pCI-NEO

pCI-NEO

编号	载体名称
北京华越洋VECT90204	pCI-NEO

pCI-NEO载体基本信息：

启动子:	CMV IE
复制子:	pUC ori，F1 ori
终止子:	SV40 poly（A） signal
质粒分类:	哺乳细胞载体;蛋白过表达质粒
质粒大小:	5472bp
原核抗性:	氨苄青霉素Amp
筛选标记:	新霉素Neo
克隆菌株:	DH5α
培养条件:	37℃，有氧，LB
表达宿主:	哺乳细胞
诱导方式:	无需诱导，瞬时表达
5'测序引物:	CMV-F: 5-CGCAAATGGGCGGTAGGCGTG-3
3'测序引物:	EBV-R:5-GTGGTTTGTCCAAACTCATC-3

pCI-NEO载体质粒图谱和多克隆位点信息:

pCI-NEO载体简介:

pCI-neo Mammalian Expression Vector(pCI-neo 哺乳动物表达载体) 携带有人类巨细胞病毒(CMV) 的即刻早期增强子/ 启动子区域，来促进哺乳动物细胞中插入克隆DNA 的组成型表达。此载体也带有新霉素磷酸转移酶基因, 一种哺乳动物细胞的选择性标志物。通过G-418 抗性筛选转染细胞，pCI-neo 载体能够用于瞬时或稳定表达。

pCI-NEO载体序列:

ORIGIN
        1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA ATATTGGCTA
       61 TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC ATTTATATTG GCTCATGTCC
      121 AATATGACCG CCATGTTGGC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG
      181 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC
      241 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT
      301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC
      361 CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG CCCCCTATTG ACGTCAATGA
      421 CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTACGGGACT TTCCTACTTG
      481 GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC
      541 CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT
      601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTG
      661 CGATCGCCCG CCCCGTTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
      721 AGCAGAGCTC GTTTAGTGAA CCGTCAGATC ACTAGAAGCT TTATTGCGGT AGTTTATCAC
      781 AGTTAAATTG CTAACGCAGT CAGTGCTTCT GACACAACAG TCTCGAACTT AAGCTGCAGT
      841 GACTCTCTTA AGGTAGCCTT GCAGAAGTTG GTCGTGAGGC ACTGGGCAGG TAAGTATCAA
      901 GGTTACAAGA CAGGTTTAAG GAGACCAATA GAAACTGGGC TTGTCGAGAC AGAGAAGACT
      961 CTTGCGTTTC TGATAGGCAC CTATTGGTCT TACTGACATC CACTTTGCCT TTCTCTCCAC
     1021 AGGTGTCCAC TCCCAGTTCA ATTACAGCTC TTAAGGCTAG AGTACTTAAT ACGACTCACT
     1081 ATAGGCTAGC CTCGAGAATT CACGCGTGGT ACCTCTAGAG TCGACCCGGG CGGCCGCTTC
     1141 CCTTTAGTGA GGGTTAATGC TTCGAGCAGA CATGATAAGA TACATTGATG AGTTTGGACA
     1201 AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC
     1261 TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT
     1321 TATGTTTCAG GTTCAGGGGG AGATGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA
     1381 ATGTGGTAAA ATCCGATAAG GATCGATCCG GGCTGGCGTA ATAGCGAAGA GGCCCGCACC
     1441 GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGACGCGCCC TGTAGCGGCG
     1501 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC
     1561 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC
     1621 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG
     1681 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG
     1741 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG
     1801 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT
     1861 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA
     1921 TATTAACGCT TACAATTTCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC
     1981 ACACCGCATA CGCGGATCTG CGCAGCACCA TGGCCTGAAA TAACCTCTGA AAGAGGAACT
     2041 TGGTTAGGTA CCTTCTGAGG CGGAAAGAAC CAGCTGTGGA ATGTGTGTCA GTTAGGGTGT
     2101 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA
     2161 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT
     2221 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG
     2281 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC
     2341 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA
     2401 GGCTTTTGCA AAAAGCTTGA TTCTTCTGAC ACAACAGTCT CGAACTTAAG GCTAGAGCCA
     2461 CCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT
     2521 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT
     2581 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC
     2641 TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG
     2701 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC
     2761 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA
     2821 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC
     2881 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG
     2941 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG
     3001 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA
     3061 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG
     3121 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT
     3181 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC
     3241 TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA
     3301 CCTGCCATCA CGATGGCCGC AATAAAATAT CTTTATTTTC ATTACATCTG TGTGTTGGTT
     3361 TTTTGTGTGA ATCGATAGCG ATAAGGATCC GCGTATGGTG CACTCTCAGT ACAATCTGCT
     3421 CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC
     3481 GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA
     3541 TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG ACGAAAGGGC CTCGTGATAC
     3601 GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT
     3661 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT
     3721 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA
     3781 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG
     3841 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC
     3901 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG
     3961 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC
     4021 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG
     4081 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT
     4141 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG
     4201 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG
     4261 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC
     4321 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT
     4381 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT
     4441 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC
     4501 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA
     4561 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT
     4621 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT
     4681 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA
     4741 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA
     4801 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC
     4861 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG
     4921 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG
     4981 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC
     5041 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT
     5101 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG
     5161 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC
     5221 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC
     5281 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC
     5341 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA
     5401 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGG
     5461 CTCGACAGAT CT
//

pCI-NEO其他相关哺乳细胞载体：

pEF1/V5-HisA	pEGFP-N3
pEF-GFP	pEGFP-C3
pFLAG-CMV2	pEGFP-N2
pCI-NEO	pEGFP-N1
pCEP4	pEGFP-1
pVAX1	pECFP-C1
pcDNA3.1/V5-His B	pECFP-N1
pCDNA6-Myc-HisB	pDsRed2-Nuc
pCDNA3.1-3×HA	pDsRed2-ER
pCDNA3.1-3×HA	Top-Flash
pCDNA3.1(-)/Myc-His B	pSTAT3-TA-luc
pCDNA3.1(-)/Myc-His A	pSilencer2.1-U6 neo
pCDNA3.1(+)/Myc-His B	psiCHECK-2
pCDNA3.1(+)-Zeo	pRL-TK
pCDNA3.1(+)-EGFP	pRL-SV40
pCDNA3.1(+)	pRL-CMV
pCDNA3.1(-)	pp53-TA-luc
pBudCE4.1-hrGFP	pNF-KB-TA-LUC
pCAGGS-HA	pNF-kB-Luc
pVAX1-FLAG	pMIR-Reporter
pSV2-DHFR	pmirGLO
pCEP4/CAT	pGL4.79[hRluc/Neo]
pCAGGS	pGL4.75[hRluc/CMV]
pCAG-GFP	pGL4.73[hRluc /SV40]
pCDNA3.0	pGL4.29[luc2P/CRE/Hygro]
pCDNA3.0-HA	pGL4.13-luci2
pCDNA3.1(+)-3×FLAG	pGL4.13-luci2
pSV-Beta-Gal	pGL4.10[luc2]
SuperCos1	pGL3-Enhancer
pT2/VENUS	pGL3-Control
pAdVAntage	pGL3-Basic
pEYFP-Tubulin	pGL3-3.6kb human IFN-gamma luc
pEGFP-C1	pENTR5'-UbCp
pmGeos-M-N1	pBiFC-VN173
pEYFP-N1	pBiFC-VC155
pEYFP-C1	pBIFC-bFosVC155
pEGFP-C2	pBiFC-bFOSDelataZipVC155/pBiFC-bFos(deltaZIP) VC155
pEYFP-Actin	Laconic/pcDNA3.1(-)
pDsRed2-Peroxi	Fop-Flash
pEBFP-C1	pTet-On-Advanced
pmGeos-M-C1	pmCherry-EGFP-LC3b-r
pCerulean-N1	pmCherry-EGFP-LC3b-m
pmKO2-N1	pmCherry-EGFP-LC3B-h
pMyosin-PSmOrange	pEGFP-LC3B-r
pTagBFP-N	pEGFP-LC3B-m
pCAG-DsRed	pEGFP-LC3B-h
pmCherry-C1	pEGFP-LC3B-c
pmCherry-N1	Super PiggyBac Transposase (PB200PA-1)
pEYFP-Golgi	pTol2-Promoter-T2A-EGFP
pEYFP-Mem	pSP64-Tol2-Transposase
pDsRed2-C1	PiggyBac Dual promoter (PB513B-1)
pEGFP-Actin	pBIND-ID
pEBFP-N1	pG5Luc
pDsRed2-N1	pFUSE-hIgG1e1-Fc2
pN3	pEE12.4
pEGFP-EcoRV	pEE6.4