北京华越洋生物
13581845453(微信)
7*24h全国热线:
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首页    载体及质粒    Tet-pLKO-puro
1

Tet-pLKO-puro

Tet-pLKO-puro

Tet-pLKO-puro


 

编号

载体名称

北京华越洋VECT231142

Tet-pLKO-puro

 

Tet-pLKO-puro载体基本信息:

载体名称:

Tet-pLKO-puro

质粒类型:

慢病毒载体;RNAi载体;四环素诱导载体;Tet-On载体

高拷贝/低拷贝:

低拷贝

克隆方法:

AgeIEcoRI

启动子:

CMV

载体大小:

10633   bp

5' 测序引物及序列:

5’   GGCAGGGATATTCACCATTATCGTTTCAGA 3’

3' 测序引物及序列:

--

载体标签:

--

载体抗性:

氨苄青霉素

筛选标记:

Puromycin

克隆菌株:

Stbl3 感受态细胞 ,以维持质粒的稳定性。

宿主细胞(系):

哺乳动物细胞

备注:

其它筛选标记 Tet-pLKO-neo

稳定性:

稳表达

组成型/诱导型:

诱导型

病毒/非病毒:

慢病毒

 

Tet-pLKO-puro载体质粒图谱和多克隆位点信息:

Tet-pLKO-puro载体序列

ORIGIN

    1 TTGGGGTTGC GCCTTTTCCA AGGCAGCCCT GGGTTTGCGC AGGGACGCGG CTGCTCTGGG

   61 CGTGGTTCCG GGAAACGCAG CGGCGCCGAC CCTGGGTCTC GCACATTCTT CACGTCCGTT

  121 CGCAGCGTCA CCCGGATCTT CGCCGCTACC CTTGTGGGCC CCCCGGCGAC GCTTCCTGCT

  181 CCGCCCCTAA GTCGGGAAGG TTCCTTGCGG TTCGCGGCGT GCCGGACGTG ACAAACGGAA

  241 GCCGCACGTC TCACTAGTAC CCTCGCAGAC GGACAGCGCC AGGGAGCAAT GGCAGCGCGC

  301 CGACCGCGAT GGGCTGTGGC CAATAGCGGC TGCTCAGCAG GGCGCGCCGA GAGCAGCGGC

  361 CGGGAAGGGG CGGTGCGGGA GGCGGGGTGT GGGGCGGTAG TGTGGGCCCT GTTCCTGCCC

  421 GCGCGGTGTT CCGCATTCTG CAAGCCTCCG GAGCGCACGT CGGCAGTCGG CTCCCTCGTT

  481 GACCGAATCA CCGACCTCTC TCCCCAGGGG GATCTGTGAG TTTGGGGACC CTTGATTGTT

  541 CTTTCTTTTT CGCTATTGTA AAATTCATGT TATATGGAGG GGGCAAAGTT TTCAGGGTGT

  601 TGTTTAGAAT GGGAAGATGT CCCTTGTATC ACCATGGACC CTCATGATAA TTTTGTTTCT

  661 TTCACTTTCT ACTCTGTTGA CAACCATTGT CTCCTCTTAT TTTCTTTTCA TTTTCTGTAA

  721 CTTTTTCGTT AAACTTTAGC TTGCATTTGT AACGAATTTT TAAATTCACT TTTGTTTATT

  781 TGTCAGATTG TAAGTACTTT CTCTAATCAC TTTTTTTTCA AGGCAATCAG GGTATATTAT

  841 ATTGTACTTC AGCACAGTTT TAGAGAACAA TTGTTATAAT TAAATGATAA GGTAGAATAT

  901 TTCTGCATAT AAATTCTGGC TGGCGTGGAA ATATTCTTAT TGGTAGAAAC AACTACATCC

  961 TGGTCATCAT CCTGCCTTTC TCTTTATGGT TACAATGATA TACACTGTTT GAGATGAGGA

 1021 TAAAATACTC TGAGTCCAAA CCGGGCCCCT CTGCTAACCA TGTTCATGCC TTCTTCTTTT

 1081 TCCTACAGCT CCTGGGCAAC GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGAAT

 1141 TGTAATACGA CTCACTATAG GGCGAATTGA TATGTCTAGA TTAGATAAAA GTAAAGTGAT

 1201 TAACAGCGCA TTAGAGCTGC TTAATGAGGT CGGAATCGAA GGTTTAACAA CCCGTAAACT

 1261 CGCCCAGAAG CTAGGTGTAG AGCAGCCTAC ATTGTATTGG CATGTAAAAA ATAAGCGGGC

 1321 TTTGCTCGAC GCCTTAGCCA TTGAGATGTT AGATAGGCAC CATACTCACT TTTGCCCTTT

 1381 AGAAGGGGAA AGCTGGCAAG ATTTTTTACG TAATAACGCT AAAAGTTTTA GATGTGCTTT

 1441 ACTAAGTCAT CGCGATGGAG CAAAAGTACA TTTAGGTACA CGGCCTACAG AAAAACAGTA

 1501 TGAAACTCTC GAAAATCAAT TAGCCTTTTT ATGCCAACAA GGTTTTTCAC TAGAGAATGC

 1561 ATTATATGCA CTCAGCGCTG TGGGGCATTT TACTTTAGGT TGCGTATTGG AAGATCAAGA

 1621 GCATCAAGTC GCTAAAGAAG AAAGGGAAAC ACCTACTACT GATAGTATGC CGCCATTATT

 1681 ACGACAAGCT ATCGAATTAT TTGATCACCA AGGTGCAGAG CCAGCCTTCT TATTCGGCCT

 1741 TGAATTGATC ATATGCGGAT TAGAAAAACA ACTTAAATGT GAAAGTGGGT CCGCGTACAG

 1801 CGGCTCCCGG GAGTTCTAGG GATCTGCCCC TCTCCCTCCC CCCCCCCTAA CGTTACTGGC

 1861 CGAAGCCGCT TGGAATAAGG CCGGTGTGCG TTTGTCTATA TGTTATTTTC CACCATATTG

 1921 CCGTCTTTTG GCAATGTGAG GGCCCGGAAA CCTGGCCCTG TCTTCTTGAC GAGCATTCCT

 1981 AGGGGTCTTT CCCCTCTCGC CAAAGGAATG CAAGGTCTGT TGAATGTCGT GAAGGAAGCA

 2041 GTTCCTCTGG AAGCTTCTTG AAGACAAACA ACGTCTGTAG CGACCCTTTG CAGGCAGCGG

 2101 AACCCCCCAC CTGGCGACAG GTGCCTCTGC GGCCAAAAGC CACGTGTATA AGATACACCT

 2161 GCAAAGGCGG CACAACCCCA GTGCCACGTT GTGAGTTGGA TAGTTGTGGA AAGAGTCAAA

 2221 TGGCTCTCCT CAAGCGTATT CAACAAGGGG CTGAAGGATG CCCAGAAGGT ACCCCATTGT

 2281 ATGGGATCTG ATCTGGGGCC TCGGTGCACA TGCTTTACAT GTGTTTAGTC GAGGTTAAAA

 2341 AAACGTCTAG GCCCCCCGAA CCACGGGGAC GTGGTTTTCC TTTGAAAAAC ACGATGATAA

 2401 GGATCCACCG GAGCTTACCA TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA

 2461 CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG

 2521 CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT

 2581 CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC

 2641 GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG

 2701 CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC

 2761 GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA

 2821 CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC

 2881 CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT

 2941 CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT GGTGCATGAC

 3001 CCGCAAGCCC GGTGCCTGAC GCCCGCCCCA CGACCCGCAG CGCCCGACCG AAAGGAGCGC

 3061 ACGACCCCAT GCATCGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG

 3121 CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAGACAAGA

 3181 TCTGCTTTTT GCTTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC

 3241 TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT

 3301 AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC

 3361 AGTGTGGAAA ATCTCTAGCA GTAGTAGTTC ATGTCATCTT ATTATTCAGT ATTTATAACT

 3421 TGCAAAGAAA TGAATATCAG AGAGTGAGAG GAACTTGTTT ATTGCAGCTT ATAATGGTTA

 3481 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG

 3541 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGCTCTAGC TATCCCGCCC

 3601 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC

 3661 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG

 3721 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGGACGTACC CAATTCGCCC TATAGTGAGT

 3781 CGTATTACGC GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG

 3841 TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG

 3901 AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGGACGCGC

 3961 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC

 4021 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG

 4081 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT

 4141 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC

 4201 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT

 4261 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA

 4321 TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA

 4381 ATTTTAACAA AATATTAACG CTTACAATTT AGGTGGCACT TTTCGGGGAA ATGTGCGCGG

 4441 AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA

 4501 ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG

 4561 TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC

 4621 GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT

 4681 GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT

 4741 GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA

 4801 GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC

 4861 AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT

 4921 GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC

 4981 CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT

 5041 GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC

 5101 GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA

 5161 CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG

 5221 GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT

 5281 GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC

 5341 TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA

 5401 ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT

 5461 TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA

 5521 GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC

 5581 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT

 5641 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC

 5701 GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC

 5761 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG

 5821 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG

 5881 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA

 5941 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC

 6001 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG

 6061 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG

 6121 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT

 6181 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC

 6241 TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG

 6301 AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC

 6361 GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG

 6421 GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA

 6481 GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT

 6541 TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCGC GCAATTAACC CTCACTAAAG

 6601 GGAACAAAAG CTGGAGCTGC AAGCTTAATG TAGTCTTATG CAATACTCTT GTAGTCTTGC

 6661 AACATGGTAA CGATGAGTTA GCAACATGCC TTACAAGGAG AGAAAAAGCA CCGTGCATGC

 6721 CGATTGGTGG AAGTAAGGTG GTACGATCGT GCCTTATTAG GAAGGCAACA GACGGGTCTG

 6781 ACATGGATTG GACGAACCAC TGAATTGCCG CATTGCAGAG ATATTGTATT TAAGTGCCTA

 6841 GCTCGATACA TAAACGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC

 6901 TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG

 6961 TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG

 7021 TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG AAACCAGAGG

 7081 AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC GAGGGGCGGC

 7141 GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA GATGGGTGCG

 7201 AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT CGGTTAAGGC

 7261 CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG GAGCTAGAAC

 7321 GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA ATACTGGGAC

 7381 AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT AATACAGTAG

 7441 CAACCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA GCTTTAGACA

 7501 AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGCGGCC GCTGATCTTC

 7561 AGACCTGGAG GAGGAGATAT GAGGGACAAT TGGAGAAGTG AATTATATAA ATATAAAGTA

 7621 GTAAAAATTG AACCATTAGG AGTAGCACCC ACCAAGGCAA AGAGAAGAGT GGTGCAGAGA

 7681 GAAAAAAGAG CAGTGGGAAT AGGAGCTTTG TTCCTTGGGT TCTTGGGAGC AGCAGGAAGC

 7741 ACTATGGGCG CAGCGTCAAT GACGCTGACG GTACAGGCCA GACAATTATT GTCTGGTATA

 7801 GTGCAGCAGC AGAACAATTT GCTGAGGGCT ATTGAGGCGC AACAGCATCT GTTGCAACTC

 7861 ACAGTCTGGG GCATCAAGCA GCTCCAGGCA AGAATCCTGG CTGTGGAAAG ATACCTAAAG

 7921 GATCAACAGC TCCTGGGGAT TTGGGGTTGC TCTGGAAAAC TCATTTGCAC CACTGCTGTG

 7981 CCTTGGAATG CTAGTTGGAG TAATAAATCT CTGGAACAGA TTTGGAATCA CACGACCTGG

 8041 ATGGAGTGGG ACAGAGAAAT TAACAATTAC ACAAGCTTAA TACACTCCTT AATTGAAGAA

 8101 TCGCAAAACC AGCAAGAAAA GAATGAACAA GAATTATTGG AATTAGATAA ATGGGCAAGT

 8161 TTGTGGAATT GGTTTAACAT AACAAATTGG CTGTGGTATA TAAAATTATT CATAATGATA

 8221 GTAGGAGGCT TGGTAGGTTT AAGAATAGTT TTTGCTGTAC TTTCTATAGT GAATAGAGTT

 8281 AGGCAGGGAT ATTCACCATT ATCGTTTCAG ACCCACCTCC CAACCCCGAG GGGACCCGAC

 8341 AGGCCCGAAG GAATAGAAGA AGAAGGTGGA GAGAGAGACA GAGACAGATC CATTCGATTA

 8401 GTGAACGGAT CTCGACGGTA TCGATCACGA GACTAGCCTC GAGCGGCCGC AATATTTGCA

 8461 TGTCGCTATG TGTTCTGGGA AATCACCATA AACGTGAAAT CCCTATCAGT GATAGAGACT

 8521 TATAAGTTCC CTATCAGTGA TAGAGACACC GGTGTGTTGT AAATGAGCAC ACAAAATACA

 8581 CATGCTAAAA TATTATATTC TATGACCTTT ATAAAATCAA CCAAAATCTT CTTTTTAATA

 8641 ACTTTAGTAT CAATAATTAG AATTTTTATG TTCCTTTTTG CAAACTTTTA ATAAAAATGA

 8701 GCAAAATAAA AAAACGCTAG TTTTAGTAAC TCGCGTTGTT TTCTTCACCT TTAATAATAG

 8761 CTACTCCACC ACTTGTTCCT AAGCGGTCAG CTCCTGCTTC AATCATTTTT TGAGCATCTT

 8821 CAAATGTTCT AACTCCACCA GCTGCTTTAA CTAAAGCATT GTCTTTAACA ACTGACTTCA

 8881 TTAGTTTAAC ATCTTCAAAT GTTGCACCTG ATTTTGAAAA TCCTGTTGAT GTTTTAACAA

 8941 ATTCTAATCC AGCTTCAACA GCTATTTCAC AAGCTTTCAT GATTTCTTCT TTTGTTAATA

 9001 AACAATTTTC CATAATACAT TTAACAACAT GTGATCCAGC TGCTTTTTTT ACAGCTTTCA

 9061 TGTCTTCTAA AACTAATTCA TAATTTTTGT CTTTTAATGC ACCAATATTT AATACCATAT

 9121 CAATTTCTGT TGCACCATCT TTAATTGCTT CAGAAACTTC GAATGCTTTT GTAGCTGTTG

 9181 TGCATGCACC TAGAGGAAAA CCTACAACAT TTGTTATTCC TACATTTGTG CCTTTTAATA

 9241 ATTCTTTACA ATAGCTTGTT CAATATGAAT TAACACAAAC TGTTGCAAAA TCAAATTCAA

 9301 TTGCTTCATC ACATAATTGT TTAATTTCAG CTTTCGTAGC ATCTTGTTTT AATAATGTGT

 9361 GATCTATATA TTTGTTTAGT TTCATTTTTT CTCCTATATA TTCATTTTTA ATTTTAATTC

 9421 TTTAATAATT TCGTCTACTT TAACTTTAGC GTTTTGAACA GATTCACCAA CACCTATAAA

 9481 ATAAATTTTT AGTTTAGGTT CAGTTCCACT TGGGCGAACA GCAAATCATG ACTTATCTTC

 9541 TAAATAAAAT TTTAGTAAGT CTTGTCCTGG CATATTATAC ATTCCATCGA TGTAGTCTTC

 9601 AACATTAACA ACTTTAAGTC CAGCAATTTG AGTTAAGGGT GTTGCTCTCA ATGATTTCAT

 9661 TAATGGTTCA ATTTTTAATT TCTTTTCTTC TGGTTTAAAA TTCAAGTTTA AAGTGAAAGT

 9721 GTAATATGCA CCCATTTCTT TAAATAAATC TTCTAAATAG TCTACTAATG TTTTATTTTG

 9781 TTTTTTATAA AATCAAGCAG CCTCTGCTAT TAATATAGAA GCTTGTATTC CATCTTTATC

 9841 TCTAGCTGAG TCATCAATTA CATATCCATA ACTTTCTTCA TAAGCAAAAA CAAAATTTAA

 9901 TCCGTTATCT TCTTCTTTAG CAATTTCTCT ACCCATTCAT TTAAATCCAG TTAAAGTTTT

 9961 TACAATATTA ACTCCATATT TTTCATGAGC GATTCTATCA CCCAAATCAC TTGTTACAAA

10021 ACTTGAATAT AGAGCCGGAT TTTTTGGAAT GCTATTTAAG CGTTTTAGAT TTGATAATTT

10081 TCAATCAATT AAAATTGGTC CTGTTTGATT TCCATCTAAT CTTACAAAAT GACCATCATG

10141 TTTTATTGCC ATTCCAAATC TGTCAGCATC TGGGTCATTC ATAATAATAA TATCTGCATC

10201 ATGTTTAATA CCATATTCAA GCGGTATTTT TCATGCAGGA TCAAATTCTG GATTTGGATT

10261 TACAACATTT TTAAATGTTT CATCTTCAAA TGCATGCTCT TCAACCTCAA TAACGTTATA

10321 TCCTGATTCA CGTAATATTT TTGGGGTAAA TTTAGTTCCT GTTCCATTAA CTGCGCTAAA

10381 AATAATTTTT AAATCTTTTT TAGCTTCTTG CTCTTTTTTG TAGAATTCTC GACCTCGAGA

10441 CAAATGGCAG TATTCATCCA CAATTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA

10501 GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA

10561 ATTACAAAAA TTCAAAATTT TCGGGTTTAT TACAGGGACA GCAGAGATCC ACTTTGGCCG

10621 CGGCTCGAGG GGG

//

 

Tet-pLKO-puro其他相关慢病毒载体:

pLVX-DsRed-Monomer-N1

pLVX-PAmCherry-C1

pLVX-IRES-mCherry

Tet-pLKO-neo

pLVX-DsRed-Express2-C1

pLVX-tdTomato-C1

pLVX-mCherry-C1

pLVX-AcGFP1-N1

pLVX-Tet-On-Advanced

pLKO.1-puro

FUW-tetO-hSOX2

pLVX-IRES-Hyg

pLVX-IRES-Puro

pCDH-MSCV-MCS-EF1-copGFP-T2A-Puro

pLVX-EF1α-AcGFP1-C1

pLVX-EF1α-DsRed-Monomer-C1

pLVX-MetLuc

pLVX-EF1α-IRES-ZsGreen1

pLVX-EF1α-mCherry-C1

pLVX-EF1α-IRES-mCherry

pLVX-Hom-Mem1

pLVX-MetLuc     Control

pLVX-EF1α-AcGFP1-N1

pCDH-CMV-MCS-EF1-Puro

pLVX-Het-2

pLVX-IRES-Neo

pPRIME-TET-GFP-FF3

pSIH1-H1-CopGFP

pLentilox     3.7

pLOX-CW-CRE

pRSV-rev

pLVX-DD-AmCyan1     Reporter

pLL3.7

pLVX-Het-1

pMDLg-pRRE

pLVX-DD-tdTomato     Reporter

pLVX-PTuner

pLVX-PTuner-Green

pLVX-CherryPicker2

pLVX-rHom-1

pLOX-CWBmi1

pLVX-TetOne-Puro

pLVX-TetOne-Luc

pCDH-EF1-MCS-T2A-Puro

pLVX-rHom-Sec1

pLVX-TetOne-Puro-Luc

pLVX-DD-AcGFP1-Actin

pLVX-Het-Nuc1

pLVX-DD-tdTomato     Control

pLVX-TetOne

pLVX-DD-ZsGreen1     Reporter

pLVX-DD-AmCyan1     Control

pLVX-rHom-Nuc1

pCDH-CMV-MCS-EF1-Neo

pLenti6.3-MCS-IRES2-EGFP

pLVX-Hom-Nuc1

pCDF1-MCS2-EF1-Puro

pLenti6.3/V5-GW/EmGFP

pLVX-PTuner2

pLVX-TRE3G-ZsGreen1

pLVX-TRE3G-mCherry

pLVX-CherryPicker     Control

pLenti6/V5-GW/lacZ

pCDH-EF1-MCS-T2A-copGFP

pCDH-CMV-MCS-EF1-Hygro

pLenti6.3-DsRed2-BveI     miR

pCDH-MCS-T2A-Puro-MSCV

pCDH1-MCS2-EF1-copGFP

psPAX2

pCDH-CMV-MCS-EF1-RFP-T2A-Puro

pWPXL

pcDNA6.2-DsRed2-MCS1     miR

FUGW

pLenti6.3-EmGFP-BveI     miR

pcDNA6.2-EmGFP-BsaI     miR

pGIPZ

pLenti6.3-MCS

pLEX-MCS

pLenti6.3-BveI     miR

pSicoR

pLP1

pcDNA6.3-EmGFP-NC-     II

pLVX-TRE3G-IRES

pLOX-Ttag-iresTK

VSV-G

pLentG-KOSM

pCMV-dR8.91

pMDLg/pRRE

pFUGW

pCgpv

pLVX-TRE3G-Luc     Control

pLP2

pLVTHM

pSico

pLenti6.3-MCS-IRES2-DsRed2

pPACKH1-REV

pGensil-1

pcDNA6.2-EmGFP-NC-     I

pLKO.1-puro-GFP-siRNA

FUW-tetO-hOCT4

pSico     PGK Puro

FUW-tetO-hMYC

FUW

pLVX-shRNA2

FUW-M2rtTA

pCDH-MSCV-MCS-EF1-copGFP

pLKO.1-GFP-shRNA

pLVX-AmCyan1-C1

pLKO.1-TRC     control

pPACKH1-GAG

pLVX-DsRed-Monomer-C1

pMD2.G

pLKO.1-TRC

pLVX-ZsGreen1-C1

pLVX-AmCyan1-N1

FUW-tetO-hOKMS

pLVX-IRES-tdTomato

pLKO.1-hygro

FUW-tetO-hKLF4

pLVX-tdTomato-N1

pCMV-dR8.2-dvpr

pLVX-AcGFP1-C1

pLVX-Tight-Puro

Tet-pLKO-puro

pLVX-DsRed-Express2-N1

pLVX-EF1α-mCherry-N1

pLVX-mCherry-N1

pLVX-PAmCherry-N1

pLVX-EF1α-IRES-Puro

pLVX-IRES-ZsGreen1

pLVX-PTuner2-C

pLVX-Het-Mem1

pPRIME-TREX-GFP-FF3

pLVX-DD-ZsGreen1     Control

pLP/VSVG

pCDH-CMV-MCS-EF1-copGFP

pCDH-UbC-MCS-EF1-Hygro

pcDNA6.2-EmGFP-MCS1     miR

pLOX-TERT-iresTK

pCDH-CMV-MCS-EF1-RFP

pcDNA6.2-BsaI     miR

pCDH-EF1-MCS-(PGK-Puro)

pCDF1-MCS2-EF1-copGFP

pTRIPZ

pLVX-Hom-1

pLVX-shRNA1

LeGO-iC2

pLVX-mCherry-Actin

pcDNA6.2-DsRed2-BsmBI     miR

pLKO.3G

pLVX-CherryPicker1

pLVX-TRE3G

pLVX-Puro

pCDH-CMV-MCS-EF1-copGFP-T2A-Puro

pSicoR     PGK Puro

pCMV-DsRed-Express2

pLVX-TRE3G-Hom1