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首页    载体及质粒    pBTB-4

pBTB-4

pBTB-4

pBTB-4



 

编号

载体名称

北京华越洋VECT75107

pBTB-4

 

pBTB-4载体基本信息:

载体名称:

pBTB-4

质粒类型:

广宿主载体;克隆载体;原核表达载体

高拷贝/低拷贝:

--

克隆方法:

限制性内切酶,多克隆位点

启动子:

araBAD

载体大小:

2936 bp

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

--

载体抗性:

四环素

克隆菌株:

DH5α

宿主细胞(系):

多个菌属

备注:

--

组成型/诱导型:

诱导型

 

pBTB-4载体质粒图谱和多克隆位点信息:

 

pBTB-4载体序列:

ORIGIN      

        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga

       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg

      121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca

      181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc

      241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag

      301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc

      361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc

      421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg

      481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt

      541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg

      601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg

      661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac

      721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg

      781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa

      841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat

      901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg

      961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca

     1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg

     1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt

     1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg

     1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca

     1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta

     1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat

     1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg

     1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga

     1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca

     1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa

     1621 ggaagctaaa atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga

     1681 tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca

     1741 ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt

     1801 tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct

     1861 cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg

     1921 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg

     1981 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag

     2041 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat

     2101 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg

     2161 ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt

     2221 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac

     2281 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg

     2341 cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat

     2401 cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa

     2461 gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc

     2521 gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat

     2581 gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca

     2641 aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc

     2701 gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct

     2761 ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg

     2821 aatggaaggg atccccctca agtcaaaagc ctccggtcgg aggcttttga ctttctgcta

     2881 tggaggtcag gtatgattta aatggtcagt attgagcgat atctagagaa ttcgtc

//

 

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