北京华越洋生物
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首页    载体及质粒    pDrive
pDrive

pDrive

pDrive

pDrive

 



编号

载体名称

北京华越洋VECT90156

pDrive

 

pDrive载体基本信息:

启动子:

T7

复制子:

ColE1 oriF1 ori

质粒分类:

大肠杆菌载体;蛋白表达质粒

质粒大小:

3851bp

原核抗性:

氨苄青霉素Amp

克隆菌株:

DH5α

培养条件:

37,有氧,LB

表达宿主:

大肠杆菌

备注:

表达质粒

 

pDrive载体质粒图谱和多克隆位点信息:

pDrive载体简介

pDrive载体序列:

ORIGIN
        1 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
       61 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT
      121 CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT
      181 TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCTCTA
      241 ATACGACTCA CTATAGGGAA AGCTCGGTAC CACGCATGCT GCAGACGCGT TACGTATCGG
      301 ATCCAGAATT CGTGATATCT GAATTCGTCG ACAAGCTTCT CGAGCCTAGG CTAGCTCTAG
      361 ACCACACGTG TGGGGGCCCG AGCTCGCGGC CGCTGTATTC TATAGTGTCA CCTAAATGGC
      421 CGCACAATTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC
      481 AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC
      541 GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGAAA TTGTAAGCGT
      601 TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA
      661 GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT
      721 TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG
      781 AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT
      841 GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC
      901 TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG
      961 CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT
     1021 TAATGCGCCG CTACAGGGCG CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC
     1081 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG
     1141 ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC
     1201 CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT
     1261 GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT
     1321 CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC
     1381 TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT
     1441 CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA
     1501 GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA
     1561 TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT
     1621 TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA
     1681 AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG
     1741 CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT
     1801 GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT
     1861 TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC
     1921 AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA
     1981 TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC
     2041 AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG
     2101 GATCTAGGTG AAGATCCTTT TTGATAATCT CATGAACAAT AAAACTGTCT GCTTACATAA
     2161 ACAGTAATAC AAGGGGTGTT ATGAGCCATA TTCAACGGGA AACGTCTTGC TCTAGGCCGC
     2221 GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA ATGGGCTCGC GATAATGTCG
     2281 GGCAATCAGG TGCGACAATC TATCGATTGT ATGGGAAGCC CGATGCGCCA GAGTTGTTTC
     2341 TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA TGAGATGGTC AGACTAAACT
     2401 GGCTGACGGA ATTTATGCCT CTTCCGACCA TCAAGCATTT TATCCGTACT CCTGATGATG
     2461 CATGGTTACT CACCACTGCG ATCCCCGGGA AAACAGCATT CCAGGTATTA GAAGAATATC
     2521 CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT CCTGCGCCGG TTGCATTCGA
     2581 TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT TCGTCTCGCT CAGGCGCAAT
     2641 CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA TGACGAGCGT AATGGCTGGC
     2701 CTGTTGAACA AGTCTGGAAA GAAATGCATA AACTTTTGCC ATTCTCACCG GATTCAGTCG
     2761 TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA CGAGGGGAAA TTAATAGGTT
     2821 GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA
     2881 ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG
     2941 ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT CGATGAGTTT TTCTAAGAAT
     3001 TAATTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA
     3061 GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA
     3121 ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT
     3181 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG
     3241 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA
     3301 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA
     3361 AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG
     3421 CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA
     3481 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA
     3541 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC
     3601 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC
     3661 CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT
     3721 GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT
     3781 GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG
     3841 GAAGCGGAAG A//

pDrive其他相关其他大肠杆菌载体:

pETBlue-2

pQE-30   UA   (linearized)

pET30a-EcoRV

pQE-22

pET28a-SUMO

pQE-19

pET28a-OFP

pQE-18

pET28a-mCherry

pQE-17

pET28a-EYFP

pQE-15

pET28a-EGFP

pQE-14

pET28a-ECFP

pQE-13

pET28a-EBFP

pQE-12

pET28a-DsRed2

pQE-11

pTrc-CKS

pQE-10

pMBP-P

pQE-8

pMBP-C

pQE-7

pLpp-OmpA

pQE-6

pLLP-STII/Plpp-STII

pQE-5

pET-Trx

pQE-4

pETM-30

pQE-3

pETM-11

pCold-SUMO-His-FLAG

pET-GST

pCold-SUMO-His-FLAG

pET-DsbA

pCold-SUMO

pET22b-EGFP

pCold-NusA

TAGZyme     pQE-2

pCold-MBP

TAGZyme     pQE-1

pCold-DsbA

pQE-TriSystem-7

pUC57-Kan

pQE-TriSystem-6

pUC57-Simple

pQE-TriSystem-5

pUC57

pQE-TriSystem     Strep

pUC119

pQE-TriSystem     His-Strep2

pUC118

pQE-TriSystem     His-Strep1

pJET1.2   T   Recombined

pQE-T7-2

pMD-19TSimple

pQE-T7-1

pGEM-3Zf(+)

pQE-T7(cloning     region)

pBluescript     II SK(+)

pQE-100     DoubleTaq

pBluescript     II KS(+)

pQE-82L-Kan

pUC19

pQE-81L-Kan

pUC18

pQE-80L-Kan

pMD-18T

pQE-80

pUC57-Tac-mCherry

pQE-52

pTYB21

pQE-51

pTYB4

pQE-50

pTrcHisC

pQE-42

pTrcHisB

pQE-40

pTrcHisA

pQE-30   Xa

pTrcHis2C

pQE-30   UA

pTrcHis2B

pTrcHis2A

pBAD/Myc-HisA

pRSETC

pBAD/His     /LacZ

pRSETB

pBAD/His-C

pMCSG19

pBAD/His-B

pMCSG9

pBAD/His-A

pLAFR-3

pBAD30

pEGFP-Lac

pGEX-Zsgreen1

pDrive

pGEX-TagRFP

pBV222

pGEX-His

pBAD/Myc-HisC

pGEX-20T

pBAD/Myc-HisB

pGEX-1λT     EcoRI/BAP