pMMB207
pMMB207
编号 | 载体名称 |
北京华越洋VECT75125 | pMMB207 |
pMMB207载体基本信息：
载体名称: | pMMB207 |
质粒类型: | 细菌表达载体；广宿主载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶，多克隆位点 |
启动子: | Tac |
载体大小: | 9075 bp |
5' 测序引物及序列 | Tac promoter：GAGCGGATAACAATTTCACACAGG |
3' 测序引物及序列: | pBAD Reverse: GATTTAATCTGTATCAGG |
载体标签: | -- |
载体抗性: | 氨苄青霉素和氯霉素（10 mg/ml Chloramphenicol） |
表达菌株: | 原核细胞 |
备注: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pMMB207载体质粒图谱和多克隆位点信息：
pMMB207载体序列：
ORIGIN
1 ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc
61 ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc
121 gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa
181 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
241 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg
301 cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag
361 gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat
421 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
481 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
541 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
601 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
661 gagttttcgc cccgaagaac gtgatgtccg gcggtgcttt tgccgttacg caccaccccg
721 tcagtagctg aacaggaggg acagctgata gaaacagaag ccactggagc aagcacctca
781 aaaacaccat catacactaa atcagtaagt tggcagcatc acccgacgca ctttgcgccg
841 aataaatacc tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg
901 ataccgggaa gccctgggcc aacttttggc gaaaatgaga cgttgatcgg cacgtaagag
961 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga
1021 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
1081 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
1141 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
1201 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
1261 aattacgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
1321 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
1381 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
1441 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
1501 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
1561 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
1621 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
1681 gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattgtctt caaattcccg
1741 ttgcacatag cccggcaatt cctttccctg ctctgccata agcgcagcga atgccgggta
1801 atactcgtca acgatctgat agagaagggt ttgctcgggt cggtggctct ggtaacgacc
1861 agtatcccga tcccggctgg ccgtcctggc cgccacatga ggcatgttcc gcgtccttgc
1921 aatactgtgt ttacatacag tctatcgctt agcggaaagt tcttttaccc tcagccgaaa
1981 tgcctgccgt tgctagacat tgccagccag tgcccgtcac tcccgtacta actgtcacga
2041 acccctgcaa taactgtcac gcccccctgc aataactgtc acgaacccct gcaataactg
2101 tcacgccccc aaacctgcaa acccagcagg ggcgggggct ggcggggtgt tggaaaaatc
2161 catccatgat tatctaagaa taatccacta ggcgcggtta tcagcgccct tgtggggcgc
2221 tgctgccctt gcccaatatg cccggccaga ggccggatag ctggtctatt cgctgcgcta
2281 ggctacacac cgccccaccg ctgcgcggca gggggaaagg cgggcaaagc ccgctaaacc
2341 ccacaccaaa ccccgcagaa atacgctgga gcgcttttag ccgctttagc ggcctttccc
2401 cctacccgaa gggtgggggc gcgtgtgcag ccccgcaggg cctgtctcgg tcgatcattc
2461 agcccggctc atccttctgg cgtggcggca gaccgaacaa ggcgcggtcg tggtcgcgtt
2521 caaggtacgc atccattgcc gccatgagcc gatcctccgg ccactcgctg ctgttcacct
2581 tggccaaaat catggccccc accagcacct tgcgccttgt ttcgttcttg cgctcttgct
2641 gctgttccct tgcccgctcc cgctgaattt cggcattgat tcgcgctcgt tgttcttcga
2701 gcttggccag ccgatccgcc gccttgttgc tccccttaac catcttgaca ccccattgtt
2761 aatgtgctgt ctcgtaggct atcatggagg cacagcggcg gcaatcccga ccctactttg
2821 taggggaggg cgcacttacc ggtttctctt cgagaaactg gcctaacggc cacccttcgg
2881 gcggtgcgct ctccgagggc cattgcatgg agccgaaaag caaaagcaac agcgaggcag
2941 catggcgatt tatcacctta cggcgaaaac cggcagcagg tcgggcggcc aatcggccag
3001 ggccaaggcc gactacatcc agcgcgaagg caagtatgcc cgcgacatgg atgaagtctt
3061 gcacgccgaa tccgggcaca tgccggagtt cgtcgagcgg cccgccgact actgggatgc
3121 tgccgacctg tatgaacgcg ccaatgggcg gctgttcaag gaggtcgaat ttgccctgcc
3181 ggtcgagctg accctcgacc agcagaaggc gctggcgtcc gagttcgccc agcacctgac
3241 cggtgccgag cgcctgccgt atacgctggc catccatgcc ggtggcggcg agaacccgca
3301 ctgccacctg atgatctccg agcggatcaa tgacggcatc gagcggcccg ccgctcagtg
3361 gttcaagcgg tacaacggca agaccccgga gaagggcggg gcacagaaga ccgaagcgct
3421 caagcccaag gcatggcttg agcagacccg cgaggcatgg gccgaccatg ccaaccgggc
3481 attagagcgg gctggccacg acgcccgcat tgaccacaga acacttgagg cgcagggcat
3541 cgagcgcctg cccggtgttc acctggggcc gaacgtggtg gagatggaag gccggggcat
3601 ccgcaccgac cgggcagacg tggccctgaa catcgacacc gccaacgccc agatcatcga
3661 cttacaggaa taccgggagg caatagacca tgaacgcaat cgacagagtg aagaaatcca
3721 gaggcatcaa cgagttagcg gagcagatcg aaccgctggc ccagagcatg gcgacactgg
3781 ccgacgaagc ccggcaggtc atgagccaga ccaagcaggc cagcgaggcg caggcggcgg
3841 agtggctgaa agcccagcgc cagacagggg cggcatgggt ggagctggcc aaagagttgc
3901 gggaggtagc cgccgaggtg agcagcgccg cgcagagcgc ccggagcgcg tcgcgggggt
3961 ggcactggaa gctatggcta accgtgatgc tggcttccat gatgcctacg gtggtgctgc
4021 tgatcgcatc gttgctcttg ctcgacctga cgccactgac aaccgaggac ggctcgatct
4081 ggctgcgctt ggtggcccga tgaagaacga caggactttg caggccatag gccgacagct
4141 caaggccatg ggctgtgagc gcttcgatat cggcgtcagg gacgcaccca ccggccagat
4201 gatgaaccgg gaatggtcag ccgccgaagt gctccagaac acgccatggc tcaagcggat
4261 gaatgcccag ggcaatgacg tgtatatcag gcccgccgag caggagcggc atggtctggt
4321 gctggtggac gacctcagcg agtttgacct ggatgacatg aaagccgagg gccgggagcc
4381 tgccctggta gtggaaacca gcccgaagaa ctatcaggca tgggtcaagg tggccgacgc
4441 cgcaggcggt gaacttcggg ggcagattgc ccggacgctg gccagcgagt acgacgccga
4501 cccggccagc gccgacagcc gccactatgg ccgcttggcg ggcttcacca accgcaagga
4561 caagcacacc acccgcgccg gttatcagcc gtgggtgctg ctgcgtgaat ccaagggcaa
4621 gaccgccacc gctggcccgg cgctggtgca gcaggctggc cagcagatcg agcaggccca
4681 gcggcagcag gagaaggccc gcaggctggc cagcctcgaa ctgcccgagc ggcagcttag
4741 ccgccaccgg cgcacggcgc tggacgagta ccgcagcgag atggccgggc tggtcaagcg
4801 cttcggtcat gacctcagca agtgcgactt tatcgccgcg cagaagctgg ccagccgggg
4861 ccgcagtgcc gaggaaatcg gcaaggccat ggccgaggcc agcccagcgc tggcagagcg
4921 caagcccggc cacgaagcgg attacatcga gcgcaccgtc agcaaggtca tgggtctgcc
4981 cagcgtccag cttgcgcggg ccgagctggc acgggcaccg gcaccccgcc agcgaggcat
5041 ggacaggggc gggccagatt tcagcatgta gtgcttgcgt tggtactcac gcctgttata
5101 ctatgagtac tcacgcacag aagggggttt tatggaatac gaaaaaagcg cttcagggtc
5161 ggtctacctg atcaaaagtg acaagggcta ttggttgccc ggtggctttg gttatacgtc
5221 aaacaaggcc gaggctggcc gcttttcagt cgctgatatg gccagcctta accttgacgg
5281 ctgcaccttg tccttgttcc gcgaagacaa gcctttcggc cccggcaagt ttctcggtga
5341 ctgatatgaa agaccaaaag gacaagcaga ccggcgacct gctggccagc cctgacgctg
5401 tacgccaagc gcgatatgcc gagcgcatga aggccaaagg gatgcgtcag cgcaagttct
5461 ggctgaccga cgacgaatac gaggcgctgc gcgagtgcct ggaagaactc agagcggcgc
5521 agggcggggg tagtgacccc gccagcgcct aaccaccaac tgcctgcaaa ggaggcaatc
5581 aatggctacc cataagccta tcaatattct ggaggcgttc gcagcagcgc cgccaccgct
5641 ggactacgtt ttgcccaaca tggtggccgg tacggtcggg gcgctggtgt cgcccggtgg
5701 tgccggtaaa tccatgctgg ccctgcaact ggccgcacag attgcaggcg ggccggatct
5761 gctggaggtg ggcgaactgc ccaccggccc ggtgatctac ctgcccgccg aagacccgcc
5821 caccgccatt catcaccgcc tgcacgccct tggggcgcac ctcagcgccg aggaacggca
5881 agccgtggct gacggcctgc tgatccagcc gctgatcggc agcctgccca acatcatggc
5941 cccggagtgg ttcgacggcc tcaagcgcgc cgccgagggc cgccgcctga tggtgctgga
6001 cacgctgcgc cggttccaca tcgaggaaga aaacgccagc ggccccatgg cccaggtcat
6061 cggtcgcatg gaggccatcg ccgccgatac cgggtgctct atcgtgttcc tgcaccatgc
6121 cagcaagggc gcggccatga tgggcgcagg cgaccagcag caggccagcc ggggcagctc
6181 ggtactggtc gataacatcc gctggcagtc ctacctgtcg agcatgacca gcgccgaggc
6241 cgaggaatgg ggtgtggacg acgaccagcg ccggttcttc gtccgcttcg gtgtgagcaa
6301 ggccaactat ggcgcaccgt tcgctgatcg gtggttcagg cggcatgacg gcggggtgct
6361 caagcccgcc gtgctggaga ggcagcgcaa gagcaagggg gtgccccgtg gtgaagccta
6421 agaacaagca cagcctcagc cacgtccggc acgacccggc gcactgtctg gcccccggcc
6481 tgttccgtgc cctcaagcgg ggcgagcgca agcgcagcaa gctggacgtg acgtatgact
6541 acggcgacgg caagcggatc gagttcagcg gcccggagcc gctgggcgct gatgatctgc
6601 gcatcctgca agggctggtg gccatggctg ggcctaatgg cctagtgctt ggcccggaac
6661 ccaagaccga aggcggacgg cagctccggc tgttcctgga acccaagtgg gaggccgtca
6721 ccgctgaatg ccatgtggtc aaaggtagct atcgggcgct ggcaaaggaa atcggggcag
6781 aggtcgatag tggtggggcg ctcaagcaca tacaggactg catcgagcgc ctttggaagg
6841 tatccatcat cgcccagaat ggccgcaagc ggcaggggtt tcggctgctg tcggagtacg
6901 ccagcgacga ggcggacggg cgcctgtacg tggccctgaa ccccttgatc gcgcaggccg
6961 tcatgggtgg cggccagcat gtgcgcatca gcatggacga ggtgcgggcg ctggacagcg
7021 aaaccgcccg cctgctgcac cagcggctgt gtggctggat cgaccccggc aaaaccggca
7081 aggcttccat agataccttg tgcggctatg tctggccgtc agaggccagt ggttcgacca
7141 tgcgcaagcg ccgcaagcgg gtgcgcgagg cgttgccgga gctggtcgcg ctgggctgga
7201 cggtaaccga gttcgcggcg ggcaagtacg acatcacccg gcccaaggcg gcaggctgac
7261 cccccccact ctattgtaaa caagacattt ttatctttta tattcaatgg cttattttcc
7321 tgctaattgg taataccatg aaaaatacca tgctcagaaa aggcttaaca atattttgaa
7381 aaattgccta ctgagcgctg ccgcacagct ccataggccg ctttcctggc tttgcttcca
7441 gatgtatgct cttctgctcc cgaacgccag caagacgtag cccagcgcgt cggccagctt
7501 gcaattcgcg ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
7561 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
7621 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
7681 accgcctggc cctgagagag ttgcagcaag cggtccacgt ggtttgcccc agcaggcgaa
7741 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
7801 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
7861 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
7921 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
7981 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
8041 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
8101 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
8161 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
8221 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
8281 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
8341 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
8401 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
8461 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
8521 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
8581 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
8641 cttccgggcg ctatcatgcc ataccgcgaa aggttttgca ccattcgatg gtgtcaacgt
8701 aaatgccgct tcgccttcgc gcgcgaattg caagctgatc cgggcttatc gactgcacgg
8761 tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct gtgcaggtcg
8821 taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata atgttttttg
8881 cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca attaatcatc
8941 ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagaattcg
9001 agctcggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggctgttttg
9061 gcggatgaga gaaga
//
pMMB207其他相关广宿主载体：
