北京华越洋生物
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7*24h全国热线:
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首页    载体及质粒    ​pCR-Blunt
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​pCR-Blunt

pCR-Blunt

pCR-Blunt


 

编号

载体名称

北京华越洋VECT75311

pCR-Blunt

 

pCR-Blunt载体基本信息:

载体名称:

pCR-Blunt

质粒类型:

原核表达载体;克隆载体

高拷贝/低拷贝:

--

启动子:

--

克隆方法:

多克隆位点,限制性内切酶

载体大小:

4.6   kb

5'   测序引物及序列:

--

3'   测序引物及序列:

--

载体标签:

--

载体抗性:

Kanamycin

筛选标记:

Zeocin

备注:

--

稳定性:

--

组成型:

--

病毒/非病毒:

--

 

pCR-Blunt载体序列:

AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTT

 

TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAG

 

GCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAA

 

ACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACGCGTTAGAATACTCAAGCTATGCATCAAGCT

 

TGGTACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCAGGCCTGAATTCTGCAGATA

 

TCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCAATTCGCCCTATAGTGAGTCGTATTAC

 

AATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTG

 

CAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTT

 

GCGCAGCCTATACGTACGGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTG

 

GATGTACAGAGTGATATTATTGACACGCCGGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGC

 

TGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGAC

 

CACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAAT

 

GACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCATGAGATTATCAAAAAGGA

 

TCTTCACCTAGATCCTTTTCACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAG

 

CTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACA

 

TGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTG

 

GTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGG

 

ATCAAGCTCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGT

 

TCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATG

 

CCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCT

 

GAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTG

 

CTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGT

 

CATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGA

 

TCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCC

 

GGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGC

 

TCAAGGCGAGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCAT

 

GGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGAC

 

ATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTT

 

ACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAATTAT

 

TAACGCTTACAATTTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATCAGG

 

TGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT

 

CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAGCACGTGAGGAGGGCCACCATGGCCAAGT

 

TGACCAGTGCCGTTCCGGTGCTCACCGCGCGCGACGTCGCCGGAGCGGTCGAGTTCTGGACCGACCGGCT

 

CGGGTTCTCCCGGGACTTCGTGGAGGACGACTTCGCCGGTGTGGTCCGGGACGACGTGACCCTGTTCATC

 

AGCGCGGTCCAGGACCAGGTGGTGCCGGACAACACCCTGGCCTGGGTGTGGGTGCGCGGCCTGGACGAGC

 

TGTACGCCGAGTGGTCGGAGGTCGTGTCCACGAACTTCCGGGACGCCTCCGGGCCGGCCATGACCGAGAT

 

CGGCGAGCAGCCGTGGGGGCGGGAGTTCGCCCTGCGCGACCCGGCCGGCAACTGCGTGCACTTCGTGGCC

 

GAGGAGCAGGACTGACACGTGCTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTG

 

ATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGAT

 

CAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTA

 

CCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAG

 

CGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACC

 

GCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACC

 

GGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACAC

 

AGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCAC

 

GCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGG

 

GAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTC

 

GATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTT

 

CCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGT

 

ATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCG

 

AGGAAGCGGAAG

 

pCR-Blunt载体其他相关的其他类型载体:

TOPFlash

pISRE-TA-Luc

TEV protease

pIL253

SuperTopFlash

pIJ8860

pZsGreen1-DR

pHY300PLK

pZsGreen1-1

pHY300

pZL507

pHT304

pYCT

pHT43

pXMJ19

pHT01

pVLT33

pHSE-Luc

pUG6

pHIS1525

pTimer-1

pHGF9050

pTimer

pHD_v2

pTA-Luc

pHcRed1-DR

pTALETF_v2 (NN)

pHcRed1-1

pTALETF_v2 (NI)

pHCMC05

pTALETF_v2 (NG)

pHCMC04

pTALETF_v2 (HD)

pGRE-luc

pTALEN_v2 (NN)

pGFP315

pTALEN_v2 (NI)

pGFP22

pTALEN_v2 (NG)

pGEM-3

pTALEN_v2 (HD)

pGAS-TA-Luc

pSRE-Luc

pE2F-TA-Luc

pSH65

pDsRed-Express-DR

pSH63

pDsRed-Express2-1

pSH47

pDsRed-Express-1

pSG1154

pDsRed2-1

pset152

pDG1363

pRK2013

pCSN44

pQE-TriSystem

pDD-tdTomato Reporter

pOS7001

pCRE-Luc

pNZ8048

pCR-Blunt

pNN_v2

pCMV-Raf-1

pNI v2

pCMV-p53

pNG_v2

pCMV-IκBα

pMUTIN4

pCMV-CREB

pMG36e

pCaspase3-sensor

pMetLuc2-Reporter

pCantab 5E

pMetLuc2-Control

pBT2

pmCherry-1

pBR322

pMA09-J

pBIG2R-HPH2

pMA09-H

pAP-1-Luc

pMA5MCS

pAN8-1

pKOR1

pAD43-25

pkc1139

pAD43-25

pK18mobSacB

pAcGFP1-1

pJLA502

HRV 3C protease

pIκB-EGFP

GG499

pAN7-1

p53-Luc

pAP1(PMA)-TA-Luc