北京华越洋生物
13581845453(微信)
7*24h全国热线:
15011481284
首页    载体及质粒    pENTR-R4-pLac-Spec-R3
pENTR-R4-pLac-Spec-R3Map

pENTR-R4-pLac-Spec-R3

pENTR-R4-pLac-Spec-R3

pENTR-R4-pLac-Spec-R3

 



编号

载体名称

北京华越洋VECT511155

pENTR-R4-pLac-Spec-R3

 

pENTR-R4-pLac-Spec-R3  Gateway载体基本信息:

载体名称:

pENTR-R4-pLac-Spec-R3

质粒类型:

多位点Gateway载体;入门载体

高拷贝/低拷贝:

高拷贝

启动子:

克隆方法:

限制性内切酶;多克隆位点

载体大小:

3889 bp

5' 测序引物及序列:

pENTR-F:   CTACAAACTCTTCCTGTTAGTTAG

3' 测序引物及序列:

pENTR-R:   ATGGCTCATAACACCCCTTG

载体标签:

载体抗性:

卡那霉素

真核筛选标记:

克隆菌株:

DH5aTOP10感受态细胞

病毒/非病毒:

非病毒

 

pENTR-R4-pLac-Spec-R3载体质粒图谱和多克隆位点信息:

pENTR-R4-pLac-Spec-R3载体简介

pENTR-R4-pLac-Spec-R3载体序列:

ORIGIN

        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA

       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA

      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA

      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC

      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA

      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC

      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA

      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG

      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA

      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCT ACAGGTCACT AATACCATCT AAGTAGTTGA

      601 TTCATAGTGA CTGGATATGT TGTGTTTTAC AGTATTATGT AGTCTGTTTT TTATGCAAAA

      661 TCTAATTTAA TATATTGATA TTTATATCAT TTTACGTTTC TCGTTCAACT TTTCTATACA

      721 AAGTTGGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA

      781 TATTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GCATCGCTCA

      841 CGCAACTGGT CCAGAACCTT GACCGAACGC AGCGGTGGTA ACGGCGCAGT GGCGGTTTTC

      901 ATGGCTTGTT ATGACTGTTT TTTTGGGGTA CAGTCTATGC CTCGGGCATC CAAGCAGCAA

      961 GCGCGTTACG CCGTGGGTCG ATGTTTGATG TTATGGAGCA GCAACGATGT TACGCAGCAG

     1021 GGCAGTCGCC CTAAAACAAA GTTAAACATT ATGAGGGAAG CGGTGATCGC CGAAGTATCG

     1081 ACTCAACTAT CAGAGGTAGT TGGCGTCATC GAGCGCCATC TCGAACCGAC GTTGCTGGCC

     1141 GTACATTTGT ACGGCTCCGC AGTGGATGGC GGCCTGAAGC CACACAGTGA TATTGATTTG

     1201 CTGGTTACGG TGACCGTAAG GCTTGATGAA ACAACGCGGC GAGCTTTGAT CAACGACCTT

     1261 TTGGAAACTT CGGCTTCCCC TGGAGAGAGC GAGATTCTCC GCGCTGTAGA AGTCACCATT

     1321 GTTGTGCACG ACGACATCAT TCCGTGGCGT TATCCAGCTA AGCGCGAACT GCAATTTGGA

     1381 GAATGGCAGC GCAATGACAT TCTTGCAGGT ATCTTCGAGC CAGCCACGAT CGACATTGAT

     1441 CTGGCTATCT TGCTGACAAA AGCAAGGGAA CATAGCGTTG CCTTGGTAGG TCCAGCGGCG

     1501 GAGGAACTCT TTGATCCGGT TCCTGAACAG GATCTATTTG AGGCGCTAAA TGAAACCTTA

     1561 ACGCTATGGA ACTCGCCGCC CGACTGGGCT GGCGATGAGC GAAATGTAGT GCTTACGTTG

     1621 TCCCGCATTT GGTACAGCGC AGTAACCGGC AAAATCGCGC CGAAGGATGT CGCTGCCGAC

     1681 TGGGCAATGG AGCGCCTGCC GGCCCAGTAT CAGCCCGTCA TACTTGAAGC TAGACAGGCT

     1741 TATCTTGGAC AAGAAGAAGA TCGCTTGGCC TCGCGCGCAG ATCAGTTGGA AGAATTTGTC

     1801 CACTACGTGA AAGGCGAGAT CACCAAGGTA GTCGGCAAAT AACACTATAA GGGCGAATTC

     1861 TGCAGATATC CATCACACTG GCGGCCGCCA GTGTGATGGA TATCTGCAGA ATTCGCCCTT

     1921 CATCACTGCA GACGGGGCTT TTTTATGCAG GCAACTTTGT ATAATAAAGT TGAACGAGAA

     1981 ACGTAAAATG ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA

     2041 TAATACTGTA AAACACAACA TATCCAGTCA CTATGAATCA ACTACTTAGA TGGTATTAGT

     2101 GACCTGTACT GCAGGGCGGC CGCGATATCC CCTATAGTGA GTCGTATTAC ATGGTCATAG

     2161 CTGTTTCCTG GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA

     2221 AAAATATATC ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG

     2281 TTATGAGCCA TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG

     2341 ATTTATATGG GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC

     2401 GCTTGTATGG GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG

     2461 CCAATGATGT TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC

     2521 CGACCATCAA GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC

     2581 CCGGAAAAAC AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG

     2641 ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA

     2701 ACAGCGATCG CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG

     2761 ATGCGAGTGA TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA

     2821 TGCATAAACT TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG

     2881 ATAACCTTAT TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA

     2941 TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT

     3001 CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC

     3061 AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT

     3121 GGCAGAGCAT TACGCTGACT TGACGGGACG GCGCAAGCTC ATGACCAAAA TCCCTTAACG

     3181 TGAGTTACGC GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT

     3241 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG

     3301 CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA

     3361 GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA

     3421 AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG

     3481 CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG

     3541 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT

     3601 ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA

     3661 GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC

     3721 TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG

     3781 AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG

     3841 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTT

//

 

pENTR-R4-pLac-Spec-R3其他相关Gateway载体:

pLKO-DEST-puro

pcDNA6.2/V5-PL-DEST

pLenti7.3-V5-DEST

pcDNA6.2/N-YFP-DEST

pLenti6-UbC-V5-DEST

pcDNA3.2/V5-DEST

pLenti4-V5-DEST

pAd/BLOCK-iT-DEST

pLenti4-BLOCK-iT     -DEST

pT-Rex-DEST30

pIB-V5-His-DEST

pLKO-DEST-EGFP

pET-DEST42

plenti6/V5-Dest

pENTR-MCS1-IRES2-EGFP

pLenti6-tdTomato-V5-DEST

pENTR-L5-pLac-Spec-L2

pLenti6/BLOCK-iT     -DEST

pENTR-GUS

pLenti6.4/R4R2/V5-DEST

pENTR-DsRed2-MCS1     miR

pLenti6.2/V5-DEST

pENTR221

pENTR-L1-pLac-lacZa-R5

pENTR3C-Dual

pENTR11-Dual

pENTR     H1 TO

pEXP4-DEST

pDONR222

pEXP3-DEST

pDONR221-P4RP3R

pENTR5'/EF1αp

pDONR     Zeo

pEXP2-DEST

pDest40

pENTR4-FLAG

pDEST24

pENTR4

pDEST22

pENTR2B

pDEST10

pEXP1-DEST

pcDNA-DEST53

pDONR-P2RP3

pcDNA6.2-N-EmGFP-DEST

pDONR221-P1P4

pAd-BLOCK-iT-DEST

pDONR221

pLKO-DEST-YFP

pDEST26

pLenti6.2/C-Lumio/V5-DEST

pDEST15

pLenti6/TR

pcDNA-DEST40

pLenti4/TO/V5-DEST

pcDNA6/BioEase-DEST

pLenti4/V5-GW/LacZ

pcDNA6.2/GW/EmGFP-miR     negative

pENTR-MCS1-IRES2-DsRed2

pcDNA6.2/C-EmGFP-DEST

pENTR-L5-LacI-L4

pAd/CMV/V5-DEST

pENTR-L1pLaclacZaL4

pT-Rex-DEST31

pENTR11-Dual

pMT-DEST48

pENTR5'/CMVp

pLKO-DEST-hygro

pENTR4-Dual

plenti-CMV-puro-DEST

pENTR1A

pLenti6-capTEV-CT-DEST

pEF-DEST51

pLenti6.3-V5-DEST

pDONR223

pENTR-R4-pLac-Spec-R3

pDONR221-P5P4

pENTR-GeneBLAzer

pDONR221-P5P2

pENTR5-UbCp

pDONR201

pENTR3C

pDEST20

pENTR     U6

pDEST17

pENTR     2B Dual

pDEST12.2

pEBNA-DEST

pDONR-P4P1R

pET301/CT-DEST

pDONR207

pET300/NT-DEST

pDEST27

pcDNA6.2/cGeneBLAzer-DEST

pDEST14

pCMV-SPORT6

pDEST8

pCMV-SPORT6

pcDNA-DEST47

pcDNA6.2/nGeneBLAzer-DEST

pcDNA6.2-V5-DEST

pcDNA6.2/C-YFP-DEST

pcDNA3.1-nV5-DEST

pcDNA6.2/cTC-Tag-DEST

pBAD-DEST49

pcDNA6.2/cLumio-DEST

pAd-PL-DEST

pcDNA6.2/nLumio-DEST

pMT/BioEase-DEST

pDEST32

pXINSECT-DEST38

pYES-DEST52

pXINSECT-DEST39