pAX01
pAX01
编号 | 载体名称 |
北京华越洋VECT75426 | pAX01 |
pAX01载体基本信息：
启动子: | Lac |
复制子: | ColE1 ori |
终止子: | T1，T2 |
质粒分类: | 广宿主系列，枯草杆菌载体 |
质粒大小: | 7781bp |
原核抗性: | Amp |
筛选标记: | erm |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧 LB |
表达宿主: | 枯草芽孢杆菌 |
诱导方式: | IPTG诱导 |
5'测序引物: | 根据序列设计引物 |
3'测序引物: | 根据序列设计引物 |
pAX01载体图谱：
pAX01载体简介：
PAX01 expression vector is constructed by MoBiTec, Bacillus subtilis, replicon plasmid ori, size 7781bp, e. (Amp); Bacillus subtilis (erythromycin resistance), can be transformed into Escherichia coli DH5 alpha, the coliform (Amp); Bacillus subtilis (erythromycin resistance) plate culture screening a large number of replication amplification culture conditions is LB, 37 C.
pAX01载体序列：
ORIGIN
1 ggggtgatgt caaagcttga aaaaacgcac gtaacaaaag caaaatttat gctccatggg
61 ggagactaca accccgatca gtggctggat cggcccgata ttttagctga cgatatcaaa
121 ctgatgaagc tttctcatac gaatacgttt tctgtcggca tttttgcatg gagcgcactt
181 gagccggagg agggcgtata tcaatttgaa tggctggatg atatttttga gcggattcac
241 agtataggcg gccgggtcat attagcaacg ccgagcggag cccgtccggc ctggctgtcg
301 caaacctatc cggaagtttt gcgcgtcaat gcctcccgcg tcaaacagct gcacggcgga
361 aggcacaacc actgcctcac atctaaagtc taccgagaaa aaacacggca catcaaccgc
421 ttattagcag aacgatacgg acatcacccg gcgctgttaa tgtggcacat ttcaaacgaa
481 tacgggggag attgccactg tgaatcgatg cggccgctct agagcaacgt tcttgccatt
541 gctgcataaa aaacgcccgg cggcaaccga gcgttctgaa ttaattaatc atcgggaaga
601 tcttcatcac cgaaacgcgg caggcagctc tagagttaac aagagtttgt agaaacgcaa
661 aaaggccatc cgtcaggatg gccttctgct tagctagagc ggcggatttg tcctactcag
721 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt
781 tcgttttatt tgatgcctca agctagagag tcattaccag atctcactgc agagatcccc
841 gggtctagag tctagggacc tctttagctc cttggaagct gtcagtagta tacctaataa
901 tttatctaca ttccctttag taacgtgtaa ctttccaaat ttacaaaagc gactcataga
961 attatttcct cccgttaaat aatagataac tattaaaaat agacaatact tgctcataag
1021 taacggtact taaattgttt actttggcgt gtttcattgc ttgatgaaac tgatttttag
1081 taaacagttg acgatattct cgattgaccc attttgaaac aaagtacgta tatagcttcc
1141 aatatttatc tggaacatct gtggtatggc gggtaagttt tattaagaca ctgtttactt
1201 ttggtttagg atgaaagcat tccgctggca gcttaagcaa ttgctgaatc gagacttgag
1261 tgtgcaagag caaccctagt gttcggtgaa tatccaaggt acgcttgtag aatccttctt
1321 caacaatcag atagatgtca gacgcatggc tttcaaaaac cactttttta ataatttgtg
1381 tgcttaaatg gtaaggaata ctcccaacaa ttttatacct ctgtttgtta gggaattgaa
1441 actgtagaat atcttggtga attaaagtga cacgagtatt cagttttaat ttttctgacg
1501 ataagttgaa tagatgactg tctaattcaa tagacgttac ctgtttactt attttagcca
1561 gtttcgtcgt taaatgccct ttacctgttc caatttcgta aacggtatcg gtttctttta
1621 aattcaattg ttttattatt tggttgagta ctttttcact cgttaaaaag ttttgagaat
1681 attttatatt tttgttcatg taatcactcc ttcttaatta caaattttta gcatctaatt
1741 taacttcaat tcctattata caaaatttta agatactgca ctatcaacac actcttaagt
1801 ttgcttctaa gtcttatttc cataacttct tttacgtttc cgccattctt tgctgtttcg
1861 atttttatga tatggtgcaa gtcagcacga acacgaaccg tcttatctcc cattatatct
1921 ttttttgcac tgattggtgt atcatttcgt ttttcttttg tgctagagga tcaattcttg
1981 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
2041 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
2101 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
2161 ataatattga aaaaggaaga gtgcggccgc ccgcgggagc tcggatccca tttccccctt
2221 tgatttttag atatcactag tttggaccat ttgtcatttc cccctttgat ttaagtgaac
2281 aagtttatcc atcaactatc ttaattgagt tagtttgttt atccaataaa ctaactttat
2341 ctcatcatat acaaaataaa tgtttatttc aatgtttttt ttagaaaatt tagttataat
2401 attagatatg atacttttaa atatctaatt caagcttcaa aaaacaccaa cttagttcgg
2461 tggataaaca aaggagtggt tattattcaa attgcagatc aagctttagt aaaaaaaatg
2521 aatcaaaaat taatattaga tgaaattttg aagaactccc ctgtctccag ggcaactctc
2581 tctgagatta caggattaaa caagtctact gtctcctctc aagtaaatac actgcttgaa
2641 aaagatttta tttttgaaat tggggcaggg caatctagag gcggcagaag acctgtaatg
2701 cttgttttta ataagaatgc aggctactcg attggtattg atataggagt cgactatctt
2761 aacggaattc taaccgactt agaaggaaat attattctcg agaagacttc tgacttgtct
2821 agttcttccg ctagtgaagt aaaagagatt ttatttgcac ttattcatgg ttttgtaacc
2881 catatgcctg agtcccctta tggtctagtc ggaataggaa tttgtgttcc aggccttgta
2941 gatcgtcatc agcaaattat tttcatgcct aacttaaatt ggaatatcaa agatttgcag
3001 tttttaattg agagtgagtt taatgttccg gtttttgttg aaaatgaagc taatgcagga
3061 gcatacggtg aaaaagtatt tggtatgaca aaaaactatg aaaacatcgt ttacatcagt
3121 attaatatcg gaattggaac tggacttgtt attaacaacg aattgtataa aggtgttcag
3181 ggtttttctg gggaaatggg tcatatgacg atagatttta atggacccaa atgcagctgt
3241 ggaaatcgag gctgttggga attatatgct tctgaaaaag cgttactggc ttcgctctct
3301 aaagaagaaa agaatatttc tcgaaaagag attgtggaac gcgcaaataa aaatgatgta
3361 gaaatgttaa atgcacttca aaactttggc ttttatatcg gaattggatt aaccaatatc
3421 cttaatacat ttgatataga agctgttatc ttgagaaatc atataattga atctcatccc
3481 attgttttaa atacgattaa aaacgaagtt tcttctagag tccattctca tttagacaat
3541 aaatgtgaac tattgccttc ttcgttagga aaaaatgcac ctgctttagg agcggtttct
3601 atcgttattg attctttttt aagtgttacc cctataagtt aggagctccc cgggacgttc
3661 ttgccattgc tgcataaaaa acgcccggcg gcaaccgagc gttctgaatt aattaatcat
3721 cgcgactgca gagatatcga tttcaagcta tatttggagt tgagcctctt gaaacggaca
3781 ccctgtatcc gaaggatcga aacgctgtca gctaccgcag ccaaatatat gaaatgaagg
3841 attatgcaac cgtgattgat gtaaagacag cttcagtgga agcggtgtat caagaagatt
3901 tttatgcgcg cacgccagcg gtcacaagcc atgagtatca gcagggcaag gcgtatttta
3961 tcggcgcgcg tttggaggat caatttcagc gtgatttcta tgagggtctg atcacagacc
4021 tgtctctctc tccagttttt ccggttcggc acggaaaagg cgtctccgta caagcgaggc
4081 aggatcagga caatgattat atttttgtca tgaatttcac ggaagaaaaa cagctggtca
4141 cgtttgatca gagtgtgaag gacataatga caggagacat attgtcaggc gacctgacga
4201 tggaaaagta tgaagtgaga attgtcgtaa acacacatta gcccatcatt cttgaagacg
4261 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
4321 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
4381 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
4441 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
4501 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
4561 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
4621 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
4681 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
4741 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
4801 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
4861 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
4921 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
4981 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
5041 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
5101 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
5161 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
5221 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
5281 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
5341 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
5401 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
5461 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
5521 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
5581 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
5641 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
5701 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
5761 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
5821 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
5881 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
5941 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
6001 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
6061 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
6121 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
6181 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
6241 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
6301 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
6361 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
6421 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
6481 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
6541 cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca
6601 tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg
6661 gccatgttaa gggcggtttt ttcctgtttg gtcacttgat gcctccgtgt aagggggaat
6721 ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta
6781 ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga
6841 tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg
6901 taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc
6961 agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg
7021 ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg
7081 gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca
7141 ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc
7201 ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca
7261 cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt
7321 gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg
7381 gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc
7441 cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag
7501 agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat
7561 ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc
7621 catccagcct cgcgtcgcga ctaagaaaat gccgtcaaat ccgctcgcca tgacttcact
7681 aacgatgcct ttgaaaatct tcaagttctt ttctactaat tcaaggcgtg tctcaccagg
7741 tttttggttt gctccggcgc aaatgcagac aatatcagga t
//
pAX01载体其他相关的枯草杆菌载体：
