pQE-TriSystem-5
pQE-TriSystem-5
编号 | 载体名称 |
北京华越洋VECT90070 | pQE-TriSystem-5 |
pQE-TriSystem-5 pQE系列表达载体基本信息：
启动子: | p10 |
复制子: | ColE1 ori |
终止子: | T7 terminator |
质粒分类: | 大肠杆菌载体；pQE系列表达质粒 |
质粒大小: | 5292bp |
原核抗性: | 氨苄青霉素Amp |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧，LB |
表达宿主: | M15 |
诱导方式: | IPTG或乳糖及其类似物 |
pQE-TriSystem-5载体质粒图谱和多克隆位点信息：
pQE-TriSystem-5载体简介
pQE-TriSystem-5载体序列：
ORIGIN
1 TCCTGCATCT TTTAATCAAA TCCCAAGATG TGTATAAACG CGCCGGTATG TACAGGAAGA
61 GGTTTATACT AAACTGTTAC ATTGCAAACG TGGTTTCGTG TGCCAAGTGT GAAAACCGAT
121 GTTTAATCAA GGCTCTGACG CATTTCTACA ACCACGACTC CAAGTGTGTG GGTGAAGTCA
181 TGCATCTTTT AATCAAATCC CAAGATGTGT ATAAACCACC AAACTGCCAA AAAATGAAAA
241 CTGTCGACAA GCTCTGTCCG TTTGCTGGCA ACTGCAAGGG TCTCAATCCT ATTTGTAATT
301 ATTGAATAAT AAAACAATTA TAAATGTCAA ATTTGTTTTT TATTAACGAT ACAAACCAAA
361 CGCAACAAGA ACATTTGTAG TATTATCTAT AATTGAAAAC GCGTAGTTAT AATCGCTGAG
421 GTAATATTTA AAATCATTTT CAAATGATTC ACAGTTAATT TGCGACAATA TAATTTTATT
481 TTCACATAAA CTAGACGCCT TGTCGTCTTC TTCTTCGTAT TCCTTCTCTT TTTCATTTTT
541 CTCTTCATAA AAATTAACAT AGTTATTATC GTATCCATAT ATGTATCTAT CGTATAGAGT
601 AAATTTTTTG TTGTCATAAA TATATATGTC TTTTTTAATG GGGTGTATAG TACCGCTGCG
661 CATAGTTTTT CTGTAATTTA CAACAGTGCT ATTTTCTGGT AGTTCTTCGG AGTGTGTTGC
721 TTTAATTATT AAATTTATAT AATCAATGAA TTTGGGATCG TCGGTTTTGT ACAATATGTT
781 GCCGGCATAG TACGCAGCTT CTTCTAGTTC AATTACACCA TTTTTTAGCA GCACCGGATT
841 AACATAACTT TCCAAAATGT TGTACGAACC GTTAAACAAA AACAGTTCAC CTCCCTTTTC
901 TATACTATTG TCTGCGAGCA GTTGTTTGTT GTTAAAAATA ACAGCCATTG TAATGAGACG
961 CACAAACTAA TATCACAAAC TGGAAATGTC TATCAATATA TAGTTGCTGA TGGCCGGCCG
1021 TAATGAGACG CACAAACTAA TATCACAAAC TGGAAATGTC TATCAATATA TAGTTGCTCT
1081 AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC
1141 GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG
1201 ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA
1261 TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA
1321 AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC
1381 ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC
1441 ATGCATGGTC GAGGTGAGCC CCACGTTCTG CTTCACTCTC CCCATCTCCC CCCCCTCCCC
1501 ACCCCCAATT TTGTATTTAT TTATTTTTTA ATTATTTTGT GCAGCGATGG GGGCGGGGGG
1561 GGGGGGGGGG CGCGCGCCAG GCGGGGCGGG GCGGGGCGAG GGGCGGGGCG GGGCGAGGCG
1621 GAGAGGTGCG GCGGCAGCCA ATCAGAGCGG CGCGCTCCGA AAGTTTCCTT TTATGGCGAG
1681 GCGGCGGCGG CGGCGGCCCT ATAAAAAGCG AAGCGCGCGG CGGGCGGGAG TCGCTGCGCG
1741 CTGCCTTCGC CCCGTGCCCC GCTCCGCCGC CGCCTCGCGC CGCCCGCCCC GGCTCTGACT
1801 GACCGCGTTA CTCCCACAGG TGAGCGGGCG GGACGGCCCT TCTCCTTCGG GCTGTAATTA
1861 GCGCTTGGTT TAATGACGGC TTGTTTCTTT TCTGTGGCTG CGTGAAAGCC TTGAGGGGCT
1921 CCGGGAGGGC CCTTTGTGCG GGGGGAGCGG CTCGGGGCTG TCCGCGGGGG GACGGCTGCC
1981 TTCGGGGGGG ACGGGGCAGG GCGGGGTTCG GCTTCTGGCG TGTGACCGGC GGCTCTAGAG
2041 CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC AACGTGCTGG
2101 TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTGGATCG GACCGAAATT AATACGACTC
2161 ACTATAGGGG AATTGTGAGC GGATAACAAT TCCCCGGAGT TAATCCGGGA CCTTTAATTC
2221 AACCCAACAC AATATATTAT AGTTAAATAA GAATTATTAT CAAATCATTT GTATATTAAT
2281 TAAAATACTA TACTGTAAAT TACATTTTAT TTACAATCAA AGGAGATATA CCATGAAGCA
2341 CCACCACCAT CACCATCATC ACCACCATCA GTGAAGAGCG ATATCCCGGG TGGATCCGAA
2401 TTCTCAGATC TCGGCGCGCC TGCAGGGTTC GAAGCTTATA CTCGAGAAGC GTTGAAATAG
2461 CGTACAAGTC GAGCACCACC ATCACCATCA CCATCACTAA GTGATTAACC TCAGGTGCAG
2521 GCTGCCTATC AGAAGGTGGT GGCTGGTGTG GCCAATGCCC TGGCTCACAA ATACCACTGA
2581 GATCGATCTT TTTCCCTCTG CCAAAAATTA TGGGGACATC ATGAAGCCCC TTGAGCATCT
2641 GACTTCTGGC TAATAAAGGA AATTTATTTT CATTGCAATA GTGTGTTGGA ATTTTTTGTG
2701 TCTCTCACTC GGAAGGACAT ATGGGAGGGC AAATCATTTA AAACATCAGA ATGAGTATTT
2761 GGTTTAGAGT TTGGCAACAT ATGCCCATAT GTAACTAGCA TAACCCCTTG GGGCCTCTAA
2821 ACGGGTCTTG AGGGGTTTTT TGCTGAAAGC ATGCGGAGGA AATTCTCCTT GAAGTTTCCC
2881 TGGTGTTCAA AGTAAAGGAG TTTGCACCAG ACGCACCTCT GTTCACTGGT CCGGCGTATT
2941 AAAACACGAT ACATTGTTAT TAGTACATTT ATTAAGCGCT AGATTCTGTG CGTTGTTGAT
3001 TTACAGACAA TTGTTGTACG TATTTTAATA ATTCATTAAA TTTATAATCT TTAGGGTGGT
3061 ATGTTAGAGC GAAAATCAAA TGATTTTCAG CGTCTTTATA TCTGAATTTA AATATTAAAT
3121 CCTCAATAGA TTTGTAAAAT AGGTTTCGAT TAGTTTCAAA CAAGGGTTGT TTTTCCGAAC
3181 CGATGGCTGG ACTATCTAAT GGATTTTCGC TCAACGCCAC AAAACTTGCC AAATCTTGTA
3241 GCAGCAATCT AGCTTTGTCG ATATTCGTTT GTGTTTTGTT TTGTAATAAA GGTTCGACGT
3301 CGTTCAAAAT ATTATGCGCT TTTGTATTTC TTTCATCACT GTCGTTAGTG TACAATTGAC
3361 TCGACGTAAA CACGTTAAAT AGAGCTTGGA CATATTTAAC ATCGGGCGTG TTAGCTTTAT
3421 TAGGCCGATT ATCGTCGTCG TCCCAACCCT CGTCGTTAGA AGTTGCTTCC GAAGACGATT
3481 TTGCCATAGC CACACGACGC CTATTAATTG TGTCGGCTAA CACGTCCGCG ATCAAATTTG
3541 TAGTTGAGCT TTTTGGAATT GCGATCGCAT AACTTCGTAT AGCATACATT ATACGAAGTT
3601 ATAAGCTCGG AACGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA
3661 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA
3721 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC
3781 TCCGCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
3841 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
3901 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
3961 TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
4021 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
4081 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
4141 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
4201 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
4261 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
4321 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGT TACCAATGCT
4381 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC
4441 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA
4501 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG
4561 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT
4621 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA
4681 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT
4741 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT
4801 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG
4861 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG
4921 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG
4981 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA
5041 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT
5101 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT
5161 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT
5221 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA
5281 TGTCCGCGCG TT
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