pAAV-hSyn-RFP
pAAV-hSyn-RFP
编号 | 载体名称 |
北京华越洋VECT10040 | pAAV-hSyn-RFP |
pAAV-hSyn-RFP载体基本信息：
载体名称: | pAAV-hSyn-RFP |
质粒类型: | 腺病毒载体；荧光报告载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶，多克隆位点 |
启动子: | human Synapsin 1 |
载体大小: | 5909 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | SP6 5'ATTTAGGTGACACTATAG 3' |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | 红色荧光蛋白DsRedExpress |
克隆菌株: | Stbl3 或 NEB Stble |
宿主细胞（系）: | 神经细胞 |
备注: | pAAV-hSyn-RFP载体含有human synapsin 1 启动子，适合在神经细胞中表达。 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 腺病毒 |
pAAV-hSyn-RFP载体质粒图谱和多克隆位点信息：
pAAV-hSyn-RFP载体序列：
ORIGIN
1 GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC
61 GAGCGCAGCG AGTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
121 AGGGGTTCCT TGTAGTTAAT GATTAACCCG CCATGCTACT TATCTACGTA GCCATGCTCT
181 AGAGGATCCT TCGAAAAGCT TCTGCAGAGG GCCCTGCGTA TGAGTGCAAG TGGGTTTTAG
241 GACCAGGATG AGGCGGGGTG GGGGTGCCTA CCTGACGACC GACCCCGACC CACTGGACAA
301 GCACCCAACC CCCATTCCCC AAATTGCGCA TCCCCTATCA GAGAGGGGGA GGGGAAACAG
361 GATGCGGCGA GGCGCGTGCG CACTGCCAGC TTCAGCACCG CGGACAGTGC CTTCGCCCCC
421 GCCTGGCGGC GCGCGCCACC GCCGCCTCAG CACTGAAGGC GCGCTGACGT CACTCGCCGG
481 TCCCCCGCAA ACTCCCCTTC CCGGCCACCT TGGTCGCGTC CGCGCCGCCG CCGGCCCAGC
541 CGGACCGCAC CACGCGAGGC GCGAGATAGG GGGGCACGGG CGCGACCATC TGCGCTGCGG
601 CGCCGGCGAC TCAGCGCTGC CTCAGTCTGC GGTGGGCAGC GGAGGAGTCG TGTCGTGCCT
661 GAGAGCGCAG TCGAGGCGCG CCGAGCTCGG ATCCTGAGAA CTTCAGGGTG AGTCTATGGG
721 ACCCTTGATG TTTTCTTTCC CCTTCTTTTC TATGGTTAAG TTCATGTCAT AGGAAGGGGA
781 GAAGTAACAG GGTACACATA TTGACCAAAT CAGGGTAATT TTGCATTTGT AATTTTAAAA
841 AATGCTTTCT TCTTTTAATA TACTTTTTTG TTTATCTTAT TTCTAATACT TTCCCTAATC
901 TCTTTCTTTC AGGGCAATAA TGATACAATG TATCATGCCT CTTTGCACCA TTCTAAAGAA
961 TAACAGTGAT AATTTCTGGG TTAAGGCAAT AGCAATATTT CTGCATATAA ATATTTCTGC
1021 ATATAAATTG TAACTGATGT AAGAGGTTTC ATATTGCTAA TAGCAGCTAC AATCCAGCTA
1081 CCATTCTGCT TTTATTTTAT GGTTGGGATA AGGCTGGATT ATTCTGAGTC CAAGCTAGGC
1141 CCTTTTGCTA ATCATGTTCA TACCTCTTAT CTTCCTCCCA CAGCTCCTGG GCAACGTGCT
1201 GGTCTGTGTG CTGGCCCATC ACTTTGGCAA AGCACGTGAA TTCGAAGATC TGGCCGCCTC
1261 GGCCTCTAGA ACTAGTGGAT CCCCCGGGCT GCAGGAATTC GATGCTACCG GTCGCCACCA
1321 TGGCCTCCTC CGAGGACGTC ATCAAGGAGT TCATGCGCTT CAAGGTGCGC ATGGAGGGCT
1381 CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC TACGAGGGCA
1441 CCCAGACCGC CAAGCTGAAG GTGACCAAGG GCGGCCCCCT GCCCTTCGCC TGGGACATCC
1501 TGTCCCCCCA GTTCCAGTAC GGCTCCAAGG TGTACGTGAA GCACCCCGCC GACATCCCCG
1561 ACTACAAGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG AACTTCGAGG
1621 ACGGCGGCGT GGTGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCTCC TTCATCTACA
1681 AGGTGAAGTT CATCGGCGTG AACTTCCCCT CCGACGGCCC CGTAATGCAG AAGAAGACTA
1741 TGGGCTGGGA GGCCTCCACC GAGCGCCTGT ACCCCCGCGA CGGCGTGCTG AAGGGCGAGA
1801 TCCACAAGGC CCTGAAGCTG AAGGACGGCG GCCACTACCT GGTGGAGTTC AAGTCCATCT
1861 ACATGGCCAA GAAGCCCGTG CAGCTGCCCG GCTACTACTA CGTGGACTCC AAGCTGGACA
1921 TCACCTCCCA CAACGAGGAC TACACCATCG TGGAGCAGTA CGAGCGCGCC GAGGGCCGCC
1981 ACCACCTGTT CCTGTAGCGG CCAAATGAAT TCCCAAACCG TACGGGAAAC GGCCCTATTC
2041 TATAGTGTCA CCTAAATGCT AGAGCTCGCT GATCAGCCTC GACTGTGCCT TCTAGTTGCC
2101 AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA
2161 CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA
2221 TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCGCGG
2281 CCGCTCTAGA GCATGGCTAC GTAGATAAGT AGCATGGCGG GTTAATCATT AACTACAAGG
2341 AACCCCTAGT GATGGAGTTG GCCACTCCCT CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG
2401 GGCGACCAAA GGTCGCCCGA CGCCCGGGCT TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG
2461 CGCGCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA
2521 GCCTGAATGG CGAATGGCGA TTCCGTTGCA ATGGCTGGCG GTAATATTGT TCTGGATATT
2581 ACCAGCAAGG CCGATAGTTT GAGTTCTTCT ACTCAGGCAA GTGATGTTAT TACTAATCAA
2641 AGAAGTATTG CGACAACGGT TAATTTGCGT GATGGACAGA CTCTTTTACT CGGTGGCCTC
2701 ACTGATTATA AAAACACTTC TCAGGATTCT GGCGTACCGT TCCTGTCTAA AATCCCTTTA
2761 ATCGGCCTCC TGTTTAGCTC CCGCTCTGAT TCTAACGAGG AAAGCACGTT ATACGTGCTC
2821 GTCAAAGCAA CCATAGTACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT
2881 TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT
2941 CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC
3001 TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA
3061 TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC
3121 CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT
3181 CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT
3241 GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTTAAATATT
3301 TGCTTATACA ATCTTCCTGT TTTTGGGGCT TTTCTGATTA TCAACCGGGG TACATATGAT
3361 TGACATGCTA GTTTTACGAT TACCGTTCAT CGATTCTCTT GTTTGCTCCA GACTCTCAGG
3421 CAATGACCTG ATAGCCTTTG TAGAGACCTC TCAAAAATAG CTACCCTCTC CGGCATGAAT
3481 TTATCAGCTA GAACGGTTGA ATATCATATT GATGGTGATT TGACTGTCTC CGGCCTTTCT
3541 CACCCGTTTG AATCTTTACC TACACATTAC TCAGGCATTG CATTTAAAAT ATATGAGGGT
3601 TCTAAAAATT TTTATCCTTG CGTTGAAATA AAGGCTTCTC CCGCAAAAGT ATTACAGGGT
3661 CATAATGTTT TTGGTACAAC CGATTTAGCT TTATGCTCTG AGGCTTTATT GCTTAATTTT
3721 GCTAATTCTT TGCCTTGCCT GTATGATTTA TTGGATGTTG GAATCGCCTG ATGCGGTATT
3781 TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC AGTACAATCT
3841 GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT
3901 GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC Tccgggagct
3961 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagACGAAAG GGCCTCGTGA
4021 TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA
4081 CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA
4141 TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA
4201 GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC
4261 CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG
4321 CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC
4381 CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT
4441 CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT
4501 TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT
4561 TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA
4621 TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC
4681 TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA
4741 TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG
4801 CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC
4861 GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT
4921 CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT
4981 ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG
5041 CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG
5101 ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA
5161 TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA
5221 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA
5281 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA
5341 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT
5401 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT
5461 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT
5521 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT
5581 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA
5641 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG
5701 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC
5761 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA
5821 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA
5881 TGTTCTTTCC TGCGTTATCC CCTGATTCT
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pAAV-hSyn-RFP其他腺病毒表达载体：
