pLOX-CWBmi1
pLOX-CWBmi1
编号 | 载体名称 |
北京华越洋VECT2311100 | pLOX-CWBmi1 |
pLOX-CWBmi1载体基本信息：
载体名称: | pLOX-CWBmi1 |
质粒类型: | 慢病毒系统载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | -- |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 9967bp |
5' 测序引物及序列: | CMV fwd: CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | SP6: ATTTAGGTGACACTATAG |
载体标签: | -- |
载体抗性: | 氨苄青霉素（Ampicillian） |
筛选标记: | -- |
备注: | 克隆菌株为Stbl3或 HB101。 |
稳定性: | -- |
组成型: | -- |
病毒/非病毒: | 慢病毒 |
pLOX-CWBmi1载体质粒图谱和多克隆位点信息：
pLOX-CWBmi1载体简介：
pLOX-CWBmi1载体序列：
ORIGIN
1 AGTTGGAAGG GCTAATTCAC TCCCAAAGAA GACAAGATAT CCTTGATCTG TGGATCTACC
61 ACACACAAGG CTACTTCCCT GATTAGCAGA ACTACACACC AGGGCCAGGG GTCAGATATC
121 CACTGACCTT TGGATGGTGC TACAAGCTAG TACCAGTTGA GCCAGATAAG GTAGAAGAGG
181 CCAATAAAGG AGAGAACACC AGCTTGTTAC ACCCTGTGAG CCTGCATGGG ATGGATGACC
241 CGGAGAGAGA AGTGTTAGAG TGGAGGTTTG ACAGCCGCCT AGCATTTCAT CACGTGGCCC
301 GAGAGCTGCA TCCGGAGTAC TTCAAGAACT GCTGATATCG AGCTTGCTAC AAGGGACTTT
361 CCGCTGGGGA CTTTCCAGGG AGGCGTGGCC TGGGCGGGAC TGGGGAGTGG CGAGCCCTCA
421 GATCCTGCAT ATAAGCAGCT GCTTTTTGCC TGTACTGGGT CTCTCTGGTT AGACCAGATC
481 TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG
541 CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC
601 CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGTG GCGCCCGAAC AGGGACTTGA
661 AAGCGAAAGG GAAACCAGAG GAGCTCTCTC GACGCAGGAC TCGGCTTGCT GAAGCGCGCA
721 CGGCAAGAGG CGAGGGGCGG CGACTGGTGA GTACGCCAAA AATTTTGACT AGCGGAGGCT
781 AGAAGGAGAG AGATGGGTGC GAGAGCGTCA GTATTAAGCG GGGGAGAATT AGATCGCGAT
841 GGGAAAAAAT TCGGTTAAGG CCAGGGGGAA AGAAAAAATA TAAATTAAAA CATATAGTAT
901 GGGCAAGCAG GGAGCTAGAA CGATTCGCAG TTAATCCTGG CCTGTTAGAA ACATCAGAAG
961 GCTGTAGACA AATACTGGGA CAGCTACAAC CATCCCTTCA GACAGGATCA GAAGAACTTA
1021 GATCATTATA TAATACAGTA GCAACCCTCT ATTGTGTGCA TCAAAGGATA GAGATAAAAG
1081 ACACCAAGGA AGCTTTAGAC AAGATAGAGG AAGAGCAAAA CAAAAGTAAG ACCACCGCAC
1141 AGCAAGCGGC CGCTGATCTT CAGACCTGGA GGAGGAGATA TGAGGGACAA TTGGAGAAGT
1201 GAATTATATA AATATAAAGT AGTAAAAATT GAACCATTAG GAGTAGCACC CACCAAGGCA
1261 AAGAGAAGAG TGGTGCAGAG AGAAAAAAGA GCAGTGGGAA TAGGAGCTTT GTTCCTTGGG
1321 TTCTTGGGAG CAGCAGGAAG CACTATGGGC GCAGCGTCAA TGACGCTGAC GGTACAGGCC
1381 AGACAATTAT TGTCTGGTAT AGTGCAGCAG CAGAACAATT TGCTGAGGGC TATTGAGGCG
1441 CAACAGCATC TGTTGCAACT CACAGTCTGG GGCATCAAGC AGCTCCAGGC AAGAATCCTG
1501 GCTGTGGAAA GATACCTAAA GGATCAACAG CTCCTGGGGA TTTGGGGTTG CTCTGGAAAA
1561 CTCATTTGCA CCACTGCTGT GCCTTGGAAT GCTAGTTGGA GTAATAAATC TCTGGAACAG
1621 ATTTGGAATC ACACGACCTG GATGGAGTGG GACAGAGAAA TTAACAATTA CACAAGCTTA
1681 ATACACTCCT TAATTGAAGA ATCGCAAAAC CAGCAAGAAA AGAATGAACA AGAATTATTG
1741 GAATTAGATA AATGGGCAAG TTTGTGGAAT TGGTTTAACA TAACAAATTG GCTGTGGTAT
1801 ATAAAATTAT TCATAATGAT AGTAGGAGGC TTGGTAGGTT TAAGAATAGT TTTTGCTGTA
1861 CTTTCTATAG TGAATAGAGT TAGGCAGGGA TATTCACCAT TATCGTTTCA GACCCACCTC
1921 CCAACCCCGA GGGGACCCGA CAGGCCCGAA GGAATAGAAG AAGAAGGTGG AGAGAGAGAC
1981 AGAGACAGAT CCATTCGATT AGTGAACGGA TCTCGACGGT ATCGATAAGC TTGGGAGTTC
2041 CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA
2101 TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT
2161 CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG
2221 CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG
2281 TACATGACCT TACGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT
2341 ACCATGGTGA TGCGGTTTTG GCAGTACACC AATGGGCGTG GATAGCGGTT TGACTCACGG
2401 GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA CCAAAATCAA
2461 CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG CGGTAGGCGT
2521 GTACGGTGGG AGGTCTATAT AAGCAGAGCT CGTTTAGTGA ACCGTCAGAT CGCCTGGAGA
2581 CGCCATCCAC GCTGTTTTGA CCTCCATAGA AGACACCGAC TCTAGCTAGA GGATCTGATA
2641 TCATCGATGA ATTCTTTCCG GGATCTTTTA TCAAGCAGAA ATGCATCGAA CAACCAGAAT
2701 CAAGATCACT GAGCTAAATC CCCACTTAAT GTGTGTCCTG TGTGGAGGGT ACTTCATTGA
2761 TGCCACTACC ATAATAGAAT GTCTACATTC CTTCTGTAAA ACATGTATTG TACGTTACTT
2821 GGAGACCAGC AAGTATTGTC CTATTTGTGA TGTCCAGGTT CACAAAACCA GACCACTCCT
2881 GAACATAAGG TCAGATAAAA CTCTTCAAGA TATTGTATAC AAATTAGTCC CAGGGCTTTT
2941 CAAAAATGAG ATGAAGAGAA GAAGAGATTT TTATGCAGCT CACCCGTCAG CTGATGCTGC
3001 CAATGGCTCC AATGAAGACC GAGGAGAAGT TGCAGATGAG GAGAAGAGGA TTATAACTGA
3061 TGATGAGATA ATAAGCTTGT CTATTGAGTT CTTTGATCAG AGCAGATTGG ATCGGAAAGT
3121 AAATAAAGAG AAGCCTAAGG AAGAGGTGAA TGATAAAAGG TACTTACGAT GCCCAGCAGC
3181 AATGACTGTG ATGCACTTGA GAAAGTTTCT CAGGAGTAAA ATGGACATAC CCAATACTTT
3241 CCAGATTGAC GTCATGTATG AAGAGGAACC TTTAAAGGAT TACTACACGC TAATGGACAT
3301 TGCCTACATT TATACCTGGA GAAGAAATGG CCCACTACCT TTGAAATACA GAGTTCGGCC
3361 AACTTGCAAA AGAATGAAGA TGAGTCACCA GAGGGATGGA CTGACGAATG CTGGAGAGCT
3421 GGAAAGTGAC TCTGGGAGTG ACAAGGCCAA CAGCCCAGCC GGAGGTGTTC CCTCCACCTC
3481 TTCCTGTTTG CCCAGTCCCA GCACTCCAGT GCAGTCTCCT CACCCACAGT TCCCTCACAT
3541 TTCCAGTACC ATGAATGGAA CCAGCAACAG CCCCAGTGCT AACCACCAAT CTTCCTTTGC
3601 CAGTAGACCT CGAAAATCAT CACTAAATGG GTCATCAGCA ACTTCATCTG GTTAGGACTG
3661 TTAAGGAAAA GATTTTTCAA CCCCCTGATT TAGTTACCTT CATTCATTAC AGCTTTATAG
3721 ATGCTTAATA CATGTGACTG TCGTCCAGTT TGCTTCCTTT TGTAGTGACT TTAAATTTCT
3781 GCTCGAGGGA ATTCCGATAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT
3841 ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT
3901 CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG
3961 TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT
4021 GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT
4081 TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC
4141 TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG
4201 TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC
4261 TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG
4321 CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC
4381 TCCCCGCATC GGGAATTCGA GCTCGGTACC TTTAAGACCA ATGACTTACA AGGCAGCTGT
4441 AGATCTTAGC CACTTTTTAA AAGAAAAGGG GGGACTGGAA GGGCTAATTC ACTCCCAACG
4501 AAGACAAGAT GGGATCAATT CACCATGGGA ATAACTTCGT ATAGCATACA TTATACGAAG
4561 TTATGCTGCT TTTTGCTTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC
4621 TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC
4681 AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT
4741 AGTCAGTGTG GAAAATCTCT AGCAGCATCT AGAATTAATT CCGTGTATTC TATAGTGTCA
4801 CCTAAATCGT ATGTGTATGA TACATAAGGT TATGTATTAA TTGTAGCCGC GTTCTAACGA
4861 CAATATGTAC AAGCCTAATT GTGTAGCATC TGGCTTACTG AAGCAGACCC TATCATCTCT
4921 CTCGTAAACT GCCGTCAGAG TCGGTTTGGT TGGACGAACC TTCTGAGTTT CTGGTAACGC
4981 CGTCCCGCAC CCGGAAATGG TCAGCGAACC AATCAGCAGG GTCATCGCTA GCCAGATCCT
5041 CTACGCCGGA CGCATCGTGG CCGGCATCAC CGGCGCCACA GGTGCGGTTG CTGGCGCCTA
5101 TATCGCCGAC ATCACCGATG GGGAAGATCG GGCTCGCCAC TTCGGGCTCA TGAGCGCTTG
5161 TTTCGGCGTG GGTATGGTGG CAGGCCCCGT GGCCGGGGGA CTGTTGGGCG CCATCTCCTT
5221 GCATGCACCA TTCCTTGCGG CGGCGGTGCT CAACGGCCTC AACCTACTAC TGGGCTGCTT
5281 CCTAATGCAG GAGTCGCATA AGGGAGAGCG TCGAATGGTG CACTCTCAGT ACAATCTGCT
5341 CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC
5401 GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA
5461 TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG ACGAAAGGGC CTCGTGATAC
5521 GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT
5581 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT
5641 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA
5701 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG
5761 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC
5821 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG
5881 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC
5941 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG
6001 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT
6061 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG
6121 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG
6181 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC
6241 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT
6301 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT
6361 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC
6421 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA
6481 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT
6541 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT
6601 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA
6661 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA
6721 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC
6781 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG
6841 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG
6901 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC
6961 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT
7021 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG
7081 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC
7141 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC
7201 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC
7261 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA
7321 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT
7381 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG
7441 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG
7501 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGTG
7561 GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA
7621 AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG
7681 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC
7741 GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT
7801 TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG
7861 AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTT GGACACAAGA CAGGCTTGCG
7921 AGATATGTTT GAGAATACCA CTTTATCCCG CGTCAGGGAG AGGCAGTGCG TAAAAAGACG
7981 CGGACTCATG TGAAATACTG GTTTTTAGTG CGCCAGATCT CTATAATCTC GCGCAACCTA
8041 TTTTCCCCTC GAACACTTTT TAAGCCGTAG ATAAACAGGC TGGGACACTT CACATGAGCG
8101 AAAAATACAT CGTCACCTGG GACATGTTGC AGATCCATGC ACGTAAACTC GCAAGCCGAC
8161 TGATGCCTTC TGAACAATGG AAAGGCATTA TTGCCGTAAG CCGTGGCGGT CTGTACCGGG
8221 TGCGTTACTG GCGCGTGAAC TGGGTATTCG TCATGTCGAT ACCGTTTGTA TTTCCAGCTA
8281 CGATCACGAC AACCAGCGCG AGCTTAAAGT GCTGAAACGC GCAGAAGGCG ATGGCGAAGG
8341 CTTCATCGTT ATTGATGACC TGGTGGATAC CGGTGGTACT GCGGTTGCGA TTCGTGAAAT
8401 GTATCCAAAA GCGCACTTTG TCACCATCTT CGCAAAACCG GCTGGTCGTC CGCTGGTTGA
8461 TGACTATGTT GTTGATATCC CGCAAGATAC CTGGATTGAA CAGCCGTGGG ATATGGGCGT
8521 CGTATTCGTC CCGCCAATCT CCGGTCGCTA ATCTTTTCAA CGCCTGGCAC TGCCGGGCGT
8581 TGTTCTTTTT AACTTCAGGC GGGTTACAAT AGTTTCCAGT AAGTATTCTG GAGGCTGCAT
8641 CCATGACACA GGCAAACCTG AGCGAAACCC TGTTCAAACC CCGCTTTAAA CATCCTGAAA
8701 CCTCGACGCT AGTCCGCCGC TTTAATCACG GCGCACAACC GCCTGTGCAG TCGGCCCTTG
8761 ATGGTAAAAC CATCCCTCAC TGGTATCGCA TGATTAACCG TCTGATGTGG ATCTGGCGCG
8821 GCATTGACCC ACGCGAAATC CTCGACGTCC AGGCACGTAT TGTGATGAGC GATGCCGAAC
8881 GTACCGACGA TGATTTATAC GATACGGTGA TTGGCTACCG TGGCGGCAAC TGGATTTATG
8941 AGTGGGCCCC GGATCTTTGT GAAGGAACCT TACTTCTGTG GTGTGACATA ATTGGACAAA
9001 CTACCTACAG AGATTTAAAG CTCTAAGGTA AATATAAAAT TTTTAAGTGT ATAATGTGTT
9061 AAACTACTGA TTCTAATTGT TTGTGTATTT TAGATTCCAA CCTATGGAAC TGATGAATGG
9121 GAGCAGTGGT GGAATGCCTT TAATGAGGAA AACCTGTTTT GCTCAGAAGA AATGCCATCT
9181 AGTGATGATG AGGCTACTGC TGACTCTCAA CATTCTACTC CTCCAAAAAA GAAGAGAAAG
9241 GTAGAAGACC CCAAGGACTT TCCTTCAGAA TTGCTAAGTT TTTTGAGTCA TGCTGTGTTT
9301 AGTAATAGAA CTCTTGCTTG CTTTGCTATT TACACCACAA AGGAAAAAGC TGCACTGCTA
9361 TACAAGAAAA TTATGGAAAA ATATTCTGTA ACCTTTATAA GTAGGCATAA CAGTTATAAT
9421 CATAACATAC TGTTTTTTCT TACTCCACAC AGGCATAGAG TGTCTGCTAT TAATAACTAT
9481 GCTCAAAAAT TGTGTACCTT TAGCTTTTTA ATTTGTAAAG GGGTTAATAA GGAATATTTG
9541 ATGTATAGTG CCTTGACTAG AGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT
9601 TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT
9661 TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA
9721 TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA
9781 TGTATCTTAT CATGTCTGGA TCAACTGGAT AACTCAAGCT AACCAAAATC ATCCCAAACT
9841 TCCCACCCCA TACCCTATTA CCACTGCCAA TTACCTAGTG GTTTCATTTA CTCTAAACCT
9901 GTGATTCCTC TGAATTATTT TCATTTTAAA GAAATTGTAT TTGTTAAATA TGTACTACAA
9961 ACTTAGT
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