pDONR221-P4RP3R
pDONR221-P4RP3R
编号 | 载体名称 |
北京华越洋VECT511135 | pDONR221-P4RP3R |
pDONR221-P4RP3R Gateway载体基本信息：
载体名称: | pDONR221-P4RP3R |
质粒类型: | Mltisite-Gateway载体；DONR载体 |
高拷贝/低拷贝: | 低拷贝 |
启动子: | 无 |
克隆方法: | Gateway；BP反应 |
载体大小: | 4717 bp |
5' 测序引物及序列: | M13/pUC fwd 5'CCCAGTCACGACGTTGTAAAACG 3' |
3' 测序引物及序列: | M13/pUC rev 5' AGCGGATAACAATTTCACACAGG 3' |
载体标签: | 无 |
载体抗性: | 卡那霉素，氯霉素（仅空载体） |
真核筛选标记: | 无 |
克隆菌株: | 阳性筛选用DB3.1，阴性筛选用OmniMAX2-T1 |
备注: |
pDONR221-P5P2载体是MultiSite Gateway载体， |
病毒/非病毒: | 非病毒 |
pDONR221-P4RP3R载体质粒图谱和多克隆位点信息：
pDONR221-P4RP3R载体简介：
pDONR221-P4RP3R载体序列：
ORIGIN
1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
541 AACGACGGCC AGTCTTAAGC TCGGGCCCCT ACAGGTCACT AATACCATCT AAGTAGTTGA
601 TTCATAGTGA CTGGATATGT TGTGTTTTAC AGTATTATGT AGTCTGTTTT TTATGCAAAA
661 TCTAATTTAA TATATTGATA TTTATATCAT TTTACGTTTC TCGTTCAACT TTTCTATACA
721 AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG CAACGAACAG GTCACTATCA
781 GTCAAAATAA AATCATTATT TGTCGACCGA CAGCCTTCCA AATGTTCTTC TCAAACGGAA
841 TCGTCGTATC CAGCCTACTC GCTATTGTCC TCAATGCCGT ATTAAATCAT AAAAAGAAAT
901 AAGAAAAAGA GGTGCGAGCC TCTTTTTTGT GTGACAAAAT AAAAACATCT ACCTATTCAT
961 ATACGCTAGT GTCATAGTCC TGAAAATCAT CTGCATCAAG AACAATTTCA CAACTCTTAT
1021 ACTTTTCTCT TACAAGTCGT TCGGCTTCAT CTGGATTTTC AGCCTCTATA CTTACTAAAC
1081 GTGATAAAGT TTCTGTAATT TCTACTGTAT CGACCTGCAG ACTGGCTGTG TATAAGGGAG
1141 CCTGACATTT ATATTCCCCA GAACATCAGG TTAATGGCGT TTTTGATGTC ATTTTCGCGG
1201 TGGCTGAGAT CAGCCACTTC TTCCCCGATA ACGGAGACCG GCACACTGGC CATATCGGTG
1261 GTCATCATGC GCCAGCTTTC ATCCCCGATA TGCACCACCG GGTAAAGTTC ACGGGAGACT
1321 TTATCTGACA GCAGACGTGC ACTGGCCAGG GGGATCACCA TCCGTCGCCC GGGCGTGTCA
1381 ATAATATCAC TCTGTACATC CACAAACAGA CGATAACGGC TCTCTCTTTT ATAGGTGTAA
1441 ACCTTAAACT GCATTTCACC AGCCCCTGTT CTCGTCAGCA AAAGAGCCGT TCATTTCAAT
1501 AAACCGGGCG ACCTCAGCCA TCCCTTCCTG ATTTTCCGCT TTCCAGCGTT CGGCACGCAG
1561 ACGACGGGCT TCATTCTGCA TGGTTGTGCT TACCAGACCG GAGATATTGA CATCATATAT
1621 GCCTTGAGCA ACTGATAGCT GTCGCTGTCA ACTGTCACTG TAATACGCTG CTTCATAGCA
1681 TACCTCTTTT TGACATACTT CGGGTATACA TATCAGTATA TATTCTTATA CCGCAAAAAT
1741 CAGCGCGCAA ATACGCATAC TGTTATCTGG CTTTTAGTAA GCCGGATCCA CGCGGCGTTT
1801 ACGCCCCCCC TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG
1861 GAAGCCATCA CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC
1921 TTGCGTATAA TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC
1981 GTTTAAATCA AAACTGGTGA AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC
2041 AATAAACCCT TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA
2101 TATGTGTAGA AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC
2161 AGTTTGCTCA TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC
2221 GTCTTTCATT GCCATACGGA ATTCCGGATG AGCATTCATC AGGCGGGCAA GAATGTGAAT
2281 AAAGGCCGGA TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC
2341 CAGCTGAACG GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC
2401 TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT TCTCCATTTT
2461 AGCTTCCTTA GCTCCTGAAA ATCTCGATAA CTCAAAAAAT ACGCCCGGTA GTGATCTTAT
2521 TTCATTATGG TGAAAGTTGG AACCTCTTAC GTGCCGATCA ACGTCTCATT TTCGCCAAAA
2581 GTTGGCCCAG GGCTTCCCGG TATCAACAGG GACACCAGGA TTTATTTATT CTGCGAAGTG
2641 ATCTTCCGTC ACAGGTATTT ATTCGGCGCA AAGTGCGTCG GGTGATGCTG CCAACTTAGT
2701 CGACAAATAA TGATTTTATT TTGACTGATA GTGACCTGTT CGTTGCAACA AATTGATGAG
2761 CAATGCTTTT TTATAATGCC AACTTTGTAT AATAAAGTTG AACGAGAAAC GTAAAATGAT
2821 ATAAATATCA ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA
2881 ACACAACATA TCCAGTCACT ATGAATCAAC TACTTAGATG GTATTAGTGA CCTGTACTGC
2941 AGGGCGGCCG CGATATCCCC TATAGTGAGT CGTATTACAT GGTCATAGCT GTTTCCTGGC
3001 AGCTCTGGCC CGTGTCTCAA AATCTCTGAT GTTACATTGC ACAAGATAAA AATATATCAT
3061 CATGAACAAT AAAACTGTCT GCTTACATAA ACAGTAATAC AAGGGGTGTT ATGAGCCATA
3121 TTCAACGGGA AACGTCGAGG CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT
3181 ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGC TTGTATGGGA
3241 AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA
3301 CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC
3361 ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC TGCGATCCCC GGAAAAACAG
3421 CATTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG
3481 TGTTCCTGCG CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG
3541 TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT
3601 TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAACTTT
3661 TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT
3721 TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT
3781 ACCAGGATCT TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC
3841 GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA
3901 TGCTCGATGA GTTTTTCTAA TCAGAATTGG TTAATTGGTT GTAACACTGG CAGAGCATTA
3961 CGCTGACTTG ACGGGACGGC GCAAGCTCAT GACCAAAATC CCTTAACGTG AGTTACGCGT
4021 CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT
4081 TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT
4141 TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA
4201 TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG
4261 CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA
4321 AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG
4381 GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA
4441 GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA
4501 GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA
4561 ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT
4621 TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC
4681 GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTT
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pDONR222其他相关Gateway载体：
