北京华越洋生物
13581845453(微信)
7*24h全国热线:
15011481284
首页    载体及质粒    pDONR221-P1P4
pDONR221-P1P4Map

pDONR221-P1P4

pDONR221-P1P4

pDONR221-P1P4

 



编号

载体名称

北京华越洋VECT511199

pDONR221-P1P4

 

pDONR221-P1P4载体基本信息:

载体名称:

pDONR221-P1P4 pDONR221 P1-P4

质粒类型:

Mltisite-Gateway载体;DONR载体

高拷贝/低拷贝:

低拷贝

启动子:

克隆方法:

GatewayBP反应

载体大小:

4774bp

5' 测序引物及序列:

M13/pUC fwd   5'CCCAGTCACGACGTTGTAAAACG 3'

3' 测序引物及序列:

M13/pUC rev 5'   AGCGGATAACAATTTCACACAGG 3'

载体标签:

载体抗性:

卡那霉素,氯霉素(仅空载体)

真核筛选标记:

克隆菌株:

阳性筛选用DB3.1,阴性筛选用OmniMAX2-T1

备注:

 

pDONR221-P1P4载体是MultiSite Gateway载体,
 
是研究启动子的理想工具。
   

病毒/非病毒:

非病毒

 

pDONR221-P1P4载体质粒图谱和多克隆位点信息:

  

 

pDONR221-P1P4载体简介:

 

pDONR221-P1P4载体序列:

ORIGIN

        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA

       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA

      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA

      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC

      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA

      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC

      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA

      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG

      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA

      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC

      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA

      661 AGCTGAACGA GAAACGTAAA ATGATATAAA TATCAATATA TTAAATTAGA TTTTGCATAA

      721 AAAACAGACT ACATAATACT GTAAAACACA ACATATCCAG TCACTATGAA TCAACTACTT

      781 AGATGGTATT AGTGACCTGT AGTCGACCGA CAGCCTTCCA AATGTTCTTC GGGTGATGCT

      841 GCCAACTTAG TCGACCGACA GCCTTCCAAA TGTTCTTCTC AAACGGAATC GTCGTATCCA

      901 GCCTACTCGC TATTGTCCTC AATGCCGTAT TAAATCATAA AAAGAAATAA GAAAAAGAGG

      961 TGCGAGCCTC TTTTTTGTGT GACAAAATAA AAACATCTAC CTATTCATAT ACGCTAGTGT

     1021 CATAGTCCTG AAAATCATCT GCATCAAGAA CAATTTCACA ACTCTTATAC TTTTCTCTTA

     1081 CAAGTCGTTC GGCTTCATCT GGATTTTCAG CCTCTATACT TACTAAACGT GATAAAGTTT

     1141 CTGTAATTTC TACTGTATCG ACCTGCAGAC TGGCTGTGTA TAAGGGAGCC TGACATTTAT

     1201 ATTCCCCAGA ACATCAGGTT AATGGCGTTT TTGATGTCAT TTTCGCGGTG GCTGAGATCA

     1261 GCCACTTCTT CCCCGATAAC GGAGACCGGC ACACTGGCCA TATCGGTGGT CATCATGCGC

     1321 CAGCTTTCAT CCCCGATATG CACCACCGGG TAAAGTTCAC GGGAGACTTT ATCTGACAGC

     1381 AGACGTGCAC TGGCCAGGGG GATCACCATC CGTCGCCCGG GCGTGTCAAT AATATCACTC

     1441 TGTACATCCA CAAACAGACG ATAACGGCTC TCTCTTTTAT AGGTGTAAAC CTTAAACTGC

     1501 ATTTCACCAG CCCCTGTTCT CGTCAGCAAA AGAGCCGTTC ATTTCAATAA ACCGGGCGAC

     1561 CTCAGCCATC CCTTCCTGAT TTTCCGCTTT CCAGCGTTCG GCACGCAGAC GACGGGCTTC

     1621 ATTCTGCATG GTTGTGCTTA CCAGACCGGA GATATTGACA TCATATATGC CTTGAGCAAC

     1681 TGATAGCTGT CGCTGTCAAC TGTCACTGTA ATACGCTGCT TCATAGCATA CCTCTTTTTG

     1741 ACATACTTCG GGTATACATA TCAGTATATA TTCTTATACC GCAAAAATCA GCGCGCAAAT

     1801 ACGCATACTG TTATCTGGCT TTTAGTAAGC CGGATCCACG CGGCGTTTAC GCCCCCCCTG

     1861 CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA

     1921 AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA

     1981 TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA

     2041 ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT

     2101 AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA

     2161 CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG

     2221 GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC

     2281 CATACGGAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA

     2341 AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT

     2401 CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA

     2461 TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG CTTCCTTAGC

     2521 TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT CATTATGGTG

     2581 AAAGTTGGAA CCTCTTACGT GCCGATCAAC GTCTCATTTT CGCCAAAAGT TGGCCCAGGG

     2641 CTTCCCGGTA TCAACAGGGA CACCAGGATT TATTTATTCT GCGAAGTGAT CTTCCGTCAC

     2701 AGGTATTTAT TCGGCGCAAA GTGCGTCGGG TGATGCTGCC AACTTAGTCG ACTACAGGTC

     2761 ACTAATACCA TCTAAGTAGT TGATTCATAG TGACTGGATA TGTTGTGTTT TACAGTATTA

     2821 TGTAGTCTGT TTTTTATGCA AAATCTAATT TAATATATTG ATATTTATAT CATTTTACGT

     2881 TTCTCGTTCA ACTTTTCTAT ACAAAGTTGG CATTATAAGA AAGCATTGCT TATCAATTTG

     2941 TTGCAACGAA CAGGTCACTA TCAGTCAAAA TAAAATCATT ATTTGCCATC CAGCTGCAGG

     3001 GCGGCCGCGA TATCCCCTAT AGTGAGTCGT ATTACATGGT CATAGCTGTT TCCTGGCAGC

     3061 TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAAAT ATATCATCAT

     3121 GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC

     3181 AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA

     3241 AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGCTTG TATGGGAAGC

     3301 CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG

     3361 ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT

     3421 TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGA AAAACAGCAT

     3481 TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT

     3541 TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT

     3601 TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG

     3661 ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTTTTGC

     3721 CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG

     3781 ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC

     3841 AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC

     3901 TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC

     3961 TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA ACACTGGCAG AGCATTACGC

     4021 TGACTTGACG GGACGGCGCA AGCTCATGAC CAAAATCCCT TAACGTGAGT TACGCGTCGT

     4081 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC

     4141 TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC

     4201 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC

     4261 CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC

     4321 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT

     4381 CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT

     4441 GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT

     4501 ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT

     4561 ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG

     4621 CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT

     4681 GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT

     4741 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTT

//

 

pDONR221-P1P4其他相关Gateway载体:

pLKO-DEST-puro

pcDNA6.2/V5-PL-DEST

pLenti7.3-V5-DEST

pcDNA6.2/N-YFP-DEST

pLenti6-UbC-V5-DEST

pcDNA3.2/V5-DEST

pLenti4-V5-DEST

pAd/BLOCK-iT-DEST

pLenti4-BLOCK-iT     -DEST

pT-Rex-DEST30

pIB-V5-His-DEST

pLKO-DEST-EGFP

pET-DEST42

plenti6/V5-Dest

pENTR-MCS1-IRES2-EGFP

pLenti6-tdTomato-V5-DEST

pENTR-L5-pLac-Spec-L2

pLenti6/BLOCK-iT     -DEST

pENTR-GUS

pLenti6.4/R4R2/V5-DEST

pENTR-DsRed2-MCS1     miR

pLenti6.2/V5-DEST

pENTR221

pENTR-L1-pLac-lacZa-R5

pENTR3C-Dual

pENTR11-Dual

pENTR     H1 TO

pEXP4-DEST

pDONR222

pEXP3-DEST

pDONR221-P4RP3R

pENTR5'/EF1αp

pDONR     Zeo

pEXP2-DEST

pDest40

pENTR4-FLAG

pDEST24

pENTR4

pDEST22

pENTR2B

pDEST10

pEXP1-DEST

pcDNA-DEST53

pDONR-P2RP3

pcDNA6.2-N-EmGFP-DEST

pDONR221-P1P4

pAd-BLOCK-iT-DEST

pDONR221

pLKO-DEST-YFP

pDEST26

pLenti6.2/C-Lumio/V5-DEST

pDEST15

pLenti6/TR

pcDNA-DEST40

pLenti4/TO/V5-DEST

pcDNA6/BioEase-DEST

pLenti4/V5-GW/LacZ

pcDNA6.2/GW/EmGFP-miR     negative

pENTR-MCS1-IRES2-DsRed2

pcDNA6.2/C-EmGFP-DEST

pENTR-L5-LacI-L4

pAd/CMV/V5-DEST

pENTR-L1pLaclacZaL4

pT-Rex-DEST31

pENTR11-Dual

pMT-DEST48

pENTR5'/CMVp

pLKO-DEST-hygro

pENTR4-Dual

plenti-CMV-puro-DEST

pENTR1A

pLenti6-capTEV-CT-DEST

pEF-DEST51

pLenti6.3-V5-DEST

pDONR223

pENTR-R4-pLac-Spec-R3

pDONR221-P5P4

pENTR-GeneBLAzer

pDONR221-P5P2

pENTR5-UbCp

pDONR201

pENTR3C

pDEST20

pENTR     U6

pDEST17

pENTR     2B Dual

pDEST12.2

pEBNA-DEST

pDONR-P4P1R

pET301/CT-DEST

pDONR207

pET300/NT-DEST

pDEST27

pcDNA6.2/cGeneBLAzer-DEST

pDEST14

pCMV-SPORT6

pDEST8

pCMV-SPORT6

pcDNA-DEST47

pcDNA6.2/nGeneBLAzer-DEST

pcDNA6.2-V5-DEST

pcDNA6.2/C-YFP-DEST

pcDNA3.1-nV5-DEST

pcDNA6.2/cTC-Tag-DEST

pBAD-DEST49

pcDNA6.2/cLumio-DEST

pAd-PL-DEST

pcDNA6.2/nLumio-DEST

pMT/BioEase-DEST

pDEST32

pXINSECT-DEST38

pYES-DEST52

pXINSECT-DEST39