北京华越洋生物
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首页    载体及质粒    pBT-2
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pBT-2

@pBT-2

 

pBT-2

 



编号

载体名称

北京华越洋VECT75149

pBT-2

 

pBT-2载体基本信息:

载体名称:

pBT-2

质粒类型:

广宿主载体;克隆载体;原核载体

高拷贝/低拷贝:

高拷贝

克隆方法:

限制性内切酶,多克隆位点

启动子:

载体大小:

2558 bp

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

--

载体抗性:

卡那霉素

克隆菌株:

DH5α

宿主细胞(系):

多个菌属

备注:

--

组成型/诱导型:

组成型

 

pBT-2载体质粒图谱和多克隆位点信息:

 

pBT-2载体序列:

ORIGIN      

        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga

       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg

      121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca

      181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc

      241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag

      301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc

      361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc

      421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg

      481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt

      541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg

      601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg

      661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac

      721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg

      781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa

      841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat

      901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg

      961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca

     1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg

     1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt

     1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg

     1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca

     1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta

     1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat

     1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg

     1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga

     1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca

     1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa

     1621 ggaagctaaa atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc

     1681 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg

     1741 tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg

     1801 caaaggtagc gttgccaatg atgttacaga tgagatggtc aggctaaact ggctgacgga

     1861 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact

     1921 caccactgcg atcccaggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg

     1981 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg

     2041 taattgtcct tttaacggcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa

     2101 taacggtttg gttggtgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca

     2161 agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg tcactcatgg

     2221 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt

     2281 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg

     2341 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga

     2401 tatgaataaa ttgcagtttc acttgatgct cgatgagttt ttctgagggc ggatccccct

     2461 caagtcaaaa gcctccggtc ggaggctttt gactttctgc tatggaggtc aggtatgatt

     2521 taaatggtca gtattgagcg atatctagag aattcgtc

//

 

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