pRK415
pRK415
编号 | 载体名称 |
北京华越洋VECT75508 | pRK415 |
pRK415载体基本信息：
启动子: | Lac/lac |
复制子: | oriV |
质粒分类: | 广宿主质粒 |
质粒大小: | 10690bp |
质粒标签: | traJ，LacZ |
原核抗性: | 四环素Tetracyclin |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧，LB |
5'测序引物: | M13R:CAGGAAACAGCTATGACC |
3'测序引物: | M13F:TGTAAAACGACGGCCAGT |
pRK415载体图谱：
pRK415载体序列：
ORIGIN
1 ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga
61 ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc
121 ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa
181 gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg
241 gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca
301 ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag
361 tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg
421 taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat
481 cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg
541 tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg
601 ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg
661 gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcggaaaggc gctcttccgc
721 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
781 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
841 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
901 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
961 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
1021 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
1081 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
1141 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
1201 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgagctc ggtacccggg
1261 gatcctctag agtcgacctg caggcatgca agcttggcgt aatcatggtc atagctgttt
1321 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
1381 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
1441 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
1501 gggagaggcg gtttgcgtat tgggcgctcg gtcttgcctt gctcgtcggt gatgtacttc
1561 accagctccg cgaagtcgct cttcttgatg gagcgcatgg ggacgtgctt ggcaatcacg
1621 cgcacccccc ggccgtttta gcggctaaaa aagtcatggc tctgccctcg ggcggaccac
1681 gcccatcatg accttgccaa gctcgtcctg cttctcttcg atcttcgcca gcagggcgag
1741 gatcgtggca tcaccgaacc gcgccgtgcg cgggtcgtcg gtgagccaga gtttcagcag
1801 gccgcccagg cggcccaggt cgccattgat gcgggccagc tcgcggacgt gctcatagtc
1861 cacgacgccc gtgattttgt agccctggcc gacggccagc aggtaggccg acaggctcat
1921 gccggccgcc gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc agtacacctt
1981 gataggtggg ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc
2041 tgttacgccg gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag
2101 gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat
2161 cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa
2221 tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa
2281 gcagggttat gcagcggaaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
2341 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
2401 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
2461 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
2521 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
2581 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
2641 gcagcgagtc agtgagcgag gaagcggaag agcgccagaa ggccgccaga gaggccgagc
2701 gcggccgtga ggcttggacg ctagggcagg gcatgaaaaa gcccgtagcg ggctgctacg
2761 ggcgtctgac gcggtggaaa gggggagggg atgttgtcta catggctctg ctgtagtgag
2821 tgggttgcgc tccggcagcg gtcctgatca atcgtcaccc tttctcggtc cttcaacgtt
2881 cctgacaacg agcctccttt tcgccaatcc atcgacaatc accgcgagtc cctgctcgaa
2941 cgctgcgtcc ggaccggctt cgtcgaaggc gtctatcgcg gcccgcaaca gcggcgagag
3001 cggagcctgt tcaacggtgc cgccgcgctc gccggcatcg ctgtcgccgg cctgctcctc
3061 aagcacggcc ccaacagtga agtagctgat tgtcatcagc gcattgacgg cgtccccggc
3121 cgaaaaaccc gcctcgcaga ggaagcgaag ctgcgcgtcg gccgtttcca tctgcggtgc
3181 gcccggtcgc gtgccggcat ggatgcgcgc gccatcgcgg taggcgagca gcgcctgcct
3241 gaagctgcgg gcattcccga tcagaaatga gcgccagtcg tcgtcggctc tcggcaccga
3301 atgcgtatga ttctccgcca gcatggcttc ggccagtgcg tcgagcagcg cccgcttgtt
3361 cctgaagtgc cagtaaagcg ccggctgctg aacccccaac cgttccgcca gtttgcgtgt
3421 cgtcagaccg tctacgccga cctcgttcaa caggtccagg gcggcacgga tcactgtatt
3481 cggctgcaac tttgtcatgc ttgacacttt atcactgata aacataatat gtccaccaac
3541 ttatcagtga taaagaatcc gcgcgttcaa tcggaccagc ggaggctggt ccggaggcca
3601 gacatgaaac ccaacatacc cctgatcgta attctgagca ctgtcgcgct cgacgctgtc
3661 ggcatcggcc tgattatgcc ggtgctgccg ggcctcctgc gcgatctggt tcactcgaac
3721 gacgtcaccg cccactatgg cattctgctg gcgctgtatg cgttggtgca atttgcctgc
3781 gcacctgtgc tgggcgcgct gtcggatcgt ttcgggcggc ggccaatctt gctcgtctcg
3841 ctggccggcg ccactgtcga ctacgccatc atggcgacag cgcctttcct ttgggttctc
3901 tatatcgggc ggatcgtggc cggcatcacc ggggcgactg gggcggtagc cggcgcttat
3961 attgccgata tcactgatgg cgatgagcgc gcgcggcact tcggcttcat gagcgcctgt
4021 ttcgggttcg ggatggtcgc gggacctgtg ctcggtgggc tgatgggcgg tttctccccc
4081 cacgctccgt tcttcgccgc ggcagccttg aacggcctca atttcctgac gggctgtttc
4141 cttttgccgg agtcgcacaa aggcgaacgc cggccgttac gccgggaggc tctcaacccg
4201 ctcgcttcgt tccggtgggc ccggggcatg accgtcgtcg ccgccctgat ggcggtcttc
4261 ttcatcatgc aacttgtcgg acaggtgccg gccgcgcttt gggtcatttt cggcgaggat
4321 cgctttcact gggacgcgac cacgatcggc atttcgcttg ccgcatttgg cattctgcat
4381 tcactcgccc aggcaatgat caccggccct gtagccgccc ggctcggcga aaggcgggca
4441 ctcatgctcg gaatgattgc cgacggcaca ggctacatcc tgcttgcctt cgcgacacgg
4501 ggatggatgg cgttcccgat catggtcctg cttgcttcgg gtggcatcgg aatgccggcg
4561 ctgcaagcaa tgttgtccag gcaggtggat gaggaacgtc aggggcagct gcaaggctca
4621 ctggcggcgc tcaccagcct gacctcgatc gtcggacccc tcctcttcac ggcgatctat
4681 gcggcttcta taacaacgtg gaacgggtgg gcatggattg caggcgctgc cctctacttg
4741 ctctgcctgc cggcgctgcg tcgcgggctt tggagcggcg cagggcaacg agccgatcgc
4801 tgatcgtgga aacgataggc ctatgccatg cgggtcaagg cgacttccgg caagctatac
4861 gcgccctagg agtgcggttg gaacgttggc ccagccagat actcccgatc acgagcagga
4921 cgccgatgat ttgaagcgca ctcagcgtct gatccaagaa caaccatcct agcaacacgg
4981 cggtccccgg gctgagaaag cccagtaagg aaacaactgt aggttcgagt cgcgagatcc
5041 cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac gccaggccga
5101 gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct actggaacga
5161 gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc acgggaggtt
5221 gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc aggcccgctg
5281 cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc acgcccgcag
5341 ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc agagcggcag
5401 agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc cgcccggcag
5461 gcggtagacc gaaataaaca acaagctcca gaatagcgaa atattaagtg cgccgaggat
5521 gaagatgcgc atccaccaga ttcccgttgg aatctgtcgg acgatcatca cgagcaataa
5581 acccgccggc aacgcccgca gcagcatacc ggcgacccct cggcctcgct gttcgggctc
5641 cacgaaaacg ccggacagat gcgccttgtg agcgtccttg gggccgtcct cctgtttgaa
5701 gaccgacagc ccaatgatct cgccgtcgat gtaggcgccg aatgccacgg catctcgcaa
5761 ccgttcagcg aacgcctcca tgggcttttt ctcctcgtgc tcgtaaacgg acccgaacat
5821 ctctggagct ttcttcaggg ccgacaatcg gatctcgcgg aaatcctgca cgtcggccgc
5881 tccaagccgt cgaatctgag ccttaatcac aattgtcaat tttaatcctc tgtttatcgg
5941 cagttcgtag agcgcgccgt gcgcccgagc gatactgagc gaagcaagtg cgtcgagcag
6001 tgcccgcttg ttcctgaaat gccagtaaag cgctggctgc tgaaccccca gccggaactg
6061 accccacaag gccctagcgt ttgcaatgca ccaggtcatc attgacccag gcgtgttcca
6121 ccaggccgct gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca
6181 cgcgggtgga atccgatccg cacatgaggc ggaaggtttc cagcttgagc gggtacggct
6241 cccggtgcga gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact
6301 tctcccatat gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga
6361 tcaggacctg gcaacgggac gttttcttgc cacggtccag gacgcggaag cggtgcagca
6421 gcgacaccga ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta
6481 ggcgcgacag gcattcctcg gccttcgtgt aataccggcc attgatcgac cagcccaggt
6541 cctggcaaag ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt
6601 actccaacac ctgctgccac accagttcgt catcgtcggc ccgcagctcg acgccggtgt
6661 aggtgatctt cacgtccttg ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg
6721 ggattttctt gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc
6781 gcatcgtgtc cggccacggc gcaatatcga acaaggaaag ctgcatttcc ttgatctgct
6841 gcttcgtgtg tttcagcaac gcggcctgct tggcctcgct gacctgtttt gccaggtcct
6901 cgccggcggt ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact
6961 tcgccaaacc tgccgcctcc tgttcgagac gacgcgaacg ctccacggcg gccgatggcg
7021 cgggcagggc agggggagcc agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct
7081 ggaccatcga gccgacggac tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg
7141 cgatggtttc ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct
7201 tccggtcaaa cgtccgattc attcaccctc cttgcgggat tgccccgact cacgccgggg
7261 caatgtgccc ttattcctga tttgacccgc ctggtgcctt ggtgtccaga taatccacct
7321 tatcggcaat gaagtcggtc ccgtagaccg tctggccgtc cttctcgtac ttggtattcc
7381 gaatcttgcc ctgcacgaat accagcgacc ccttgcccaa atacttgccg tgggcctcgg
7441 cctgagagcc aaaacacttg atgcggaaga agtcggtgcg ctcctgcttg tcgccggcat
7501 cgttgcgcca ctcttcatta accgctatat cgaaaattgc ttgcggcttg ttagaattgc
7561 catgacgtac ctcggtgtca cgggtaagat taccgataaa ctggaactga ttatggctca
7621 tatcgaaagt ctccttgaga aaggagactc tagtttagct aaacattggt tccgctgtca
7681 agaactttag cggctaaaat tttgcgggcc gcgaccaaag gtgcgagggg cggcttccgc
7741 tgtgtacaac cagatatttt tcaccaacat ccttcgtctg ctcgatgagc ggggcatgac
7801 gaaacatgag ctgtcggaga gggcaggggt ttcaatttcg tttttatcag acttaaccaa
7861 cggtaaggcc aacccctcgt tgaaggtgat ggaggccatt gccgacgccc tggaaactcc
7921 cctacctctt ctcctggagt ccaccgacct tgaccgcgag gcactcgcgg agattgcggg
7981 tcatcctttc aagagcagcg tgccgcccgg atacgaacgc atcagtgtgg ttttgccgtc
8041 acataaggcg tttatcgtaa agaaatgggg cgacgacacc cgaaaaaagc tgcgtggaag
8101 gctctgacgc caagggttag ggcttgcact tccttcttta gccgctaaaa cggccccttc
8161 tctgcgggcc gtcggctcgc gcatcatatc gacatcctca acggaagccg tgccgcgaat
8221 ggcatcgggc gggtgcgctt tgacagttgt tttctatcag aacccctacg tcgtgcggtt
8281 cgattagctg tttgtcttgc aggctaaaca ctttcggtat atcgtttgcc tgtgcgataa
8341 tgttgctaat gatttgttgc gtaggggtta ctgaaaagtg agcgggaaag aagagtttca
8401 gaccatcaag gagcgggcca agcgcaagct ggaacgcgac atgggtgcgg acctgttggc
8461 cgcgctcaac gacccgaaaa ccgttgaagt catgctcaac gcggacggca aggtgtggca
8521 cgaacgcctt ggcgagccga tgcggtacat ctgcgacatg cggcccagcc agtcgcaggc
8581 gattatagaa acggtggccg gattccacgg caaagaggtc acgcggcatt cgcccatcct
8641 ggaaggcgag ttccccttgg atggcagccg ctttgccggc caattgccgc cggtcgtggc
8701 cgcgccaacc tttgcgatcc gcaagcgcgc ggtcgccatc ttcacgctgg aacagtacgt
8761 cgaggcgggc atcatgaccc gcgagcaata cgaggtcatt aaaagcgccg tgattgatga
8821 tatagcggcc cggctgctcc tggttctcgc gcaccgaaat gggtgacttc accccgcgct
8881 ctttgatcgt ggcaccgatt tccgcgatgc tctccgggga aaagccgggg ttgtcggccg
8941 tccgcggctg atgcggatct tcgtcgatca ggtccaggtc cagctcgata gggccggaac
9001 cgccctgaga cgccgcagga gcgtccagga ggctcgacag gtcgccgatg ctatccaacc
9061 ccaggccgga cggctgcgcc gcgcctgcgg cttcctgagc ggccgcagcg gtgtttttct
9121 tggtggtctt ggcttgagcc gcagtcattg ggaaatctcc atcttcgtga acacgtaatc
9181 agccagggcg cgaacctctt tcgatgcctt gcgcgcggcc gttttcttga tcttccagac
9241 cggcacaccg gatgcgaggg catcggcgat gctgctgcgc aggccaacgg tggccggaat
9301 catcatcttg gggtacgcgg ccagcagctc ggcttggtgg cgcgcgtggc gcggattccg
9361 cgcatcgacc ttgctgggca ccatgccaag gaattgcagc ttggcgttct tctggcgcac
9421 gttcgcaatg gtcgtgacca tcttcttgat gccctggatg ctgtacgcct caagctcgat
9481 gggggacagc acatagtcgg ccgcgaagag ggcggccgcc aggccgacgc caagggtcgg
9541 ggccgtgtcg atcaggcaca cgtcgaagcc ttggttcgcc agggccttga tgttcgcccc
9601 gaacagctcg cgggcgtcgt ccagcgacag ccgttcggcg ttcgccagta ccgggttgga
9661 ctcgatgagg gcgaggcgcg cggcctggcc gtcgccggct gcgggtgcgg tttcggtcca
9721 gccgccggca gggacagcgc cgaacagctt gcttgcatgc aggccggtag caaagtcctt
9781 gagcgtgtag gacgcattgc cctgggggtc caggtcgatc acggcaaccc gcaagccgcg
9841 ctcgaaaaag tcgaaggcaa gatgcacaag ggtcgaagtc ttgccgacgc cgcctttctg
9901 gttggccgtg accaaagttt tcatcgtttg gtttcctgtt ttttcttggc gtccgcttcc
9961 cacttccgga cgatgtacgc ctgatgttcc ggcagaaccg ccgttacccg cgcgtacccc
10021 tcgggcaagt tcttgtcctc gaacgcggcc cacacgcgat gcaccgcttg cgacactgcg
10081 cccctggtca gtcccagcga cgttgcgaac gtcgcctgtg gcttcccatc gactaagacg
10141 ccccgcgcta tctcgatggt ctgctgcccc acttccagcc cctggatcgc ctcctggaac
10201 tggctttcgg taagccgttt cttcatggat aacacccata atttgctccg cgccttggtt
10261 gaacatagcg gtgacagccg ccagcacatg agagaagttt agctaaacat ttctcgcacg
10321 tcaacacctt tagccgctaa aactcgtcct tggcgtaaca aaacaaaagc ccggaaaccg
10381 ggctttcgtc tcttgccgct tatggctctg cacccggctc catcaccaac aggtcgcgca
10441 cgcgcttcac tcggttgcgg atcgacactg ccagcccaac aaagccggtt gccgccgccg
10501 ccaggatcgc gccgatgatg ccggccacac cggccatcgc ccaccaggtc gccgccctca
10561 ctgcccggca cctggtcgct gaatgtcgat gccagcacct gcggcacgtc aatgcttccg
10621 ggcgtcgcgc tcgggctgat cgcccatccc gttactgccc cgatcccggc aatggcaagg
10681 actgccagcg
//
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