pSV2-DHFR

pSV2-DHFR

编号	载体名称
北京华越洋VECT90222	pSV2-DHFR

pSV2-DHFR载体基本信息：

启动子:	SV 40
复制子:	PMB1 ori
终止子:	SV40 poly(A) signal
质粒分类:	哺乳细胞载体;蛋白过表达质粒
质粒大小:	4900bp
质粒标签:	C-DHFR ( mouse DHFR)
原核抗性:	氨苄青霉素Amp
筛选标记:	甲氨蝶呤MTX
克隆菌株:	DH5α
培养条件:	37℃，有氧，LB
表达宿主:	哺乳细胞
5'测序引物:	SV40pro-F: TATTTATGCAGAGGCCGAGG
3'测序引物:	SV40pA-R: GAAATTTGTGATGCTATTGC
备注:	pSV2-DHFR载体含有的是小鼠的DHFR基因，同时该载体是哺乳动物细胞和大肠杆菌穿梭质粒，在DHFR细胞系中，可以通过甲氨蝶呤对转染的细胞进行筛选。

pSV2-DHFR载体质粒图谱和多克隆位点信息:

pSV2-DHFR载体简介

pSV2-DHFR载体序列：

ORIGIN
        1 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT
       61 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA
      121 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA
      181 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA
      241 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG
      301 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTTATCC CCGCTGCCAT
      361 CATGGTTCGA CCATTGAACT GCATCGTCGC CGTGTCCCAA GATATGGGGA TTGGCAAGAA
      421 CGGAGACCTA CCCTGGCCTC CGCTCAGGAA CGAGTTCAAG TACTTCCAAA GAATGACCAC
      481 AACCTCTTCA GTGGAAGGTA AACAGAATCT GGTGATTATG GGTAGGAAAA CCTGGTTCTC
      541 CATTCCTGAG AAGAATCGAC CTTTAAAGGA CAGAATTAAT ATAGTTCTCA GTAGAGAACT
      601 CAAAGAACCA CCACGAGGAG CTCATTTTCT TGCCAAAAGT TTGGATGATG CCTTAAGACT
      661 TATTGAACAA CCGGAATTGG CAAGTAAAGT AGACATGGTT TGGATAGTCG GAGGCAGTTC
      721 TGTTTACCAG GAAGCCATGA ATCAACCAGG CCACCTCAGA CTCTTTGTGA CAAGGATCAT
      781 GCAGGAATTT GAAAGTGACA CGTTTTTCCC AGAAATTGAT TTGGGGAAAT ATAAACTTCT
      841 CCCAGAATAC CCAGGCGTCC TCTCTGAGGT CCAGGAGGAA AAAGGCATCA AGTATAAGTT
      901 TGAAGTCTAC GAGAAGAAAG ACTAACAGGA AGATGCTTTC AAGTTCTCTG CTCCCCTCCT
      961 AAAGCTATGC ATTTTTATAA GACCATGGGA CTTTTGCTGG CTTTAGATCT TTGTGAAGGA
     1021 ACCTTACTTC TGTGGTGTGA CATAATTGGA CAAACTACCT ACAGAGATTT AAAGCTCTAA
     1081 GGTAAATATA AAATTTTTAA GTGTATAATG TGTTAAACTA CTGATTCTAA TTGTTTGTGT
     1141 ATTTTAGATT CCAACCTATG GAACTGATGA ATGGGAGCAG TGGTGGAATG CCTTTAATGA
     1201 GGAAAACCTG TTTTGCTCAG AAGAAATGCC ATCTAGTGAT GATGAGGCTA CTGCTGACTC
     1261 TCAACATTCT ACTCCTCCAA AAAAGAAGAG AAAGGTAGAA GACCCCAAGG ACTTTCCTTC
     1321 AGAATTGCTA AGTTTTTTGA GTCATGCTGT GTTTAGTAAT AGAACTCTTG CTTGCTTTGC
     1381 TATTTACACC ACAAAGGAAA AAGCTGCACT GCTATACAAG AAAATTATGG AAAAATATTC
     1441 TGTAACCTTT ATAAGTAGGC ATAACAGTTA TAATCATAAC ATACTGTTTT TTCTTACTCC
     1501 ACACAGGCAT AGAGTGTCTG CTATTAATAA CTATGCTCAA AAATTGTGTA CCTTTAGCTT
     1561 TTTAATTTGT AAAGGGGTTA ATAAGGAATA TTTGATGTAT AGTGCCTTGA CTAGAGATCA
     1621 TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC
     1681 CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT
     1741 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC
     1801 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCCCCA
     1861 GGAAGCTCCT CTGTGTCCTC ATAAACCCTA ACCTCCTCTA CTTGAGAGGA CATTCCAATC
     1921 ATAGGCTGCC CATCCACCCT CTGTGTCCTC CTGTTAATTA GGTCACTTAA CAAAAAGGAA
     1981 ATTGGGTAGG GGTTTTTCAC AGACCGCTTT CTAAGGGTAA TTTTAAAATA TCTGGGAAGT
     2041 CCCTTCCACT GCTGTGTTCC AGAAGTGTTG GTAAACAGCC CACAAATGTC AACAGCAGAA
     2101 ACATACAAGC TGTCAGCTTT GCACAAGGGC CCAACACCCT GCTCATCAAG AAGCACTGTG
     2161 GTTGCTGTGT TAGTAATGTG CAAAACAGGA GGCACATTTT CCCCACCTGT GTAGGTTCCA
     2221 AAATATCTAG TGTTTTCATT TTTACTTGGA TCAGGAACCC AGCACTCCAC TGGATAAGCA
     2281 TTATCCTTAT CCAAAACAGC CTTGTGGTCA GTGTTCATCT GCTGACTGTC AACTGTAGCA
     2341 TTTTTTGGGG TTACAGTTTG AGCAGGATAT TTGGTCCTGT AGTTTGCTAA CACACCCTGC
     2401 AGCTCCAAAG GTTCCCCACC AACAGCAAAA AAATGAAAAT TTGACCCTTG AATGGGTTTT
     2461 CCAGCACCAT TTTCATGAGT TTTTTGTGTC CCTGAATGCA AGTTTAACAT AGCAGTTACC
     2521 CCAATAACCT CAGTTTTAAC AGTAACAGCT TCCCACATCA AAATATTTCC ACAGGTTAAG
     2581 TCCTCATTTA AATTAGGCAA AGGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT
     2641 TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG
     2701 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC
     2761 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA
     2821 TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG
     2881 CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG
     2941 GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC
     3001 GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG
     3061 ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT
     3121 ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG
     3181 CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC
     3241 CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT
     3301 GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG
     3361 CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC
     3421 AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC
     3481 TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA
     3541 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG
     3601 GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA
     3661 TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC
     3721 TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA
     3781 TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT
     3841 CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC
     3901 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG
     3961 GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC
     4021 ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG
     4081 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG
     4141 ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA
     4201 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG
     4261 AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA
     4321 GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT
     4381 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA
     4441 GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC
     4501 CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG
     4561 CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC
     4621 TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC
     4681 TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG
     4741 TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC
     4801 TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG
     4861 TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG
//

pSV2-DHFR其他相关哺乳细胞载体：

pEF1/V5-HisA	pEGFP-N3
pEF-GFP	pEGFP-C3
pFLAG-CMV2	pEGFP-N2
pCI-NEO	pEGFP-N1
pCEP4	pEGFP-1
pVAX1	pECFP-C1
pcDNA3.1/V5-His B	pECFP-N1
pCDNA6-Myc-HisB	pDsRed2-Nuc
pCDNA3.1-3×HA	pDsRed2-ER
pCDNA3.1-3×HA	Top-Flash
pCDNA3.1(-)/Myc-His B	pSTAT3-TA-luc
pCDNA3.1(-)/Myc-His A	pSilencer2.1-U6 neo
pCDNA3.1(+)/Myc-His B	psiCHECK-2
pCDNA3.1(+)-Zeo	pRL-TK
pCDNA3.1(+)-EGFP	pRL-SV40
pCDNA3.1(+)	pRL-CMV
pCDNA3.1(-)	pp53-TA-luc
pBudCE4.1-hrGFP	pNF-KB-TA-LUC
pCAGGS-HA	pNF-kB-Luc
pVAX1-FLAG	pMIR-Reporter
pSV2-DHFR	pmirGLO
pCEP4/CAT	pGL4.79[hRluc/Neo]
pCAGGS	pGL4.75[hRluc/CMV]
pCAG-GFP	pGL4.73[hRluc /SV40]
pCDNA3.0	pGL4.29[luc2P/CRE/Hygro]
pCDNA3.0-HA	pGL4.13-luci2
pCDNA3.1(+)-3×FLAG	pGL4.13-luci2
pSV-Beta-Gal	pGL4.10[luc2]
SuperCos1	pGL3-Enhancer
pT2/VENUS	pGL3-Control
pAdVAntage	pGL3-Basic
pEYFP-Tubulin	pGL3-3.6kb human IFN-gamma luc
pEGFP-C1	pENTR5'-UbCp
pmGeos-M-N1	pBiFC-VN173
pEYFP-N1	pBiFC-VC155
pEYFP-C1	pBIFC-bFosVC155
pEGFP-C2	pBiFC-bFOSDelataZipVC155/pBiFC-bFos(deltaZIP) VC155
pEYFP-Actin	Laconic/pcDNA3.1(-)
pDsRed2-Peroxi	Fop-Flash
pEBFP-C1	pTet-On-Advanced
pmGeos-M-C1	pmCherry-EGFP-LC3b-r
pCerulean-N1	pmCherry-EGFP-LC3b-m
pmKO2-N1	pmCherry-EGFP-LC3B-h
pMyosin-PSmOrange	pEGFP-LC3B-r
pTagBFP-N	pEGFP-LC3B-m
pCAG-DsRed	pEGFP-LC3B-h
pmCherry-C1	pEGFP-LC3B-c
pmCherry-N1	Super PiggyBac Transposase (PB200PA-1)
pEYFP-Golgi	pTol2-Promoter-T2A-EGFP
pEYFP-Mem	pSP64-Tol2-Transposase
pDsRed2-C1	PiggyBac Dual promoter (PB513B-1)
pEGFP-Actin	pBIND-ID
pEBFP-N1	pG5Luc
pDsRed2-N1	pFUSE-hIgG1e1-Fc2
pN3	pEE12.4
pEGFP-EcoRV	pEE6.4