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pCS107

@pCS107

 

pCS107


 

编号

载体名称

北京华越洋VECT75152

pCS107

 

pCS107载体基本信息:

载体名称:

pCS107

质粒类型:

克隆载体;哺乳动物表达载体;广宿主载体;体外表达载体

高拷贝/低拷贝:

高拷贝

克隆方法:

限制性内切酶,多克隆位点

启动子:

simian CMV IE94lacT3SP6

载体大小:

4131 bp

5' 测序引物及序列:

SP6 fwdMCS1)、T3 fwdMCS2

3' 测序引物及序列:

EBV revMCS1)、SV40 polyA revMCS2

载体标签:

载体抗性:

氨苄青霉素

克隆菌株:

DH10B

表达细胞:

原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。

备注:

pCS107载体能够在多种细胞中进行高水平的瞬表达。pCS107载体 同时也能进行体外转录和翻译。

稳定性:

稳表达

组成型/诱导型:

诱导型

病毒/非病毒:

非病毒

 

pCS107载体质粒图谱和多克隆位点信息:

 

 

 


pCS107载体序列:

ORIGIN

        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag

       61 ctacttgttc tttttgcagg atcccatcga ttcgaattcg tcgacaggcc aatctggccg

      121 cggccgcaag gcctctcgag cctctcgccc tatagtgagt cgtattacgt agatccagac

      181 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc

      241 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa

      301 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag

      361 gttttttaat tcgcggcgcg ccgcggcgcc aatgcattgg gcccggtacc cagcttttgt

      421 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg

      481 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa

      541 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct

      601 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga

      661 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc

      721 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa

      781 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt

      841 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa

      901 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt

      961 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg

     1021 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc

     1081 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc

     1141 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta

     1201 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct

     1261 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc

     1321 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa

     1381 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa

     1441 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa

     1501 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt

     1561 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac

     1621 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc

     1681 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc

     1741 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata

     1801 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc

     1861 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc

     1921 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca

     1981 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa

     2041 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca

     2101 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt

     2161 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt

     2221 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg

     2281 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga

     2341 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc

     2401 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg

     2461 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag

     2521 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg

     2581 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa

     2641 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca

     2701 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga

     2761 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc

     2821 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc

     2881 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc

     2941 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg

     3001 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac

     3061 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg

     3121 cctcttcgct attacgccag tcgatcgacc atagccaatt caatatggcg tatatggact

     3181 catgccaatt caatatggtg gatctggacc tgtgccaatt caatatggcg tatatggact

     3241 cgtgccaatt caatatggtg gatctggacc ccagccaatt caatatggcg gacttggcac

     3301 catgccaatt caatatggcg gacttggcac tgtgccaact ggggaggggt ctacttggca

     3361 cggtgccaag tttgaggagg ggtcttggcc ctgtgccaag tccgccatat tgaattggca

     3421 tggtgccaat aatggcggcc atattggcta tatgccagga tcaatatata ggcaatatcc

     3481 aatatggccc tatgccaata tggctattgg ccaggttcaa tactatgtat tggccctatg

     3541 ccatatagta ttccatatat gggttttcct attgacgtag atagcccctc ccaatgggcg

     3601 gtcccatata ccatatatgg ggcttcctaa taccgcccat agccactccc ccattgacgt

     3661 caatggtctc tatatatggt ctttcctatt gacgtcatat gggcggtcct attgacgtat

     3721 atggcgcctc ccccattgac gtcaattacg gtaaatggcc cgcctggctc aatgcccatt

     3781 gacgtcaata ggaccaccca ccattgacgt caatgggatg gctcattgcc cattcatatc

     3841 cgttctcacg ccccctattg acgtcaatga cggtaaatgg cccacttggc agtacatcaa

     3901 tatctattaa tagtaacttg gcaagtacat tactattgga agtacgccag ggtacattgg

     3961 cagtactccc attgacgtca atggcggtaa atggcccgcg atggctgcca agtacatccc

     4021 cattgacgtc aatggggagg ggcaatgacg caaatgggcg ttccattgac gtaaatgggc

     4081 ggtaggcgtg cctaatggga ggtctatata agcaatgctc gtttagggaa c

//

 

pCS107其他相关广宿主载体

pMHE3Tc

pMHE7Tc

pMHE7

pMHE6

pMHE5Tc

pMHE3

pMHE2

pBBR1MCS-6

pMHE5

pBMTB-5

pBMTB-4

pBMTB-3

pBMTB-2

pBMTB-1

pBTL-5

pBTL-4

pBTL-3

pBTL-2

pBTL-1

pBTB-5

pBTB-4

pBTB-3

pBTB-2

pBTB-1

pBT-5

pBMT-5

pBMT-4

pBMT-3

pBMT-2

pBMT-1

pBT-4

pBT-2

pBT-3

pBT-1

pCS2+MT

pCS2+8CmCherry

pCS2+8CeGFP

pMMB207

pCS22+

pCS2+

pCS2-

pCS111

pCS108

pCS107

pBBR1MCS

pBBR1MCS-5

pBBR1MCS-4

pBBR1MCS-3

pBBR1MCS-2

pCSDest

pSPORT2

pBBR1-GFP

pCS2+8