pAD123
pAD123
编号 | 载体名称 |
北京华越洋VECT75424 | pAD123 |
pAD123载体基本信息：
复制子: | ori |
质粒大小: | 5952bp |
质粒标签: | GFP |
原核抗性: | Amp |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧 LB |
表达宿主: | 枯草芽孢杆菌 |
诱导方式: | IPTG诱导 |
5'测序引物: | 根据序列设计引物 |
3'测序引物: | 根据序列设计引物 |
pAD123载体图谱：
pAD123载体简介：
We constructed a promoter-trap plasmid, pAD123, for Bacillus cereus. This plasmid contains a promoterless gene that encodesa mutant version of the green fluorescent protein, GFPmut3a, that is optimized for fluorescence-activated cell sorting [Cormack, FACS-optimized mutants of the green fluorescent protein (GFP).
pAD123载体序列：
ORIGIN
1 gaattcgagc tcggtacccg gggatcctct agatttaaga aggagatata catatgagta
61 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
121 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
181 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
241 ctttcgggta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact
301 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
361 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
421 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
481 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
541 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
601 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
661 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
721 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa atctcctgca
781 ggcatgcaag cttgagtagg acaaatccgc cgagcttcga cgagattttc aggagctaag
841 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
901 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
961 cagaacaaag aatacaagaa aatatttaca aaaaatcaat ttaacaattc cttaaaacat
1021 gcaggaattg acgatttaaa caatattagc tttgaacaat tcttatctct tttcaatagc
1081 tataaattat ttaataagta agttaaggga tgcataaact gcatccctta acttgttttt
1141 cgtgtgccta ttttttgtga atcgctaaga aaccattatt atcatgacat taacctataa
1201 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
1261 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
1321 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
1381 ggcatcagag cagattgtac tgagagtgca ccatacaaaa catatttcaa cacaatacaa
1441 atgggttagt taaaaaagca ggccttctaa aggtctgctt tttttatttg attatgtaat
1501 ttttaatgcc aggatgccaa taagccataa cctcaaatgc accatttgca acctcgtcat
1561 cttcttcctc aatcttgacc agatcgccgt cctccgcatc accaagattc agctctttat
1621 gtatctcctt caaaatgcca ccgtatccaa ttaaccttcg agctgccaac gcatcatcca
1681 agtaaagcac cgtgttcaga ttgtcttcag tcaccttatt accgcgcaca caatccgtat
1741 ccttaaccgg atatttagag atttcgcgaa cagctttttg ctccatcatt gcgttccgca
1801 catcgttttc aatctgttca gcgtcaatct tagctttacc tttcactcga cgaatatcga
1861 caattggagt gtaatccaat ttcatcgcct ttttccaaag gctcgtccac tccgcctgct
1921 taatatagtt tttcccaaaa taatttttcc ttactggtat caacacatga aaatgaggat
1981 gatatgtatc ttcttcatga tttttggtaa tctctaaagc tctgaaaaat ccaagaaccg
2041 aagtttttac ttttttgtac tggaacagtt tcctaaagcc ttccatcatc gcagaaattt
2101 gtggcttcag ccgttctccc tttacatttc gaatcgtcag cgtgagaaaa atccatccgc
2161 agccgtactg tctattggct tcctctacga tcaacttatt gtgataagca atttttaacg
2221 acctgcgcca cgcacacatc ggacataacc tcactttaca aaaatgggct tgatacagtt
2281 ttaacttgcc cgtctccggg tctctcttaa acgaaagata ctctgcacaa ctaattagtt
2341 tttcagcctt tttgccatag taaggtgccc caatcttact ctctaacgct tcgtaatgct
2401 ccgccatgag gttcgtccgt ctctttttcc ccttccaatc ccgcttttta cctgttgcgg
2461 ttttatcttc gaggatgcta taatcatttt cagatgaata aatcaacaaa aaaactcctt
2521 ctgagctagt tctctagcat tctattattt tgattcgaca ccttaataat agcagaagga
2581 gtttttacct gtcaaagaac catcaaaccc ttgatacaca aggctttgac ctaattttga
2641 aaaatgatgt tgtttctata tagtatcaag ataagaaaga aaaggatttt tcgctacgct
2701 caaatccttt aaaaaaacac aaaagaccac attttttaat gtggtcttta ttcttcaact
2761 aaagcaccca ttagttcaac aaacgaaaat tggataaagt gggatatttt taaaatatat
2821 atttatgtta cagtaatatt gacttttaaa aaaggattga ttctaatgaa gaaagcagac
2881 aagtaagcct cctaaattca ctttagataa aaatttagga ggcatatcaa atgaacttta
2941 ataaaattga tttagacaat tggaagagaa aagagatatt taatcattat ttgaaccaac
3001 aaacgacttt tagtataacc acagaaattg atattagtgt tttataccga aacataaaac
3061 aagaaggata taaattttac cctgcattta ttttcttagt gacaagggtg ataaactcaa
3121 atacagcttt tagaactggt tacaatagcg acggagagtt aggttattgg gataagttag
3181 agccacttta tacaattttt gatggtgtat ctaaaacatt ctctggtatt tggactcctg
3241 taaagaatga cttcaaagag ttttatgatt tatacctttc tgatgtagag aaatataatg
3301 gttcggggaa attgtttccc aaaacaccta tacctgaaaa tgctttttct ctttctatta
3361 ttccatggac ttcatttact gggtttaact taaatatcaa taataatagt aattaccttc
3421 tacccattat tacagcagga aaattcatta ataaaggtaa ttcaatatat ttaccgctat
3481 ctttacaggt acatcattct gtttgtgatg gttatcatgc aggattgttt atgaactcta
3541 ttcaggaatt gtcagatagg cctaatgact ggcttttata atatgagata atgccgactg
3601 tactttttac agtcggtttt ctaatgtcac taacctgccc cgttagtcgc cattcgccag
3661 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
3721 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
3781 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
3841 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
3901 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
3961 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
4021 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
4081 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
4141 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
4201 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
4261 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
4321 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
4381 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
4441 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
4501 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
4561 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
4621 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
4681 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
4741 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
4801 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
4861 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
4921 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
4981 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
5041 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
5101 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
5161 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
5221 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
5281 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
5341 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
5401 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
5461 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
5521 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
5581 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
5641 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
5701 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
5761 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
5821 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
5881 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
5941 tttcgtcttc aa
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