pNTAP-Shuttle-C
pNTAP-Shuttle-C
编号 | 载体名称 |
北京华越洋VECT10018 | pNTAP-Shuttle-C |
pNTAP-Shuttle-C载体基本信息
载体名称: | pNTAP-Shuttle-C |
质粒类型: | 腺病毒载体 |
高拷贝/低拷贝: | 低拷贝 |
克隆方法: | 限制性内切酶，多克隆位点 |
启动子: | CMV |
载体大小: | 7737 bp |
5' 测序引物及序列: | CMV Forward： CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | -- |
载体标签: | SBP; CBP (N-端） |
载体抗性: | 卡那霉素 |
筛选标记: | 嘌呤霉素（Puromycin） |
克隆菌株: | DH5α |
宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
备注: | 腺病毒载体pNTAP-Shuttle-C载体表达表达N端融合链霉亲和素肽和钙结合肽的目的蛋白。 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 腺病毒 |
pNTAP-Shuttle-C载体质粒图谱和多克隆位点信息:
pNTAP-Shuttle-C载体序列:
PNTAP SHUTTLE C, 7737 BP VERSION 016001
NOTE: THE FOLLOWING SEQUENCE HAS BEEN VERIFIED FOR ACCURACY
AT THE JUNCTIONS. THE REMAINDER OF THE SEQUENCE HAS BEEN
OBTAINED FROM EXISTING DATA.
1 CATCATCAAT AATATACCTT ATTTTGGATT GAAGCCAATA TGATAATGAG
51 GGGGTGGAGT TTGTGACGTG GCGCGGGGCG TGGGAACGGG GCGGGTGACG
101 TAGTAGTGTG GCGGAAGTGT GATGTTGCAA GTGTGGCGGA ACACATGTAA
151 GCGACGGATG TGGCAAAAGT GACGTTTTTG GTGTGCGCCG GTGTACACAG
201 GAAGTGACAA TTTTCGCGCG GTTTTAGGCG GATGTTGTAG TAAATTTGGG
251 CGTAACCGAG TAAGATTTGG CCATTTTCGC GGGAAAACTG AATAAGAGGA
301 AGTGAAATCT GAATAATTTT GTGTTACTCA TAGCGCGTAA TACTGTAATA
351 GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG
401 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC
451 CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG
501 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT
551 TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC
601 AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG
651 GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA
701 TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA GCGGTTTGAC
751 TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT
801 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC
851 CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
901 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGAGATCTG CTAGCATGAA
951 GCGACGATGG AAAAAGAATT TCATAGCCGT CTCAGCAGCC AACCGCTTTA
1001 AGAAAATCTC ATCCTCCGGG GCACTTGGAA GCGGTAGCGG TACCATGGAC
1051 GAGAAGACCA CCGGCTGGCG GGGCGGCCAC GTGGTGGAGG GCCTGGCCGG
1101 CGAGCTGGAG CAGCTGCGGG CCAGGCTGGA GCACCACCCT CAGGGCCAGC
1151 GGGAGCCCTC CGGCGGCTGC AAGCTGGGCT TGCCCGGGCG GATCCCCCGG
1201 GCGTCGACGC GGCCGCTCGA GCCGAAGCTT ATCGATACGC GTCCGATCGG
1251 ATATCCGATC CACCGGATCT AGATAACTGA TCATAATCAG CCATACCACA
1301 TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA
1351 CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG
1401 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA
1451 GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT
1501 ATCTTAACGC GGATCTGGGC GTGGTTAAGG GTGGGAAAGA ATATATAAGG
1551 TGGGGGTCTT ATGTAGTTTT GTATCTGTTT TGCAGCAGCC GCCGCCGCCA
1601 TGAGCACCAA CTCGTTTGAT GGAAGCATTG TGAGCTCATA TTTGACAACG
1651 CGCATGCCCC CATGGGCCGG GGTGCGTCAG AATGTGATGG GCTCCAGCAT
1701 TGATGGTCGC CCCGTCCTGC CCGCAAACTC TACTACCTTG ACCTACGAGA
1751 CCGTGTCTGG AACGCCGTTG GAGACTGCAG CCTCCGCCGC CGCTTCAGCC
1801 GCTGCAGCCA CCGCCCGCGG GATTGTGACT GACTTTGCTT TCCTGAGCCC
1851 GCTTGCAAGC AGTGCAGCTT CCCGTTCATC CGCCCGCGAT GACAAGTTGA
1901 CGGCTCTTTT GGCACAATTG GATTCTTTGA CCCGGGAACT TAATGTCGTT
1951 TCTCAGCAGC TGTTGGATCT GCGCCAGCAG GTTTCTGCCC TGAAGGCTTC
2001 CTCCCCTCCC AATGCGGTTT AAAACATAAA TAAAAAACCA GACTCTGTTT
2051 GGATTTGGAT CAAGCAAGTG TCTTGCTGTC TTTATTTAGG GGTTTTGCGC
2101 GCGCGGTAGG CCCGGGACCA GCGGTCTCGG TCGTTGAGGG TCCTGTGTAT
2151 TTTTTCCAGG ACGTGGTAAA GGTGACTCTG GATGTTCAGA TACATGGGCA
2201 TAAGCCCGTC TCTGGGGTGG AGGTAGCACC ACTGCAGAGC TTCATGCTGC
2251 GGGGTGGTGT TGTAGATGAT CCAGTCGTAG CAGGAGCGCT GGGCGTGGTG
2301 CCTAAAAATG TCTTTCAGTA GCAAGCTGAT TGCCAGGGGC AGGCCCTTGG
2351 TGTAAGTGTT TACAAAGCGG TTAAGCTGGG ATGGGTGCAT ACGTGGGGAT
2401 ATGAGATGCA TCTTGGACTG TATTTTTAGG TTGGCTATGT TCCCAGCCAT
2451 ATCCCTCCGG GGATTCATGT TGTGCAGAAC CACCAGCACA GTGTATCCGG
2501 TGCACTTGGG AAATTTGTCA TGTAGCTTAG AAGGAAATGC GTGGAAGAAC
2551 TTGGAGACGC CCTTGTGACC TCCAAGATTT TCCATGCATT CGTCCATAAT
2601 GATGGCAATG GGCCCACGGG CGGCGGCCTG GGCGAAGATA TTTCTGGGAT
2651 CACTAACGTC ATAGTTGTGT TCCAGGATGA GATCGTCATA GGCCATTTTT
2701 ACAAAGCGCG GGCGGAGGGT GCCAGACTGC GGTATAATGG TTCCATCCGG
2751 CCCAGGGGCG TAGTTACCCT CACAGATTTG CATTTCCCAC GCTTTGAGTT
2801 CAGATGGGGG GATCATGTCT ACCTGCGGGG CGATGAAGAA AACGGTTTCC
2851 GGGGTAGGGG AGATCAGCTG GGAAGAAAGC AGGTTCCTGA GCAGCTGCGA
2901 CTTACCGCAG CCGGTGGGCC CGTAAATCAC ACCTATTACC GGGTGCAACT
2951 GGTAGTTAAG AGAGCTGCAG CTGCCGTCAT CCCTGAGCAG GGGGGCCACT
3001 TCGTTAAGCA TGTCCCTGAC TCGCATGTTT TCCCTGACCA AATCCGCCAG
3051 AAGGCGCTCG CCGCCCAGCG ATAGCAGTTC TTGCAAGGAA GCAAAGTTTT
3101 TCAACGGTTT GAGACCGTCC GCCGTAGGCA TGCTTTTGAG CGTTTGACCA
3151 AGCAGTTCCA GGCGGTCCCA CAGCTCGGTC ACCTGCTCTA CGGCATCTCG
3201 ATCCAGCATA TCTCCTCGTT TCGCGGGTTG GGGCGGCTTT CGCTGTACGG
3251 CAGTAGTCGG TGCTCGTCCA GACGGGCCAG GGTCATGTCT TTCCACGGGC
3301 GCAGGGTCCT CGTCAGCGTA GTCTGGGTCA CGGTGAAGGG GTGCGCTCCG
3351 GGCTGCGCGC TGGCCAGGGT GCGCTTGAGG CTGGTCCTGC TGGTGCTGAA
3401 GCGCTGCCGG TCTTCGCCCT GCGCGTCGGC CAGGTAGCAT TTGACCATGG
3451 TGTCATAGTC CAGCCCCTCC GCGGCGTGGC CCTTGGCGCG CAGCTTGCCC
3501 TTGGAGGAGG CGCCGCACGA GGGGCAGTGC AGACTTTTGA GGGCGTAGAG
3551 CTTGGGCGCG AGAAATACCG ATTCCGGGGA GTAGGCATCC GCGCCGCAGG
3601 CCCCGCAGAC GGTCTCGCAT TCCACGAGCC AGGTGAGCTC TGGCCGTTCG
3651 GGGTCAAAAA CCAGGTTTCC CCCATGCTTT TTGATGCGTT TCTTACCTCT
3701 GGTTTCCATG AGCCGGTGTC CACGCTCGGT GACGAAAAGG CTGTCCGTGT
3751 CCCCGTATAC AGACTTGAGA GGGAGTTTAA ACGAATTCAA TAGCTTGTTG
3801 CATGGGCGGC GATATAAAAT GCAAGGTGCT GCTCAAAAAA TCAGGCAAAG
3851 CCTCGCGCAA AAAAGAAAGC ACATCGTAGT CATGCTCATG CAGATAAAGG
3901 CAGGTAAGCT CCGGAACCAC CACAGAAAAA GACACCATTT TTCTCTCAAA
3951 CATGTCTGCG GGTTTCTGCA TAAACACAAA ATAAAATAAC AAAAAAACAT
4001 TTAAACATTA GAAGCCTGTC TTACAACAGG AAAAACAACC CTTATAAGCA
4051 TAAGACGGAC TACGGCCATG CCGGCGTGAC CGTAAAAAAA CTGGTCACCG
4101 TGATTAAAAA GCACCACCGA CAGCTCCTCG GTCATGTCCG GAGTCATAAT
4151 GTAAGACTCG GTAAACACAT CAGGTTGATT CACATCGGTC AGTGCTAAAA
4201 AGCGACCGAA ATAGCCCGGG GGAATACATA CCCGCAGGCG TAGAGACAAC
4251 ATTACAGCCC CCATAGGAGG TATAACAAAA TTAATAGGAG AGAAAAACAC
4301 ATAAACACCT GAAAAACCCT CCTGCCTAGG CAAAATAGCA CCCTCCCGCT
4351 CCAGAACAAC ATACAGCGCT TCCACAGCGG CAGCCATAAC AGTCAGCCTT
4401 ACCAGTAAAA AAGAAAACCT ATTAAAAAAA CACCACTCGA CACGGCACCA
4451 GCTCAATCAG TCACAGTGTA AAAAAGGGCC AAGTGCAGAG CGAGTATATA
4501 TAGGACTAAA AAATGACGTA ACGGTTAAAG TCCACAAAAA ACACCCAGAA
4551 AACCGCACGC GAACCTACGC CCAGAAACGA AAGCCAAAAA ACCCACAACT
4601 TCCTCAAATC GTCACTTCCG TTTTCCCACG TTACGTCACT TCCCATTTTA
4651 AGAAAACTAC AATTCCCAAC ACATACAAGT TACTCCGCCC TAAAACCTAC
4701 GTCACCCGCC CCGTTCCCAC GCCCCGCGCC ACGTCACAAA CTCCACCCCC
4751 TCATTATCAT ATTGGCTTCA ATCCAAAATA AGGTATATTA TTGATGATGT
4801 TAATTAACAT GCATGGATCC ATATGCGGTG TGAAATACCG CACAGATGCG
4851 TAAGGAGAAA ATACCGCATC AGGCGCTCTT CCGCTTCCTC GCTCACTGAC
4901 TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA
4951 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA
5001 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG
5051 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
5101 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG
5151 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG
5201 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
5251 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA
5301 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA
5351 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
5401 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG
5451 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG
5501 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
5551 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT
5601 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA
5651 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
5701 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA
5751 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG
5801 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC
5851 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT
5901 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
5951 ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA
6001 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT
6051 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA
6101 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTGCAGCCA TGAGATTATC
6151 AAAAAGGATC TTCACCTAGA TCCTTTTCAC GTAGAAAGCC AGTCCGCAGA
6201 AACGGTGCTG ACCCCGGATG AATGTCAGCT ACTGGGCTAT CTGGACAAGG
6251 GAAAACGCAA GCGCAAAGAG AAAGCAGGTA GCTTGCAGTG GGCTTACATG
6301 GCGATAGCTA GACTGGGCGG TTTTATGGAC AGCAAGCGAA CCGGAATTGC
6351 CAGCTGGGGC GCCCTCTGGT AAGGTTGGGA AGCCCTGCAA AGTAAACTGG
6401 ATGGCTTTCT TGCCGCCAAG GATCTGATGG CGCAGGGGAT CAAGCTCTGA
6451 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC
6501 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG
6551 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC
6601 GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
6651 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC
6701 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG
6751 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG
6801 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT
6851 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT
6901 CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
6951 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC
7001 AAGGCGAGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC
7051 CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG
7101 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT
7151 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT
7201 CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
7251 GCCTTCTTGA CGAGTTCTTC TGAATTTTGT TAAAATTTTT GTTAAATCAG
7301 CTCATTTTTT AACCAATAGG CCGAAATCGG CACCATCCCT TATAAATCAA
7351 AAGAATAGAC CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT
7401 CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA AAACCGTCTA
7451 TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA AGTTTTTTGT
7501 GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG GAGCCCCCGA
7551 TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA
7601 GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC
7651 TGCGCGTAAC CACCACACCC GCGCGCTTAA TGCGCCGCTA CAGGGCGCGT
7701 CCATTCGCCA TTCAGGATCG AATTAATTCT TAATTAA
pNTAP-Shuttle-C其他腺病毒表达载体：
