北京华越洋生物
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pBTB-1Map

pBTB-1

@pBTB-1

 

pBTB-1



 

编号

载体名称

北京华越洋VECT75148

pBTB-1

 

pBTB-1载体基本信息:

载体名称:

pBTB-1

质粒类型:

广宿主载体;克隆载体;原核表达载体

高拷贝/低拷贝:

--

克隆方法:

限制性内切酶,多克隆位点

启动子:

araBAD

载体大小:

3784 bp

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

--

载体抗性:

Ampicillin

克隆菌株:

DH5α

宿主细胞(系):

多个菌属

备注:

--

组成型/诱导型:

诱导型

 

pBTB-1载体质粒图谱和多克隆位点信息:

 

 

pBTB-1载体序列:

ORIGIN      

        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga

       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg

      121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca

      181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc

      241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag

      301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc

      361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc

      421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg

      481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt

      541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg

      601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg

      661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac

      721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg

      781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa

      841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat

      901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg

      961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca

     1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg

     1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt

     1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg

     1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca

     1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta

     1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat

     1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg

     1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga

     1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca

     1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa

     1621 ggaagctaaa atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt

     1681 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca

     1741 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag

     1801 tttacgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc

     1861 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca

     1921 gaatgacttg gttgagtact caccagtcac agaaaagcat ctcacggatg gcatgacagt

     1981 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct

     2041 ggcaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt

     2101 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga

     2161 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact

     2221 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggatc

     2281 acttctgcgc tcggccctcc cggctggctg gtttattgct gataaatctg gagccggtga

     2341 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgcatcgt

     2401 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga

     2461 gataggtgcc tcactgatta agcattggta atgaggatcc ccctcaagtc aaaagcctcc

     2521 ggtcggaggc ttttgacttt ctgctatgga ggtcaggtat gatttttatg acaacttgac

     2581 ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt

     2641 gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac

     2701 ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc

     2761 ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga

     2821 cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg

     2881 atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc

     2941 gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc

     3001 cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg

     3061 cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt

     3121 aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg

     3181 agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc

     3241 cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag

     3301 attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt

     3361 ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag

     3421 gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa

     3481 ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac

     3541 caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac

     3601 aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca

     3661 cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg

     3721 acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagagaatt

     3781 cgtc

//

 

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