pSTAT3-TA-luc
pSTAT3-TA-luc
编号 | 载体名称 |
北京华越洋VECT90290 | pSTAT3-TA-luc |
pSTAT3-TA-luc载体基本信息：
启动子: | SV40 |
复制子: | pUC ori，SV40 ori |
终止子: | SV40 poly(A) signal |
质粒分类: | 哺乳细胞，信号通路报告载体 |
质粒大小: | 5672bp |
原核抗性: | Amp |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧 LB |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | 根据序列设计引物 |
3'测序引物: | 根据序列设计引物 |
pSTAT3-TA-luc载体质粒图谱和多克隆位点信息：
pSTAT3-TA-luc载体简介：
PSTAT3-TA-luc can be transfected into cells by conventional cell transfection method. The biyuntian firefly firefly firefly firefly luciferase reporter gene assay kit detection (RG005/RG006) or dual luciferase reporter gene assay kit (RG027/RG028).
STAT3 reagent can be activated, can be used as a positive control pSTAT3-TA-luc report gene detection.
pSTAT3-TA-luc载体序列：
ORIGIN
1 GGCCTAACTG GCCGGTACCG CTAGCCTCGA GTGCTTCCCG AACGTTGCTT CCCGAACGTT
61 GCTTCCCGAA CGTTGCTTCC GAACGTAGAT CTGCAGAAGC TTAGACACTA GAGGGTATAT
121 AATGGAAGCT CGACTTCCAG CTTGGCAATC CGGTACTGTT GGTAAAGCCA CCATGGAAGA
181 TGCCAAAAAC ATTAAGAAGG GCCCAGCGCC ATTCTACCCA CTCGAAGACG GGACCGCCGG
241 CGAGCAGCTG CACAAAGCCA TGAAGCGCTA CGCCCTGGTG CCCGGCACCA TCGCCTTTAC
301 CGACGCACAT ATCGAGGTGG ACATTACCTA CGCCGAGTAC TTCGAGATGA GCGTTCGGCT
361 GGCAGAAGCT ATGAAGCGCT ATGGGCTGAA TACAAACCAT CGGATCGTGG TGTGCAGCGA
421 GAATAGCTTG CAGTTCTTCA TGCCCGTGTT GGGTGCCCTG TTCATCGGTG TGGCTGTGGC
481 CCCAGCTAAC GACATCTACA ACGAGCGCGA GCTGCTGAAC AGCATGGGCA TCAGCCAGCC
541 CACCGTCGTA TTCGTGAGCA AGAAAGGGCT GCAAAAGATC CTCAACGTGC AAAAGAAGCT
601 ACCGATCATA CAAAAGATCA TCATCATGGA TAGCAAGACC GACTACCAGG GCTTCCAAAG
661 CATGTACACC TTCGTGACTT CCCATTTGCC ACCCGGCTTC AACGAGTACG ACTTCGTGCC
721 CGAGAGCTTC GACCGGGACA AAACCATCGC CCTGATCATG AACAGTAGTG GCAGTACCGG
781 ATTGCCCAAG GGCGTAGCCC TACCGCACCG CACCGCTTGT GTCCGATTCA GTCATGCCCG
841 CGACCCCATC TTCGGCAACC AGATCATCCC CGACACCGCT ATCCTCAGCG TGGTGCCATT
901 TCACCACGGC TTCGGCATGT TCACCACGCT GGGCTACTTG ATCTGCGGCT TTCGGGTCGT
961 GCTCATGTAC CGCTTCGAGG AGGAGCTATT CTTGCGCAGC TTGCAAGACT ATAAGATTCA
1021 ATCTGCCCTG CTGGTGCCCA CACTATTTAG CTTCTTCGCT AAGAGCACTC TCATCGACAA
1081 GTACGACCTA AGCAACTTGC ACGAGATCGC CAGCGGCGGG GCGCCGCTCA GCAAGGAGGT
1141 AGGTGAGGCC GTGGCCAAAC GCTTCCACCT ACCAGGCATC CGCCAGGGCT ACGGCCTGAC
1201 AGAAACAACC AGCGCCATTC TGATCACCCC CGAAGGGGAC GACAAGCCTG GCGCAGTAGG
1261 CAAGGTGGTG CCCTTCTTCG AGGCTAAGGT GGTGGACTTG GACACCGGTA AGACACTGGG
1321 TGTGAACCAG CGCGGCGAGC TGTGCGTCCG TGGCCCCATG ATCATGAGCG GCTACGTTAA
1381 CAACCCCGAG GCTACAAACG CTCTCATCGA CAAGGACGGC TGGCTGCACA GCGGCGACAT
1441 CGCCTACTGG GACGAGGACG AGCACTTCTT CATCGTGGAC CGGCTGAAGA GCCTGATCAA
1501 ATACAAGGGC TACCAGGTAG CCCCAGCCGA ACTGGAGAGC ATCCTGCTGC AACACCCCAA
1561 CATCTTCGAC GCCGGGGTCG CCGGCCTGCC CGACGACGAT GCCGGCGAGC TGCCCGCCGC
1621 AGTCGTCGTG CTGGAACACG GTAAAACCAT GACCGAGAAG GAGATCGTGG ACTATGTGGC
1681 CAGCCAGGTT ACAACCGCCA AGAAGCTGCG CGGTGGTGTT GTGTTCGTGG ACGAGGTGCC
1741 TAAAGGACTG ACCGGCAAGT TGGACGCCCG CAAGATCCGC GAGATTCTCA TTAAGGCCAA
1801 GAAGGGCGGC AAGATCGCCG TGTAATAATT CTAGAGTCGG GGCGGCCGGC CGCTTCGAGC
1861 AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA
1921 ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA
1981 TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG
2041 GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT AAAATCGATA AGGATCCGTT
2101 TGCGTATTGG GCGCTCTTCC GCTGATCTGC GCAGCACCAT GGCCTGAAAT AACCTCTGAA
2161 AGAGGAACTT GGTTAGCTAC CTTCTGAGGC GGAAAGAACC AGCTGTGGAA TGTGTGTCAG
2221 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC
2281 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA
2341 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC
2401 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT
2461 GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT
2521 GGAGGCCTAG GCTTTTGCAA AAAGCTCGAT TCTTCTGACA CTAGCGCCAC CATGATCGAA
2581 CAAGACGGCC TCCATGCTGG CAGTCCCGCA GCTTGGGTCG AACGCTTGTT CGGGTACGAC
2641 TGGGCCCAGC AGACCATCGG ATGTAGCGAT GCGGCCGTGT TCCGTCTAAG CGCTCAAGGC
2701 CGGCCCGTGC TGTTCGTGAA GACCGACCTG AGCGGCGCCC TGAACGAGCT TCAAGACGAG
2761 GCTGCCCGCC TGAGCTGGCT GGCCACCACC GGCGTACCCT GCGCCGCTGT GTTGGATGTT
2821 GTGACCGAAG CCGGCCGGGA CTGGCTGCTG CTGGGCGAGG TCCCTGGCCA GGATCTGCTG
2881 AGCAGCCACC TTGCCCCCGC TGAGAAGGTT TCTATCATGG CCGATGCAAT GCGGCGCCTG
2941 CACACCCTGG ACCCCGCTAC CTGCCCCTTC GACCACCAGG CTAAGCATCG GATCGAGCGT
3001 GCTCGGACCC GCATGGAGGC CGGCCTGGTG GACCAGGACG ACCTGGACGA GGAGCATCAG
3061 GGCCTGGCCC CCGCTGAACT GTTCGCCCGA CTGAAAGCCC GCATGCCGGA CGGTGAGGAC
3121 CTGGTTGTCA CACACGGAGA TGCCTGCCTC CCTAACATCA TGGTCGAGAA TGGCCGCTTC
3181 TCCGGCTTCA TCGACTGCGG TCGCCTAGGA GTTGCCGACC GCTACCAGGA CATCGCCCTG
3241 GCCACCCGCG ACATCGCTGA GGAGCTTGGC GGCGAGTGGG CCGACCGCTT CTTAGTCTTG
3301 TACGGCATCG CAGCTCCCGA CAGCCAGCGC ATCGCCTTCT ACCGCTTGCT CGACGAGTTC
3361 TTTTAATGAT CTAGAACCGG TCATGGCCGC AATAAAATAT CTTTATTTTC ATTACATCTG
3421 TGTGTTGGTT TTTTGTGTGT TCGAACTAGA TGCTGTCGAC CGATGCCCTT GAGAGCCTTC
3481 AACCCAGTCA GCTCCTTCCG GTGGGCGCGG GGCATGACTA TCGTCGCCGC ACTTATGACT
3541 GTCTTCTTTA TCATGCAACT CGTAGGACAG GTGCCGGCAG CGCTCTTCCG CTTCCTCGCT
3601 CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC
3661 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG
3721 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG
3781 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
3841 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
3901 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
3961 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
4021 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
4081 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
4141 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
4201 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
4261 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
4321 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
4381 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA
4441 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT
4501 ATATGAGTAA ACTTGGTCTG ACAGCGGCCG CAAATGCTAA ACCACTGCAG TGGTTACCAG
4561 TGCTTGATCA GTGAGGCACC GATCTCAGCG ATCTGCCTAT TTCGTTCGTC CATAGTGGCC
4621 TGACTCCCCG TCGTGTAGAT CACTACGATT CGTGAGGGCT TACCATCAGG CCCCAGCGCA
4681 GCAATGATGC CGCGAGAGCC GCGTTCACCG GCCCCCGATT TGTCAGCAAT GAACCAGCCA
4741 GCAGGGAGGG CCGAGCGAAG AAGTGGTCCT GCTACTTTGT CCGCCTCCAT CCAGTCTATG
4801 AGCTGCTGTC GTGATGCTAG AGTAAGAAGT TCGCCAGTGA GTAGTTTCCG AAGAGTTGTG
4861 GCCATTGCTA CTGGCATCGT GGTATCACGC TCGTCGTTCG GTATGGCTTC GTTCAACTCT
4921 GGTTCCCAGC GGTCAAGCCG GGTCACATGA TCACCCATAT TATGAAGAAA TGCAGTCAGC
4981 TCCTTAGGGC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CGGTGTTGTC GCTCATGGTA
5041 ATGGCAGCAC TACACAATTC TCTTACCGTC ATGCCATCCG TAAGATGCTT TTCCGTGACC
5101 GGCGAGTACT CAACCAAGTC GTTTTGTGAG TAGTGTATAC GGCGACCAAG CTGCTCTTGC
5161 CCGGCGTCTA TACGGGACAA CACCGCGCCA CATAGCAGTA CTTTGAAAGT GCTCATCATC
5221 GGGAATCGTT CTTCGGGGCG GAAAGACTCA AGGATCTTGC CGCTATTGAG ATCCAGTTCG
5281 ATATAGCCCA CTCTTGCACC CAGTTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCG
5341 GGGTGTGCAA AAACAGGCAA GCAAAATGCC GCAAAGAAGG GAATGAGTGC GACACGAAAA
5401 TGTTGGATGC TCATACTCGT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTACTAG
5461 TACGTCTCTC AAGGATAAGT AAGTAATATT AAGGTACGGG AGGTATTGGA CAGGCCGCAA
5521 TAAAATATCT TTATTTTCAT TACATCTGTG TGTTGGTTTT TTGTGTGAAT CGATAGTACT
5581 AACATACGCT CTCCATCAAA ACAAAACGAA ACAAAACAAA CTAGCAAAAT AGGCTGTCCC
5641 CAGTGCAAGT GCAGGTGCCA GAACATTTCT CT
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pSTAT3-TA-luc其他相关信号通路报告载体：
