
4321 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC
4381 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC
4441 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT
4501 ATGACCATGA TTACGGATTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT
4561 GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC
4621 GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGCGC
4681 TTTGCCTGGT TTCCGGCACC AGAAGCGGTG CCGGAAAGCT GGCTGGAGTG CGATCTTCCT
4741 GAGGCCGATA CTGTCGTCGT CCCCTCAAAC TGGCAGATGC ACGGTTACGA TGCGCCCATC
4801 TACACCAACG TAACCTATCC CATTACGGTC AATCCGCCGT TTGTTCCCAC GGAGAATCCG
4861 ACGGGTTGTT ACTCGCTCAC ATTTAATGTT GATGAAAGCT GGCTACAGGA AGGCCAGACG
4921 CGAATTATTT TTGATGGCGT TGGAATT
//
pGEX-1λT EcoRI/BAP其他相关pGEX系列表达载体：
