pAAV-CAG-RFP

pAAV-CAG-RFP

pAAV-CAG-RFP载体基本信息：

pAAV-CAG-RFP载体质粒图谱和多克隆位点信息:

pAAV-CAG-RFP载体简介:

pAAV-CAG-RFP载体

This AAV is a RFP construct driven by the ubiquitous CAG promoter.

The roughly 1.8Kb CAG promoter (also known as CBA promoter or CAGGS promoter) is a strong synthetic promoter frequently used to drive high levels of gene expression in mammalian cells. The CAG promoter is composed of the following regulatory elements: (C) cytomegalovirus (CMV) early enhancer element; (A) the promoter region, the first exon, and the first intron of chicken beta-Actin gene, and (G) the splice acceptor of the rabbit beta-Globin gene. Like the EF1a promoter, the CAG promoter is commonly used as an alternative for the CMV promoter, from which the expression is decreased due to methylation/silencing. In many cell types tested, the CAG and EF1a promoters give much higher levels of expression than other commonly used cellular promoters such as the UBC and PGK promoters.

RFP has an Excitation/Emission spectrum of 500nm/580nm. Other fluorescent proteins with similar spectrums include Kusabira Orange , mOrange, mOrange2, dTomato, tdTomato, DsRed, DsRed2, TurboRFP, and TagRFP

pAAV-CAG-RFP载体序列:

ORIGIN

    1 ATAAAAAGCG AAGCGCGCGG CGGGCGGGAG TCGCTGCGCG CTGCCTTCGC CCCGTGCCCC

   61 GCTCCGCCGC CGCCTCGCGC CGCCCGCCCC GGCTCTGACT GACCGCGTTA CTCCCACAGG

  121 TGAGCGGGCG GGACGGCCCT TCTCCTCCGG GCTGTAATTA GCGCTTGGTT TAATGACGGC

  181 TTGTTTCTTT TCTGTGGCTG CGTGAAAGCC TTGAGGGGCT CCGGGAGGGC CCTTTGTGCG

  241 GGGGGAGCGG CTCGGGGGGT GCGTGCGTGT GTGTGTGCGT GGGGAGCGCC GCGTGCGGCT

  301 CCGCGCTGCC CGGCGGCTGT GAGCGCTGCG GGCGCGGCGC GGGGCTTTGT GCGCTCCGCA

  361 GTGTGCGCGA GGGGAGCGCG GCCGGGGGCG GTGCCCCGCG GTGCGGGGGG GGCTGCGAGG

  421 GGAACAAAGG CTGCGTGCGG GGTGTGTGCG TGGGGGGGTG AGCAGGGGGT GTGGGCGCGT

  481 CGGTCGGGCT GCAACCCCCC CTGCACCCCC CTCCCCGAGT TGCTGAGCAC GGCCCGGCTT

  541 CGGGTGCGGG GCTCCGTACG GGGCGTGGCG CGGGGCTCGC CGTGCCGGGC GGGGGGTGGC

  601 GGCAGGTGGG GGTGCCGGGC GGGGCGGGGC CGCCTCGGGC CGGGGAGGGC TCGGGGGAGG

  661 GGCGCGGCGG CCCCCGGAGC GCCGGCGGCT GTCGAGGCGC GGCGAGCCGC AGCCATTGCC

  721 TTTTATGGTA ATCGTGCGAG AGGGCGCAGG GACTTCCTTT GTCCCAAATC TGTGCGGAGC

  781 CGAAATCTGG GAGGCGCCGC CGCACCCCCT CTAGCGGGCG CGGGGCGAAG CGGTGCGGCG

  841 CCGGCAGGAA GGAAATGGGC GGGGAGGGCC TTCGTGCGTC GCCGCGCCGC CGTCCCCTTC

  901 TCCCTCTCCA GCCTCGGGGC TGTCCGCGGG GGGACGGCTG CCTTCGGGGG GGACGGGGCA

  961 GGGCGGGGTT CGGCTTCTGG CGTGTGACCG GCGGCTCTAG AGCCTCTGCT AACCATGTTC

 1021 ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG GCAACGTGCT GGTTATTGTG CTGTCTCATC

 1081 ATTTTGGCAA AGAATTGTGG CGGCCCGACC GCGGGCTAGC GGCGCGCCGA GCTCGGATCC

 1141 TGAGAACTTC AGGGTGAGTC TATGGGACCC TTGATGTTTT CTTTCCCCTT CTTTTCTATG

 1201 GTTAAGTTCA TGTCATAGGA AGGGGAGAAG TAACAGGGTA CACATATTGA CCAAATCAGG

 1261 GTAATTTTGC ATTTGTAATT TTAAAAAATG CTTTCTTCTT TTAATATACT TTTTTGTTTA

 1321 TCTTATTTCT AATACTTTCC CTAATCTCTT TCTTTCAGGG CAATAATGAT ACAATGTATC

 1381 ATGCCTCTTT GCACCATTCT AAAGAATAAC AGTGATAATT TCTGGGTTAA GGCAATAGCA

 1441 ATATTTCTGC ATATAAATAT TTCTGCATAT AAATTGTAAC TGATGTAAGA GGTTTCATAT

 1501 TGCTAATAGC AGCTACAATC CAGCTACCAT TCTGCTTTTA TTTTATGGTT GGGATAAGGC

 1561 TGGATTATTC TGAGTCCAAG CTAGGCCCTT TTGCTAATCA TGTTCATACC TCTTATCTTC

 1621 CTCCCACAGC TCCTGGGCAA CGTGCTGGTC TGTGTGCTGG CCCATCACTT TGGCAAAGCA

 1681 CGTGAATTCG AAGATCTGGC CGCCTCGGCC TCTAGAACTA GTGGATCCCC CGGGCTGCAG

 1741 GAATTCGATG CTACCGGTCG CCACCATGGC CTCCTCCGAG GACGTCATCA AGGAGTTCAT

 1801 GCGCTTCAAG GTGCGCATGG AGGGCTCCGT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA

 1861 GGGCGAGGGC CGCCCCTACG AGGGCACCCA GACCGCCAAG CTGAAGGTGA CCAAGGGCGG

 1921 CCCCCTGCCC TTCGCCTGGG ACATCCTGTC CCCCCAGTTC CAGTACGGCT CCAAGGTGTA

 1981 CGTGAAGCAC CCCGCCGACA TCCCCGACTA CAAGAAGCTG TCCTTCCCCG AGGGCTTCAA

 2041 GTGGGAGCGC GTGATGAACT TCGAGGACGG CGGCGTGGTG ACCGTGACCC AGGACTCCTC

 2101 CCTGCAGGAC GGCTCCTTCA TCTACAAGGT GAAGTTCATC GGCGTGAACT TCCCCTCCGA

 2161 CGGCCCCGTA ATGCAGAAGA AGACTATGGG CTGGGAGGCC TCCACCGAGC GCCTGTACCC

 2221 CCGCGACGGC GTGCTGAAGG GCGAGATCCA CAAGGCCCTG AAGCTGAAGG ACGGCGGCCA

 2281 CTACCTGGTG GAGTTCAAGT CCATCTACAT GGCCAAGAAG CCCGTGCAGC TGCCCGGCTA

 2341 CTACTACGTG GACTCCAAGC TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTGGA

 2401 GCAGTACGAG CGCGCCGAGG GCCGCCACCA CCTGTTCCTG TAGCGGCCAA ATGAATTCCC

 2461 AAACCGTACG GGAAACGGCC CTATTCTATA GTGTCACCTA AATGCTAGAG CTCGCTGATC

 2521 AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC

 2581 CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC

 2641 GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG

 2701 GGAGGATTGG GAAGACAATA GCGCGGCCGC TCTAGAGCAT GGCTACGTAG ATAAGTAGCA

 2761 TGGCGGGTTA ATCATTAACT ACAAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT

 2821 GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC GCCCGACGCC CGGGCTTTGC

 2881 CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCTGGCGT AATAGCGAAG AGGCCCGCAC

 2941 CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGATTCC GTTGCAATGG

 3001 CTGGCGGTAA TATTGTTCTG GATATTACCA GCAAGGCCGA TAGTTTGAGT TCTTCTACTC

 3061 AGGCAAGTGA TGTTATTACT AATCAAAGAA GTATTGCGAC AACGGTTAAT TTGCGTGATG

 3121 GACAGACTCT TTTACTCGGT GGCCTCACTG ATTATAAAAA CACTTCTCAG GATTCTGGCG

 3181 TACCGTTCCT GTCTAAAATC CCTTTAATCG GCCTCCTGTT TAGCTCCCGC TCTGATTCTA

 3241 ACGAGGAAAG CACGTTATAC GTGCTCGTCA AAGCAACCAT AGTACGCGCC CTGTAGCGGC

 3301 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC

 3361 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC

 3421 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC

 3481 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG

 3541 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT

 3601 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT

 3661 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA

 3721 ATATTAACGC TTACAATTTA AATATTTGCT TATACAATCT TCCTGTTTTT GGGGCTTTTC

 3781 TGATTATCAA CCGGGGTACA TATGATTGAC ATGCTAGTTT TACGATTACC GTTCATCGAT

 3841 TCTCTTGTTT GCTCCAGACT CTCAGGCAAT GACCTGATAG CCTTTGTAGA GACCTCTCAA

 3901 AAATAGCTAC CCTCTCCGGC ATGAATTTAT CAGCTAGAAC GGTTGAATAT CATATTGATG

 3961 GTGATTTGAC TGTCTCCGGC CTTTCTCACC CGTTTGAATC TTTACCTACA CATTACTCAG

 4021 GCATTGCATT TAAAATATAT GAGGGTTCTA AAAATTTTTA TCCTTGCGTT GAAATAAAGG

 4081 CTTCTCCCGC AAAAGTATTA CAGGGTCATA ATGTTTTTGG TACAACCGAT TTAGCTTTAT

 4141 GCTCTGAGGC TTTATTGCTT AATTTTGCTA ATTCTTTGCC TTGCCTGTAT GATTTATTGG

 4201 ATGTTGGAAT CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG

 4261 CATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA

 4321 CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG

 4381 ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA

 4441 ACGCGCGAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT

 4501 AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG

 4561 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT

 4621 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT

 4681 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT

 4741 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG

 4801 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA

 4861 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG

 4921 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT

 4981 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC

 5041 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA

 5101 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT

 5161 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT

 5221 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC

 5281 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA

 5341 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG

 5401 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG

 5461 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA

 5521 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA

 5581 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA

 5641 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG

 5701 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA

 5761 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA

 5821 TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC

 5881 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG

 5941 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC

 6001 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT

 6061 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC

 6121 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG

 6181 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG

 6241 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT

 6301 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA

 6361 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG

 6421 CAGCGAGTCA GTGAGCGAGC GAGCGCGCAG AGAGGGAGTG GCCAACTCCA TCACTAGGGG

 6481 TTCCTTGTAG TTAATGATTA ACCCGCCATG CTACTTATCT ACGTAGCCAT GCTCTAGAGG

 6541 ATCCTTCGAA AAGCTTGTAT ACGGCCGGCC ACGCGTTCGC CACTCGACAT TGATTATTGA

 6601 CTAGTTATTA ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC

 6661 GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT

 6721 TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC

 6781 AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC

 6841 CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT

 6901 ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA

 6961 CCATGGTCGA GGTGAGCCCC ACGTTCTGCT TCACTCTCCC CATCTCCCCC CCCTCCCCAC

 7021 CCCCAATTTT GTATTTATTT ATTTTTTAAT TATTTTGTGC AGCGATGGGG GCGGGGGGGG

 7081 GGGGGGGGCG CGCGCCAGGC GGGGCGGGGC GGGGCGAGGG GCGGGGCGGG GCGAGGCGGA

 7141 GAGGTGCGGC GGCAGCCAAT CAGAGCGGCG CGCTCCGAAA GTTTCCTTTT ATGGCGAGGC

 7201 GGCGGCGGCG GCGGCCCT

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pAAV-CAG-RFP其他腺病毒表达载体：