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SuperTopFlash载体图谱

SuperTopFlash

SuperTopFlash

SuperTopFlash

 



编号

载体名称

北京华越洋VECT75343

SuperTopFlash

 

SuperTopFlash载体基本信息:

载体名称:

SuperTopFlash

质粒类型:

信号通路报告系统载体

高拷贝/低拷贝:

高拷贝

启动子:

--

克隆方法:

多克隆位点,限制性内切酶

载体大小:

4997bp

5'   测序引物及序列:

RVPrimer3:   CTAGCAAAATAGGCTGTCCC

3'   测序引物及序列:

LucNRev:   CCTTATGCAGTTGCTCTCC

载体标签:

--

载体抗性:

Ampicillin

筛选标记:

--

备注:

克隆菌株DH5alpha

稳定性:

--

组成型:

--

病毒/非病毒:

--

 

 

SuperTopFlash载体质粒图谱和多克隆位点信息

SuperTopFlash载体序列:

ORIGIN
    1 ggtaccgagc tcttacgcga gatcaaaggg ggtaagatca aagggggtaa gatcaaaggg
   61 ggtaagatca aaggcgcgag atcaaagggg gtaagatcaa agggggtaag atcaaagggg
  121 gtaagatcaa aggggcgcgc ccgcgtgcta gcccgggctc gagatctaga ctctagaggg
  181 tatataatgg aagctcgaat tccagcttgg cattccggta ctgttggtaa aaagcttggc
  241 attccggtac tgttggtaaa gccaccatgg aagacgccaa aaacataaag aaaggcccgg
  301 cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag gctatgaaga
  361 gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag gtggacatca
  421 cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc
  481 tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg
  541 tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac
  601 gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg
  661 ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa attattatca
  721 tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc
  781 tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg gacaagacaa
  841 ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc
  901 atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca
  961 ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta
 1021 cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc
 1081 tgtttctgag gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat
 1141 tctccttctt cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa
 1201 ttgcttctgg tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc
 1261 atctgccagg tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta
 1321 cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga
 1381 aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg
 1441 tgagaggtcc tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga
 1501 ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact
 1561 tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg
 1621 ctgaattgga atccatcttg ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc
 1681 ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga
 1741 cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt
 1801 tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg
 1861 caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtgtaat
 1921 tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
 1981 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
 2041 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
 2101 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
 2161 acaaatgtgg taaaatcgat aaggatccgt cgaccgatgc ccttgagagc cttcaaccca
 2221 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
 2281 tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga
 2341 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
 2401 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
 2461 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
 2521 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
 2581 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
 2641 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
 2701 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
 2761 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
 2821 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
 2881 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
 2941 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
 3001 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
 3061 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
 3121 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
 3181 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
 3241 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
 3301 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
 3361 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
 3421 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
 3481 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
 3541 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
 3601 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
 3661 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
 3721 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
 3781 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
 3841 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
 3901 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
 3961 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
 4021 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
 4081 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
 4141 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
 4201 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg
 4261 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
 4321 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
 4381 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
 4441 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
 4501 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
 4561 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
 4621 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
 4681 taacaaaata ttaacgttta caatttccca ttcgccattc aggctgcgca actgttggga
 4741 agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat gataagtaag
 4801 taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt attttcatta
 4861 catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac
 4921 aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga
 4981 acatttctct atcgata
//


 

 

SuperTopFlash载体其他相关的其他类型载体:

TOPFlash

pISRE-TA-Luc

TEV protease

pIL253

SuperTopFlash

pIJ8860

pZsGreen1-DR

pHY300PLK

pZsGreen1-1

pHY300

pZL507

pHT304

pYCT

pHT43

pXMJ19

pHT01

pVLT33

pHSE-Luc

pUG6

pHIS1525

pTimer-1

pHGF9050

pTimer

pHD_v2

pTA-Luc

pHcRed1-DR

pTALETF_v2 (NN)

pHcRed1-1

pTALETF_v2 (NI)

pHCMC05

pTALETF_v2 (NG)

pHCMC04

pTALETF_v2 (HD)

pGRE-luc

pTALEN_v2 (NN)

pGFP315

pTALEN_v2 (NI)

pGFP22

pTALEN_v2 (NG)

pGEM-3

pTALEN_v2 (HD)

pGAS-TA-Luc

pSRE-Luc

pE2F-TA-Luc

pSH65

pDsRed-Express-DR

pSH63

pDsRed-Express2-1

pSH47

pDsRed-Express-1

pSG1154

pDsRed2-1

pset152

pDG1363

pRK2013

pCSN44

pQE-TriSystem

pDD-tdTomato Reporter

pOS7001

pCRE-Luc

pNZ8048

pCR-Blunt

pNN_v2

pCMV-Raf-1

pNI v2

pCMV-p53

pNG_v2

pCMV-IκBα

pMUTIN4

pCMV-CREB

pMG36e

pCaspase3-sensor

pMetLuc2-Reporter

pCantab 5E

pMetLuc2-Control

pBT2

pmCherry-1

pBR322

pMA09-J

pBIG2R-HPH2

pMA09-H

pAP-1-Luc

pMA5MCS

pAN8-1

pKOR1

pAD43-25

pkc1139

pAD43-25

pK18mobSacB

pAcGFP1-1

pJLA502

HRV 3C protease

pIκB-EGFP

GG499

pAN7-1

p53-Luc