首页 ꄲ 载体及质粒 ꄲ pmCherry-EGFP-LC3B-h

pmCherry-EGFP-LC3B-h

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pmCherry-EGFP-LC3B-h

pmCherry-EGFP-LC3B-h

编号	载体名称
北京华越洋VECT90326	pmCherry-EGFP-LC3B-h

pmCherry-EGFP-LC3B-h载体基本信息：

启动子:	CMV
复制子:	pUC ori,f1 ori,SV40 ori
终止子:	SV40 poly(A) signal
质粒分类:	哺乳细胞，自噬表达载体
质粒大小:	5833bp
原核抗性:	Kan
筛选标记:	Neo
克隆菌株:	DH5α
培养条件:	37℃，有氧 LB
表达宿主:	哺乳细胞
诱导方式:	无须诱导，瞬时表达
5'测序引物:	CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物:	Sv40-polyA-R：GAAATTTGTGATGCTATTGC

pmCherry-EGFP-LC3B-h载体质粒图谱和多克隆位点信息：

pmCherry-EGFP-LC3B-h载体简介：

PmCherry-EGFP-LC3b-h é um estudo para células de mamíferos que autophagy, vetor de expressão EM células de mamíferos, com muitas Linhas de células de mamíferos, por exemplo, Pode ser a expressão de UMA proteína Verde fluorescente, e um corpo Vermelho com proteínas e proteínas fluorescentes, LC3b-h juntos.

pmCherry-EGFP-LC3B-h载体序列：

ORIGIN

1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG

61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT

121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA

181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC

241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA

301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC

361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG

421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG

481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT

541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCCGCC

601 ACCATGGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC

661 AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG

721 GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG

781 CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG

841 CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG

901 CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG

961 GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC

1021 GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC

1081 GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC

1141 GCTGAGGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC

1201 GTCAACATCA AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC

1261 GAACGCGCCG AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA GTCCGGACTC

1321 AGATCTCGAG GCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG

1381 GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC

1441 GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG

1501 CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC

1561 GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG

1621 CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG

1681 GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC

1741 ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC

1801 AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC

1861 GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG

1921 CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC

1981 GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG

2041 CTGTACAAGT ACTCAGATCT CGAGCTCAAG CTTCGAATTC CCATGCCGTC GGAGAAGACC

2101 TTCAAGCAGC GCCGCACCTT CGAACAAAGA GTAGAAGATG TCCGACTTAT TCGAGAGCAG

2161 CATCCAACCA AAATCCCGGT GATAATAGAA CGATACAAGG GTGAGAAGCA GCTTCCTGTT

2221 CTGGATAAAA CAAAGTTCCT TGTACCTGAC CATGTCAACA TGAGTGAGCT CATCAAGATA

2281 ATTAGAAGGC GCTTACAGCT CAATGCTAAT CAGGCCTTCT TCCTGTTGGT GAACGGACAC

2341 AGCATGGTCA GCGTCTCCAC ACCAATCTCA GAGGTGTATG AGAGTGAGAA AGATGAAGAT

2401 GGATTCCTGT ACATGGTCTA TGCCTCCCAG GAGACGTTCG GGATGAAATT GTCAGTGTAA

2461 GAATTCTGCA GTCGACGGTA CCGCGGGCCC GGGATCCACC GGATCTAGAT AACTGATCAT

2521 AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC

2581 CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA

2641 TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT

2701 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TAACGCGTAA ATTGTAAGCG

2761 TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT

2821 AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG

2881 TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC

2941 GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA TCAAGTTTTT

3001 TGGGGTCGAG GTGCCGTAAA GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG

3061 CTTGACGGGG AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG

3121 GCGCTAGGGC GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC

3181 TTAATGCGCC GCTACAGGGC GCGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC

3241 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT

3301 GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTCCTGA GGCGGAAAGA ACCAGCTGTG

3361 GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA

3421 AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG

3481 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC

3541 GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT

3601 TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG

3661 AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAGATCGA TCAAGAGACA GGATGAGGAT

3721 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA

3781 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC

3841 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA

3901 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG

3961 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC

4021 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG

4081 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA

4141 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC

4201 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA

4261 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG

4321 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT

4381 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG

4441 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC

4501 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC

4561 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT

4621 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA

4681 GTTCTTCGCC CACCCTAGGG GGAGGCTAAC TGAAACACGG AAGGAGACAA TACCGGAAGG

4741 AACCCGCGCT ATGACGGCAA TAAAAAGACA GAATAAAACG CACGGTGTTG GGTCGTTTGT

4801 TCATAAACGC GGGGTTCGGT CCCAGGGCTG GCACTCTGTC GATACCCCAC CGAGACCCCA

4861 TTGGGGCCAA TACGCCCGCG TTTCTTCCTT TTCCCCACCC CACCCCCCAA GTTCGGGTGA

4921 AGGCCCAGGG CTCGCAGCCA ACGTCGGGGC GGCAGGCCCT GCCATAGCCT CAGGTTACTC

4981 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT

5041 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC

5101 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG

5161 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT

5221 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT

5281 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT

5341 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG

5401 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC

5461 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA

5521 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG

5581 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA

5641 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG

5701 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG

5761 CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT

5821 TACCGCCATG CAT

pmCherry-EGFP-LC3B-h其他相关四环素调控载体：

pEF1/V5-HisA	pEGFP-N3
pEF-GFP	pEGFP-C3
pFLAG-CMV2	pEGFP-N2
pCI-NEO	pEGFP-N1
pCEP4	pEGFP-1
pVAX1	pECFP-C1
pcDNA3.1/V5-His B	pECFP-N1
pCDNA6-Myc-HisB	pDsRed2-Nuc
pCDNA3.1-3×HA	pDsRed2-ER
pCDNA3.1-3×HA	Top-Flash
pCDNA3.1(-)/Myc-His B	pSTAT3-TA-luc
pCDNA3.1(-)/Myc-His A	pSilencer2.1-U6 neo
pCDNA3.1(+)/Myc-His B	psiCHECK-2
pCDNA3.1(+)-Zeo	pRL-TK
pCDNA3.1(+)-EGFP	pRL-SV40
pCDNA3.1(+)	pRL-CMV
pCDNA3.1(-)	pp53-TA-luc
pBudCE4.1-hrGFP	pNF-KB-TA-LUC
pCAGGS-HA	pNF-kB-Luc
pVAX1-FLAG	pMIR-Reporter
pSV2-DHFR	pmirGLO
pCEP4/CAT	pGL4.79[hRluc/Neo]
pCAGGS	pGL4.75[hRluc/CMV]
pCAG-GFP	pGL4.73[hRluc /SV40]
pCDNA3.0	pGL4.29[luc2P/CRE/Hygro]
pCDNA3.0-HA	pGL4.13-luci2
pCDNA3.1(+)-3×FLAG	pGL4.13-luci2
pSV-Beta-Gal	pGL4.10[luc2]
SuperCos1	pGL3-Enhancer
pT2/VENUS	pGL3-Control
pAdVAntage	pGL3-Basic
pEYFP-Tubulin	pGL3-3.6kb human IFN-gamma luc
pEGFP-C1	pENTR5'-UbCp
pmGeos-M-N1	pBiFC-VN173
pEYFP-N1	pBiFC-VC155
pEYFP-C1	pBIFC-bFosVC155
pEGFP-C2	pBiFC-bFOSDelataZipVC155/pBiFC-bFos(deltaZIP) VC155
pEYFP-Actin	Laconic/pcDNA3.1(-)
pDsRed2-Peroxi	Fop-Flash
pEBFP-C1	pTet-On-Advanced
pmGeos-M-C1	pmCherry-EGFP-LC3b-r
pCerulean-N1	pmCherry-EGFP-LC3b-m
pmKO2-N1	pmCherry-EGFP-LC3B-h
pMyosin-PSmOrange	pEGFP-LC3B-r
pTagBFP-N	pEGFP-LC3B-m
pCAG-DsRed	pEGFP-LC3B-h
pmCherry-C1	pEGFP-LC3B-c
pmCherry-N1	Super PiggyBac Transposase (PB200PA-1)
pEYFP-Golgi	pTol2-Promoter-T2A-EGFP
pEYFP-Mem	pSP64-Tol2-Transposase
pDsRed2-C1	PiggyBac Dual promoter (PB513B-1)
pEGFP-Actin	pBIND-ID
pEBFP-N1	pG5Luc
pDsRed2-N1	pFUSE-hIgG1e1-Fc2
pN3	pEE12.4
pEGFP-EcoRV	pEE6.4