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首页    载体及质粒    pISRE-TA-Luc
pISRE-TA-Luc载体图谱

pISRE-TA-Luc

pISRE-TA-Luc

pISRE-TA-Luc


 

编号

载体名称

北京华越洋VECT75321

pISRE-TA-Luc

 

pISRE-TA-Luc载体基本信息:

载体名称:

pISRE-TA-Luc

质粒类型:

信号通路报告系统载体

高拷贝/低拷贝:

--

启动子:

--

克隆方法:

多克隆位点,限制性内切酶

载体大小:

4.9kb

5'   测序引物及序列:

--

3'   测序引物及序列:

--

载体标签:

--

载体抗性:

Ampicillin

筛选标记:

--

备注:

--

稳定性:

--

组成型:

--

病毒/非病毒:

--

 

pISRE-TA-Luc载体质粒图谱和多克隆位点信息:

 

pISRE-TA-Luc载体简介:

 

pISRE-TA-Luc is designed for monitoring the induction of the STAT1 and STAT2 components of Jak/STAT-mediated signal

transduction pathways. Signaling molecules, including type I(IFN- and -) and type II (IFN-) interferons (1), induce

signalling by binding receptors and causing receptor dimerization at the cell surface. This dimerization causes the receptor

itself to be phosphorylated and act as a docking site for transcription factors, including STAT1 and STAT2. The STAT proteins

are then phosphorylated, dimerize and translocate to the nucleus, where the STAT1 and STAT2 heterodimer regulates transcription by binding to the IFN-stimulated response element (ISRE; 2). pISRE-TA-Luc contains five copies of the ISRE enhancer element,

located upstream of the minimal TA promoter, the TATA box from the herpes simplex virus thymidine kinase promoter (PTA).

Located downstream of the PTA is the firefly luciferase gene (luc). Upon binding of the STAT1 and STAT2 heterodimer to the

cis-acting ISRE enhancer element, transcription is induced and the reporter gene is activated.

The luciferase coding sequence is followed by the SV40 late polyadenylation signal to ensure proper, efficient processing of

the luciferase transcript in eukaryotic cells. A synthetic transcription blocker (TB) is located upstream of the cis-acting

enhancer element. It is composed of adjacent polyadenylation and transcription pause sites for blocking nonspecific

transcription (3). The vector backbone also contains an f1 origin for single-stranded DNA production, a pUC origin of

replication, and an ampicillin resistance gene for propagation and selection in E. coli.

 

pISRE-TA-Luc载体其他相关的其他类型载体:

TOPFlash

pISRE-TA-Luc

TEV protease

pIL253

SuperTopFlash

pIJ8860

pZsGreen1-DR

pHY300PLK

pZsGreen1-1

pHY300

pZL507

pHT304

pYCT

pHT43

pXMJ19

pHT01

pVLT33

pHSE-Luc

pUG6

pHIS1525

pTimer-1

pHGF9050

pTimer

pHD_v2

pTA-Luc

pHcRed1-DR

pTALETF_v2 (NN)

pHcRed1-1

pTALETF_v2 (NI)

pHCMC05

pTALETF_v2 (NG)

pHCMC04

pTALETF_v2 (HD)

pGRE-luc

pTALEN_v2 (NN)

pGFP315

pTALEN_v2 (NI)

pGFP22

pTALEN_v2 (NG)

pGEM-3

pTALEN_v2 (HD)

pGAS-TA-Luc

pSRE-Luc

pE2F-TA-Luc

pSH65

pDsRed-Express-DR

pSH63

pDsRed-Express2-1

pSH47

pDsRed-Express-1

pSG1154

pDsRed2-1

pset152

pDG1363

pRK2013

pCSN44

pQE-TriSystem

pDD-tdTomato Reporter

pOS7001

pCRE-Luc

pNZ8048

pCR-Blunt

pNN_v2

pCMV-Raf-1

pNI v2

pCMV-p53

pNG_v2

pCMV-IκBα

pMUTIN4

pCMV-CREB

pMG36e

pCaspase3-sensor

pMetLuc2-Reporter

pCantab 5E

pMetLuc2-Control

pBT2

pmCherry-1

pBR322

pMA09-J

pBIG2R-HPH2

pMA09-H

pAP-1-Luc

pMA5MCS

pAN8-1

pKOR1

pAD43-25

pkc1139

pAD43-25

pK18mobSacB

pAcGFP1-1

pJLA502

HRV 3C protease

pIκB-EGFP

GG499

pAN7-1

p53-Luc

pAP1(PMA)-TA-Luc