pSE100
pSE100
编号 | 载体名称 |
北京华越洋VECT75501 | pSE100 |
pSE100载体基本信息：
复制子: | ColE1 ori |
终止子: | rrnB T2 terminator |
质粒分类: | 分枝杆菌质粒 |
质粒大小: | 5538bp |
原核抗性: | 潮霉素Hygromycin |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧，LB |
表达宿主: | 分枝杆菌 |
5'测序引物: | 根据全序列设计 |
备注: | 高拷贝 |
pSE100载体图谱:
pSE100载体简介：
hygromycin (200 mg/l for E. coli, 50 mg/l for mycobacteria). This plasmid is high copy number in E. coli and low copy number in mycobacteria.
pSE100载体序列：
ORIGIN
1 tgattagcta agcagaaggc catcctgacg gatggccttt ttgcgtttaa tactgtttaa
61 acctctagaa atattggatc gtcggcaccg tcacggccgt gggaggcggc acgatccgcg
121 acgtgatgat cggccgcatc cccacggtgc tgcgcagtga gctctacgcc atcccggcgt
181 tgatctgtgc gttcgcacgc acaggcccgg tgtgagaagg gtctctgacg agcgggagaa
241 ctccctatca gtgatagagt ttgtcctccc tatcagtgat agataggctc tgggagtacc
301 cgtgtgtacg accagcacgg catacatcat ttcgacgccg agagattcgc cgcccgaaat
361 gagcacgatc cgcatgctta attaacagct gatttaaatg gatcccccgg gctgcaggaa
421 ttcgatatca agctttagct aattaattgg ggaccctaga ggtccccttt tttattttat
481 cgataccgtc gacctcgagg gggggcccgc cggggatcat cgagccgaga acgttatcga
541 agttggtcat gtgtaatccc ctcgtttgaa ctttggatta agcgtagata cacccttgga
601 caagccagtt ggattcggag acaagcaaat tcagccttaa aaagggcgag gcctgcggtg
661 gtggaacacc gcagggcctc taaccgctcg acgcgctgca ccaaccagcc cgcgaacggc
721 tggcagccag cgtaaggcgc ggctcatcgg gcggcgttcg ccacgatgtc ctgcacttcg
781 agccaagcct cgaacacctg ctggtgtgca cgactcaccc ggttgttgac accgcgcgcg
841 gccgtgcggg ctcggtgggg cggctgtgtc gcccttgcca gcgtgagtag cgcgtacctc
901 acctcgccca acaggtcgca cacagccgat tcgtacgcca taaagccagg tgagcccacc
961 agctccgtaa gttcgggcgc tgtgtggctc gtacccgcgc attcaggcgg cagggggtct
1021 aacgggtcta aggcggcgtg tacggccgcc acagcggctc tcagcggccc ggaaacgtcc
1081 tcgaaacgac gcatgtgttc ctcctggttg gtacaggtgg ttgggggtgc tcggctgtcg
1141 ctggtgttcc accaccaggg ctcgacggga gagcggggga gtgtgcagtt gtggggtggc
1201 ccctcagcga aatatctgac ttggagctcg tgtcggacca tacaccggtg attaatcgtg
1261 gtctactacc aagcgtgagc cacgtcgccg acgaatttga gcagctctgg ctgccgtact
1321 ggccgctggc aagcgacgat ctgctcgagg ggatctaccg ccaaagccgc gcgtcggccc
1381 taggccgccg gtacatcgag gcgaacccaa cagcgctggc aaacctgctg gtcgtggacg
1441 tagaccatcc agacgcagcg ctccgagcgc tcagcgcccg ggggtcccat ccgctgccca
1501 acgcgatcgt gggcaatcgc gccaacggcc acgcacacgc agtgtgggca ctcaacgccc
1561 ctgttccacg caccgaatac gcgcggcgta agccgctcgc atacatggcg gcgtgcgccg
1621 aaggccttcg gcgcgccgtc gacggcgacc gcagttactc aggcctcatg accaaaaacc
1681 ccggccacat cgcctgggaa acggaatggc tccactcaga tctctacaca ctcagccaca
1741 tcgaggccga gctcggcgcg aacatgccac cgccgcgctg gcgtcagcag accacgtaca
1801 aagcggctcc gacgccgcta gggcggaatt gcgcactgtt cgattccgtc aggttgtggg
1861 cctatcgtcc cgccctcatg cggatctacc tgccgacccg gaacgtggac ggactcggcc
1921 gcgcgatcta tgccgagtgc cacgcgcgaa acgccgaatt cccgtgcaac gacgtgtgtc
1981 ccggaccgct accggacagc gaggtccgcg ccatcgccaa cagcatttgg cgttggatca
2041 caaccaagtc gcgcatttgg gcggacggga tcgtggtcta cgaggccaca ctcagtgcgc
2101 gccagtcggc catctcgcgg aagggcgcag cagcgcgcac ggcggcgagc acagttgcgc
2161 ggcgcgcaaa gtccgcgtca gccatggagg cattgctatg agcgacggct acagcgacgg
2221 ctacagcgac ggctacaacc ggcagccgac tgtccgcaaa aagcggcgcg tgaccgccgc
2281 cgaaggcgct cgaatcaccg gactatccga acgccacgtc gtccggctcg tggcgcagga
2341 acgcagcgag tggctcgccg agcaggctgc acgccgcgaa cgcatccgcg cctatcacga
2401 cgacgagggc cactcttggc cgcaaacggc caaacatttc gggctgcatc tggacaccgt
2461 taagcgactc ggctatcggg cgaggaaaga gcgtgcggca gaacaggaag cggctcaaaa
2521 ggcccacaac gaagccgaca atccaccgct gttctaacgc aattggggag cgggtgtcgc
2581 gggggttccg tggggggttc cgttgcaacg ggtcggacag gtaaaagtcc tggtagacgc
2641 tagttttctg gtttgggcca tgcctgtctc gttgcgtgtt tcgttgcgcc cgttttgaat
2701 accagccaga cgagacgggg ttctacgaat cttggtcgat accaagccat ttccgctgaa
2761 tatcggggag ctcaccgcca gaatcggtgg ttgtggtgat gtacgtggcg aactccgttg
2821 tagtgcctgt ggtggcatcc gtggccactc tcgttgcacg gttcgttgtg ccgttacagg
2881 ccccgttgac agctcaccga acgtagttaa aacatgctgg tcaaactagg tttaccaacg
2941 atacgagtca gctcatctag ggccagttct aggcgttgtt cgttgcgcgg ttcgttgcgc
3001 atgtttcgtg tggttgctag atggctccgc aaccacacgc ttcgaggttg agtgcttcca
3061 gcacgggcgc gatccagaag aacttcgtcg tgcgactgtc ctcgttgatc cttgccgagc
3121 tgggatggaa gctcggccga ccaccctgga ggagatgatc gaggatgcca gggcctttca
3181 cgcccgccgc tgctgagcgt ccgccgccgg gcccgcaccg ccgtcggccg gcccgctccg
3241 ggctcgcagc agcgggcttc ggcgcgggcc cggggctccc gagcgcgggc ggggctccgg
3301 gcggccgccg ggggccgggg gcggcgccgg gcggcccggg gcgtcaggcg ccgggggcgg
3361 tgtccggcgg cccccagagg aactgcgcca gttcctccgg atcggtgaag ccggagagat
3421 ccagcggggt ctcctcgaac acctcgaagt cgtgcaggaa ggtgaaggcg agcagttcgc
3481 gggcgaagtc ctcggtccgc ttccactgcg ccccgtcgag cagcgcggcc aggatctcgc
3541 ggtcgccccg gaaggcgttg agatgcagtt gcaccaggct gtagcgggag tctcccgcat
3601 agacgtcggt gaagtcgacg atcccggtga cctcggtcgc ggccaggtcc acgaagatgt
3661 tggtcccgtg caggtcgccg tggacgaacc ggggttcgcg gccggccagc agcgtgtcca
3721 cgtccggcag ccagtcctcc aggcggtcca gcagccgggg cgagaggtag ccccacccgc
3781 ggtggtcctc gacggtcgcc gcgcggcgtt cccgcagcag ttccgggaag acctcggaat
3841 ggggggtgag cacggtgttc ccggtcagcg gcaccctgtg cagccggccg agcacccggc
3901 cgagttcgcg ggccagggcg agcagcgcgt tccggtcggt cgtgccgtcc atcgcggacc
3961 gccaggtggt gccggtcatc cggctcatca ccaggtaggg ccacggccag gctccggtgc
4021 cgggccgcag ctcgccgcgg ccgaggaggc ggggcaccgg caccggggcg tccgccagga
4081 ccgcgtacgc ctccgactcc gacgcgaggc tctccggacc gcaccagtgc tcgccgaaca
4141 gcttgatcac cgggtcgggc tcgccgacca gtacggggtt ggtgctctcg ccgggcaccc
4201 gcagcaccgg cggcaccggc agcccgagct cctccagggc tcggcgggcc agcggctccc
4261 agaattcctg gtcgttccgc aggctcgcgt aggaatcatc cgaatcaata cggtcgagaa
4321 gtaacaggga ttcttgtgtc acagcggacc tctattcaca gggtacgggc cggcttaatt
4381 ccgcacggcc ggtcgcgaca cggcctgtcc gcaccgcggt caggcgttga cgatgacggg
4441 ctggtcggcc acgtccgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
4501 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
4561 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
4621 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
4681 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
4741 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
4801 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
4861 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
4921 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
4981 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
5041 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
5101 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
5161 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
5221 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
5281 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
5341 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
5401 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
5461 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
5521 aatgcagcta gaactagt
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