psiCHECK-2

psiCHECK-2

psiCHECK-2载体基本信息：

psiCHECK-2载体质粒图谱和多克隆位点信息：

psiCHECK-2载体简介：

        psiCHECK™-2 Vector is designed to provide a quantitative and rapid approach for optimization of RNA interference (RNAi). The vectors enable the monitoring of changes in expression of a target gene fused to the reporter gene. In both vectors, Renilla luciferase is used as a primary reporter gene, and the gene of interest can be cloned into the multiple cloning region located downstream of the Renilla luciferase translational stop codon. Initiation of the RNAi process toward a gene of interest results in cleavage and subsequent degradation of fusion mRNA. Measurement of decreased Renilla luciferase activity is a convenient indicator of RNAi effect .
       RNAi is a phenomenon by which double-stranded RNA complementary to a target mRNA can specifically inactivate gene function by stimulating the degradation of the target mRNA . Because of the ability to inactivate genes, RNAi has emerged as a powerful tool for analyzing gene function.
       In mammalian systems, including cultured mammalian cells, chemically synthesized double-stranded short interfering RNA molecules (<30 nucleotides; siRNA) result in dsRNA duplexes <30 base pairs in length that induce RNAi .RNAi duplexes >30bp induce the interferon response and nonspecific degradation of mRNA and cannot be used as tools for specific gene silencing .

psiCHECK-2载体序列：

ORIGIN

        1 AGATCTGCGC AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT

       61 TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG

      121 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG

      181 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG

      241 CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC

      301 ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG

      361 CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA

      421 AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGCTG CAGAAGTTGG TCGTGAGGCA

      481 CTGGGCAGGT AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT

      541 TGTCGAGACA GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC

      601 ACTTTGCCTT TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA

      661 GTACTTAATA CGACTCACTA TAGGCTAGCC ACCATGGCTT CCAAGGTGTA CGACCCCGAG

      721 CAACGCAAAC GCATGATCAC TGGGCCTCAG TGGTGGGCTC GCTGCAAGCA AATGAACGTG

      781 CTGGACTCCT TCATCAACTA CTATGATTCC GAGAAGCACG CCGAGAACGC CGTGATTTTT

      841 CTGCATGGTA ACGCTGCCTC CAGCTACCTG TGGAGGCACG TCGTGCCTCA CATCGAGCCC

      901 GTGGCTAGAT GCATCATCCC TGATCTGATC GGAATGGGTA AGTCCGGCAA GAGCGGGAAT

      961 GGCTCATATC GCCTCCTGGA TCACTACAAG TACCTCACCG CTTGGTTCGA GCTGCTGAAC

     1021 CTTCCAAAGA AAATCATCTT TGTGGGCCAC GACTGGGGGG CTTGTCTGGC CTTTCACTAC

     1081 TCCTACGAGC ACCAAGACAA GATCAAGGCC ATCGTCCATG CTGAGAGTGT CGTGGACGTG

     1141 ATCGAGTCCT GGGACGAGTG GCCTGACATC GAGGAGGATA TCGCCCTGAT CAAGAGCGAA

     1201 GAGGGCGAGA AAATGGTGCT TGAGAATAAC TTCTTCGTCG AGACCATGCT CCCAAGCAAG

     1261 ATCATGCGGA AACTGGAGCC TGAGGAGTTC GCTGCCTACC TGGAGCCATT CAAGGAGAAG

     1321 GGCGAGGTTA GACGGCCTAC CCTCTCCTGG CCTCGCGAGA TCCCTCTCGT TAAGGGAGGC

     1381 AAGCCCGACG TCGTCCAGAT TGTCCGCAAC TACAACGCCT ACCTTCGGGC CAGCGACGAT

     1441 CTGCCTAAGA TGTTCATCGA GTCCGACCCT GGGTTCTTTT CCAACGCTAT TGTCGAGGGA

     1501 GCTAAGAAGT TCCCTAACAC CGAGTTCGTG AAGGTGAAGG GCCTCCACTT CAGCCAGGAG

     1561 GACGCTCCAG ATGAAATGGG TAAGTACATC AAGAGCTTCG TGGAGCGCGT GCTGAAGAAC

     1621 GAGCAGTAAT TCTAGGCGAT CGCTCGAGCC CGGGAATTCG TTTAAACCTA GAGCGGCCGC

     1681 TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT TGTGTGAGGA

     1741 TCTAAATGAG TCTTCGGACC TCGCGGGGGC CGCTTAAGCG GTGGTTAGGG TTTGTCTGAC

     1801 GCGGGGGGAG GGGGAAGGAA CGAAACACTC TCATTCGGAG GCGGCTCGGG GTTTGGTCTT

     1861 GGTGGCCACG GGCACGCAGA AGAGCGCCGC GATCCTCTTA AGCACCCCCC CGCCCTCCGT

     1921 GGAGGCGGGG GTTTGGTCGG CGGGTGGTAA CTGGCGGGCC GCTGACTCGG GCGGGTCGCG

     1981 CGCCCCAGAG TGTGACCTTT TCGGTCTGCT CGCAGACCCC CGGGCGGCGC CGCCGCGGCG

     2041 GCGACGGGCT CGCTGGGTCC TAGGCTCCAT GGGGACCGTA TACGTGGACA GGCTCTGGAG

     2101 CATCCGCACG ACTGCGGTGA TATTACCGGA GACCTTCTGC GGGACGAGCC GGGTCACGCG

     2161 GCTGACGCGG AGCGTCCGTT GGGCGACAAA CACCAGGACG GGGCACAGGT ACACTATCTT

     2221 GTCACCCGGA GGCGCGAGGG ACTGCAGGAG CTTCAGGGAG TGGCGCAGCT GCTTCATCCC

     2281 CGTGGCCCGT TGCTCGCGTT TGCTGGCGGT GTCCCCGGAA GAAATATATT TGCATGTCTT

     2341 TAGTTCTATG ATGACACAAA CCCCGCCCAG CGTCTTGTCA TTGGCGAATT CGAACACGCA

     2401 GATGCAGTCG GGGCGGCGCG GTCCCAGGTC CACTTCGCAT ATTAAGGTGA CGCGTGTGGC

     2461 CTCGAACACC GAGCGACCCT GCAGCGACCC GCTTAAAAGC TTGGCATTCC GGTACTGTTG

     2521 GTAAAGCCAC CATGGCCGAT GCTAAGAACA TTAAGAAGGG CCCTGCTCCC TTCTACCCTC

     2581 TGGAGGATGG CACCGCTGGC GAGCAGCTGC ACAAGGCCAT GAAGAGGTAT GCCCTGGTGC

     2641 CTGGCACCAT TGCCTTCACC GATGCCCACA TTGAGGTGGA CATCACCTAT GCCGAGTACT

     2701 TCGAGATGTC TGTGCGCCTG GCCGAGGCCA TGAAGAGGTA CGGCCTGAAC ACCAACCACC

     2761 GCATCGTGGT GTGCTCTGAG AACTCTCTGC AGTTCTTCAT GCCAGTGCTG GGCGCCCTGT

     2821 TCATCGGAGT GGCCGTGGCC CCTGCTAACG ACATTTACAA CGAGCGCGAG CTGCTGAACA

     2881 GCATGGGCAT TTCTCAGCCT ACCGTGGTGT TCGTGTCTAA GAAGGGCCTG CAGAAGATCC

     2941 TGAACGTGCA GAAGAAGCTG CCTATCATCC AGAAGATCAT CATCATGGAC TCTAAGACCG

     3001 ACTACCAGGG CTTCCAGAGC ATGTACACAT TCGTGACATC TCATCTGCCT CCTGGCTTCA

     3061 ACGAGTACGA CTTCGTGCCA GAGTCTTTCG ACAGGGACAA AACCATTGCC CTGATCATGA

     3121 ACAGCTCTGG GTCTACCGGC CTGCCTAAGG GCGTGGCCCT GCCTCATCGC ACCGCCTGTG

     3181 TGCGCTTCTC TCACGCCCGC GACCCTATTT TCGGCAACCA GATCATCCCC GACACCGCTA

     3241 TTCTGAGCGT GGTGCCATTC CACCACGGCT TCGGCATGTT CACCACCCTG GGCTACCTGA

     3301 TTTGCGGCTT TCGGGTGGTG CTGATGTACC GCTTCGAGGA GGAGCTGTTC CTGCGCAGCC

     3361 TGCAAGACTA CAAAATTCAG TCTGCCCTGC TGGTGCCAAC CCTGTTCAGC TTCTTCGCTA

     3421 AGAGCACCCT GATCGACAAG TACGACCTGT CTAACCTGCA CGAGATTGCC TCTGGCGGCG

     3481 CCCCACTGTC TAAGGAGGTG GGCGAAGCCG TGGCCAAGCG CTTTCATCTG CCAGGCATCC

     3541 GCCAGGGCTA CGGCCTGACC GAGACAACCA GCGCCATTCT GATTACCCCA GAGGGCGACG

     3601 ACAAGCCTGG CGCCGTGGGC AAGGTGGTGC CATTCTTCGA GGCCAAGGTG GTGGACCTGG

     3661 ACACCGGCAA GACCCTGGGA GTGAACCAGC GCGGCGAGCT GTGTGTGCGC GGCCCTATGA

     3721 TTATGTCCGG CTACGTGAAT AACCCTGAGG CCACAAACGC CCTGATCGAC AAGGACGGCT

     3781 GGCTGCACTC TGGCGACATT GCCTACTGGG ACGAGGACGA GCACTTCTTC ATCGTGGACC

     3841 GCCTGAAGTC TCTGATCAAG TACAAGGGCT ACCAGGTGGC CCCAGCCGAG CTGGAGTCTA

     3901 TCCTGCTGCA GCACCCTAAC ATTTTCGACG CCGGAGTGGC CGGCCTGCCC GACGACGATG

     3961 CCGGCGAGCT GCCTGCCGCC GTCGTCGTGC TGGAACACGG CAAGACCATG ACCGAGAAGG

     4021 AGATCGTGGA CTATGTGGCC AGCCAGGTGA CAACCGCCAA GAAGCTGCGC GGCGGAGTGG

     4081 TGTTCGTGGA CGAGGTGCCC AAGGGCCTGA CCGGCAAGCT GGACGCCCGC AAGATCCGCG

     4141 AGATCCTGAT CAAGGCTAAG AAAGGCGGCA AGATCGCCGT GTAATAATTC TAGAGTCGGG

     4201 GCGGCCGGCC GCTTCGAGCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA

     4261 CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG

     4321 TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC

     4381 AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA

     4441 AAATCGATAA GGATCCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT

     4501 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC

     4561 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC

     4621 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA

     4681 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG

     4741 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG

     4801 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC

     4861 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA

     4921 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG

     4981 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA

     5041 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC

     5101 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT

     5161 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG

     5221 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA

     5281 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA

     5341 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA

     5401 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG

     5461 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG

     5521 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT

     5581 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG

     5641 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC

     5701 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA

     5761 CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA

     5821 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC

     5881 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG

     5941 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC

     6001 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG

     6061 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT

     6121 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT

     6181 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG

     6241 CCTTTTGCTG GCCTTTTGCT CACATGGCTC GAC

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psiCHECK-2其他相关信号通路报告载体：