北京华越洋生物
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首页    载体及质粒    pDC416
9

pDC416

pDC416

pDC416

 

编号

载体名称

北京华越洋VECT10046

pDC416

 

pDC416载体基本信息:

载体名称:

pDC416

质粒类型:

腺病毒表达载体

高拷贝/低拷贝:

--

启动子:

MCMV

克隆方法:

--

载体大小:

5951 bp

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

--

载体抗性:

氨苄青霉素(Ampicillin

筛选标记:

--

备注:

克隆宿主菌:DB3.1

稳定性:

--

组成型:

组成型

病毒/非病毒:

腺病毒

 

pDC416载体质粒图谱和多克隆位点信息:

pDC416载体序列:

ORIGIN

        1 TTCATCAATA ATATACCTTA TTTTGGATTG AAGCCAATAT GATAATGAGG GGGTGGAGTT

       61 TGTGACGTGG CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG

      121 ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG ACGTTTTTGG

      181 TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT

      241 AAATTTGGGC GTAACCGAGT AAGATTTGGC CATTTTCGCG GGAAAACTGA ATAAGAGGAA

      301 GTGAAATCTG AATAATTTTG TGTTACTCAT AGCGCGTAAT ATTTGTCTAG GGCCGCGGGG

      361 ACTTTGACCG TTTACGTGGA GACTCGCCCA GGTGTTTTTC TCAGGTGTTT TCCGCGTTCC

      421 GGGTCAAAGT TGGCGTTTTA TTATTATAGT CAGNTCTAGA GATATACTGA GTCATTAGGG

      481 ACTTTCCAAT GGGTTTTGCC CAGTACATAA GGTCAATAGG GGTGAATCAA CAGGAAAGTC

      541 CCATTGGAGC CAAGTACACT GAGTCAATAG GGACTTTCCA TTGGGTTTTG CCCAGTACAA

      601 AAGGTCAATA GGGGGTGAGT CAATGGGTTT TTCCCATTAT TGGCACGTAC ATAAGGTCAA

      661 TAGGGGTGAG TCATTGGGTT TTTCCAGCCA TTTAATTAAA ACGCCATGTA CTTTCCCACC

      721 ATTGACGTCA ATGGGCTATT GAAACTAATG CAACGTGACC TTTAAACGGT ACTTTCCCAT

      781 AGCTGATTAA TGGGAAAGTA CCGTTCTCGA GCCAATACAC GTCAATGGGA AGTGAAAGGG

      841 CAGCCAAAAC GTAACACCGC CCCGGTTTTC CCCTGGAAAT TCCATATTGG CACTCATTCT

      901 ATTGGCTGAG CTGCGTTCTA CGTGGGTATA AGAGGCGCGA CCAGCGTCGG TACCGTCGCA

      961 GTCTTCGGTC TGACCACCGT AGAACGCAGA TCGAATTCCC CGGGAGATCT AAGCTTGAGC

     1021 TCGTCGACTT CGAGCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC

     1081 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA

     1141 CTCATCAATG TATCTTATCA TGTCTGGATC GTCTAGCATC GAAGATCGAT CTGGGCGTGG

     1201 TTAAGGGTGG GAAAGAATAT ATAAGGTGGG GGTCTTATGT AGTTTTGTAT CTGTTTTGCA

     1261 GCAGCCGCCG CCGCCATGAG CACCAACTCG TTTGATGGAA GCATTGTGAG CTCATATTTG

     1321 ACAACGCGCA TGCCCCCATG GGCCGGGGTG CGTCAGAATG TGATGGGCTC CAGCATTGAT

     1381 GGTCGCCCCG TCCTGCCCGC AAACTCTACT ACCTTGACCT ACGAGACCGT GTCTGGAACG

     1441 CCGTTGGAGA CTGCAGCCTC CGCCGCCGCT TCAGCCGCTG CAGCCACCGC CCGCGGGATT

     1501 GTGACTGACT TTGCTTTCCT GAGCCCGCTT GCAAGCAGTG CAGCTTCCCG TTCATCCGCC

     1561 CGCGATGACA AGTTGACGGC TCTTTTGGCA CAATTGGATT CTTTGACCCG GGAACTTAAT

     1621 GTCGTTTCTC AGCAGCTGTT GGATCTGCGC CAGCAGGTTT CTGCCCTGAA GGCTTCCTCC

     1681 CCTCCCAATG CGGTTTAAAA CATAAATAAA AAACCAGACT CTGTTTGGAT TTGGATCAAG

     1741 CAAGTGTCTT GCTGTCTTTA TTTAGGGGTT TTGCGCGCGC GGTAGGCCCG GGACCAGCGG

     1801 TCTCGGTCGT TGAGGGTCCT GTGTATTTTT TCCAGGACGT GGTAAAGGTG ACTCTGGATG

     1861 TTCAGATACA TGGGCATAAG CCCGTCTCTG GGGTGGAGGT AGCACCACTG CAGAGCTTCA

     1921 TGCTGCGGGG TGGTGTTGTA GATGATCCAG TCGTAGCAGG AGCGCTGGGC GTGGTGCCTA

     1981 AAAATGTCTT TCAGTAGCAA GCTGATTGCC AGGGGCAGGC CCTTGGTGTA AGTGTTTACA

     2041 AAGCGGTTAA GCTGGGATGG GTGCATACGT GGGGATATGA GATGCATCTT GGACTGTATT

     2101 TTTAGGTTGG CTATGTTCCC AGCCATATCC CTCCGGGGAT TCATGTTGTG CAGAACCACC

     2161 AGCACAGTGT ATCCGGTGCA CTTGGGAAAT TTGTCATGTA GCTTAGAAGG AAATGCGTGG

     2221 AAGAACTTGG AGACGCCCTT GTGACCTCCA AGATTTTCCA TGCATTCGTC CATAATGATG

     2281 GCAATGGGCC CACGGGCGGC GGCCTGGGCG AAGATATTTC TGGGATCACT AACGTCATAG

     2341 TTGTGTTCCA GGATGAGATC GTCATAGGCC ATTTTTACAA AGCGCGGGCG GAGGGTGCCA

     2401 GACTGCGGTA TAATGGTTCC ATCCGGCCCA GGGGCGTAGT TACCCTCACA GATTTGCATT

     2461 TCCCACGCTT TGAGTTCAGA TGGGGGGATC ATGTCTACCT GCGGGGCGAT GAAGAAAACG

     2521 GTTTCCGGGG TAGGGGAGAT CAGCTGGGAA GAAAGCAGGT TCCTGAGCAG CTGCGACTTA

     2581 CCGCAGCCGG TGGGCCCGTA AATCACACCT ATTACCGGGT GCAACTGGTA GTTAAGAGAG

     2641 CTGCAGCTGC CGTCATCCCT GAGCAGGGGG GCCACTTCGT TAAGCATGTC CCTGACTCGC

     2701 ATGTTTTCCC TGACCAAATC CGCCAGAAGG CGCTCGCCGC CCAGCGATAG CAGTTCTTGC

     2761 AAGGAAGCAA AGTTTTTCAA CGGTTTGAGA CCGTCCGCCG TAGGCATGCT TTTGAGCGTT

     2821 TGACCAAGCA GTTCCAGGCG GTCCCACAGC TCGGTCACCT GCTCTACGGC ATCTCGATCC

     2881 AGCATATCTC CTCGTTTCGC GGGTTGGGGC GGCTTTCGCT GTACGGCAGT AGTCGGTGCT

     2941 CGTCCAGACG GGCCAGGGTC ATGTCTTTCC ACGGGCGCAG GGTCCTCGTC AGCGTAGTCT

     3001 GGGTCACGGT GAAGGGGTGC GCTCCGGGCT GCGCGCTGGC CAGGGTGCGC TTGAGGCTGG

     3061 TCCTGCTGGT GCTGAAGCGC TGCCGGTCTT CGCCCTGCGC GTCGGCCAGG TAGCATTTGA

     3121 CCATGGTGTC ATAGTCCAGC CCCTCCGCGG CGTGGCCCTT GGCGCGCAGC TTGCCCTTGG

     3181 AGGAGGCGCC GCACGAGGGG CAGTGCAGAC TTTTGAGGGC GTAGAGCTTG GGCGCGAGAA

     3241 ATACCGATTC CGGGGAGTAG GCATCCGCGC CGCAGGCCCC GCAGACGGTC TCGCATTCCA

     3301 CGAGCCAGGT GAGCTCTGGC CGTTCGGGGT CAAAAACCAG GTTTCCCCCA TGCTTTTTGA

     3361 TGCGTTTCTT ACCTCTGGTT TCCATGAGCC GGTGTCCACG CTCGGTGACG AAAAGGCTGT

     3421 CCGTGTCCCC GTATACAGAC TTGAGAGGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG

     3481 AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT

     3541 CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA

     3601 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG

     3661 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA

     3721 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT

     3781 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT

     3841 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT

     3901 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC

     3961 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT

     4021 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC

     4081 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT

     4141 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA

     4201 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA

     4261 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA

     4321 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT

     4381 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA

     4441 CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC

     4501 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG

     4561 CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT

     4621 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT

     4681 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG

     4741 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC

     4801 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA

     4861 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC

     4921 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT

     4981 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG

     5041 TTGCTCTTGC CCGGCGTCAA CACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT

     5101 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG

     5161 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC

     5221 CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC

     5281 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA

     5341 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG

     5401 GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT

     5461 GACATTAACC TATAAAAATA GGCGTATCAC TCTAGGCAAA ATAGCACCCT CCCGCTCCAG

     5521 AACAACATAC AGCGCTTCAC AGCGGCAGCC TAACAGTCAG CCTTACCAGT AAAAAAGAAA

     5581 ACCTATTAAA AAAACACCAC TCGACACGGC ACCAGCTCAA TCAGTCACAG TGTAAAAAAG

     5641 GGCCAAGTGC AGAGCGAGTA TATATAGGAC TAAAAAATGA CGTAACGGTT AAAGTCCACA

     5701 AAAAACACCC AGAAAACCGC ACGCGAACCT ACGCCCAGAA ACGAAAGCCA AAAAACCCAC

     5761 AACTTCCTCA AATCGTCACT TCCGTTTTCC CACGTTACGT AACTTCCCAT TTTAAGAAAA

     5821 CTACAATTCC CAACACATAC AAGTTACTCC GCCCTAAAAC CTACGTCACC CGCCCCGTTC

     5881 CCACGCCCCG CGCCACGTCA CAAACTCCAC CCCCTCATTA TCATATTGGC TTCAATCCAA

     5941 AATAAGGTAT A

//

 

pDC416其他腺病毒表达载体:

pNTAP-Shuttle-B

pAAV-CAG-RFP

pAAV-CaMKIIa-EGFP

pCTAP-Shuttle-A

pAAV-CMV-Rluc

pNTAP-Shuttle-A

pAAV-CAG-GFP

pBHGloxdelE13cre

pCTAP-Shuttle-C

pDC511

pDC515

pDC312

pAdTrack-CMV

pAAV-IRES-hrGFP

pShuttle-IRES-hrGFP-1

pShuttle-CMV-EGFP-C

pacAd5 9.2-100

pacAd5 CMV-GFP

pAAV-Syn-Rluc

pCMV-MCS

pShuttle-IRES-hrGFP-2

pShuttle-CMV

pAAV-Syn-Rluc

pAAV-hSyn-RFP

pAAV-CMV-iRFP

pAAV-UbC

pDC415

pAAV-CAG-Fluc

pAAV-LacZ

pNTAP-Shuttle-B

pDC316

pDC516

pAAV-CA

pDC416

pNTAP-Shuttle-C

pDC311

pDC512

pAAV-hrGFP

pDC411

pAAV-RC

pAdTrack

pacAd5 CMVK-NpA

pAdEasy-1

pDC315

pAAV-MCS

pShuttle

pHelper

pBApo-CMV-neo

pShuttle-CMV-lacZ

pBApo-CMV

pAAV-TRE-Syn-Fluc

pBApo-CMV-Pur

pAAV-minCMV-mCherry