pCSDest
pCSDest
编号 | 载体名称 |
北京华越洋VECT75128 | pCSDest |
pCSDest载体基本信息：
载体名称: | pCSDest |
质粒类型: | 克隆载体；哺乳动物表达载体；广宿主载体；体外表达载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | Gateway；限制性内切酶，多克隆位点 |
启动子: | simian CMV IE94、lac、T3、SP6 |
载体大小: | 5808 bp |
5' 测序引物及序列: | SP6 fwd、T3 fwd |
3' 测序引物及序列: | SV40 polyA rev |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
克隆菌株: | DH10B |
表达细胞: | 原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。 |
备注: | pCSDest载体能够在多种细胞中进行高水平的瞬表达。pCSDest载体同时也能进行体外转录和翻译。 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pCSDest载体质粒图谱和多克隆位点信息：
pCSDest载体序列：
ORIGIN
1 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG
61 CTACTTGTTC TTTTTGCAGG ATCCCATCGA TTCGAATTCA AGGATCAACA AGTTTGTACA
121 AAAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA TATATTAAAT TAGATTTTGC
181 ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT CCAGTCATAT TGGCGGCCGC
241 ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATAATGTGT GGATTTTGAG
301 TTAGGATCCG TCGAGATTTT CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA AAATCACTGG
361 ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA CATTTTGAGG CATTTCAGTC
421 AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT ATTACGGCCT TTTTAAAGAC
481 CGTAAAGAAA AATAAGCACA AGTTTTATCC GGCCTTTATT CACATTCTTG CCCGCCTGAT
541 GAATGCTCAT CCGGAATTCC GTATGGCAAT GAAAGACGGT GAGCTGGTGA TATGGGATAG
601 TGTTCACCCT TGTTACACCG TTTTCCATGA GCAAACTGAA ACGTTTTCAT CGCTCTGGAG
661 TGAATACCAC GACGATTTCC GGCAGTTTCT ACACATATAT TCGCAAGATG TGGCGTGTTA
721 CGGTGAAAAC CTGGCCTATT TCCCTAAAGG GTTTATTGAG AATATGTTTT TCGTCTCAGC
781 CAATCCCTGG GTGAGTTTCA CCAGTTTTGA TTTAAACGTG GCCAATATGG ACAACTTCTT
841 CGCCCCCGTT TTCACCATGG GCAAATATTA TACGCAAGGC GACAAGGTGC TGATGCCGCT
901 GGCGATTCAG GTTCATCATG CCGTTTGTGA TGGCTTCCAT GTCGGCAGAA TGCTTAATGA
961 ATTACAACAG TACTGCGATG AGTGGCAGGG CGGGGCGTAA AGATCTGGAT CCGGCTTACT
1021 AAAAGCCAGA TAACAGTATG CGTATTTGCG CGCTGATTTT TGCGGTATAA GAATATATAC
1081 TGATATGTAT ACCCGAAGTA TGTCAAAAAG AGGTATGCTA TGAAGCAGCG TATTACAGTG
1141 ACAGTTGACA GCGACAGCTA TCAGTTGCTC AAGGCATATA TGATGTCAAT ATCTCCGGTC
1201 TGGTAAGCAC AACCATGCAG AATGAAGCCC GTCGTCTGCG TGCCGAACGC TGGAAAGCGG
1261 AAAATCAGGA AGGGATGGCT GAGGTCGCCC GGTTTATTGA AATGAACGGC TCTTTTGCTG
1321 ACGAGAACAG GGGCTGGTGA AATGCAGTTT AAGGTTTACA CCTATAAAAG AGAGAGCCGT
1381 TATCGTCTGT TTGTGGATGT ACAGAGTGAT ATTATTGACA CGCCCGGGCG ACGGATGGTG
1441 ATCCCCCTGG CCAGTGCACG TCTGCTGTCA GATAAAGTCT CCCGTGAACT TTACCCGGTG
1501 GTGCATATCG GGGATGAAAG CTGGCGCATG ATGACCACCG ATATGGCCAG TGTGCCGGTC
1561 TCCGTTATCG GGGAAGAAGT GGCTGATCTC AGCCACCGCG AAAATGACAT CAAAAACGCC
1621 ATTAACCTGA TGTTCTGGGG AATATAAATG TCAGGCTCCC TTATACACAG CCAGTCTGCA
1681 GGTCGACCAT AGTGACTGGA TATGTTGTGT TTTACAGTAT TATGTAGTCT GTTTTTTATG
1741 CAAAATCTAA TTTAATATAT TGATATTTAT ATCATTTTAC GTTTCTCGTT CAGCTTTCTT
1801 GTACAAAGTG GTTGATCCTC TCGAGCCTCT AGAACTATAG TGAGTCGTAT TACGTAGATC
1861 CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA
1921 AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA
1981 ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT
2041 GGGAGGTTTT TTAATTCGCG GCCGCGGCGC CAATGCATTG GGCCCGGTAC CCAGCTTTTG
2101 TTCCCTTTAG TGAGGGTTAA TTGCGCGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT
2161 GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA
2221 AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC
2281 TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG
2341 AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT
2401 CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA
2461 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG
2521 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
2581 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
2641 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
2701 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
2761 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
2821 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
2881 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
2941 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT
3001 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
3061 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
3121 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA
3181 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT
3241 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA
3301 CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC
3361 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG
3421 CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT
3481 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT
3541 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG
3601 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC
3661 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA
3721 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC
3781 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT
3841 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG
3901 TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT
3961 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG
4021 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC
4081 CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC
4141 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA
4201 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG
4261 GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTAAATTG TAAGCGTTAA TATTTTGTTA
4321 AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC
4381 AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG
4441 AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT
4501 CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC
4561 CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG
4621 CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG
4681 GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA
4741 CAGGGCGCGT CCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG
4801 GCCTCTTCGC TATTACGCCA GTCGACCATA GCCAATTCAA TATGGCGTAT ATGGACTCAT
4861 GCCAATTCAA TATGGTGGAT CTGGACCTGT GCCAATTCAA TATGGCGTAT ATGGACTCGT
4921 GCCAATTCAA TATGGTGGAT CTGGACCCCA GCCAATTCAA TATGGCGGAC TTGGCACCAT
4981 GCCAATTCAA TATGGCGGAC TTGGCACTGT GCCAACTGGG GAGGGGTCTA CTTGGCACGG
5041 TGCCAAGTTT GAGGAGGGGT CTTGGCCCTG TGCCAAGTCC GCCATATTGA ATTGGCATGG
5101 TGCCAATAAT GGCGGCCATA TTGGCTATAT GCCAGGATCA ATATATAGGC AATATCCAAT
5161 ATGGCCCTAT GCCAATATGG CTATTGGCCA GGTTCAATAC TATGTATTGG CCCTATGCCA
5221 TATAGTATTC CATATATGGG TTTTCCTATT GACGTAGATA GCCCCTCCCA ATGGGCGGTC
5281 CCATATACCA TATATGGGGC TTCCTAATAC CGCCCATAGC CACTCCCCCA TTGACGTCAA
5341 TGGTCTCTAT ATATGGTCTT TCCTATTGAC GTCATATGGG CGGTCCTATT GACGTATATG
5401 GCGCCTCCCC CATTGACGTC AATTACGGTA AATGGCCCGC CTGGCTCAAT GCCCATTGAC
5461 GTCAATAGGA CCACCCACCA TTGACGTCAA TGGGATGGCT CATTGCCCAT TCATATCCGT
5521 TCTCACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC ACTTGGCAGT ACATCAATAT
5581 CTATTAATAG TAACTTGGCA AGTACATTAC TATTGGAAGG ACGCCAGGGT ACATTGGCAG
5641 TACTCCCATT GACGTCAATG GCGGTAAATG GCCCGCGATG GCTGCCAAGT ACATCCCCAT
5701 TGACGTCAAT GGGGAGGGGC AATGACGCAA ATGGGCGTTC CATTGACGTA AATGGGCGGT
5761 AGGCGTGCCT AATGGGAGGT CTATATAAGC AATGCTCGTT TAGGGAAC
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