pKW08-Lx
pKW08-Lx
编号 | 载体名称 |
北京华越洋VECT75503 | pKW08-Lx |
pKW08-Lx载体基本信息：
复制子: | ColE1 ori |
终止子: | rrnB T2 terminator |
质粒分类: | 分枝杆菌质粒 |
质粒大小: | 7960bp |
质粒标签: | LuxA，LuxB |
原核抗性: | 潮霉素Hygromycin |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧，LB |
表达宿主: | 分枝杆菌 |
5'测序引物: | 根据全序列设计 |
pKW08-Lx载体图谱：
pKW08-Lx载体序列：
ORIGIN
1 actagttcta gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
61 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
121 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
181 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
241 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
301 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
361 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
421 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
481 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
541 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
601 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
661 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
721 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
781 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
841 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
901 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
961 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
1021 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
1081 ccggacgtgg ccgaccagcc cgtcatcgtc aacgcctgac cgcggtgcgg acaggccgtg
1141 tcgcgaccgg ccgtgcggaa ttaagccggc ccgtaccctg tgaatagagg tccgctgtga
1201 cacaagaatc cctgttactt ctcgaccgta ttgattcgga tgattcctac gcgagcctgc
1261 ggaacgacca ggaattctgg gagccgctgg cccgccgagc cctggaggag ctcgggctgc
1321 cggtgccgcc ggtgctgcgg gtgcccggcg agagcaccaa ccccgtactg gtcggcgagc
1381 ccgacccggt gatcaagctg ttcggcgagc actggtgcgg tccggagagc ctcgcgtcgg
1441 agtcggaggc gtacgcggtc ctggcggacg ccccggtgcc ggtgccccgc ctcctcggcc
1501 gcggcgagct gcggcccggc accggagcct ggccgtggcc ctacctggtg atgagccgga
1561 tgaccggcac cacctggcgg tccgcgatgg acggcacgac cgaccggaac gcgctgctcg
1621 ccctggcccg cgaactcggc cgggtgctcg gccggctgca cagggtgccg ctgaccggga
1681 acaccgtgct caccccccat tccgaggtct tcccggaact gctgcgggaa cgccgcgcgg
1741 cgaccgtcga ggaccaccgc gggtggggct acctctcgcc ccggctgctg gaccgcctgg
1801 aggactggct gccggacgtg gacacgctgc tggccggccg cgaaccccgg ttcgtccacg
1861 gcgacctgca cgggaccaac atcttcgtgg acctggccgc gaccgaggtc accgggatcg
1921 tcgacttcac cgacgtctat gcgggagact cccgctacag cctggtgcaa ctgcatctca
1981 acgccttccg gggcgaccgc gagatcctgg ccgcgctgct cgacggggcg cagtggaagc
2041 ggaccgagga cttcgcccgc gaactgctcg ccttcacctt cctgcacgac ttcgaggtgt
2101 tcgaggagac cccgctggat ctctccggct tcaccgatcc ggaggaactg gcgcagttcc
2161 tctgggggcc gccggacacc gcccccggcg cctgacgccc cgggccgccc ggcgccgccc
2221 ccggcccccg gcggccgccc ggagccccgc ccgcgctcgg gagccccggg cccgcgccga
2281 agcccgctgc tgcgagcccg gagcgggccg gccgacggcg gtgcgggccc ggcggcggac
2341 gctcagcagc ggcgggcgtg aaaggccctg gcatcctcga tcatctcctc cagggtggtc
2401 ggccgagctt ccatcccagc tcggcaagga tcaacgagga cagtcgcacg acgaagttct
2461 tctggatcgc gcccgtgctg gaagcactca acctcgaagc gtgtggttgc ggagccatct
2521 agcaaccaca cgaaacatgc gcaacgaacc gcgcaacgaa caacgcctag aactggccct
2581 agatgagctg actcgtatcg ttggtaaacc tagtttgacc agcatgtttt aactacgttc
2641 ggtgagctgt caacggggcc tgtaacggca caacgaaccg tgcaacgaga gtggccacgg
2701 atgccaccac aggcactaca acggagttcg ccacgtacat caccacaacc accgattctg
2761 gcggtgagct ccccgatatt cagcggaaat ggcttggtat cgaccaagat tcgtagaacc
2821 ccgtctcgtc tggctggtat tcaaaacggg cgcaacgaaa cacgcaacga gacaggcatg
2881 gcccaaacca gaaaactagc gtctaccagg acttttacct gtccgacccg ttgcaacgga
2941 accccccacg gaacccccgc gacacccgct ccccaattgc gttagaacag cggtggattg
3001 tcggcttcgt tgtgggcctt ttgagccgct tcctgttctg ccgcacgctc tttcctcgcc
3061 cgatagccga gtcgcttaac ggtgtccaga tgcagcccga aatgtttggc cgtttgcggc
3121 caagagtggc cctcgtcgtc gtgataggcg cggatgcgtt cgcggcgtgc agcctgctcg
3181 gcgagccact cgctgcgttc ctgcgccacg agccggacga cgtggcgttc ggatagtccg
3241 gtgattcgag cgccttcggc ggcggtcacg cgccgctttt tgcggacagt cggctgccgg
3301 ttgtagccgt cgctgtagcc gtcgctgtag ccgtcgctca tagcaatgcc tccatggctg
3361 acgcggactt tgcgcgccgc gcaactgtgc tcgccgccgt gcgcgctgct gcgcccttcc
3421 gcgagatggc cgactggcgc gcactgagtg tggcctcgta gaccacgatc ccgtccgccc
3481 aaatgcgcga cttggttgtg atccaacgcc aaatgctgtt ggcgatggcg cggacctcgc
3541 tgtccggtag cggtccggga cacacgtcgt tgcacgggaa ttcggcgttt cgcgcgtggc
3601 actcggcata gatcgcgcgg ccgagtccgt ccacgttccg ggtcggcagg tagatccgca
3661 tgagggcggg acgataggcc cacaacctga cggaatcgaa cagtgcgcaa ttccgcccta
3721 gcggcgtcgg agccgctttg tacgtggtct gctgacgcca gcgcggcggt ggcatgttcg
3781 cgccgagctc ggcctcgatg tggctgagtg tgtagagatc tgagtggagc cattccgttt
3841 cccaggcgat gtggccgggg tttttggtca tgaggcctga gtaactgcgg tcgccgtcga
3901 cggcgcgccg aaggccttcg gcgcacgccg ccatgtatgc gagcggctta cgccgcgcgt
3961 attcggtgcg tggaacaggg gcgttgagtg cccacactgc gtgtgcgtgg ccgttggcgc
4021 gattgcccac gatcgcgttg ggcagcggat gggacccccg ggcgctgagc gctcggagcg
4081 ctgcgtctgg atggtctacg tccacgacca gcaggtttgc cagcgctgtt gggttcgcct
4141 cgatgtaccg gcggcctagg gccgacgcgc ggctttggcg gtagatcccc tcgagcagat
4201 cgtcgcttgc cagcggccag tacggcagcc agagctgctc aaattcgtcg gcgacgtggc
4261 tcacgcttgg tagtagacca cgattaatca ccggtgtatg gtccgacacg agctccaagt
4321 cagatatttc gctgaggggc caccccacaa ctgcacactc ccccgctctc ccgtcgagcc
4381 ctggtggtgg aacaccagcg acagccgagc acccccaacc acctgtacca accaggagga
4441 acacatgcgt cgtttcgagg acgtttccgg gccgctgaga gccgctgtgg cggccgtaca
4501 cgccgcctta gacccgttag accccctgcc gcctgaatgc gcgggtacga gccacacagc
4561 gcccgaactt acggagctgg tgggctcacc tggctttatg gcgtacgaat cggctgtgtg
4621 cgacctgttg ggcgaggtga ggtacgcgct actcacgctg gcaagggcga cacagccgcc
4681 ccaccgagcc cgcacggccg cgcgcggtgt caacaaccgg gtgagtcgtg cacaccagca
4741 ggtgttcgag gcttggctcg aagtgcagga catcgtggcg aacgccgccc gatgagccgc
4801 gccttacgct ggctgccagc cgttcgcggg ctggttggtg cagcgcgtcg agcggttaga
4861 ggccctgcgg tgttccacca ccgcaggcct cgcccttttt aaggctgaat ttgcttgtct
4921 ccgaatccaa ctggcttgtc caagggtgta tctacgctta atccaaagtt caaacgaggg
4981 gattacacat gaccaacttc gataacgttc tcggctcgat gatccccggc gggccccccc
5041 tcgaggtcga cggtatcgat aaaataaaaa aggggacctc tagggtcccc aattaattag
5101 ctaaagcttt tacgagtggt atttgacgat gttggcgttt accacatcga taaccgctct
5161 ttgctgcgct ttatcttcca tcgactcaaa agacatcaat aggtccgcgg caccacaaca
5221 ctcaatcgca actcgcgctg cctgagtact ttcttcataa gtaccgatgg cattttctga
5281 caacagctct cccatttttt gctcaaagtc ggtatttgag taagattcac ggacaaactc
5341 ttccaaatac acgcgagctt ctgcccttgc tgcttcacca tctacatttt ggttgaccag
5401 cagcgtcagc ttgtgtcgaa cctgactaac atcgacacca tgtgcctgag caacttcgtg
5461 gtacaaaccg gcgtattctt ttctttgagc gtttgagtcg tcccatctaa acacgagtgg
5521 aagccctaac ttagccgccc attcaaccac ttctttgctc gtcgcattca caaattgcgc
5581 aggaccgcct tcagtgaacg cgtgtgggtt aacggagatt ttaggaaaac tataaaaatc
5641 attgtttgga tggcagtacc cagtagtgaa tgaatcattg atgatcttgt gacactcact
5701 gaacaactga aactgagaat ccgttggtcg attaaagaag cgcatatctg cactcttttc
5761 acaatcacta aagccaaagg caaaacggcc ttcactcatt tggtcaagta gacacgcttc
5821 ttccgccaca cgtactggat gatgcgtggt aatgacgtga ttcaacgaag ccactttggc
5881 gttctttgtc ataccaagta aaaaaccagc cactgttagt ggggcaccaa ccacaccatt
5941 gttcgagaaa tggttttcgt aaacagccaa cgtgtcaaac ttcaactgat ctacgtaatg
6001 tgcggtatct aacatttctt cgatgacttg atcagaagaa cgctttgagt tcataaaatt
6061 gaggaagaat aatccaaatt tcatgtctct ttccttttag aaaatattaa ttactgtttt
6121 tctttgagat atggcatcac atcagactgg aatagcttca tagatgcgat aatttcttct
6181 tcagaaccgt ttgcttcaaa accacaacaa atattgtcaa tacccgtcgc atcaatatct
6241 tgctggataa tcgcgataca ctcttcaggc gtccctactg ggttgatttc gtagctgtaa
6301 tcaattcggc gattggtgtc tttgtggcct ttcaacacaa aatcacgcca ttgaccttta
6361 ttgaagtcgt aaccttttgt ttggtcagag tcgtcaaaaa tcttggtggc attcacgtat
6421 gagtcgtacc aatggcccaa gaagttgcgg caaatatctt tggctctatt tgagtcatga
6481 tcgacggagg tgatgtaaga caaacagtgg tcaatcttag tcacatcgta gccatgttca
6541 gtcgcgactt cgttgtaaag atcaagctgc gctttcttct cgtgagtgtt gatgatccag
6601 cttagaatca ttggtaggcc acgctctgca gcccattctg tcgttgatgc tgactccgcg
6661 acgacataaa caggagcacc accttgtgtg taagccgatg gattcagttg gattttcggg
6721 aacttaatat gttcgttatc cgccgcgata tagccttcat tgaagccttc tttcatcaag
6781 tcataccaac agtccattaa ggctcggctg ttatccatgt ctgtaccaaa gacacgaaaa
6841 tctttatcgt acaaaccgcg acaaatacca aaacggaatc gtccttttga catttgatcc
6901 agtaggttta cgtcttctgc ttgtcgaacc ggatgggcag tcggcaatac gatagctgca
6961 gtgccaacgt tgagcgtttc tgtcgcacct aataggtgtg cggcagcaac ataaggattc
7021 cctaacaacc caaattcagt gaagtggtgc tctagcaacc aaacggtgtc gaagccacaa
7081 ccttcagacg ctttgcccag attaaccaat cgcttcatca cttcggtctg agatagctca
7141 ggtggctgat aagtgaggag gaagtttcca aatttcataa catttcctcc ggatcctgtc
7201 aggattccac gatgagagta gcgagcagtc agatgagagt agcgagcagt cactttatct
7261 aagataaagt tatcgatgat aaagtgcaac tatgactcac caccggaagc gtctcgaccg
7321 cacgtccgtg ttgctcgggg cccggcaggt gctcgacgag actgggctcg atggcttcac
7381 cactcgcgcg ctggcggctc acttacaggt acagcagccc ggcctctact ggcacttccg
7441 cacgaaggcc gaactcctcg cagcgctggc gagcgacgtg cttgaccgag agcatcacgc
7501 ttccttcccc gagtccgacg agaaatggga cgcatttctc ttgcgaaacg caagaagttt
7561 ccgccacgca ctacacgcgg tccgtgacgg tgcgcgtctc cacgctgagc accaccgcag
7621 accatccggc gacgacaccg acgaaggagc tgaggcttcc ggtcagcaag tcgggcttct
7681 cgtgtctcag ggattcgatg aacagacagc catcagcatg ctcatcgcgg tcagccgata
7741 caccgtcggt gtcgtactgg aggagcaaac cgcagaggtc ggcaatgcga gcgttcctga
7801 acgcgaccgg gaattcgatt tcggcctgac agcactgatc gacggattct ctcactcccg
7861 cacagcgtca ttgacctcga gcgaatcgtg atctagaggt ttaaacagta ttaaacgcaa
7921 aaaggccatc cgtcaggatg gccttctgct tagctaatca
//
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