pLysS

pLysS

pLysS载体基本信息：

pLysS载体图谱：

pLysS载体简介：

    pLysS and pLysE are 4886bp plasmids constructed by insertion of the T7 lysozyme gene into the BamH I site of pACYC184 (1, 2). These plasmids are not cloning vectors; they are used in λDE3 lysogenic hosts to suppress basal expression from the T7 promoter by producing T7 lysozyme, a natural inhibitor of T7 RNA polymerase. The two plasmids differ only by the orientation of the T7 lysozyme gene. In pLysS the T7 lysozyme coding sequence is in the antisense orientation relative to the tet promoter, so only a small amount of T7 lysozyme is produced. In pLysE large amounts of T7 lysozyme are produced from the tet promoter. The construct also contains the weak T7 φ3.8 promoter immediately following the lysozyme gene. The p15A origin of replication is compatible with those found in pBR322- and pUC-derived plasmids.Unique sites are shown on the circle map.
    1. Studier , F.W. (1991) J. Mol. Biol. 219, 37-44.
    2. Chang, A.C.Y. and Cohen, S.N. (1978) J. Bacteriol. 134, 1141.

pLysS载体序列：

ORIGIN

        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt

       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt

      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga

      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga

      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt

      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc

      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat

      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt

      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg

      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact

      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa

      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc

      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc

      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa

      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc

      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttcccctg gcggctccct

      961 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg

     1021 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact

     1081 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg

     1141 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta

     1201 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt

     1261 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc

     1321 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca

     1381 aaacgatctc aagaagatca tcttattaat cagataaaat atttctagat ttcagtgcaa

     1441 tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca

     1501 tgtttgacag cttatcatcg ataagcttta atgcggtagt ttatcacagt taaattgcta

     1561 acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt

     1621 caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga

     1681 tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt

     1741 gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc

     1801 agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc

     1861 cgtcctgtgg atccggccca ttggctgcct cccacacttg gatatgcctc ctcggagcct

     1921 tatagaattg tttataagac ttgcgcatta tttgacctcc aatgcgaaca aagggaaacc

     1981 gctgtggtct ccctttagtg agttcaatta attatccacg gtcagaagtg accagttcgt

     2041 tcttctccca ccaacgctta aggtcgaacg aagggcaagc cttcggcgcc acctcatgat

     2101 gggcgcgaag accagcgcct tcgtacttag ccagcagtgt gacaagcagt gagcgaaggg

     2161 attgcatttg ggctggcgta aagttagcgt cgaacttacc tttatcgtcg ataccaccaa

     2221 caaggcagac gccgatagag ttgtggttgt aacccttagc gtgagagcct acagccatct

     2281 catctcgtcc tgcctccaca gtaccgtctc gcttgatgat aaagtggtat cccacatcga

     2341 gccaaccctg ctctttgtgc cactggcgaa tctcacggac accaacattc tgacttggct

     2401 tggtagccga gcagtgaaca aagattgcgt cagtagattc acgttgttta aactgtacac

     2461 gagccattat ttctttcctc ctttcctttt taatctatca aaggggaccc ggatcctcta

     2521 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat

     2581 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt

     2641 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca

     2701 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct

     2761 aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt

     2821 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt

     2881 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg

     2941 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc

     3001 cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat

     3061 tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg

     3121 ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt

     3181 gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct

     3241 cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg cgatttatgc

     3301 cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt

     3361 ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc

     3421 cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg

     3481 agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc

     3541 agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc

     3601 gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat

     3661 gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga

     3721 gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtcc

     3781 cctacgtgct gctgaagttg cccgcaacag agagtggaac caaccggtga taccacgata

     3841 ctatgactga gagtcaacgc catgagcggc ctcatttctt attctgagtt acaacagtcc

     3901 gcaccgctgt ccggtagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt

     3961 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgcc caacagtccc

     4021 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgccctgcac cattatgttc

     4081 cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg

     4141 aagcgctaac cgtttttatc aggctctggg aggcagaata aatgatcata tcgtcaatta

     4201 ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag cacacggtca

     4261 cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac

     4321 gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg

     4381 cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata actgccttaa

     4441 aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg

     4501 ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc

     4561 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata

     4621 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac

     4681 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct

     4741 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa

     4801 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc

     4861 agctcaccgt ctttcattgc catacg

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