北京华越洋生物
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首页    载体及质粒    pDONR207
pDONR207载体图谱

pDONR207

pDONR207

pDONR207

 



编号

载体名称

北京华越洋VECT511177

pDONR207

 

pDONR207  Gateway载体基本信息:

载体名称:

pDONR207

质粒类型:

Gateway系统的DONR载体

高拷贝/低拷贝:

低拷贝

克隆方法:

Gateway

载体大小:

5585

5' 测序引物及序列:

pDONR-F: 5'-TCGCGTTAACGCTAGCATGGATCTC-3'

3' 测序引物及序列:

pDONR-R:   5'-GTAACATCAGAGATTTTGAGACAC-3'

载体标签:

--

载体抗性:

Gentamycin 2.html'   target='_blank'>庆大霉素 氯霉素

备注:


 
载体本身含有CCDb致死基因,能够使得普通的DH5alpha等细菌死亡,必须 使用DB3.1感受态细胞进行质粒扩增,用来构建Gateway系统入门载体;
  pDONR207
尽管含有pUC质粒的起始复制点,但是细菌内仍然是低产量质粒;
 
构建Gateway系统入门克隆时需要使用ccdB敏感的 OmniMAX2感受态细胞。
   

病毒/非病毒:

非病毒

 

pDONR207载体质粒图谱和多克隆位点信息:

  

 

pDONR207载体简介

pDONR207载体序列:

ORIGIN

    1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCC AGGAAGAGTT

   61 TGTAGAAACG CAAAAAGGCC ATCCGTCAGG ATGGCCTTCT GCTTAGTTTG ATGCCTGGCA

  121 GTTTATGGCG GGCGTCCTGC CCGCCACCCT CCGGGCCGTT GCTTCACAAC GTTCAAATCC

  181 GCTCCCGGCG GATTTGTCCT ACTCAGGAGA GCGTTCACCG ACAAACAACA GATAAAACGA

  241 AAGGCCCAGT CTTCCGACTG AGCCTTTCGT TTTATTTGAT GCCTGGCAGT TCCCTACTCT

  301 CGCGTTAACG CTAGCATGGA TCTCGGGCCC CAAATAATGA TTTTATTTTG ACTGATAGTG

  361 ACCTGTTCGT TGCAACAAAT TGATGAGCAA TGCTTTTTTA TAATGCCAAG TTTGTACAAA

  421 AAAGCAGAAC GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA GATTTTGCAT

  481 AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC AGTCACTATG AATCAACTAC

  541 TTAGATGGTA TTAGTGACCT GTAGTCGACC GACAGCCTTC CAAATGTTCT TCGGGTGATG

  601 CTGCCAACTT AGTCGACCGA CAGCCTTCCA AATGTTCTTC TCAAACGGAA TCGTCGTATC

  661 CAGCCTACTC GCTATTGTCC TCAATGCCGT ATTAAATCAT AAAAAGAAAT AAGAAAAAGA

  721 GGTGCGAGCC TCTTTTTTGT GTGACAAAAT AAAAACATCT ACCTATTCAT ATACGCTAGT

  781 GTCATAGTCC TGAAAATCAT CTGCATCAAG AACAATTTCA CAACTCTTAT ACTTTTCTCT

  841 TACAAGTCGT TCGGCTTCAT CTGGATTTTC AGCCTCTATA CTTACTAAAC GTGATAAAGT

  901 TTCTGTAATT TCTACTGTAT CGACCTGCAG ACTGGCTGTG TATAAGGGAG CCTGACATTT

  961 ATATTCCCCA GAACATCAGG TTAATGGCGT TTTTGATGTC ATTTTCGCGG TGGCTGAGAT

 1021 CAGCCACTTC TTCCCCGATA ACGGAGACCG GCACACTGGC CATATCGGTG GTCATCATGC

 1081 GCCAGCTTTC ATCCCCGATA TGCACCACCG GGTAAAGTTC ACGGGAGACT TTATCTGACA

 1141 GCAGACGTGC ACTGGCCAGG GGGATCACCA TCCGTCGCCC GGGCGTGTCA ATAATATCAC

 1201 TCTGTACATC CACAAACAGA CGATAACGGC TCTCTCTTTT ATAGGTGTAA ACCTTAAACT

 1261 GCATTTCACC AGTCCCTGTT CTCGTCAGCA AAAGAGCCGT TCATTTCAAT AAACCGGGCG

 1321 ACCTCAGCCA TCCCTTCCTG ATTTTCCGCT TTCCAGCGTT CGGCACGCAG ACGACGGGCT

 1381 TCATTCTGCA TGGTTGTGCT TACCAGACCG GAGATATTGA CATCATATAT GCCTTGAGCA

 1441 ACTGATAGCT GTCGCTGTCA ACTGTCACTG TAATACGCTG CTTCATAGCA CACCTCTTTT

 1501 TGACATACTT CGGGTATACA TATCAGTATA TATTCTTATA CCGCAAAAAT CAGCGCGCAA

 1561 ATACGCATAC TGTTATCTGG CTTTTAGTAA GCCGGATCCA CGCGATTACG CCCCGCCCTG

 1621 CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA

 1681 GACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA

 1741 TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA

 1801 ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT

 1861 AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA

 1921 CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG

 1981 GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC

 2041 CATACGGAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA

 2101 AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT

 2161 CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA

 2221 TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG CTTCCTTAGC

 2281 TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT CATTATGGTG

 2341 AAAGTTGGAA CCTCTTACGT GCCGATCAAC GTCTCATTTT CGCCAAAAGT TGGCCCAGGG

 2401 CTTCCCGGTA TCAACAGGGA CACCAGGATT TATTTATTCT GCGAAGTGAT CTTCCGTCAC

 2461 AGGTATTTAT TCGGCGCAAA GTGCGTCGGG TGATGCTGCC AACTTAGTCG ACTACAGGTC

 2521 ACTAATACCA TCTAAGTAGT TGATTCATAG TGACTGGATA TGTTGTGTTT TACAGTATTA

 2581 TGTAGTCTGT TTTTTATGCA AAATCTAATT TAATATATTG ATATTTATAT CATTTTACGT

 2641 TTCTCGTTCA GCTTTCTTGT ACAAAGTGGG CATTATAAGA AAGCATTGCT TATCAATTTG

 2701 TTGCAACGAA CAGGTCACTA TCAGTCAAAA TAAAATCATT ATTTGCCATC CAGCTGCAGC

 2761 TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAAAT ATATCATCAT

 2821 GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC

 2881 AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA

 2941 AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGCTTG TATGGGAAGC

 3001 CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG

 3061 ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT

 3121 TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGA AAAACAGCAT

 3181 TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT

 3241 TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT

 3301 TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG

 3361 ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTTTTGC

 3421 CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG

 3481 ACGAGGGGAA ATTAGGCCGG GAAGCCGATC TCGGCTTGAA CGAATTGTTA GGTGGCGGTA

 3541 CTTGGGTCGA TATCAAAGTG CATCACTTCT TCCCGTATGC CCAACTTTGT ATAGAGAGCC

 3601 ACTGCGGGAT CGTCACCGTA ATCTGCTTGC ACGTAGATCA CATAAGCACC AAGCGCGTTG

 3661 GCCTCATGCT TGAGGAGATT GATGAGCGCG GTGGCAATGC CCTGCCTCCG GTGCTCGCCG

 3721 GAGACTGCGA GATCATAGAT ATAGATCTCA CTACGCGGCT GCTCAAACCT GGGCAGAACG

 3781 TAAGCCGCGA GAGCGCCAAC AACCGCTTCT TGGTCGAAGG CAGCAAGCGC GATGAATGTC

 3841 TTACTACGGA GCAAGTTCCC GAGGTAATCG GAGTCCGGCT GATGTTGGGA GTAGGTGGCT

 3901 ACGTCTCCGA ACTCACGACC GAAAAGATCA AGAGCAGCCC GCATGGATTT GACTTGGTCA

 3961 GGGCCGAGCC TACATGTGCG AATGATGCCC ATACTTGAGC CACCTAACTT TGTTTTAGGG

 4021 CGACTGCCCT GCTGCGTAAC ATCGTTGCTG CTGCGTAACA TCGTTGCTGC TCCATAACAT

 4081 CAAACATCGA CCCACGGCGT AACGCGCTTG CTGCTTGGAT GCCCGAGGCA TAGACTGTAC

 4141 AAAAAAACAG TCATAACAAG CCATGAAAAC CGCCACTGCG CCGTTACCAC CGCTGCGTTC

 4201 GGTCAAGGTT CTGGACCAGT TGCGTGAGCG CATACGCTAC TTGCATTACA GTTTACGAAC

 4261 CGAACAGGCT TATGTCAACT GGGTTCGTGC CTTCATCCGT TTCCACGGTG TGCGTCACCC

 4321 GGCAACCTTG GGCAGCAGCG AAGTCGAGGC ATTTCTGTCC TGGCTGGCGA ACGAGCGCAA

 4381 GGTTTCGGTC TCCACGCATC GTCAGGCATT GGCGGCCTTG CTGTTCTTCT ACGGCAAGGT

 4441 GCTGTGCACG GATCTGCCCT GGCTTCAGGA GATCGGAAGA CCTCGGCCGT CGCGGCGCTT

 4501 GCCGGTGGTG CTGACCCCGG ATGAAGTGGT TCGCATCCTC GGTTTTCTGG AAGGCGAGCA

 4561 TCGTTTGTTC GCCCAGGACT CTAGCTATAG TTCTAGTGGT TGGCTACTAA TAGGTTGTAT

 4621 TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG

 4681 CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA

 4741 TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT

 4801 GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG GCGCAAGCTC

 4861 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG

 4921 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA

 4981 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG

 5041 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG

 5101 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG

 5161 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA

 5221 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC

 5281 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC

 5341 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA

 5401 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT

 5461 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG

 5521 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC

 5581 ATGTT

//

 

pDONR207其他相关Gateway载体:

pLKO-DEST-puro

pcDNA6.2/V5-PL-DEST

pLenti7.3-V5-DEST

pcDNA6.2/N-YFP-DEST

pLenti6-UbC-V5-DEST

pcDNA3.2/V5-DEST

pLenti4-V5-DEST

pAd/BLOCK-iT-DEST

pLenti4-BLOCK-iT     -DEST

pT-Rex-DEST30

pIB-V5-His-DEST

pLKO-DEST-EGFP

pET-DEST42

plenti6/V5-Dest

pENTR-MCS1-IRES2-EGFP

pLenti6-tdTomato-V5-DEST

pENTR-L5-pLac-Spec-L2

pLenti6/BLOCK-iT     -DEST

pENTR-GUS

pLenti6.4/R4R2/V5-DEST

pENTR-DsRed2-MCS1     miR

pLenti6.2/V5-DEST

pENTR221

pENTR-L1-pLac-lacZa-R5

pENTR3C-Dual

pENTR11-Dual

pENTR     H1 TO

pEXP4-DEST

pDONR222

pEXP3-DEST

pDONR221-P4RP3R

pENTR5'/EF1αp

pDONR     Zeo

pEXP2-DEST

pDest40

pENTR4-FLAG

pDEST24

pENTR4

pDEST22

pENTR2B

pDEST10

pEXP1-DEST

pcDNA-DEST53

pDONR-P2RP3

pcDNA6.2-N-EmGFP-DEST

pDONR221-P1P4

pAd-BLOCK-iT-DEST

pDONR221

pLKO-DEST-YFP

pDEST26

pLenti6.2/C-Lumio/V5-DEST

pDEST15

pLenti6/TR

pcDNA-DEST40

pLenti4/TO/V5-DEST

pcDNA6/BioEase-DEST

pLenti4/V5-GW/LacZ

pcDNA6.2/GW/EmGFP-miR     negative

pENTR-MCS1-IRES2-DsRed2

pcDNA6.2/C-EmGFP-DEST

pENTR-L5-LacI-L4

pAd/CMV/V5-DEST

pENTR-L1pLaclacZaL4

pT-Rex-DEST31

pENTR11-Dual

pMT-DEST48

pENTR5'/CMVp

pLKO-DEST-hygro

pENTR4-Dual

plenti-CMV-puro-DEST

pENTR1A

pLenti6-capTEV-CT-DEST

pEF-DEST51

pLenti6.3-V5-DEST

pDONR223

pENTR-R4-pLac-Spec-R3

pDONR221-P5P4

pENTR-GeneBLAzer

pDONR221-P5P2

pENTR5-UbCp

pDONR201

pENTR3C

pDEST20

pENTR     U6

pDEST17

pENTR     2B Dual

pDEST12.2

pEBNA-DEST

pDONR-P4P1R

pET301/CT-DEST

pDONR207

pET300/NT-DEST

pDEST27

pcDNA6.2/cGeneBLAzer-DEST

pDEST14

pCMV-SPORT6

pDEST8

pCMV-SPORT6

pcDNA-DEST47

pcDNA6.2/nGeneBLAzer-DEST

pcDNA6.2-V5-DEST

pcDNA6.2/C-YFP-DEST

pcDNA3.1-nV5-DEST

pcDNA6.2/cTC-Tag-DEST

pBAD-DEST49

pcDNA6.2/cLumio-DEST

pAd-PL-DEST

pcDNA6.2/nLumio-DEST

pMT/BioEase-DEST

pDEST32

pXINSECT-DEST38

pYES-DEST52

pXINSECT-DEST39