pGL4.10[luc2]
pGL4.10[luc2]
编号 | 载体名称 |
北京华越洋VECT90309 | pGL4.10[luc2] |
pGL4.10[luc2]载体基本信息：
复制子: | pUC ori |
终止子: | SV40 poly(A) signal |
质粒分类: | 哺乳细胞，信号通路报告载体 |
质粒大小: | 4242bp |
原核抗性: | Amp |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧 LB |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | RVP3:CTAGCAAAATAGGCTGTCCC |
3'测序引物: | 根据序列设计引物 |
pGL4.10[luc2]载体质粒图谱和多克隆位点信息：
pGL4.10[luc2]载体简介：
The pGL4.10[luc2] Vectorencodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression.
The pGL4.10[luc2] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice.
pGL4.10[luc2]载体序列：
ORIGIN
1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC TGGCCTCGGC
61 GGCCAAGCTT GGCAATCCGG TACTGTTGGT AAAGCCACCA TGGAAGATGC CAAAAACATT
121 AAGAAGGGCC CAGCGCCATT CTACCCACTC GAAGACGGGA CCGCCGGCGA GCAGCTGCAC
181 AAAGCCATGA AGCGCTACGC CCTGGTGCCC GGCACCATCG CCTTTACCGA CGCACATATC
241 GAGGTGGACA TTACCTACGC CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG
301 AAGCGCTATG GGCTGAATAC AAACCATCGG ATCGTGGTGT GCAGCGAGAA TAGCTTGCAG
361 TTCTTCATGC CCGTGTTGGG TGCCCTGTTC ATCGGTGTGG CTGTGGCCCC AGCTAACGAC
421 ATCTACAACG AGCGCGAGCT GCTGAACAGC ATGGGCATCA GCCAGCCCAC CGTCGTATTC
481 GTGAGCAAGA AAGGGCTGCA AAAGATCCTC AACGTGCAAA AGAAGCTACC GATCATACAA
541 AAGATCATCA TCATGGATAG CAAGACCGAC TACCAGGGCT TCCAAAGCAT GTACACCTTC
601 GTGACTTCCC ATTTGCCACC CGGCTTCAAC GAGTACGACT TCGTGCCCGA GAGCTTCGAC
661 CGGGACAAAA CCATCGCCCT GATCATGAAC AGTAGTGGCA GTACCGGATT GCCCAAGGGC
721 GTAGCCCTAC CGCACCGCAC CGCTTGTGTC CGATTCAGTC ATGCCCGCGA CCCCATCTTC
781 GGCAACCAGA TCATCCCCGA CACCGCTATC CTCAGCGTGG TGCCATTTCA CCACGGCTTC
841 GGCATGTTCA CCACGCTGGG CTACTTGATC TGCGGCTTTC GGGTCGTGCT CATGTACCGC
901 TTCGAGGAGG AGCTATTCTT GCGCAGCTTG CAAGACTATA AGATTCAATC TGCCCTGCTG
961 GTGCCCACAC TATTTAGCTT CTTCGCTAAG AGCACTCTCA TCGACAAGTA CGACCTAAGC
1021 AACTTGCACG AGATCGCCAG CGGCGGGGCG CCGCTCAGCA AGGAGGTAGG TGAGGCCGTG
1081 GCCAAACGCT TCCACCTACC AGGCATCCGC CAGGGCTACG GCCTGACAGA AACAACCAGC
1141 GCCATTCTGA TCACCCCCGA AGGGGACGAC AAGCCTGGCG CAGTAGGCAA GGTGGTGCCC
1201 TTCTTCGAGG CTAAGGTGGT GGACTTGGAC ACCGGTAAGA CACTGGGTGT GAACCAGCGC
1261 GGCGAGCTGT GCGTCCGTGG CCCCATGATC ATGAGCGGCT ACGTTAACAA CCCCGAGGCT
1321 ACAAACGCTC TCATCGACAA GGACGGCTGG CTGCACAGCG GCGACATCGC CTACTGGGAC
1381 GAGGACGAGC ACTTCTTCAT CGTGGACCGG CTGAAGAGCC TGATCAAATA CAAGGGCTAC
1441 CAGGTAGCCC CAGCCGAACT GGAGAGCATC CTGCTGCAAC ACCCCAACAT CTTCGACGCC
1501 GGGGTCGCCG GCCTGCCCGA CGACGATGCC GGCGAGCTGC CCGCCGCAGT CGTCGTGCTG
1561 GAACACGGTA AAACCATGAC CGAGAAGGAG ATCGTGGACT ATGTGGCCAG CCAGGTTACA
1621 ACCGCCAAGA AGCTGCGCGG TGGTGTTGTG TTCGTGGACG AGGTGCCTAA AGGACTGACC
1681 GGCAAGTTGG ACGCCCGCAA GATCCGCGAG ATTCTCATTA AGGCCAAGAA GGGCGGCAAG
1741 ATCGCCGTGT AATAATTCTA GAGTCGGGGC GGCCGGCCGC TTCGAGCAGA CATGATAAGA
1801 TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT
1861 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC
1921 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA
1981 AGCAAGTAAA ACCTCTACAA ATGTGGTAAA ATCGATAAGG ATCCGTCGAC CGATGCCCTT
2041 GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG GGCATGACTA TCGTCGCCGC
2101 ACTTATGACT GTCTTCTTTA TCATGCAACT CGTAGGACAG GTGCCGGCAG CGCTCTTCCG
2161 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
2221 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
2281 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC
2341 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
2401 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC
2461 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG
2521 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
2581 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC
2641 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
2701 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT
2761 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG
2821 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
2881 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT
2941 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
3001 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA
3061 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGCGGCCG CAAATGCTAA ACCACTGCAG
3121 TGGTTACCAG TGCTTGATCA GTGAGGCACC GATCTCAGCG ATCTGCCTAT TTCGTTCGTC
3181 CATAGTGGCC TGACTCCCCG TCGTGTAGAT CACTACGATT CGTGAGGGCT TACCATCAGG
3241 CCCCAGCGCA GCAATGATGC CGCGAGAGCC GCGTTCACCG GCCCCCGATT TGTCAGCAAT
3301 GAACCAGCCA GCAGGGAGGG CCGAGCGAAG AAGTGGTCCT GCTACTTTGT CCGCCTCCAT
3361 CCAGTCTATG AGCTGCTGTC GTGATGCTAG AGTAAGAAGT TCGCCAGTGA GTAGTTTCCG
3421 AAGAGTTGTG GCCATTGCTA CTGGCATCGT GGTATCACGC TCGTCGTTCG GTATGGCTTC
3481 GTTCAACTCT GGTTCCCAGC GGTCAAGCCG GGTCACATGA TCACCCATAT TATGAAGAAA
3541 TGCAGTCAGC TCCTTAGGGC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CGGTGTTGTC
3601 GCTCATGGTA ATGGCAGCAC TACACAATTC TCTTACCGTC ATGCCATCCG TAAGATGCTT
3661 TTCCGTGACC GGCGAGTACT CAACCAAGTC GTTTTGTGAG TAGTGTATAC GGCGACCAAG
3721 CTGCTCTTGC CCGGCGTCTA TACGGGACAA CACCGCGCCA CATAGCAGTA CTTTGAAAGT
3781 GCTCATCATC GGGAATCGTT CTTCGGGGCG GAAAGACTCA AGGATCTTGC CGCTATTGAG
3841 ATCCAGTTCG ATATAGCCCA CTCTTGCACC CAGTTGATCT TCAGCATCTT TTACTTTCAC
3901 CAGCGTTTCG GGGTGTGCAA AAACAGGCAA GCAAAATGCC GCAAAGAAGG GAATGAGTGC
3961 GACACGAAAA TGTTGGATGC TCATACTCGT CCTTTTTCAA TATTATTGAA GCATTTATCA
4021 GGGTTACTAG TACGTCTCTC AAGGATAAGT AAGTAATATT AAGGTACGGG AGGTATTGGA
4081 CAGGCCGCAA TAAAATATCT TTATTTTCAT TACATCTGTG TGTTGGTTTT TTGTGTGAAT
4141 CGATAGTACT AACATACGCT CTCCATCAAA ACAAAACGAA ACAAAACAAA CTAGCAAAAT
4201 AGGCTGTCCC CAGTGCAAGT GCAGGTGCCA GAACATTTCT CT
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pGL4.10[luc2]其他相关信号通路报告载体：
