pFastBac-GST-N
pFastBac-GST-N
编号 | 载体名称 |
北京华越洋VECT75436 | pFastBac-GST-N |
pFastBac-GST-N载体基本信息：
启动子: | Polyhedrin |
复制子: | pUC ori，F1 ori |
终止子: | SV40 poly(A) signal |
质粒分类: | 昆虫细胞载体；杆状病毒组成型表达质粒 |
质粒大小: | 5463bp |
质粒标签: | C-3C，C-GST |
原核抗性: | 氨苄青霉素Ampicillin |
筛选标记: | 庆大霉素Gentamicin |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧，LB |
表达宿主: | 昆虫细胞 |
诱导方式: | 组成型表达，无需诱导 |
5'测序引物: | pFastbac-F: TATTCCGGATTATTCATACC |
3'测序引物: | pFastBac-R: ACAAATGTGGTATGGCTGA |
备注: | 可表达GST融合蛋白 |
pFastBac-GST-N载体图谱：
pFastBac-GST-N载体简介：
The Bac-to-Bac® Baculovirus Expression System provides a rapid and efficient method to generate recombinant baculoviruses (Ciccarone et al., 1997). This method was developed by researchers at Monsanto, and is based on site-specific transposition of an expression cassette into a baculovirus shuttle vector (bacmid) propagated in E. coli (Luckow et al., 1993). The major components of the Bac-to-Bac® Baculovirus Expression System include:
• A choice of pFastBac™ donor plasmids that allow generation of an expression construct containing the gene of interest where expression of the gene of interest is controlled by a baculovirus-specific promoter.
• An E. coli host strain, DH10Bac™, that contains a baculovirus shuttle vector (bacmid) and a helper plasmid, and allows generation of a recombinant bacmid following transposition of the pFastBac expression construct.
pFastBac-GST-N载体序列：
ORIGIN
1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
4021 ccatcgggcg cggatcccgg tccgaagcgc gcggaattca aaggcctacg tcgacgagct
4081 cactagtcgc ggccgctttc gaatctagac atatgggtac cctcgagggc agcagcctgg
4141 aagttctgtt ccaggggccc tcggatatgt cccctatact aggttattgg aaaattaagg
4201 gccttgtgca acccactcga cttcttttgg aatatcttga agaaaaatat gaagagcatt
4261 tgtatgagcg cgatgaaggt gataaatggc gaaacaaaaa gtttgaattg ggtttggagt
4321 ttcccaatct tccttattat attgatggtg atgttaaatt aacacagtct atggccatca
4381 tacgttatat agctgacaag cacaacatgt tgggtggttg tccaaaagag cgtgcagaga
4441 tttcaatgct tgaaggagcg gttttggata ttagatacgg tgtttcgaga attgcatata
4501 gtaaagactt tgaaactctc aaagttgatt ttcttagcaa gctacctgaa atgctgaaaa
4561 tgttcgaaga tcgtttatgt cataaaacat atttaaatgg tgatcatgta acccatcctg
4621 acttcatgtt gtatgacgct cttgatgttg ttttatacat ggacccaatg tgcctggatg
4681 cgttcccaaa attagtttgt tttaaaaaac gtattgaagc tatcccacaa attgataagt
4741 acttgaaatc cagcaagtat atagcatggc ctttgcaggg ctggcaagcc acgtttggtg
4801 gtggcgacca tcctccaaaa tgaaagcttg tcgagaagta ctagaggatc ataatcagcc
4861 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
4921 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
4981 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
5041 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctga tcactgcttg
5101 agcctaggag atccgaacca gataagtgaa atctagttcc aaactatttt gtcattttta
5161 attttcgtat tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta
5221 aataatcctt aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac
5281 agcggggcat ttttcttcct gttatgtttt taatcaaaca tcctgccaac tccatgtgac
5341 aaaccgtcat cttcggctac tttttctctg tcacagaatg aaaatttttc tgtcatctct
5401 tcgttattaa tgtttgtaat tgactgaata tcaacgctta tttgcagcct gaatggcgaa
5461 tgg
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pFastBac-GST-N载体其他相关的昆虫细胞载体：
