pQE-TriSystem-7
pQE-TriSystem-7
编号 | 载体名称 |
北京华越洋VECT90068 | pQE-TriSystem-7 |
pQE-TriSystem-7 pQE系列表达载体基本信息：
启动子: | p10 |
复制子: | ColE1 ori |
终止子: | T7 terminator |
质粒分类: | 大肠杆菌载体；pQE系列表达质粒 |
质粒大小: | 5250bp |
原核抗性: | 氨苄青霉素Amp |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧，LB |
表达宿主: | M15 |
诱导方式: | IPTG或乳糖及其类似物 |
pQE-TriSystem-7载体质粒图谱和多克隆位点信息：
pQE-TriSystem-7载体简介
pQE-TriSystem-7载体序列：
ORIGIN
1 TCCTGCATCT TTTAATCAAA TCCCAAGATG TGTATAAACG CGCCGGTATG TACAGGAAGA
61 GGTTTATACT AAACTGTTAC ATTGCAAACG TGGTTTCGTG TGCCAAGTGT GAAAACCGAT
121 GTTTAATCAA GGCTCTGACG CATTTCTACA ACCACGACTC CAAGTGTGTG GGTGAAGTCA
181 TGCATCTTTT AATCAAATCC CAAGATGTGT ATAAACCACC AAACTGCCAA AAAATGAAAA
241 CTGTCGACAA GCTCTGTCCG TTTGCTGGCA ACTGCAAGGG TCTCAATCCT ATTTGTAATT
301 ATTGAATAAT AAAACAATTA TAAATGTCAA ATTTGTTTTT TATTAACGAT ACAAACCAAA
361 CGCAACAAGA ACATTTGTAG TATTATCTAT AATTGAAAAC GCGTAGTTAT AATCGCTGAG
421 GTAATATTTA AAATCATTTT CAAATGATTC ACAGTTAATT TGCGACAATA TAATTTTATT
481 TTCACATAAA CTAGACGCCT TGTCGTCTTC TTCTTCGTAT TCCTTCTCTT TTTCATTTTT
541 CTCTTCATAA AAATTAACAT AGTTATTATC GTATCCATAT ATGTATCTAT CGTATAGAGT
601 AAATTTTTTG TTGTCATAAA TATATATGTC TTTTTTAATG GGGTGTATAG TACCGCTGCG
661 CATAGTTTTT CTGTAATTTA CAACAGTGCT ATTTTCTGGT AGTTCTTCGG AGTGTGTTGC
721 TTTAATTATT AAATTTATAT AATCAATGAA TTTGGGATCG TCGGTTTTGT ACAATATGTT
781 GCCGGCATAG TACGCAGCTT CTTCTAGTTC AATTACACCA TTTTTTAGCA GCACCGGATT
841 AACATAACTT TCCAAAATGT TGTACGAACC GTTAAACAAA AACAGTTCAC CTCCCTTTTC
901 TATACTATTG TCTGCGAGCA GTTGTTTGTT GTTAAAAATA ACAGCCATTG TAATGAGACG
961 CACAAACTAA TATCACAAAC TGGAAATGTC TATCAATATA TAGTTGCTGA TGGCCGGCCG
1021 TAATGAGACG CACAAACTAA TATCACAAAC TGGAAATGTC TATCAATATA TAGTTGCTCT
1081 AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC
1141 GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG
1201 ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA
1261 TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA
1321 AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC
1381 ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC
1441 ATGCATGGTC GAGGTGAGCC CCACGTTCTG CTTCACTCTC CCCATCTCCC CCCCCTCCCC
1501 ACCCCCAATT TTGTATTTAT TTATTTTTTA ATTATTTTGT GCAGCGATGG GGGCGGGGGG
1561 GGGGGGGGGG CGCGCGCCAG GCGGGGCGGG GCGGGGCGAG GGGCGGGGCG GGGCGAGGCG
1621 GAGAGGTGCG GCGGCAGCCA ATCAGAGCGG CGCGCTCCGA AAGTTTCCTT TTATGGCGAG
1681 GCGGCGGCGG CGGCGGCCCT ATAAAAAGCG AAGCGCGCGG CGGGCGGGAG TCGCTGCGCG
1741 CTGCCTTCGC CCCGTGCCCC GCTCCGCCGC CGCCTCGCGC CGCCCGCCCC GGCTCTGACT
1801 GACCGCGTTA CTCCCACAGG TGAGCGGGCG GGACGGCCCT TCTCCTTCGG GCTGTAATTA
1861 GCGCTTGGTT TAATGACGGC TTGTTTCTTT TCTGTGGCTG CGTGAAAGCC TTGAGGGGCT
1921 CCGGGAGGGC CCTTTGTGCG GGGGGAGCGG CTCGGGGCTG TCCGCGGGGG GACGGCTGCC
1981 TTCGGGGGGG ACGGGGCAGG GCGGGGTTCG GCTTCTGGCG TGTGACCGGC GGCTCTAGAG
2041 CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC AACGTGCTGG
2101 TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTGGATCG GACCGAAATT AATACGACTC
2161 ACTATAGGGG AATTGTGAGC GGATAACAAT TCCCCGGAGT TAATCCGGGA CCTTTAATTC
2221 AACCCAACAC AATATATTAT AGTTAAATAA GAATTATTAT CAAATCATTT GTATATTAAT
2281 TAAAATACTA TACTGTAAAT TACATTTTAT TTACAATCAA AGGAGATATA CCATGGCGAT
2341 ATCCCGGGTG GATCCGAATT CTCAGATCTC GGCGCGCCTG CAGGGTTCGA AGCTTATACT
2401 CGAGAAGCGT TGAAATAGCG TACAAGTCGA GCACCACCAT CACCATCACC ATCACTAAGT
2461 GATTAACCTC AGGTGCAGGC TGCCTATCAG AAGGTGGTGG CTGGTGTGGC CAATGCCCTG
2521 GCTCACAAAT ACCACTGAGA TCGATCTTTT TCCCTCTGCC AAAAATTATG GGGACATCAT
2581 GAAGCCCCTT GAGCATCTGA CTTCTGGCTA ATAAAGGAAA TTTATTTTCA TTGCAATAGT
2641 GTGTTGGAAT TTTTTGTGTC TCTCACTCGG AAGGACATAT GGGAGGGCAA ATCATTTAAA
2701 ACATCAGAAT GAGTATTTGG TTTAGAGTTT GGCAACATAT GCCCATATGT AACTAGCATA
2761 ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGCAT GCGGAGGAAA
2821 TTCTCCTTGA AGTTTCCCTG GTGTTCAAAG TAAAGGAGTT TGCACCAGAC GCACCTCTGT
2881 TCACTGGTCC GGCGTATTAA AACACGATAC ATTGTTATTA GTACATTTAT TAAGCGCTAG
2941 ATTCTGTGCG TTGTTGATTT ACAGACAATT GTTGTACGTA TTTTAATAAT TCATTAAATT
3001 TATAATCTTT AGGGTGGTAT GTTAGAGCGA AAATCAAATG ATTTTCAGCG TCTTTATATC
3061 TGAATTTAAA TATTAAATCC TCAATAGATT TGTAAAATAG GTTTCGATTA GTTTCAAACA
3121 AGGGTTGTTT TTCCGAACCG ATGGCTGGAC TATCTAATGG ATTTTCGCTC AACGCCACAA
3181 AACTTGCCAA ATCTTGTAGC AGCAATCTAG CTTTGTCGAT ATTCGTTTGT GTTTTGTTTT
3241 GTAATAAAGG TTCGACGTCG TTCAAAATAT TATGCGCTTT TGTATTTCTT TCATCACTGT
3301 CGTTAGTGTA CAATTGACTC GACGTAAACA CGTTAAATAG AGCTTGGACA TATTTAACAT
3361 CGGGCGTGTT AGCTTTATTA GGCCGATTAT CGTCGTCGTC CCAACCCTCG TCGTTAGAAG
3421 TTGCTTCCGA AGACGATTTT GCCATAGCCA CACGACGCCT ATTAATTGTG TCGGCTAACA
3481 CGTCCGCGAT CAAATTTGTA GTTGAGCTTT TTGGAATTGC GATCGCATAA CTTCGTATAG
3541 CATACATTAT ACGAAGTTAT AAGCTCGGAA CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG
3601 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG
3661 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC
3721 TGGCGTTTTT CCATAGGCTC CGCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
3781 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
3841 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
3901 GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
3961 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
4021 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
4081 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
4141 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
4201 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
4261 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
4321 TCCTTTGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT
4381 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT
4441 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG
4501 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT
4561 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT
4621 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG
4681 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA
4741 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT
4801 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT
4861 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC
4921 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA
4981 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT
5041 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT
5101 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA
5161 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT
5221 ATCAGGGTTA TTGTCTCATG TCCGCGCGTT
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