pShuttle-IRES-hrGFP-2
pShuttle-IRES-hrGFP-2
编号 | 载体名称 |
北京华越洋VECT10006 | pShuttle-IRES-hrGFP-2 |
pShuttle-IRES-hrGFP-2载体基本信息:
载体名称: | pShuttle-IRES-hrGFP-2 |
质粒类型: | 腺病毒载体；双顺反子载体；荧光报告载体 |
高拷贝/低拷贝: | 低拷贝 |
启动子: | CMV |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 8.9 kb |
5' 测序引物及序列: | pShuttle-IRES-hrGFP-1-F: CTCACGGGGATTTCCAAGTC |
3' 测序引物及序列: | pShuttle-IRES-hrGFP-1-R: ATGCAGTCGTCGAGGAATTG |
载体标签: | -- |
载体抗性: | 卡那霉素 |
备注: | 载体内包含有IRES序列和hrGFP序列。 |
稳定性: | 稳表达 |
组成型: | 组成型 |
病毒/非病毒: | 腺病毒 |
载体名称: | pShuttle-IRES-hrGFP-2 |
质粒类型: | 腺病毒载体；双顺反子载体；荧光报告载体 |
pShuttle-IRES-hrGFP-2载体质粒图谱和多克隆位点信息:
pShuttle-IRES-hrGFP-2载体简介:
Two additional shuttle vectors, pShuttle-IRES-hrGFP-1 (Catalog #240081) and pShuttle-IRES-hrGFP-2 (Catalog #240082) are available separately. Both of these vectors contain the CMV promoter and a dicistronic expression cassette in which the multiple cloning site (MCS) is followed by the EMCV-IRES, which directs translation of a humanized recombinant green fluorescent protein (hrGFP) from a novel marine organism as a second open reading frame. This design allows the expression of the gene of interest (up to 5.2 kb) to be monitored at the single-cell level due to expression of the hrGFP on the same transcript. The gene of interest may be fused to three contiguous copies of either the FLAG® epitope (pShuttle-IRES-hrGFP-1) or the HA epitope (pShuttle-IRES-hrGFP-2).
pShuttle-IRES-hrGFP-2载体序列
pShuttle-IRES-hrGFP-2其他腺病毒表达载体：
