北京华越洋生物
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148

pDC312

pDC312

pDC312

 

编号

载体名称

北京华越洋VECT10034

pDC312

 

pDC312载体基本信息

载体名称:

pDC312

质粒类型:

腺病毒表达载体

高拷贝/低拷贝:

--

启动子:

--

克隆方法:

--

载体大小:

3287 bp

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

--

载体抗性:

氨苄青霉素(Ampicillin

筛选标记:

--

备注:

克隆宿主菌:DB3.1

稳定性:

--

组成型:

组成型

病毒/非病毒:

腺病毒

 

pDC312载体质粒图谱和多克隆位点信息

 

pDC312载体序列:

ORIGIN

        1 TTCATCAATA ATATACCTTA TTTTGGATTG AAGCCAATAT GATAATGAGG GGGTGGAGTT

       61 TGTGACGTGG CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG

      121 ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG ACGTTTTTGG

      181 TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT

      241 AAATTTGGGC GTAACCGAGT AAGATTTGGC CATTTTCGCG GGAAAACTGA ATAAGAGGAA

      301 GTGAAATCTG AATAATTTTG TGTTACTCAT AGCGCGTAAT ATTTGTCTAG GGCCGCGGGG

      361 ACTTTGACCG TTTACGTGGA GACTCGCCCA GGTGTTTTTC TCAGGTGTTT TCCGCGTTCC

      421 GGGTCAAAGT TGGCGTTTTA TTATTATAGT CAGNTCTAGA GTCGACGAGC TCAAGCTTAG

      481 ATCTGCTAGC AGGCCTGAAT TCGGATCCTC GACTCGAAGA TCCAATAACT TCGTATAGCA

      541 TACATTATAC GAAGTTATAA GTGCCGCCCT ATACCTTGTC TGCCTCCCCG CGTTGCGTCG

      601 CGGTGCATGG AGCCGGGCCA CCTCGACCTG AATGGAAGCC GGCGGCACCT CGCTAACGGA

      661 TTCACCACTC CAAGAATTGG AGCCAATCAA TTCTTGCGGA GAACTGTGAA TGCGCAAACC

      721 AACCCTTGGC AGAACATATC CATCGCGTCC GCCATCTCCA GCAGCCGCAC GCGGCGCATC

      781 TCGGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC

      841 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC

      901 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA

      961 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT

     1021 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG

     1081 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG

     1141 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG

     1201 CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC

     1261 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA

     1321 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG

     1381 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC

     1441 TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC

     1501 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG

     1561 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT

     1621 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT

     1681 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA

     1741 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA

     1801 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT

     1861 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG

     1921 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA

     1981 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA

     2041 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG

     2101 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC

     2161 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC

     2221 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA

     2281 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC

     2341 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAACACG

     2401 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC

     2461 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG

     2521 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC

     2581 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT

     2641 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA

     2701 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA

     2761 AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG

     2821 TATCACTCTA GGCAAAATAG CACCCTCCCG CTCCAGAACA ACATACAGCG CTTCACAGCG

     2881 GCAGCCTAAC AGTCAGCCTT ACCAGTAAAA AAGAAAACCT ATTAAAAAAA CACCACTCGA

     2941 CACGGCACCA GCTCAATCAG TCACAGTGTA AAAAAGGGCC AAGTGCAGAG CGAGTATATA

     3001 TAGGACTAAA AAATGACGTA ACGGTTAAAG TCCACAAAAA ACACCCAGAA AACCGCACGC

     3061 GAACCTACGC CCAGAAACGA AAGCCAAAAA ACCCACAACT TCCTCAAATC GTCACTTCCG

     3121 TTTTCCCACG TTACGTAACT TCCCATTTTA AGAAAACTAC AATTCCCAAC ACATACAAGT

     3181 TACTCCGCCC TAAAACCTAC GTCACCCGCC CCGTTCCCAC GCCCCGCGCC ACGTCACAAA

     3241 CTCCACCCCC TCATTATCAT ATTGGCTTCA ATCCAAAATA AGGTATA

//

 

pDC312其他腺病毒表达载体:

pNTAP-Shuttle-B

pAAV-CAG-RFP

pAAV-CaMKIIa-EGFP

pCTAP-Shuttle-A

pAAV-CMV-Rluc

pNTAP-Shuttle-A

pAAV-CAG-GFP

pBHGloxdelE13cre

pCTAP-Shuttle-C

pDC511

pDC515

pDC312

pAdTrack-CMV

pAAV-IRES-hrGFP

pShuttle-IRES-hrGFP-1

pShuttle-CMV-EGFP-C

pacAd5 9.2-100

pacAd5 CMV-GFP

pAAV-Syn-Rluc

pCMV-MCS

pShuttle-IRES-hrGFP-2

pShuttle-CMV

pAAV-Syn-Rluc

pAAV-hSyn-RFP

pAAV-CMV-iRFP

pAAV-UbC

pDC415

pAAV-CAG-Fluc

pAAV-LacZ

pNTAP-Shuttle-B

pDC316

pDC516

pAAV-CA

pDC416

pNTAP-Shuttle-C

pDC311

pDC512

pAAV-hrGFP

pDC411

pAAV-RC

pAdTrack

pacAd5 CMVK-NpA

pAdEasy-1

pDC315

pAAV-MCS

pShuttle

pHelper

pBApo-CMV-neo

pShuttle-CMV-lacZ

pBApo-CMV

pAAV-TRE-Syn-Fluc

pBApo-CMV-Pur

pAAV-minCMV-mCherry