北京华越洋生物
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首页    载体及质粒    pENTR-L5-pLac-Spec-L2
pENTR-L5-pLac-Spec-L2Map

pENTR-L5-pLac-Spec-L2

pENTR-L5-pLac-Spec-L2

pENTR-L5-pLac-Spec-L2

 



编号

载体名称

北京华越洋VECT511126

pENTR-L5-pLac-Spec-L2

 

pENTR-L5-pLac-Spec-L2载体基本信息:

载体名称:

pENTR-L5-pLac-Spec-L2

质粒类型:

多位点Gateway载体;入门载体

高拷贝/低拷贝:

高拷贝

启动子:

克隆方法:

限制性内切酶;多克隆位点

载体大小:

3713bp

5' 测序引物及序列:

pENTR-F:   CTACAAACTCTTCCTGTTAGTTAG

3' 测序引物及序列:

pENTR-R:   ATGGCTCATAACACCCCTTG

载体标签:

载体抗性:

卡那霉素

真核筛选标记:

克隆菌株:

DH5aTOP10感受态细胞

病毒/非病毒:

非病毒

 

pENTR-L5-pLac-Spec-L2载体质粒图谱和多克隆位点信息:

pENTR-L5-pLac-Spec-L2载体简介

pENTR-L5-pLac-Spec-L2载体序列:

ORIGIN

        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA

       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA

      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA

      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC

      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA

      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC

      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA

      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG

      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA

      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC

      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTATACAAA

      661 AGTTGGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT

      721 ATTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG CATCGCTCAC

      781 GCAACTGGTC CAGAACCTTG ACCGAACGCA GCGGTGGTAA CGGCGCAGTG GCGGTTTTCA

      841 TGGCTTGTTA TGACTGTTTT TTTGGGGTAC AGTCTATGCC TCGGGCATCC AAGCAGCAAG

      901 CGCGTTACGC CGTGGGTCGA TGTTTGATGT TATGGAGCAG CAACGATGTT ACGCAGCAGG

      961 GCAGTCGCCC TAAAACAAAG TTAAACATTA TGAGGGAAGC GGTGATCGCC GAAGTATCGA

     1021 CTCAACTATC AGAGGTAGTT GGCGTCATCG AGCGCCATCT CGAACCGACG TTGCTGGCCG

     1081 TACATTTGTA CGGCTCCGCA GTGGATGGCG GCCTGAAGCC ACACAGTGAT ATTGATTTGC

     1141 TGGTTACGGT GACCGCAAGG CTTGATGAAA CAACGCGGCG AGCTTTGATC GACGACCTTT

     1201 TGGAAACTTC GGCTTCCCCT GGAGAGAGCG AGATTCTCCG CGCTGTAGAA GTCACCATTG

     1261 TTGTGCACGA CGACATCATT CCGTGGCGTT ATCCAGCTAA GCGCGAACTG CAATTTGGAG

     1321 AATGGCAGCG CAATGACATT CTTGCAGGTA TCTTCGAGCC AGCCACGATC GACATTGATC

     1381 TGGCTATCTT GCTGACAAAA GCAAGAGAAC ATAGCGTTGC CTTGGTAGGT CCAGCGGCGG

     1441 AGGAACTCTT TGATCCGGTT CCTGAACAGG ATCTATTTGA GGCGCTAAAT GAAACCTTAA

     1501 CGCTATGGAA CTCGCCGCCC GACTGGGCTG GCGATGAGCG AAATGTAGTG CTTACGTTGT

     1561 CCCGCATTTG GTACAGCGCA GTAACCGGCA AAATCGCGCC GAAGGATGTC GCTGCCGACT

     1621 GGGCAATGGA GCGCCTGCCG GCCCAGTATC AGCCCGTCAT ACTTGAAGCT AGACAGGCTT

     1681 ATCTTGGACA AGAAGAAGAT CGCTTGGCCT CGCGCGCAGA TCAGTTGGAA GAATTTGTCC

     1741 ACTACGTGAA AGGCGAGATC ACCAAGGTAG TCGGCAAATA ACACTATAAG GGCGGATGAT

     1801 GCAGACGGGG CTTTTTTATG CAGGACCCAG CTTTCTTGTA CAAAGTTGGC ATTATAAGAA

     1861 AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA

     1921 TTTGCCATCC AGCTGCAGGG CGGCCGCGAT ATCCCCTATA GTGAGTCGTA TTACATGGTC

     1981 ATAGCTGTTT CCTGGCAGCT CTGGCCCGTG TCTCAAAATC TCTGATGTTA CATTGCACAA

     2041 GATAAAAATA TATCATCATG AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG

     2101 GGTGTTATGA GCCATATTCA ACGGGAAACG TCGAGGCCGC GATTAAATTC CAACATGGAT

     2161 GCTGATTTAT ATGGGTATAA ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC

     2221 TATCGCTTGT ATGGGAAGCC CGATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC

     2281 GTTGCCAATG ATGTTACAGA TGAGATGGTC AGACTAAACT GGCTGACGGA ATTTATGCCT

     2341 CTTCCGACCA TCAAGCATTT TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG

     2401 ATCCCCGGAA AAACAGCATT CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT

     2461 GTTGATGCGC TGGCAGTGTT CCTGCGCCGG TTGCATTCGA TTCCTGTTTG TAATTGTCCT

     2521 TTTAACAGCG ATCGCGTATT TCGTCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG

     2581 GTTGATGCGA GTGATTTTGA TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA

     2641 GAAATGCATA AACTTTTGCC ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA

     2701 CTTGATAACC TTATTTTTGA CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC

     2761 GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT

     2821 CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA

     2881 TTGCAGTTTC ATTTGATGCT CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA

     2941 CACTGGCAGA GCATTACGCT GACTTGACGG GACGGCGCAA GCTCATGACC AAAATCCCTT

     3001 AACGTGAGTT ACGCGTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT

     3061 TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA

     3121 CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC

     3181 TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC

     3241 TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT

     3301 GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT

     3361 AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG

     3421 ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA

     3481 GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG

     3541 GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA

     3601 CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC

     3661 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTT

//

 

pENTR-L5-pLac-Spec-L2其他相关Gateway载体:

pLKO-DEST-puro

pcDNA6.2/V5-PL-DEST

pLenti7.3-V5-DEST

pcDNA6.2/N-YFP-DEST

pLenti6-UbC-V5-DEST

pcDNA3.2/V5-DEST

pLenti4-V5-DEST

pAd/BLOCK-iT-DEST

pLenti4-BLOCK-iT     -DEST

pT-Rex-DEST30

pIB-V5-His-DEST

pLKO-DEST-EGFP

pET-DEST42

plenti6/V5-Dest

pENTR-MCS1-IRES2-EGFP

pLenti6-tdTomato-V5-DEST

pENTR-L5-pLac-Spec-L2

pLenti6/BLOCK-iT     -DEST

pENTR-GUS

pLenti6.4/R4R2/V5-DEST

pENTR-DsRed2-MCS1     miR

pLenti6.2/V5-DEST

pENTR221

pENTR-L1-pLac-lacZa-R5

pENTR3C-Dual

pENTR11-Dual

pENTR     H1 TO

pEXP4-DEST

pDONR222

pEXP3-DEST

pDONR221-P4RP3R

pENTR5'/EF1αp

pDONR     Zeo

pEXP2-DEST

pDest40

pENTR4-FLAG

pDEST24

pENTR4

pDEST22

pENTR2B

pDEST10

pEXP1-DEST

pcDNA-DEST53

pDONR-P2RP3

pcDNA6.2-N-EmGFP-DEST

pDONR221-P1P4

pAd-BLOCK-iT-DEST

pDONR221

pLKO-DEST-YFP

pDEST26

pLenti6.2/C-Lumio/V5-DEST

pDEST15

pLenti6/TR

pcDNA-DEST40

pLenti4/TO/V5-DEST

pcDNA6/BioEase-DEST

pLenti4/V5-GW/LacZ

pcDNA6.2/GW/EmGFP-miR     negative

pENTR-MCS1-IRES2-DsRed2

pcDNA6.2/C-EmGFP-DEST

pENTR-L5-LacI-L4

pAd/CMV/V5-DEST

pENTR-L1pLaclacZaL4

pT-Rex-DEST31

pENTR11-Dual

pMT-DEST48

pENTR5'/CMVp

pLKO-DEST-hygro

pENTR4-Dual

plenti-CMV-puro-DEST

pENTR1A

pLenti6-capTEV-CT-DEST

pEF-DEST51

pLenti6.3-V5-DEST

pDONR223

pENTR-R4-pLac-Spec-R3

pDONR221-P5P4

pENTR-GeneBLAzer

pDONR221-P5P2

pENTR5-UbCp

pDONR201

pENTR3C

pDEST20

pENTR     U6

pDEST17

pENTR     2B Dual

pDEST12.2

pEBNA-DEST

pDONR-P4P1R

pET301/CT-DEST

pDONR207

pET300/NT-DEST

pDEST27

pcDNA6.2/cGeneBLAzer-DEST

pDEST14

pCMV-SPORT6

pDEST8

pCMV-SPORT6

pcDNA-DEST47

pcDNA6.2/nGeneBLAzer-DEST

pcDNA6.2-V5-DEST

pcDNA6.2/C-YFP-DEST

pcDNA3.1-nV5-DEST

pcDNA6.2/cTC-Tag-DEST

pBAD-DEST49

pcDNA6.2/cLumio-DEST

pAd-PL-DEST

pcDNA6.2/nLumio-DEST

pMT/BioEase-DEST

pDEST32

pXINSECT-DEST38

pYES-DEST52

pXINSECT-DEST39